Restriction map for
pF1KB6181
(Circular) (Six-base) MAPSORT of: pF1KB6181.seq Check: 7152 from: 1 to: 5090
pF1KB6181 5090 bp
With 182 enzymes: SgfI *
July 28, 2009 13:23 ..
AatII G_ACGT'C
Cuts at: 1596 1596
Size: 5090
AccI GT'mk_AC
Cuts at: 1916 2128 1916
Size: 212 4878
Acc65I G'GTAC_C
Cuts at: 2106 2106
Size: 5090
AclI AA'CG_TT
Cuts at: 4930 4930
Size: 5090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1321 1753 2915 3347 4094 4362 102
Size: 1219 432 1162 432 747 268 830
Fragments arranged by size:
1219 1162 830 747 432 432 268
AflIII A'CryG_T
Cuts at: 2442 3546 4523 2442
Size: 1104 977 3009
Fragments arranged by size:
3009 1104 977
AgeI A'CCGG_T
Cuts at: 3478 3478
Size: 5090
AlwNI CAG_nnn'CTG
Cuts at: 318 2395 3962 318
Size: 2077 1567 1446
Fragments arranged by size:
2077 1567 1446
ApaI G_GGCC'C
Cuts at: 796 796
Size: 5090
ApaLI G'TGCA_C
Cuts at: 3860 3860
Size: 5090
ApoI r'AATT_y
Cuts at: 691 1715 2044 2094 4443 4699 691
Size: 1024 329 50 2349 256 1082
Fragments arranged by size:
2349 1082 1024 329 256 50
AseI AT'TA_AT
Cuts at: 20 1288 20
Size: 1268 3822
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
AvaI C'yCGr_G
Cuts at: 2110 2110
Size: 5090
BamHI G'GATC_C
Cuts at: 2115 2115
Size: 5090
BanI G'GyrC_C
Cuts at: 157 194 2106 2520 2768 2803 157
Size: 37 1912 414 248 35 2444
Fragments arranged by size:
2444 1912 414 248 37 35
BanII G_rGCy'C
Cuts at: 789 796 2104 3134 789
Size: 7 1308 1030 2745
Fragments arranged by size:
2745 1308 1030 7
BbeI G_GCGC'C
Cuts at: 198 2524 2772 198
Size: 2326 248 2516
Fragments arranged by size:
2516 2326 248
BbsI GAAGACnn'nnnn_
Cuts at: 858 984 858
Size: 126 4964
BciVI GTATCCnnnnn_n'
Cuts at: 2988 3749 5090 2988
Size: 761 1341 2988
Fragments arranged by size:
2988 1341 761
BclI T'GATC_A
Cuts at: 1434 2610 1434
Size: 1176 3914
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5090
BglII A'GATC_T
Cuts at: 2605 4642 2605
Size: 2037 3053
BlpI GC'TnA_GC
Cuts at: 2202 2202
Size: 5090
Bme1580I G_kGCm'C
Cuts at: 796 2715 2808 3864 796
Size: 1919 93 1056 2022
Fragments arranged by size:
2022 1919 1056 93
BmgBI CAC'GTC
Cuts at: 1920 1920
Size: 5090
BmrI ACTGGGnnnn_n'
Cuts at: 1869 2302 2383 2718 4862 1869
Size: 433 81 335 2144 2097
Fragments arranged by size:
2144 2097 433 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 595 2097 3457 595
Size: 1502 1360 2228
Fragments arranged by size:
2228 1502 1360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1418
Size: 5090
Bpu10I CC'TnA_GC
Cuts at: 788 788
Size: 5090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 1129 1196 2266 3145 3637 3935 285
Size: 844 67 1070 879 492 298 1440
Fragments arranged by size:
1440 1070 879 844 492 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 1344 1472 1632 4794 1344
Size: 128 160 3162 1640
Fragments arranged by size:
3162 1640 160 128
BsaAI yAC'GTr
Cuts at: 2443 2443
Size: 5090
BsaBI GATnn'nnATC
Cuts at: 6 1310 2604 4641 6
Size: 1304 1294 2037 455
Fragments arranged by size:
2037 1304 1294 455
BsaHI Gr'CG_yC
Cuts at: 195 1593 2521 2769 3471 4967 195
Size: 1398 928 248 702 1496 318
Fragments arranged by size:
1496 1398 928 702 318 248
BsaWI w'CCGG_w
Cuts at: 2279 2800 3478 3752 3899 4310 2279
Size: 521 678 274 147 411 3059
Fragments arranged by size:
3059 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1358 1388 1358
Size: 30 5060
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 135 613 135
Size: 478 4612
BseYI C'CCAG_C
Cuts at: 795 1237 1787 2515 3273 3850 4346 795
Size: 442 550 728 758 577 496 1539
Fragments arranged by size:
1539 758 728 577 550 496 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1925 196
Size: 1729 3361
BsiEI CG_ry'CG
Cuts at: 104 2632 2678 3886 104
Size: 2528 46 1208 1308
Fragments arranged by size:
2528 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 789 2104 2882 3072 3864 789
Size: 1315 778 190 792 2015
Fragments arranged by size:
2015 1315 792 778 190
BsmI GAATG_Cn'
Cuts at: 1199 1900 1199
Size: 701 4389
Bsp1286I G_dGCh'C
Cuts at: 789 796 2104 2715 2808 2882 3072 3134 3864
Size: 7 1308 611 93 74 190 62 730
Cuts at: 3864 789
Size: 2015
Fragments arranged by size:
2015 1308 730 611 190 93 74 62
7
BspEI T'CCGG_A
Cuts at: 4310 4310
Size: 5090
BspHI T'CATG_A
Cuts at: 2004 4266 2004
Size: 2262 2828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2024 2140 2354 2656 3037 2024
Size: 116 214 302 381 4077
Fragments arranged by size:
4077 381 302 214 116
BsrBI CCG'CTC
Cuts at: 50 3382 3436 4920 5085 50
Size: 3332 54 1484 165 55
Fragments arranged by size:
3332 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3002 3002
Size: 5090
BsrFI r'CCGG_y
Cuts at: 1261 3088 3269 3478 1261
Size: 1827 181 209 2873
Fragments arranged by size:
2873 1827 209 181
BssSI C'ACGA_G
Cuts at: 3361 3719 3361
Size: 358 4732
BstAPI GCAn_nnn'nTGC
Cuts at: 2467 2467
Size: 5090
BstBI TT'CG_AA
Cuts at: 849 3451 849
Size: 2602 2488
BstEII G'GTnAC_C
Cuts at: 1093 1093
Size: 5090
BstYI r'GATC_y
Cuts at: 1 2115 2582 2605 2940 3186 4198 4284 4296
Size: 2114 467 23 335 246 1012 86 12
Cuts at: 4296 4642 1
Size: 346 449
Fragments arranged by size:
2114 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5090
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 480 1861 3040 3221 3384 480
Size: 1381 1179 181 163 2186
Fragments arranged by size:
2186 1381 1179 181 163
BtsI GCAGTG_nn'
Cuts at: 2333 2333
Size: 5090
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1071 1106 1071
Size: 35 5055
DraI TTT'AAA
Cuts at: 1310 2089 1310
Size: 779 4311
DrdI GACnn_nn'nnGTC
Cuts at: 2796 3654 2796
Size: 858 4232
EaeI y'GGCC_r
Cuts at: 1259 2675 2849 3240 3267 4612 1259
Size: 1416 174 391 27 1345 1737
Fragments arranged by size:
1737 1416 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2675 2675
Size: 5090
EarI CTCTTCn'nnn_
Cuts at: 1972 3113 3323 1972
Size: 1141 210 3739
Fragments arranged by size:
3739 1141 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3298 3608 3754 4900 3298
Size: 310 146 1146 3488
Fragments arranged by size:
3488 1146 310 146
EcoICRI GAG'CTC
Cuts at: 787 2102 787
Size: 1315 3775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1321 1418 1753 2915 3347 4094 4362 102
Size: 1219 97 335 1162 432 747 268 830
Fragments arranged by size:
1219 1162 830 747 432 335 268 97
EcoNI CCTnn'n_nnAGG
Cuts at: 595 1610 595
Size: 1015 4075
EcoO109I rG'GnC_Cy
Cuts at: 792 2229 792
Size: 1437 3653
EcoRI G'AATT_C
Cuts at: 2044 2094 2044
Size: 50 5040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 1758 3109 498
Size: 1260 1351 2479
Fragments arranged by size:
2479 1351 1260
FspI TGC'GCA
Cuts at: 2871 2871
Size: 5090
HaeII r_GCGC'y
Cuts at: 198 2524 2772 3794 4773 198
Size: 2326 248 1022 979 515
Fragments arranged by size:
2326 1022 979 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 470 502 1358 1390 2932 2962 2964 2994 470
Size: 32 856 32 1542 30 2 30 2566
Fragments arranged by size:
2566 1542 856 32 32 30 30 2
HincII GTy'rAC
Cuts at: 770 1917 2129 770
Size: 1147 212 3731
Fragments arranged by size:
3731 1147 212
HindIII A'AGCT_T
Cuts at: 611 611
Size: 5090
HpaI GTT'AAC
Cuts at: 770 770
Size: 5090
KasI G'GCGC_C
Cuts at: 194 2520 2768 194
Size: 2326 248 2516
Fragments arranged by size:
2516 2326 248
KpnI G_GTAC'C
Cuts at: 2110 2110
Size: 5090
MfeI C'AATT_G
Cuts at: 779 779
Size: 5090
MluI A'CGCG_T
Cuts at: 4523 4523
Size: 5090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3761 3945 3761
Size: 184 4906
MscI TGG'CCA
Cuts at: 2851 2851
Size: 5090
MslI CAynn'nnrTG
Cuts at: 2024 3079 3206 2024
Size: 1055 127 3908
Fragments arranged by size:
3908 1055 127
MspA1I CmG'CkG
Cuts at: 669 2201 2515 2875 3888 4133 669
Size: 1532 314 360 1013 245 1626
Fragments arranged by size:
1626 1532 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3271 3271
Size: 5090
NarI GG'CG_CC
Cuts at: 195 2521 2769 195
Size: 2326 248 2516
Fragments arranged by size:
2516 2326 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5090
NdeI CA'TA_TG
Cuts at: 2021 2021
Size: 5090
NgoMIV G'CCGG_C
Cuts at: 3269 3269
Size: 5090
NspI r_CATG'y
Cuts at: 2143 2643 3550 2143
Size: 500 907 3683
Fragments arranged by size:
3683 907 500
PciI A'CATG_T
Cuts at: 3546 3546
Size: 5090
PmeI GTTT'AAAC
Cuts at: 2089 2089
Size: 5090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 5058
PsiI TTA'TAA
Cuts at: 626 1426 626
Size: 800 4290
PspOMI G'GGCC_C
Cuts at: 792 792
Size: 5090
PstI C_TGCA'G
Cuts at: 2137 2822 2137
Size: 685 4405
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5090
PvuII CAG'CTG
Cuts at: 669 2515 2875 669
Size: 1846 360 2884
Fragments arranged by size:
2884 1846 360
RsrII CG'GwC_CG
Cuts at: 3285 3285
Size: 5090
SacI G_AGCT'C
Cuts at: 789 2104 789
Size: 1315 3775
SalI G'TCGA_C
Cuts at: 1915 2127 1915
Size: 212 4878
SapI GCTCTTCn'nnn_
Cuts at: 3113 3323 3113
Size: 210 4880
SbfI CC_TGCA'GG
Cuts at: 2137 2137
Size: 5090
ScaI AGT'ACT
Cuts at: 127 1298 1730 2011 4607 127
Size: 1171 432 281 2596 610
Fragments arranged by size:
2596 1171 610 432 281
SexAI A'CCwGG_T
Cuts at: 1389 1389
Size: 5090
SfcI C'TryA_G
Cuts at: 33 2133 2818 3811 4002 33
Size: 2100 685 993 191 1121
Fragments arranged by size:
2100 1121 993 685 191
SfoI GGC'GCC
Cuts at: 196 2522 2770 196
Size: 2326 248 2516
Fragments arranged by size:
2516 2326 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
SmaI CCC'GGG
Cuts at: 2112 2112
Size: 5090
SmlI C'TyrA_G
Cuts at: 300 1144 1211 2245 3160 3652 3914 300
Size: 844 67 1034 915 492 262 1476
Fragments arranged by size:
1476 1034 915 844 492 262 67
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5090
SphI G_CATG'C
Cuts at: 2143 2643 2143
Size: 500 4590
StyI C'CwwG_G
Cuts at: 107 1653 2224 2578 107
Size: 1546 571 354 2619
Fragments arranged by size:
2619 1546 571 354
SwaI ATTT'AAAT
Cuts at: 1310 1310
Size: 5090
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2673 2806 3265 3474 2673
Size: 133 459 209 4289
Fragments arranged by size:
4289 459 209 133
TatI w'GTAC_w
Cuts at: 125 1150 1296 1368 1728 2009 4605 125
Size: 1025 146 72 360 281 2596 610
Fragments arranged by size:
2596 1025 610 360 281 146 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4607 4639 4607
Size: 32 5058
Tth111I GACn'n_nGTC
Cuts at: 1750 2887 1750
Size: 1137 3953
XbaI T'CTAG_A
Cuts at: 1167 2121 1167
Size: 954 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2585 2585
Size: 5090
XmaI C'CCGG_G
Cuts at: 2110 2110
Size: 5090
XmnI GAAnn'nnTTC
Cuts at: 469 1545 469
Size: 1076 4014
ZraI GAC'GTC
Cuts at: 1594 1594
Size: 5090
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI XhoI