Restriction map for
pF1KB6183
(Circular) (Six-base) MAPSORT of: pF1KB6183.seq Check: 5350 from: 1 to: 5090
pF1KB6183 5090 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 407 1860 407
Size: 1453 3637
AatII G_ACGT'C
Cuts at: 661 661
Size: 5090
AccI GT'mk_AC
Cuts at: 198 2128 198
Size: 1930 3160
Acc65I G'GTAC_C
Cuts at: 2106 2106
Size: 5090
AclI AA'CG_TT
Cuts at: 4930 4930
Size: 5090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 1601 1918 2915 3347 4094 4362 633
Size: 968 317 997 432 747 268 1361
Fragments arranged by size:
1361 997 968 747 432 317 268
AfeI AGC'GCT
Cuts at: 1222 1222
Size: 5090
AflIII A'CryG_T
Cuts at: 2442 3546 4523 2442
Size: 1104 977 3009
Fragments arranged by size:
3009 1104 977
AgeI A'CCGG_T
Cuts at: 3478 3478
Size: 5090
AhdI GACnn_n'nnGTC
Cuts at: 864 864
Size: 5090
AleI CACnn'nnGTG
Cuts at: 160 622 1330 160
Size: 462 708 3920
Fragments arranged by size:
3920 708 462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 236 1348 236
Size: 1112 3978
AlwNI CAG_nnn'CTG
Cuts at: 399 420 1005 1065 1272 1425 1579 1618 2395
Size: 21 585 60 207 153 154 39 777
Cuts at: 2395 3962 399
Size: 1567 1527
Fragments arranged by size:
1567 1527 777 585 207 154 153 60
39 21
ApaI G_GGCC'C
Cuts at: 170 170
Size: 5090
ApaLI G'TGCA_C
Cuts at: 3860 3860
Size: 5090
ApoI r'AATT_y
Cuts at: 2094 4443 4699 2094
Size: 2349 256 2485
Fragments arranged by size:
2485 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5090
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
AvaI C'yCGr_G
Cuts at: 138 312 1193 2110 138
Size: 174 881 917 3118
Fragments arranged by size:
3118 917 881 174
BamHI G'GATC_C
Cuts at: 358 2115 358
Size: 1757 3333
BanI G'GyrC_C
Cuts at: 148 462 1324 2106 2520 2768 2803 148
Size: 314 862 782 414 248 35 2435
Fragments arranged by size:
2435 862 782 414 314 248 35
BanII G_rGCy'C
Cuts at: 170 329 1025 1126 1445 1745 2006 2104 3134
Size: 159 696 101 319 300 261 98 1030
Cuts at: 3134 170
Size: 2126
Fragments arranged by size:
2126 1030 696 319 300 261 159 101
98
BbeI G_GCGC'C
Cuts at: 152 2524 2772 152
Size: 2372 248 2470
Fragments arranged by size:
2470 2372 248
BbsI GAAGACnn'nnnn_
Cuts at: 1726 1785 1802 1726
Size: 59 17 5014
Fragments arranged by size:
5014 59 17
BbvCI CC'TCA_GC
Cuts at: 421 1886 421
Size: 1465 3625
BciVI GTATCCnnnnn_n'
Cuts at: 2988 3749 5090 2988
Size: 761 1341 2988
Fragments arranged by size:
2988 1341 761
BclI T'GATC_A
Cuts at: 2610 2610
Size: 5090
BglI GCCn_nnn'nGGC
Cuts at: 165 477 1942 165
Size: 312 1465 3313
Fragments arranged by size:
3313 1465 312
BglII A'GATC_T
Cuts at: 1490 2605 4642 1490
Size: 1115 2037 1938
Fragments arranged by size:
2037 1938 1115
BlpI GC'TnA_GC
Cuts at: 938 1365 2202 938
Size: 427 837 3826
Fragments arranged by size:
3826 837 427
Bme1580I G_kGCm'C
Cuts at: 170 783 1327 2715 2808 3864 170
Size: 613 544 1388 93 1056 1396
Fragments arranged by size:
1396 1388 1056 613 544 93
BmrI ACTGGGnnnn_n'
Cuts at: 2302 2383 2718 4862 2302
Size: 81 335 2144 2530
Fragments arranged by size:
2530 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1782 1812 3457 1782
Size: 30 1645 3415
Fragments arranged by size:
3415 1645 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 478 630 703 883 991 1015 1027 1168
Size: 291 152 73 180 108 24 12 141
Cuts at: 1168 1435 1759 1777 1909 187
Size: 267 324 18 132 3368
Fragments arranged by size:
3368 324 291 267 180 152 141 132
108 73 24 18 12
Bpu10I CC'TnA_GC
Cuts at: 421 1217 1886 421
Size: 796 669 3625
Fragments arranged by size:
3625 796 669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2266 3145 3637 3935 2266
Size: 879 492 298 3421
Fragments arranged by size:
3421 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1560 1765 4794 1560
Size: 205 3029 1856
Fragments arranged by size:
3029 1856 205
BsaAI yAC'GTr
Cuts at: 1332 1434 1699 2443 1332
Size: 102 265 744 3979
Fragments arranged by size:
3979 744 265 102
BsaBI GATnn'nnATC
Cuts at: 6 2604 4641 6
Size: 2598 2037 455
Fragments arranged by size:
2598 2037 455
BsaHI Gr'CG_yC
Cuts at: 149 658 1355 2521 2769 3471 4967 149
Size: 509 697 1166 248 702 1496 272
Fragments arranged by size:
1496 1166 702 697 509 272 248
BsaWI w'CCGG_w
Cuts at: 1949 2030 2279 2800 3478 3752 3899 4310 1949
Size: 81 249 521 678 274 147 411 2729
Fragments arranged by size:
2729 678 521 411 274 249 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 556 586 1078 1108 556
Size: 30 492 30 4538
Fragments arranged by size:
4538 492 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 337 397 1165 1270 1579 1648 2086 334
Size: 3 60 768 105 309 69 438 3338
Fragments arranged by size:
3338 768 438 309 105 69 60 3
BseYI C'CCAG_C
Cuts at: 452 577 880 1265 1572 2515 3273 3850 4346
Size: 125 303 385 307 943 758 577 496
Cuts at: 4346 452
Size: 1196
Fragments arranged by size:
1196 943 758 577 496 385 307 303
125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 567 1456 385
Size: 182 889 4019
Fragments arranged by size:
4019 889 182
BsiEI CG_ry'CG
Cuts at: 104 2632 2678 3886 104
Size: 2528 46 1208 1308
Fragments arranged by size:
2528 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 329 1320 1445 1571 1765 2104 2882 3072
Size: 99 991 125 126 194 339 778 190
Cuts at: 3072 3864 230
Size: 792 1456
Fragments arranged by size:
1456 991 792 778 339 194 190 126
125 99
BsmI GAATG_Cn'
Cuts at: 1411 1411
Size: 5090
BsmBI CGTCTCn'nnnn_
Cuts at: 529 632 1282 529
Size: 103 650 4337
Fragments arranged by size:
4337 650 103
Bsp1286I G_dGCh'C
Cuts at: 170 230 329 783 1025 1126 1320 1327 1445
Size: 60 99 454 242 101 194 7 118
Cuts at: 1445 1571 1745 1765 2006 2104 2715 2808 2882
Size: 126 174 20 241 98 611 93 74
Cuts at: 2882 3072 3134 3864 170
Size: 190 62 730 1396
Fragments arranged by size:
1396 730 611 454 242 241 194 190
174 126 118 101 99 98 93 74
62 60 20 7
BspEI T'CCGG_A
Cuts at: 2030 4310 2030
Size: 2280 2810
BspHI T'CATG_A
Cuts at: 1718 4266 1718
Size: 2548 2542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 407 497 1860 2140 2354 2656 3037 407
Size: 90 1363 280 214 302 381 2460
Fragments arranged by size:
2460 1363 381 302 280 214 90
BsrBI CCG'CTC
Cuts at: 50 1364 3382 3436 4920 5085 50
Size: 1314 2018 54 1484 165 55
Fragments arranged by size:
2018 1484 1314 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3002 3002
Size: 5090
BsrFI r'CCGG_y
Cuts at: 3088 3269 3478 3088
Size: 181 209 4700
Fragments arranged by size:
4700 209 181
BssSI C'ACGA_G
Cuts at: 506 1804 3361 3719 506
Size: 1298 1557 358 1877
Fragments arranged by size:
1877 1557 1298 358
BstAPI GCAn_nnn'nTGC
Cuts at: 399 1425 2467 399
Size: 1026 1042 3022
Fragments arranged by size:
3022 1042 1026
BstBI TT'CG_AA
Cuts at: 3451 3451
Size: 5090
BstEII G'GTnAC_C
Cuts at: 1170 1917 1170
Size: 747 4343
BstXI CCAn_nnnn'nTGG
Cuts at: 114 162 1380 114
Size: 48 1218 3824
Fragments arranged by size:
3824 1218 48
BstYI r'GATC_y
Cuts at: 1 358 677 992 1490 2115 2582 2605 2940
Size: 357 319 315 498 625 467 23 335
Cuts at: 2940 3186 4198 4284 4296 4642 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 625 498 467 449 357 346 335
319 315 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1619 1619
Size: 5090
BtgI C'CryG_G
Cuts at: 107 161 231 330 1690 1937 107
Size: 54 70 99 1360 247 3260
Fragments arranged by size:
3260 1360 247 99 70 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1437 3040 3221 3384 1437
Size: 1603 181 163 3143
Fragments arranged by size:
3143 1603 181 163
BtsI GCAGTG_nn'
Cuts at: 783 1341 1938 2333 783
Size: 558 597 395 3540
Fragments arranged by size:
3540 597 558 395
DraI TTT'AAA
Cuts at: 2089 2089
Size: 5090
DraIII CAC_nnn'GTG
Cuts at: 1239 1239
Size: 5090
DrdI GACnn_nn'nnGTC
Cuts at: 181 2796 3654 181
Size: 2615 858 1617
Fragments arranged by size:
2615 1617 858
EaeI y'GGCC_r
Cuts at: 854 1370 1511 1693 1943 2675 2849 3240 3267
Size: 516 141 182 250 732 174 391 27
Cuts at: 3267 4612 854
Size: 1345 1332
Fragments arranged by size:
1345 1332 732 516 391 250 182 174
141 27
EagI C'GGCC_G
Cuts at: 2675 2675
Size: 5090
EarI CTCTTCn'nnn_
Cuts at: 1610 1649 1673 1803 1814 3113 3323 1610
Size: 39 24 130 11 1299 210 3377
Fragments arranged by size:
3377 1299 210 130 39 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 430 985 1598 3298 3608 3754 4900 430
Size: 555 613 1700 310 146 1146 620
Fragments arranged by size:
1700 1146 620 613 555 310 146
EcoICRI GAG'CTC
Cuts at: 327 1443 2102 327
Size: 1116 659 3315
Fragments arranged by size:
3315 1116 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 478 630 633 703 883 991 1015 1027
Size: 291 152 3 70 180 108 24 12
Cuts at: 1027 1168 1435 1601 1759 1777 1909 1918 2915
Size: 141 267 166 158 18 132 9 997
Cuts at: 2915 3347 4094 4362 187
Size: 432 747 268 915
Fragments arranged by size:
997 915 747 432 291 268 267 180
166 158 152 141 132 108 70 24
18 12 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1417 1417
Size: 5090
EcoO109I rG'GnC_Cy
Cuts at: 166 167 309 899 1422 1882 2229 166
Size: 1 142 590 523 460 347 3027
Fragments arranged by size:
3027 590 523 460 347 142 1
EcoRI G'AATT_C
Cuts at: 2094 2094
Size: 5090
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 5090
FspI TGC'GCA
Cuts at: 2871 2871
Size: 5090
HaeII r_GCGC'y
Cuts at: 152 887 1224 2524 2772 3794 4773 152
Size: 735 337 1300 248 1022 979 469
Fragments arranged by size:
1300 1022 979 735 469 337 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 850 882 1078 1110 1243 1275 2932
Size: 32 262 32 196 32 133 32 1657
Cuts at: 2932 2962 2964 2994 556
Size: 30 2 30 2652
Fragments arranged by size:
2652 1657 262 196 133 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 199 2129 199
Size: 1930 3160
KasI G'GCGC_C
Cuts at: 148 2520 2768 148
Size: 2372 248 2470
Fragments arranged by size:
2470 2372 248
KpnI G_GTAC'C
Cuts at: 2110 2110
Size: 5090
MluI A'CGCG_T
Cuts at: 4523 4523
Size: 5090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 935 3761 3945 935
Size: 2826 184 2080
Fragments arranged by size:
2826 2080 184
MscI TGG'CCA
Cuts at: 1945 2851 1945
Size: 906 4184
MslI CAynn'nnrTG
Cuts at: 160 622 1330 2063 3079 3206 160
Size: 462 708 733 1016 127 2044
Fragments arranged by size:
2044 1016 733 708 462 127
MspA1I CmG'CkG
Cuts at: 146 417 456 581 758 1083 1269 1369 1485
Size: 271 39 125 177 325 186 100 116
Cuts at: 1485 2201 2515 2875 3888 4133 146
Size: 716 314 360 1013 245 1103
Fragments arranged by size:
1103 1013 716 360 325 314 271 245
186 177 125 116 100 39
NaeI GCC'GGC
Cuts at: 3271 3271
Size: 5090
NarI GG'CG_CC
Cuts at: 149 2521 2769 149
Size: 2372 248 2470
Fragments arranged by size:
2470 2372 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5090
NgoMIV G'CCGG_C
Cuts at: 3269 3269
Size: 5090
NspI r_CATG'y
Cuts at: 740 1785 2143 2643 3550 740
Size: 1045 358 500 907 2280
Fragments arranged by size:
2280 1045 907 500 358
PasI CC'CwG_GG
Cuts at: 170 588 170
Size: 418 4672
PciI A'CATG_T
Cuts at: 3546 3546
Size: 5090
PflMI CCAn_nnn'nTGG
Cuts at: 1005 1335 1872 1914 1005
Size: 330 537 42 4181
Fragments arranged by size:
4181 537 330 42
PfoI T'CCnGG_A
Cuts at: 1964 1964
Size: 5090
PmeI GTTT'AAAC
Cuts at: 2089 2089
Size: 5090
PmlI CAC'GTG
Cuts at: 1332 1434 1332
Size: 102 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 5058
PpuMI rG'GwC_Cy
Cuts at: 899 899
Size: 5090
PspOMI G'GGCC_C
Cuts at: 166 166
Size: 5090
PstI C_TGCA'G
Cuts at: 254 1160 1490 1832 2137 2822 254
Size: 906 330 342 305 685 2522
Fragments arranged by size:
2522 906 685 342 330 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5090
PvuII CAG'CTG
Cuts at: 417 456 581 1269 1485 2515 2875 417
Size: 39 125 688 216 1030 360 2632
Fragments arranged by size:
2632 1030 688 360 216 125 39
RsrII CG'GwC_CG
Cuts at: 3285 3285
Size: 5090
SacI G_AGCT'C
Cuts at: 329 1445 2104 329
Size: 1116 659 3315
Fragments arranged by size:
3315 1116 659
SalI G'TCGA_C
Cuts at: 197 2127 197
Size: 1930 3160
SapI GCTCTTCn'nnn_
Cuts at: 1673 1814 3113 3323 1673
Size: 141 1299 210 3440
Fragments arranged by size:
3440 1299 210 141
SbfI CC_TGCA'GG
Cuts at: 2137 2137
Size: 5090
ScaI AGT'ACT
Cuts at: 4607 4607
Size: 5090
SfcI C'TryA_G
Cuts at: 33 250 1156 1486 1828 2133 2818 3811 4002
Size: 217 906 330 342 305 685 993 191
Cuts at: 4002 33
Size: 1121
Fragments arranged by size:
1121 993 906 685 342 330 305 217
191
SfoI GGC'GCC
Cuts at: 150 2522 2770 150
Size: 2372 248 2470
Fragments arranged by size:
2470 2372 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
SmaI CCC'GGG
Cuts at: 140 1195 2112 140
Size: 1055 917 3118
Fragments arranged by size:
3118 1055 917
SmlI C'TyrA_G
Cuts at: 2245 3160 3652 3914 2245
Size: 915 492 262 3421
Fragments arranged by size:
3421 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5090
SphI G_CATG'C
Cuts at: 2143 2643 2143
Size: 500 4590
StuI AGG'CCT
Cuts at: 1960 1960
Size: 5090
StyI C'CwwG_G
Cuts at: 107 1748 1856 2224 2578 107
Size: 1641 108 368 354 2619
Fragments arranged by size:
2619 1641 368 354 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 634 1103 1189 1342 1537 1586 2673 2806 3265
Size: 469 86 153 195 49 1087 133 459
Cuts at: 3265 3474 634
Size: 209 2250
Fragments arranged by size:
2250 1087 469 459 209 195 153 133
86 49
TatI w'GTAC_w
Cuts at: 626 4605 626
Size: 3979 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4607 4639 4607
Size: 32 5058
Tth111I GACn'n_nGTC
Cuts at: 2887 2887
Size: 5090
XbaI T'CTAG_A
Cuts at: 2121 2121
Size: 5090
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 585 1734 2585 585
Size: 1149 851 3090
Fragments arranged by size:
3090 1149 851
XmaI C'CCGG_G
Cuts at: 138 1193 2110 138
Size: 1055 917 3118
Fragments arranged by size:
3118 1055 917
ZraI GAC'GTC
Cuts at: 659 659
Size: 5090
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BcgI BfrBI BmgBI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI