Restriction map for
pF1KB6184
(Circular) (Six-base) MAPSORT of: pF1KB6184.seq Check: 2700 from: 1 to: 5213
pF1KB6184 5213 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2082 2082
Size: 5213
AccI GT'mk_AC
Cuts at: 2251 2251
Size: 5213
Acc65I G'GTAC_C
Cuts at: 2229 2229
Size: 5213
AclI AA'CG_TT
Cuts at: 5053 5053
Size: 5213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1055 1316 1723 2191 3038 3470 4217 4485
Size: 589 261 407 468 847 432 747 268
Cuts at: 4485 466
Size: 1194
Fragments arranged by size:
1194 847 747 589 468 432 407 268
261
AflIII A'CryG_T
Cuts at: 2565 3669 4646 2565
Size: 1104 977 3132
Fragments arranged by size:
3132 1104 977
AgeI A'CCGG_T
Cuts at: 3601 3601
Size: 5213
AleI CACnn'nnGTG
Cuts at: 1537 1537
Size: 5213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 256 288 256
Size: 32 5181
AlwNI CAG_nnn'CTG
Cuts at: 1476 2093 2160 2518 4085 1476
Size: 617 67 358 1567 2604
Fragments arranged by size:
2604 1567 617 358 67
ApaI G_GGCC'C
Cuts at: 240 240
Size: 5213
ApaLI G'TGCA_C
Cuts at: 3983 3983
Size: 5213
ApoI r'AATT_y
Cuts at: 922 1087 2217 4566 4822 922
Size: 165 1130 2349 256 1313
Fragments arranged by size:
2349 1313 1130 256 165
AseI AT'TA_AT
Cuts at: 20 20
Size: 5213
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5213
AvaI C'yCGr_G
Cuts at: 724 1801 2026 2233 724
Size: 1077 225 207 3704
Fragments arranged by size:
3704 1077 225 207
BamHI G'GATC_C
Cuts at: 2238 2238
Size: 5213
BanI G'GyrC_C
Cuts at: 1200 2229 2643 2891 2926 1200
Size: 1029 414 248 35 3487
Fragments arranged by size:
3487 1029 414 248 35
BanII G_rGCy'C
Cuts at: 240 725 875 914 1309 1519 1840 2227 3257
Size: 485 150 39 395 210 321 387 1030
Cuts at: 3257 240
Size: 2196
Fragments arranged by size:
2196 1030 485 395 387 321 210 150
39
BbeI G_GCGC'C
Cuts at: 2647 2895 2647
Size: 248 4965
BbsI GAAGACnn'nnnn_
Cuts at: 383 769 1991 2104 383
Size: 386 1222 113 3492
Fragments arranged by size:
3492 1222 386 113
BbvCI CC'TCA_GC
Cuts at: 232 232
Size: 5213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1281 1315 1281
Size: 34 5179
BciVI GTATCCnnnnn_n'
Cuts at: 3111 3872 5213 3111
Size: 761 1341 3111
Fragments arranged by size:
3111 1341 761
BclI T'GATC_A
Cuts at: 2733 2733
Size: 5213
BglI GCCn_nnn'nGGC
Cuts at: 1229 1229
Size: 5213
BglII A'GATC_T
Cuts at: 885 2728 4765 885
Size: 1843 2037 1333
Fragments arranged by size:
2037 1843 1333
BlpI GC'TnA_GC
Cuts at: 2325 2325
Size: 5213
Bme1580I G_kGCm'C
Cuts at: 240 753 1205 2838 2931 3987 240
Size: 513 452 1633 93 1056 1466
Fragments arranged by size:
1633 1466 1056 513 452 93
BmrI ACTGGGnnnn_n'
Cuts at: 697 1199 1464 1531 2425 2506 2841 4985 697
Size: 502 265 67 894 81 335 2144 925
Fragments arranged by size:
2144 925 894 502 335 265 81 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1536 3580 1536
Size: 2044 3169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 739 1531 1705 1750 727
Size: 12 792 174 45 4190
Fragments arranged by size:
4190 792 174 45 12
Bpu10I CC'TnA_GC
Cuts at: 232 1249 232
Size: 1017 4196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 1321 2389 3268 3760 4058 696
Size: 625 1068 879 492 298 1851
Fragments arranged by size:
1851 1068 879 625 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4917 4917
Size: 5213
BsaAI yAC'GTr
Cuts at: 2566 2566
Size: 5213
BsaBI GATnn'nnATC
Cuts at: 6 1279 2727 4764 6
Size: 1273 1448 2037 455
Fragments arranged by size:
2037 1448 1273 455
BsaHI Gr'CG_yC
Cuts at: 2644 2892 3594 5090 2644
Size: 248 702 1496 2767
Fragments arranged by size:
2767 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1853 2402 2923 3601 3875 4022 4433 1853
Size: 549 521 678 274 147 411 2633
Fragments arranged by size:
2633 678 549 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 778 808 778
Size: 30 5183
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 541 544 832 847 994 1747 232
Size: 309 3 288 15 147 753 3698
Fragments arranged by size:
3698 753 309 288 147 15 3
BseYI C'CCAG_C
Cuts at: 456 564 575 752 1288 2176 2638 3396 3973
Size: 108 11 177 536 888 462 758 577
Cuts at: 3973 4469 456
Size: 496 1200
Fragments arranged by size:
1200 888 758 577 536 496 462 177
108 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1162 1558 1563 1579 130
Size: 1032 396 5 16 3764
Fragments arranged by size:
3764 1032 396 16 5
BsiEI CG_ry'CG
Cuts at: 104 2755 2801 4009 104
Size: 2651 46 1208 1308
Fragments arranged by size:
2651 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 725 1337 1438 2227 3005 3195 3987 725
Size: 612 101 789 778 190 792 1951
Fragments arranged by size:
1951 792 789 778 612 190 101
Bsp1286I G_dGCh'C
Cuts at: 240 725 753 875 914 1205 1309 1337 1438
Size: 485 28 122 39 291 104 28 101
Cuts at: 1438 1519 1840 2227 2838 2931 3005 3195 3257
Size: 81 321 387 611 93 74 190 62
Cuts at: 3257 3987 240
Size: 730 1466
Fragments arranged by size:
1466 730 611 485 387 321 291 190
122 104 101 93 81 74 62 39
28 28
BspEI T'CCGG_A
Cuts at: 1853 4433 1853
Size: 2580 2633
BspHI T'CATG_A
Cuts at: 4389 4389
Size: 5213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2082 2263 2477 2779 3160 2082
Size: 181 214 302 381 4135
Fragments arranged by size:
4135 381 302 214 181
BsrBI CCG'CTC
Cuts at: 50 3505 3559 5043 5208 50
Size: 3455 54 1484 165 55
Fragments arranged by size:
3455 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 316 3125 316
Size: 2809 2404
BsrFI r'CCGG_y
Cuts at: 452 3211 3392 3601 452
Size: 2759 181 209 2064
Fragments arranged by size:
2759 2064 209 181
BssSI C'ACGA_G
Cuts at: 3484 3842 3484
Size: 358 4855
BstAPI GCAn_nnn'nTGC
Cuts at: 545 1173 2590 545
Size: 628 1417 3168
Fragments arranged by size:
3168 1417 628
BstBI TT'CG_AA
Cuts at: 3574 3574
Size: 5213
BstEII G'GTnAC_C
Cuts at: 1560 1560
Size: 5213
BstYI r'GATC_y
Cuts at: 1 132 885 2238 2705 2728 3063 3309 4321
Size: 131 753 1353 467 23 335 246 1012
Cuts at: 4321 4407 4419 4765 1
Size: 86 12 346 449
Fragments arranged by size:
1353 1012 753 467 449 346 335 246
131 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 500 1100 500
Size: 600 4613
BtgI C'CryG_G
Cuts at: 361 758 1640 361
Size: 397 882 3934
Fragments arranged by size:
3934 882 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3163 3344 3507 3163
Size: 181 163 4869
Fragments arranged by size:
4869 181 163
BtsI GCAGTG_nn'
Cuts at: 427 472 902 1570 1909 2045 2456 427
Size: 45 430 668 339 136 411 3184
Fragments arranged by size:
3184 668 430 411 339 136 45
DraI TTT'AAA
Cuts at: 2212 2212
Size: 5213
DraIII CAC_nnn'GTG
Cuts at: 561 561
Size: 5213
DrdI GACnn_nn'nnGTC
Cuts at: 385 2919 3777 385
Size: 2534 858 1821
Fragments arranged by size:
2534 1821 858
EaeI y'GGCC_r
Cuts at: 278 1230 2798 2972 3363 3390 4735 278
Size: 952 1568 174 391 27 1345 756
Fragments arranged by size:
1568 1345 952 756 391 174 27
EagI C'GGCC_G
Cuts at: 2798 2798
Size: 5213
EarI CTCTTCn'nnn_
Cuts at: 1345 1807 1959 3236 3446 1345
Size: 462 152 1277 210 3112
Fragments arranged by size:
3112 1277 462 210 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 132 1182 1848 3421 3731 3877 5023 132
Size: 1050 666 1573 310 146 1146 322
Fragments arranged by size:
1573 1146 1050 666 322 310 146
EcoICRI GAG'CTC
Cuts at: 723 2225 723
Size: 1502 3711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 727 739 1055 1316 1531 1705 1723 1750
Size: 261 12 316 261 215 174 18 27
Cuts at: 1750 2191 3038 3470 4217 4485 466
Size: 441 847 432 747 268 1194
Fragments arranged by size:
1194 847 747 441 432 316 268 261
261 215 174 27 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 352 1099 1732 352
Size: 747 633 3833
Fragments arranged by size:
3833 747 633
EcoO109I rG'GnC_Cy
Cuts at: 236 237 2352 236
Size: 1 2115 3097
Fragments arranged by size:
3097 2115 1
EcoRI G'AATT_C
Cuts at: 922 2217 922
Size: 1295 3918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 5213
FspI TGC'GCA
Cuts at: 2994 2994
Size: 5213
HaeII r_GCGC'y
Cuts at: 2647 2895 3917 4896 2647
Size: 248 1022 979 2964
Fragments arranged by size:
2964 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1093 1125 3055 3085 3087 3117 1093
Size: 32 1930 30 2 30 3189
Fragments arranged by size:
3189 1930 32 30 30 2
HincII GTy'rAC
Cuts at: 2252 2252
Size: 5213
KasI G'GCGC_C
Cuts at: 2643 2891 2643
Size: 248 4965
KpnI G_GTAC'C
Cuts at: 2233 2233
Size: 5213
MluI A'CGCG_T
Cuts at: 4646 4646
Size: 5213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1037 3884 4068 1037
Size: 2847 184 2182
Fragments arranged by size:
2847 2182 184
MscI TGG'CCA
Cuts at: 280 1232 2974 280
Size: 952 1742 2519
Fragments arranged by size:
2519 1742 952
MslI CAynn'nnrTG
Cuts at: 1537 3202 3329 1537
Size: 1665 127 3421
Fragments arranged by size:
3421 1665 127
MspA1I CmG'CkG
Cuts at: 231 575 1015 1146 2324 2638 2998 4011 4256
Size: 344 440 131 1178 314 360 1013 245
Cuts at: 4256 231
Size: 1188
Fragments arranged by size:
1188 1178 1013 440 360 344 314 245
131
NaeI GCC'GGC
Cuts at: 454 3394 454
Size: 2940 2273
NarI GG'CG_CC
Cuts at: 2644 2892 2644
Size: 248 4965
NcoI C'CATG_G
Cuts at: 361 1640 361
Size: 1279 3934
NgoMIV G'CCGG_C
Cuts at: 452 3392 452
Size: 2940 2273
NspI r_CATG'y
Cuts at: 1609 2266 2766 3673 1609
Size: 657 500 907 3149
Fragments arranged by size:
3149 907 657 500
PciI A'CATG_T
Cuts at: 3669 3669
Size: 5213
PflMI CCAn_nnn'nTGG
Cuts at: 1554 2093 1554
Size: 539 4674
PfoI T'CCnGG_A
Cuts at: 1403 1403
Size: 5213
PmeI GTTT'AAAC
Cuts at: 2212 2212
Size: 5213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 5181
PspOMI G'GGCC_C
Cuts at: 236 236
Size: 5213
PspXI vC'TCGA_Gb
Cuts at: 724 724
Size: 5213
PstI C_TGCA'G
Cuts at: 1116 2260 2945 1116
Size: 1144 685 3384
Fragments arranged by size:
3384 1144 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5213
PvuII CAG'CTG
Cuts at: 231 1146 2638 2998 231
Size: 915 1492 360 2446
Fragments arranged by size:
2446 1492 915 360
RsrII CG'GwC_CG
Cuts at: 3408 3408
Size: 5213
SacI G_AGCT'C
Cuts at: 725 2227 725
Size: 1502 3711
SalI G'TCGA_C
Cuts at: 2250 2250
Size: 5213
SapI GCTCTTCn'nnn_
Cuts at: 3236 3446 3236
Size: 210 5003
SbfI CC_TGCA'GG
Cuts at: 2260 2260
Size: 5213
ScaI AGT'ACT
Cuts at: 1636 4730 1636
Size: 3094 2119
SfcI C'TryA_G
Cuts at: 33 1112 2256 2941 3934 4125 33
Size: 1079 1144 685 993 191 1121
Fragments arranged by size:
1144 1121 1079 993 685 191
SfoI GGC'GCC
Cuts at: 2645 2893 2645
Size: 248 4965
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5213
SmaI CCC'GGG
Cuts at: 2235 2235
Size: 5213
SmlI C'TyrA_G
Cuts at: 675 724 1336 2368 3283 3775 4037 675
Size: 49 612 1032 915 492 262 1851
Fragments arranged by size:
1851 1032 915 612 492 262 49
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5213
SphI G_CATG'C
Cuts at: 2266 2766 2266
Size: 500 4713
StyI C'CwwG_G
Cuts at: 361 914 1640 2347 2701 361
Size: 553 726 707 354 2873
Fragments arranged by size:
2873 726 707 553 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 538 972 1228 2796 2929 3388 3597 538
Size: 434 256 1568 133 459 209 2154
Fragments arranged by size:
2154 1568 459 434 256 209 133
TatI w'GTAC_w
Cuts at: 317 707 1634 4728 317
Size: 390 927 3094 802
Fragments arranged by size:
3094 927 802 390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 1341 1373 4730 4762 178
Size: 32 1131 32 3357 32 629
Fragments arranged by size:
3357 1131 629 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 349 3010 349
Size: 2661 2552
XbaI T'CTAG_A
Cuts at: 2244 2244
Size: 5213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2708 2708
Size: 5213
XhoI C'TCGA_G
Cuts at: 724 724
Size: 5213
XmaI C'CCGG_G
Cuts at: 2233 2233
Size: 5213
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI