Restriction map for
pF1KB6187
(Circular) (Six-base) MAPSORT of: pF1KB6187.seq Check: 6910 from: 1 to: 5093
pF1KB6187 5093 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1095 1095
Size: 5093
AccI GT'mk_AC
Cuts at: 2131 2131
Size: 5093
Acc65I G'GTAC_C
Cuts at: 1565 2109 1565
Size: 544 4549
AclI AA'CG_TT
Cuts at: 4933 4933
Size: 5093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1326 2047 2092 2918 3350 4097 4365 1326
Size: 721 45 826 432 747 268 2054
Fragments arranged by size:
2054 826 747 721 432 268 45
AflIII A'CryG_T
Cuts at: 217 2445 3549 4526 217
Size: 2228 1104 977 784
Fragments arranged by size:
2228 1104 977 784
AgeI A'CCGG_T
Cuts at: 3481 3481
Size: 5093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1999 1999
Size: 5093
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 310 342 310
Size: 32 5061
AlwNI CAG_nnn'CTG
Cuts at: 235 2398 3965 235
Size: 2163 1567 1363
Fragments arranged by size:
2163 1567 1363
ApaLI G'TGCA_C
Cuts at: 3863 3863
Size: 5093
ApoI r'AATT_y
Cuts at: 1018 2097 4446 4702 1018
Size: 1079 2349 256 1409
Fragments arranged by size:
2349 1409 1079 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5093
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
AvaI C'yCGr_G
Cuts at: 755 2113 755
Size: 1358 3735
BamHI G'GATC_C
Cuts at: 2118 2118
Size: 5093
BanI G'GyrC_C
Cuts at: 361 740 814 1565 1682 2109 2523 2771 2806
Size: 379 74 751 117 427 414 248 35
Cuts at: 2806 361
Size: 2648
Fragments arranged by size:
2648 751 427 414 379 248 117 74
35
BanII G_rGCy'C
Cuts at: 691 2107 3137 691
Size: 1416 1030 2647
Fragments arranged by size:
2647 1416 1030
BbeI G_GCGC'C
Cuts at: 2527 2775 2527
Size: 248 4845
BbsI GAAGACnn'nnnn_
Cuts at: 500 538 1332 500
Size: 38 794 4261
Fragments arranged by size:
4261 794 38
BbvCI CC'TCA_GC
Cuts at: 1728 1928 1728
Size: 200 4893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5059
BciVI GTATCCnnnnn_n'
Cuts at: 1113 2991 3752 5093 1113
Size: 1878 761 1341 1113
Fragments arranged by size:
1878 1341 1113 761
BclI T'GATC_A
Cuts at: 2613 2613
Size: 5093
BglI GCCn_nnn'nGGC
Cuts at: 1171 1171
Size: 5093
BglII A'GATC_T
Cuts at: 2608 4645 2608
Size: 2037 3056
BlpI GC'TnA_GC
Cuts at: 1161 2205 1161
Size: 1044 4049
Bme1580I G_kGCm'C
Cuts at: 366 2718 2811 3867 366
Size: 2352 93 1056 1592
Fragments arranged by size:
2352 1592 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2305 2386 2721 4865 2305
Size: 81 335 2144 2533
Fragments arranged by size:
2533 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1079 1395 3460 1079
Size: 316 2065 2712
Fragments arranged by size:
2712 2065 316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 1477 1734 1858 812
Size: 665 257 124 4047
Fragments arranged by size:
4047 665 257 124
Bpu10I CC'TnA_GC
Cuts at: 1728 1928 1728
Size: 200 4893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2029 2269 3148 3640 3938 1336
Size: 693 240 879 492 298 2491
Fragments arranged by size:
2491 879 693 492 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 4797 4797
Size: 5093
BsaAI yAC'GTr
Cuts at: 2446 2446
Size: 5093
BsaBI GATnn'nnATC
Cuts at: 6 2607 4644 6
Size: 2601 2037 455
Fragments arranged by size:
2601 2037 455
BsaHI Gr'CG_yC
Cuts at: 2524 2772 3474 4970 2524
Size: 248 702 1496 2647
Fragments arranged by size:
2647 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1116 2282 2803 3481 3755 3902 4313 1116
Size: 1166 521 678 274 147 411 1896
Fragments arranged by size:
1896 1166 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1092 1122 1092
Size: 30 5063
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1869 1869
Size: 5093
BseYI C'CCAG_C
Cuts at: 228 663 874 1156 1419 2031 2518 3276 3853
Size: 435 211 282 263 612 487 758 577
Cuts at: 3853 4349 228
Size: 496 972
Fragments arranged by size:
972 758 612 577 496 487 435 282
263 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2024 2024
Size: 5093
BsiEI CG_ry'CG
Cuts at: 104 2635 2681 3889 104
Size: 2531 46 1208 1308
Fragments arranged by size:
2531 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2107 2885 3075 3867 2107
Size: 778 190 792 3333
Fragments arranged by size:
3333 792 778 190
BsmI GAATG_Cn'
Cuts at: 507 507
Size: 5093
Bsp1286I G_dGCh'C
Cuts at: 366 691 2107 2718 2811 2885 3075 3137 3867
Size: 325 1416 611 93 74 190 62 730
Cuts at: 3867 366
Size: 1592
Fragments arranged by size:
1592 1416 730 611 325 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4313 4313
Size: 5093
BspHI T'CATG_A
Cuts at: 4269 4269
Size: 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 809 1095 2143 2357 2659 3040 809
Size: 286 1048 214 302 381 2862
Fragments arranged by size:
2862 1048 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 1610 3385 3439 4923 5088 50
Size: 1560 1775 54 1484 165 55
Fragments arranged by size:
1775 1560 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 3005 240
Size: 2765 2328
BsrFI r'CCGG_y
Cuts at: 1685 2066 3091 3272 3481 1685
Size: 381 1025 181 209 3297
Fragments arranged by size:
3297 1025 381 209 181
BssSI C'ACGA_G
Cuts at: 1963 3364 3722 1963
Size: 1401 358 3334
Fragments arranged by size:
3334 1401 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1745 2470 1745
Size: 725 4368
BstBI TT'CG_AA
Cuts at: 3454 3454
Size: 5093
BstEII G'GTnAC_C
Cuts at: 173 173
Size: 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 445 445
Size: 5093
BstYI r'GATC_y
Cuts at: 1 2118 2585 2608 2943 3189 4201 4287 4299
Size: 2117 467 23 335 246 1012 86 12
Cuts at: 4299 4645 1
Size: 346 449
Fragments arranged by size:
2117 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1362 1362
Size: 5093
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 572 1862 3043 3224 3387 572
Size: 1290 1181 181 163 2278
Fragments arranged by size:
2278 1290 1181 181 163
BtsI GCAGTG_nn'
Cuts at: 1532 1721 2004 2336 1532
Size: 189 283 332 4289
Fragments arranged by size:
4289 332 283 189
ClaI AT'CG_AT
Cuts at: 1970 1970
Size: 5093
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 318 330 353 365 1644 1679 318
Size: 12 23 12 1279 35 3732
Fragments arranged by size:
3732 1279 35 23 12 12
DraI TTT'AAA
Cuts at: 2092 2092
Size: 5093
DrdI GACnn_nn'nnGTC
Cuts at: 1524 2799 3657 1524
Size: 1275 858 2960
Fragments arranged by size:
2960 1275 858
EaeI y'GGCC_r
Cuts at: 1866 2678 2852 3243 3270 4615 1866
Size: 812 174 391 27 1345 2344
Fragments arranged by size:
2344 1345 812 391 174 27
EagI C'GGCC_G
Cuts at: 2678 2678
Size: 5093
EarI CTCTTCn'nnn_
Cuts at: 1024 3116 3326 1024
Size: 2092 210 2791
Fragments arranged by size:
2791 2092 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3301 3611 3757 4903 3301
Size: 310 146 1146 3491
Fragments arranged by size:
3491 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 5093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 1326 1477 1734 1858 2047 2092 2918 3350
Size: 514 151 257 124 189 45 826 432
Cuts at: 3350 4097 4365 812
Size: 747 268 1540
Fragments arranged by size:
1540 826 747 514 432 268 257 189
151 124 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1677 1677
Size: 5093
EcoO109I rG'GnC_Cy
Cuts at: 150 309 725 1036 1173 1216 1872 1980 2035
Size: 159 416 311 137 43 656 108 55
Cuts at: 2035 2232 150
Size: 197 3011
Fragments arranged by size:
3011 656 416 311 197 159 137 108
55 43
EcoRI G'AATT_C
Cuts at: 2097 2097
Size: 5093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3112 3112
Size: 5093
FspI TGC'GCA
Cuts at: 1738 1996 2874 1738
Size: 258 878 3957
Fragments arranged by size:
3957 878 258
HaeII r_GCGC'y
Cuts at: 235 2527 2775 3797 4776 235
Size: 2292 248 1022 979 552
Fragments arranged by size:
2292 1022 979 552 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 1630 1662 1777 1809 1864 1896 2935
Size: 32 1027 32 115 32 55 32 1039
Cuts at: 2935 2965 2967 2997 571
Size: 30 2 30 2667
Fragments arranged by size:
2667 1039 1027 115 55 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 423 1428 2132 423
Size: 1005 704 3384
Fragments arranged by size:
3384 1005 704
KasI G'GCGC_C
Cuts at: 2523 2771 2523
Size: 248 4845
KpnI G_GTAC'C
Cuts at: 1569 2113 1569
Size: 544 4549
MluI A'CGCG_T
Cuts at: 4526 4526
Size: 5093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 330 1805 3764 3948 330
Size: 1475 1959 184 1475
Fragments arranged by size:
1959 1475 1475 184
MscI TGG'CCA
Cuts at: 2854 2854
Size: 5093
MslI CAynn'nnrTG
Cuts at: 1367 2008 3082 3209 1367
Size: 641 1074 127 3251
Fragments arranged by size:
3251 1074 641 127
MspA1I CmG'CkG
Cuts at: 430 1006 1732 2204 2518 2878 3891 4136 430
Size: 576 726 472 314 360 1013 245 1387
Fragments arranged by size:
1387 1013 726 576 472 360 314 245
NaeI GCC'GGC
Cuts at: 3274 3274
Size: 5093
NarI GG'CG_CC
Cuts at: 2524 2772 2524
Size: 248 4845
NcoI C'CATG_G
Cuts at: 1362 1362
Size: 5093
NgoMIV G'CCGG_C
Cuts at: 3272 3272
Size: 5093
NspI r_CATG'y
Cuts at: 221 2146 2646 3553 221
Size: 1925 500 907 1761
Fragments arranged by size:
1925 1761 907 500
PasI CC'CwG_GG
Cuts at: 1819 1819
Size: 5093
PciI A'CATG_T
Cuts at: 217 3549 217
Size: 3332 1761
PflMI CCAn_nnn'nTGG
Cuts at: 487 487
Size: 5093
PfoI T'CCnGG_A
Cuts at: 1454 1913 1454
Size: 459 4634
PmeI GTTT'AAAC
Cuts at: 2092 2092
Size: 5093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 5061
PpuMI rG'GwC_Cy
Cuts at: 150 309 1036 1872 150
Size: 159 727 836 3371
Fragments arranged by size:
3371 836 727 159
PstI C_TGCA'G
Cuts at: 806 2140 2825 806
Size: 1334 685 3074
Fragments arranged by size:
3074 1334 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5093
PvuII CAG'CTG
Cuts at: 430 1006 1732 2518 2878 430
Size: 576 726 786 360 2645
Fragments arranged by size:
2645 786 726 576 360
RsrII CG'GwC_CG
Cuts at: 1827 3288 1827
Size: 1461 3632
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 5093
SalI G'TCGA_C
Cuts at: 2130 2130
Size: 5093
SanDI GG'GwC_CC
Cuts at: 1872 1872
Size: 5093
SapI GCTCTTCn'nnn_
Cuts at: 3116 3326 3116
Size: 210 4883
SbfI CC_TGCA'GG
Cuts at: 2140 2140
Size: 5093
ScaI AGT'ACT
Cuts at: 4610 4610
Size: 5093
SexAI A'CCwGG_T
Cuts at: 1572 1572
Size: 5093
SfcI C'TryA_G
Cuts at: 33 120 475 802 2136 2821 3814 4005 33
Size: 87 355 327 1334 685 993 191 1121
Fragments arranged by size:
1334 1121 993 685 355 327 191 87
SfoI GGC'GCC
Cuts at: 2525 2773 2525
Size: 248 4845
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
SmaI CCC'GGG
Cuts at: 2115 2115
Size: 5093
SmlI C'TyrA_G
Cuts at: 1351 2044 2248 3163 3655 3917 1351
Size: 693 204 915 492 262 2527
Fragments arranged by size:
2527 915 693 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5093
SphI G_CATG'C
Cuts at: 2146 2646 2146
Size: 500 4593
StuI AGG'CCT
Cuts at: 1306 1306
Size: 5093
StyI C'CwwG_G
Cuts at: 1362 1613 2227 2581 1362
Size: 251 614 354 3874
Fragments arranged by size:
3874 614 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 704 975 1844 2676 2809 3268 3477 704
Size: 271 869 832 133 459 209 2320
Fragments arranged by size:
2320 869 832 459 271 209 133
TatI w'GTAC_w
Cuts at: 1544 2015 4608 1544
Size: 471 2593 2029
Fragments arranged by size:
2593 2029 471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 5061
Tth111I GACn'n_nGTC
Cuts at: 2890 2890
Size: 5093
XbaI T'CTAG_A
Cuts at: 2124 2124
Size: 5093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 900 2588 900
Size: 1688 3405
XmaI C'CCGG_G
Cuts at: 2113 2113
Size: 5093
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI