Restriction map for
pF1KB6190
(Circular) (Six-base) MAPSORT of: pF1KB6190.seq Check: 2578 from: 1 to: 5090
pF1KB6190 5090 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 2128 2128
Size: 5090
Acc65I G'GTAC_C
Cuts at: 2106 2106
Size: 5090
AclI AA'CG_TT
Cuts at: 4930 4930
Size: 5090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 843 1038 1542 1626 2915 3347 4094 4362
Size: 318 195 504 84 1289 432 747 268
Cuts at: 4362 525
Size: 1253
Fragments arranged by size:
1289 1253 747 504 432 318 268 195
84
AflIII A'CryG_T
Cuts at: 460 590 1448 2442 3546 4523 460
Size: 130 858 994 1104 977 1027
Fragments arranged by size:
1104 1027 994 977 858 130
AgeI A'CCGG_T
Cuts at: 3478 3478
Size: 5090
AleI CACnn'nnGTG
Cuts at: 377 1576 377
Size: 1199 3891
AlwNI CAG_nnn'CTG
Cuts at: 453 2395 3962 453
Size: 1942 1567 1581
Fragments arranged by size:
1942 1581 1567
ApaLI G'TGCA_C
Cuts at: 456 3860 456
Size: 3404 1686
ApoI r'AATT_y
Cuts at: 1003 1172 1300 1340 1384 1636 1676 1888 2094
Size: 169 128 40 44 252 40 212 206
Cuts at: 2094 4443 4699 1003
Size: 2349 256 1394
Fragments arranged by size:
2349 1394 256 252 212 206 169 128
44 40 40
AseI AT'TA_AT
Cuts at: 20 908 20
Size: 888 4202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
AvaI C'yCGr_G
Cuts at: 179 2110 179
Size: 1931 3159
BamHI G'GATC_C
Cuts at: 2115 2115
Size: 5090
BanI G'GyrC_C
Cuts at: 294 2106 2520 2768 2803 294
Size: 1812 414 248 35 2581
Fragments arranged by size:
2581 1812 414 248 35
BanII G_rGCy'C
Cuts at: 2034 2104 3134 2034
Size: 70 1030 3990
Fragments arranged by size:
3990 1030 70
BbeI G_GCGC'C
Cuts at: 2524 2772 2524
Size: 248 4842
BbvCI CC'TCA_GC
Cuts at: 845 845
Size: 5090
BciVI GTATCCnnnnn_n'
Cuts at: 2988 3749 5090 2988
Size: 761 1341 2988
Fragments arranged by size:
2988 1341 761
BclI T'GATC_A
Cuts at: 2610 2610
Size: 5090
BfrBI ATG'CAT
Cuts at: 707 1370 707
Size: 663 4427
BglII A'GATC_T
Cuts at: 1105 2605 4642 1105
Size: 1500 2037 1553
Fragments arranged by size:
2037 1553 1500
BlpI GC'TnA_GC
Cuts at: 2202 2202
Size: 5090
Bme1580I G_kGCm'C
Cuts at: 460 2715 2808 3864 460
Size: 2255 93 1056 1686
Fragments arranged by size:
2255 1686 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2302 2383 2718 4862 2302
Size: 81 335 2144 2530
Fragments arranged by size:
2530 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3457 3457
Size: 5090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 714 1118 1286 1356 1538 1622 1706 1722
Size: 455 404 168 70 182 84 84 16
Cuts at: 1722 1874 1958 2042 259
Size: 152 84 84 3307
Fragments arranged by size:
3307 455 404 182 168 152 84 84
84 84 70 16
Bpu10I CC'TnA_GC
Cuts at: 845 845
Size: 5090
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 2266 3145 3637 3935 220
Size: 2046 879 492 298 1375
Fragments arranged by size:
2046 1375 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 846 4794 846
Size: 3948 1142
BsaAI yAC'GTr
Cuts at: 463 611 1449 1953 2443 463
Size: 148 838 504 490 3110
Fragments arranged by size:
3110 838 504 490 148
BsaBI GATnn'nnATC
Cuts at: 6 2604 4641 6
Size: 2598 2037 455
Fragments arranged by size:
2598 2037 455
BsaHI Gr'CG_yC
Cuts at: 2521 2769 3471 4967 2521
Size: 248 702 1496 2644
Fragments arranged by size:
2644 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2279 2800 3478 3752 3899 4310 2279
Size: 521 678 274 147 411 3059
Fragments arranged by size:
3059 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 217 199
Size: 18 5072
BseYI C'CCAG_C
Cuts at: 2515 3273 3850 4346 2515
Size: 758 577 496 3259
Fragments arranged by size:
3259 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 1305 1473 1557 1809 1893 1903 633
Size: 672 168 84 252 84 10 3820
Fragments arranged by size:
3820 672 252 168 84 84 10
BsiEI CG_ry'CG
Cuts at: 104 2632 2678 3886 104
Size: 2528 46 1208 1308
Fragments arranged by size:
2528 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 460 1334 2034 2104 2882 3072 3864 460
Size: 874 700 70 778 190 792 1686
Fragments arranged by size:
1686 874 792 778 700 190 70
BsmI GAATG_Cn'
Cuts at: 709 1372 709
Size: 663 4427
Bsp1286I G_dGCh'C
Cuts at: 460 1334 2034 2104 2715 2808 2882 3072 3134
Size: 874 700 70 611 93 74 190 62
Cuts at: 3134 3864 460
Size: 730 1686
Fragments arranged by size:
1686 874 730 700 611 190 93 74
70 62
BspEI T'CCGG_A
Cuts at: 4310 4310
Size: 5090
BspHI T'CATG_A
Cuts at: 4266 4266
Size: 5090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2140 2354 2656 3037 2140
Size: 214 302 381 4193
Fragments arranged by size:
4193 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3382 3436 4920 5085 50
Size: 3332 54 1484 165 55
Fragments arranged by size:
3332 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1713 3002 1713
Size: 1289 3801
BsrFI r'CCGG_y
Cuts at: 3088 3269 3478 3088
Size: 181 209 4700
Fragments arranged by size:
4700 209 181
BsrGI T'GTAC_A
Cuts at: 580 1665 580
Size: 1085 4005
BssSI C'ACGA_G
Cuts at: 2035 3361 3719 2035
Size: 1326 358 3406
Fragments arranged by size:
3406 1326 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2467 2467
Size: 5090
BstBI TT'CG_AA
Cuts at: 3451 3451
Size: 5090
BstYI r'GATC_y
Cuts at: 1 601 1105 1441 2115 2582 2605 2940 3186
Size: 600 504 336 674 467 23 335 246
Cuts at: 3186 4198 4284 4296 4642 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 674 600 504 467 449 346 336
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5090
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3040 3221 3384 3040
Size: 181 163 4746
Fragments arranged by size:
4746 181 163
BtsI GCAGTG_nn'
Cuts at: 1041 1125 1293 1545 1797 1881 2333 1041
Size: 84 168 252 252 84 452 3798
Fragments arranged by size:
3798 452 252 252 168 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1191 1226 1695 1730 1191
Size: 35 469 35 4551
Fragments arranged by size:
4551 469 35 35
DraI TTT'AAA
Cuts at: 2089 2089
Size: 5090
DrdI GACnn_nn'nnGTC
Cuts at: 348 2796 3654 348
Size: 2448 858 1784
Fragments arranged by size:
2448 1784 858
EaeI y'GGCC_r
Cuts at: 2675 2849 3240 3267 4612 2675
Size: 174 391 27 1345 3153
Fragments arranged by size:
3153 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2675 2675
Size: 5090
EarI CTCTTCn'nnn_
Cuts at: 568 824 3113 3323 568
Size: 256 2289 210 2335
Fragments arranged by size:
2335 2289 256 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3298 3608 3754 4900 3298
Size: 310 146 1146 3488
Fragments arranged by size:
3488 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2032 2102 2032
Size: 70 5020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 525 714 843 1038 1118 1286 1356 1538
Size: 266 189 129 195 80 168 70 182
Cuts at: 1538 1542 1622 1626 1706 1722 1874 1958 2042
Size: 4 80 4 80 16 152 84 84
Cuts at: 2042 2915 3347 4094 4362 259
Size: 873 432 747 268 987
Fragments arranged by size:
987 873 747 432 268 266 195 189
182 168 152 129 84 84 80 80
80 70 16 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 202 202
Size: 5090
EcoO109I rG'GnC_Cy
Cuts at: 560 1358 2229 560
Size: 798 871 3421
Fragments arranged by size:
3421 871 798
EcoRI G'AATT_C
Cuts at: 1172 1340 1676 2094 1172
Size: 168 336 418 4168
Fragments arranged by size:
4168 418 336 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1255 1507 1591 3109 1255
Size: 252 84 1518 3236
Fragments arranged by size:
3236 1518 252 84
FspI TGC'GCA
Cuts at: 2871 2871
Size: 5090
HaeII r_GCGC'y
Cuts at: 2524 2772 3794 4773 2524
Size: 248 1022 979 2841
Fragments arranged by size:
2841 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2932 2962 2964 2994 2932
Size: 30 2 30 5028
Fragments arranged by size:
5028 30 30 2
HincII GTy'rAC
Cuts at: 2129 2129
Size: 5090
KasI G'GCGC_C
Cuts at: 2520 2768 2520
Size: 248 4842
KpnI G_GTAC'C
Cuts at: 2110 2110
Size: 5090
MluI A'CGCG_T
Cuts at: 4523 4523
Size: 5090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1679 3761 3945 1679
Size: 2082 184 2824
Fragments arranged by size:
2824 2082 184
MscI TGG'CCA
Cuts at: 2851 2851
Size: 5090
MslI CAynn'nnrTG
Cuts at: 375 377 1576 3079 3206 375
Size: 2 1199 1503 127 2259
Fragments arranged by size:
2259 1503 1199 127 2
MspA1I CmG'CkG
Cuts at: 115 2201 2515 2875 3888 4133 115
Size: 2086 314 360 1013 245 1072
Fragments arranged by size:
2086 1072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3271 3271
Size: 5090
NarI GG'CG_CC
Cuts at: 2521 2769 2521
Size: 248 4842
NcoI C'CATG_G
Cuts at: 107 107
Size: 5090
NgoMIV G'CCGG_C
Cuts at: 3269 3269
Size: 5090
NsiI A_TGCA'T
Cuts at: 709 1372 709
Size: 663 4427
NspI r_CATG'y
Cuts at: 594 1034 2143 2643 3550 594
Size: 440 1109 500 907 2134
Fragments arranged by size:
2134 1109 907 500 440
PciI A'CATG_T
Cuts at: 590 3546 590
Size: 2956 2134
PfoI T'CCnGG_A
Cuts at: 803 803
Size: 5090
PmeI GTTT'AAAC
Cuts at: 2089 2089
Size: 5090
PmlI CAC'GTG
Cuts at: 463 463
Size: 5090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 148 180 4229 4261 148
Size: 32 4049 32 977
Fragments arranged by size:
4049 977 32 32
PsiI TTA'TAA
Cuts at: 1196 1196
Size: 5090
PstI C_TGCA'G
Cuts at: 1120 2137 2822 1120
Size: 1017 685 3388
Fragments arranged by size:
3388 1017 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5090
PvuII CAG'CTG
Cuts at: 2515 2875 2515
Size: 360 4730
RsrII CG'GwC_CG
Cuts at: 3285 3285
Size: 5090
SacI G_AGCT'C
Cuts at: 2034 2104 2034
Size: 70 5020
SalI G'TCGA_C
Cuts at: 2127 2127
Size: 5090
SapI GCTCTTCn'nnn_
Cuts at: 568 3113 3323 568
Size: 2545 210 2335
Fragments arranged by size:
2545 2335 210
SbfI CC_TGCA'GG
Cuts at: 2137 2137
Size: 5090
ScaI AGT'ACT
Cuts at: 4607 4607
Size: 5090
SfcI C'TryA_G
Cuts at: 33 317 1116 1137 1977 2133 2818 3811 4002
Size: 284 799 21 840 156 685 993 191
Cuts at: 4002 33
Size: 1121
Fragments arranged by size:
1121 993 840 799 685 284 191 156
21
SfoI GGC'GCC
Cuts at: 2522 2770 2522
Size: 248 4842
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
SmaI CCC'GGG
Cuts at: 2112 2112
Size: 5090
SmlI C'TyrA_G
Cuts at: 199 2245 3160 3652 3914 199
Size: 2046 915 492 262 1375
Fragments arranged by size:
2046 1375 915 492 262
SnaBI TAC'GTA
Cuts at: 1953 1953
Size: 5090
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5090
SphI G_CATG'C
Cuts at: 2143 2643 2143
Size: 500 4590
StuI AGG'CCT
Cuts at: 1051 1191 1275 1471 1527 1555 1611 1779 1051
Size: 140 84 196 56 28 56 168 4362
Fragments arranged by size:
4362 196 168 140 84 56 56 28
StyI C'CwwG_G
Cuts at: 107 747 2224 2578 107
Size: 640 1477 354 2619
Fragments arranged by size:
2619 1477 640 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 389 2673 2806 3265 3474 389
Size: 2284 133 459 209 2005
Fragments arranged by size:
2284 2005 459 209 133
TatI w'GTAC_w
Cuts at: 170 580 1665 4605 170
Size: 410 1085 2940 655
Fragments arranged by size:
2940 1085 655 410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 200 232 4607 4639 200
Size: 32 4375 32 651
Fragments arranged by size:
4375 651 32 32
Tth111I GACn'n_nGTC
Cuts at: 2887 2887
Size: 5090
XbaI T'CTAG_A
Cuts at: 2121 2121
Size: 5090
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2585 2585
Size: 5090
XmaI C'CCGG_G
Cuts at: 2110 2110
Size: 5090
XmnI GAAnn'nnTTC
Cuts at: 538 799 1051 1555 538
Size: 261 252 504 4073
Fragments arranged by size:
4073 504 261 252
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI
PmlI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI