Restriction map for
pF1KB6193
(Circular) (Six-base) MAPSORT of: pF1KB6193.seq Check: 4553 from: 1 to: 5099
pF1KB6193 5099 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1193 1700 1718 2137 1193
Size: 507 18 419 4155
Fragments arranged by size:
4155 507 419 18
Acc65I G'GTAC_C
Cuts at: 2115 2115
Size: 5099
AclI AA'CG_TT
Cuts at: 4939 4939
Size: 5099
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 500 896 940 1927 2017 2056 2924 3356
Size: 40 396 44 987 90 39 868 432
Cuts at: 3356 4103 4371 460
Size: 747 268 1188
Fragments arranged by size:
1188 987 868 747 432 396 268 90
44 40 39
AflIII A'CryG_T
Cuts at: 2451 3555 4532 2451
Size: 1104 977 3018
Fragments arranged by size:
3018 1104 977
AgeI A'CCGG_T
Cuts at: 3487 3487
Size: 5099
AlwNI CAG_nnn'CTG
Cuts at: 151 309 1848 1965 2404 3971 151
Size: 158 1539 117 439 1567 1279
Fragments arranged by size:
1567 1539 1279 439 158 117
ApaLI G'TGCA_C
Cuts at: 3869 3869
Size: 5099
ApoI r'AATT_y
Cuts at: 1388 1411 1578 2103 4452 4708 1388
Size: 23 167 525 2349 256 1779
Fragments arranged by size:
2349 1779 525 256 167 23
AseI AT'TA_AT
Cuts at: 20 536 1244 20
Size: 516 708 3875
Fragments arranged by size:
3875 708 516
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5099
AvaI C'yCGr_G
Cuts at: 348 611 817 2119 348
Size: 263 206 1302 3328
Fragments arranged by size:
3328 1302 263 206
BamHI G'GATC_C
Cuts at: 2124 2124
Size: 5099
BanI G'GyrC_C
Cuts at: 2069 2115 2529 2777 2812 2069
Size: 46 414 248 35 4356
Fragments arranged by size:
4356 414 248 46 35
BanII G_rGCy'C
Cuts at: 291 349 361 1100 2113 3143 291
Size: 58 12 739 1013 1030 2247
Fragments arranged by size:
2247 1030 1013 739 58 12
BbeI G_GCGC'C
Cuts at: 2073 2533 2781 2073
Size: 460 248 4391
Fragments arranged by size:
4391 460 248
BbsI GAAGACnn'nnnn_
Cuts at: 884 1211 1310 1634 884
Size: 327 99 324 4349
Fragments arranged by size:
4349 327 324 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 390 424 390
Size: 34 5065
BciVI GTATCCnnnnn_n'
Cuts at: 2997 3758 5099 2997
Size: 761 1341 2997
Fragments arranged by size:
2997 1341 761
BclI T'GATC_A
Cuts at: 1901 2619 1901
Size: 718 4381
BglII A'GATC_T
Cuts at: 1007 1835 2614 4651 1007
Size: 828 779 2037 1455
Fragments arranged by size:
2037 1455 828 779
BlpI GC'TnA_GC
Cuts at: 976 2211 976
Size: 1235 3864
Bme1580I G_kGCm'C
Cuts at: 2724 2817 3873 2724
Size: 93 1056 3950
Fragments arranged by size:
3950 1056 93
BmgBI CAC'GTC
Cuts at: 894 894
Size: 5099
BmrI ACTGGGnnnn_n'
Cuts at: 755 2076 2311 2392 2727 4871 755
Size: 1321 235 81 335 2144 983
Fragments arranged by size:
2144 1321 983 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 624 3466 624
Size: 2842 2257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1066 1869 1906 619
Size: 447 803 37 3812
Fragments arranged by size:
3812 803 447 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 3154 3646 3944 2275
Size: 879 492 298 3430
Fragments arranged by size:
3430 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4803 4803
Size: 5099
BsaAI yAC'GTr
Cuts at: 2452 2452
Size: 5099
BsaBI GATnn'nnATC
Cuts at: 6 2613 4650 6
Size: 2607 2037 455
Fragments arranged by size:
2607 2037 455
BsaHI Gr'CG_yC
Cuts at: 2070 2530 2778 3480 4976 2070
Size: 460 248 702 1496 2193
Fragments arranged by size:
2193 1496 702 460 248
BsaWI w'CCGG_w
Cuts at: 1200 2288 2809 3487 3761 3908 4319 1200
Size: 1088 521 678 274 147 411 1980
Fragments arranged by size:
1980 1088 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 361
Size: 30 5069
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 1186 1846 259
Size: 927 660 3512
Fragments arranged by size:
3512 927 660
BseYI C'CCAG_C
Cuts at: 2524 3282 3859 4355 2524
Size: 758 577 496 3268
Fragments arranged by size:
3268 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 252
Size: 5099
BsiEI CG_ry'CG
Cuts at: 104 2641 2687 3895 104
Size: 2537 46 1208 1308
Fragments arranged by size:
2537 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 349 361 1100 2113 2891 3081 3873 349
Size: 12 739 1013 778 190 792 1575
Fragments arranged by size:
1575 1013 792 778 739 190 12
BsiWI C'GTAC_G
Cuts at: 1928 1928
Size: 5099
BsmI GAATG_Cn'
Cuts at: 555 555
Size: 5099
Bsp1286I G_dGCh'C
Cuts at: 291 349 361 1100 2113 2724 2817 2891 3081
Size: 58 12 739 1013 611 93 74 190
Cuts at: 3081 3143 3873 291
Size: 62 730 1517
Fragments arranged by size:
1517 1013 739 730 611 190 93 74
62 58 12
BspEI T'CCGG_A
Cuts at: 4319 4319
Size: 5099
BspHI T'CATG_A
Cuts at: 582 4275 582
Size: 3693 1406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2149 2363 2665 3046 2149
Size: 214 302 381 4202
Fragments arranged by size:
4202 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2004 2043 3391 3445 4929 5094 50
Size: 1954 39 1348 54 1484 165 55
Fragments arranged by size:
1954 1484 1348 165 55 54 39
BsrDI GCAATG_nn'
Cuts at: 773 1729 3011 773
Size: 956 1282 2861
Fragments arranged by size:
2861 1282 956
BsrFI r'CCGG_y
Cuts at: 1509 2072 3097 3278 3487 1509
Size: 563 1025 181 209 3121
Fragments arranged by size:
3121 1025 563 209 181
BssSI C'ACGA_G
Cuts at: 907 1333 3370 3728 907
Size: 426 2037 358 2278
Fragments arranged by size:
2278 2037 426 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1965 2476 1965
Size: 511 4588
BstBI TT'CG_AA
Cuts at: 3460 3460
Size: 5099
BstXI CCAn_nnnn'nTGG
Cuts at: 768 768
Size: 5099
BstYI r'GATC_y
Cuts at: 1 624 962 1007 1038 1835 2124 2591 2614
Size: 623 338 45 31 797 289 467 23
Cuts at: 2614 2949 3195 4207 4293 4305 4651 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 797 623 467 449 346 338 335
289 246 86 45 31 23 12
BstZ17I GTA'TAC
Cuts at: 1719 1719
Size: 5099
BtgI C'CryG_G
Cuts at: 107 1061 1826 107
Size: 954 765 3380
Fragments arranged by size:
3380 954 765
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 114 309 540 3049 3230 3393 114
Size: 195 231 2509 181 163 1820
Fragments arranged by size:
2509 1820 231 195 181 163
BtsI GCAGTG_nn'
Cuts at: 1265 2342 1265
Size: 1077 4022
DraI TTT'AAA
Cuts at: 2098 2098
Size: 5099
DraIII CAC_nnn'GTG
Cuts at: 1533 1533
Size: 5099
DrdI GACnn_nn'nnGTC
Cuts at: 2805 3663 2805
Size: 858 4241
EaeI y'GGCC_r
Cuts at: 1932 2684 2858 3249 3276 4621 1932
Size: 752 174 391 27 1345 2410
Fragments arranged by size:
2410 1345 752 391 174 27
EagI C'GGCC_G
Cuts at: 2684 2684
Size: 5099
EarI CTCTTCn'nnn_
Cuts at: 1499 1580 1827 3122 3332 1499
Size: 81 247 1295 210 3266
Fragments arranged by size:
3266 1295 247 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 3307 3617 3763 4909 1660
Size: 1647 310 146 1146 1850
Fragments arranged by size:
1850 1647 1146 310 146
EcoICRI GAG'CTC
Cuts at: 347 359 1098 2111 347
Size: 12 739 1013 3335
Fragments arranged by size:
3335 1013 739 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 500 619 896 940 1066 1869 1906 1927
Size: 40 119 277 44 126 803 37 21
Cuts at: 1927 2017 2056 2924 3356 4103 4371 460
Size: 90 39 868 432 747 268 1188
Fragments arranged by size:
1188 868 803 747 432 277 268 126
119 90 44 40 39 37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 922 922
Size: 5099
EcoO109I rG'GnC_Cy
Cuts at: 306 2238 306
Size: 1932 3167
EcoRI G'AATT_C
Cuts at: 2103 2103
Size: 5099
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1029 3118 1029
Size: 2089 3010
FspI TGC'GCA
Cuts at: 2880 2880
Size: 5099
HaeII r_GCGC'y
Cuts at: 2073 2533 2781 3803 4782 2073
Size: 460 248 1022 979 2390
Fragments arranged by size:
2390 1022 979 460 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 361 363 393 461 493 992 1024 1842
Size: 30 2 30 68 32 499 32 818
Cuts at: 1842 1874 2049 2081 2941 2971 2973 3003 331
Size: 32 175 32 860 30 2 30 2427
Fragments arranged by size:
2427 860 818 499 175 68 32 32
32 32 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 1194 2138 1194
Size: 944 4155
KasI G'GCGC_C
Cuts at: 2069 2529 2777 2069
Size: 460 248 4391
Fragments arranged by size:
4391 460 248
KpnI G_GTAC'C
Cuts at: 2119 2119
Size: 5099
MluI A'CGCG_T
Cuts at: 4532 4532
Size: 5099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 1353 3770 3954 347
Size: 1006 2417 184 1492
Fragments arranged by size:
2417 1492 1006 184
MscI TGG'CCA
Cuts at: 2860 2860
Size: 5099
MslI CAynn'nnrTG
Cuts at: 3088 3215 3088
Size: 127 4972
MspA1I CmG'CkG
Cuts at: 148 404 426 975 1988 2210 2524 2884 3897
Size: 256 22 549 1013 222 314 360 1013
Cuts at: 3897 4142 148
Size: 245 1105
Fragments arranged by size:
1105 1013 1013 549 360 314 256 245
222 22
NaeI GCC'GGC
Cuts at: 1511 2074 3280 1511
Size: 563 1206 3330
Fragments arranged by size:
3330 1206 563
NarI GG'CG_CC
Cuts at: 2070 2530 2778 2070
Size: 460 248 4391
Fragments arranged by size:
4391 460 248
NcoI C'CATG_G
Cuts at: 107 1826 107
Size: 1719 3380
NgoMIV G'CCGG_C
Cuts at: 1509 2072 3278 1509
Size: 563 1206 3330
Fragments arranged by size:
3330 1206 563
NspI r_CATG'y
Cuts at: 2152 2652 3559 2152
Size: 500 907 3692
Fragments arranged by size:
3692 907 500
PciI A'CATG_T
Cuts at: 3555 3555
Size: 5099
PflMI CCAn_nnn'nTGG
Cuts at: 810 810
Size: 5099
PfoI T'CCnGG_A
Cuts at: 620 965 620
Size: 345 4754
PmeI GTTT'AAAC
Cuts at: 2098 2098
Size: 5099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 4238
Size: 32 5067
PpuMI rG'GwC_Cy
Cuts at: 306 306
Size: 5099
PstI C_TGCA'G
Cuts at: 998 1961 1970 2146 2831 998
Size: 963 9 176 685 3266
Fragments arranged by size:
3266 963 685 176 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5099
PvuII CAG'CTG
Cuts at: 148 975 1988 2524 2884 148
Size: 827 1013 536 360 2363
Fragments arranged by size:
2363 1013 827 536 360
RsrII CG'GwC_CG
Cuts at: 3294 3294
Size: 5099
SacI G_AGCT'C
Cuts at: 349 361 1100 2113 349
Size: 12 739 1013 3335
Fragments arranged by size:
3335 1013 739 12
SalI G'TCGA_C
Cuts at: 1192 2136 1192
Size: 944 4155
SapI GCTCTTCn'nnn_
Cuts at: 1499 3122 3332 1499
Size: 1623 210 3266
Fragments arranged by size:
3266 1623 210
SbfI CC_TGCA'GG
Cuts at: 1970 2146 1970
Size: 176 4923
ScaI AGT'ACT
Cuts at: 4616 4616
Size: 5099
SexAI A'CCwGG_T
Cuts at: 1712 1712
Size: 5099
SfcI C'TryA_G
Cuts at: 33 994 1292 1957 1966 1983 2142 2827 3820
Size: 961 298 665 9 17 159 685 993
Cuts at: 3820 4011 33
Size: 191 1121
Fragments arranged by size:
1121 993 961 685 665 298 191 159
17 9
SfoI GGC'GCC
Cuts at: 2071 2531 2779 2071
Size: 460 248 4391
Fragments arranged by size:
4391 460 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5099
SmaI CCC'GGG
Cuts at: 2121 2121
Size: 5099
SmlI C'TyrA_G
Cuts at: 2254 3169 3661 3923 2254
Size: 915 492 262 3430
Fragments arranged by size:
3430 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5099
SphI G_CATG'C
Cuts at: 2152 2652 2152
Size: 500 4599
StyI C'CwwG_G
Cuts at: 107 1826 2233 2587 107
Size: 1719 407 354 2619
Fragments arranged by size:
2619 1719 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1040 2682 2815 3274 3483 1040
Size: 1642 133 459 209 2656
Fragments arranged by size:
2656 1642 459 209 133
TatI w'GTAC_w
Cuts at: 719 1351 4614 719
Size: 632 3263 1204
Fragments arranged by size:
3263 1204 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4616 4648 4616
Size: 32 5067
Tth111I GACn'n_nGTC
Cuts at: 2896 2896
Size: 5099
XbaI T'CTAG_A
Cuts at: 2130 2130
Size: 5099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 663 2594 423
Size: 240 1931 2928
Fragments arranged by size:
2928 1931 240
XmaI C'CCGG_G
Cuts at: 2119 2119
Size: 5099
XmnI GAAnn'nnTTC
Cuts at: 1009 1307 1009
Size: 298 4801
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
Bpu10I BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI