(Circular) (Six-base) MAPSORT of: pF1KB6195.seq Check: 5807 from: 1 to: 5102 pF1KB6195 5102 bp With 182 enzymes: SgfI * October 7, 2009 14:19 .. AccI GT'mk_AC Cuts at: 2140 2140 Size: 5102 Acc65I G'GTAC_C Cuts at: 225 2118 225 Size: 1893 3209 AclI AA'CG_TT Cuts at: 4942 4942 Size: 5102 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 432 1286 1814 1819 2927 3359 4106 4374 432 Size: 854 528 5 1108 432 747 268 1160 Fragments arranged by size: 1160 1108 854 747 528 432 268 5 AflIII A'CryG_T Cuts at: 2454 3558 4535 2454 Size: 1104 977 3021 Fragments arranged by size: 3021 1104 977 AgeI A'CCGG_T Cuts at: 3490 3490 Size: 5102 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 361 361 Size: 5102 AlwNI CAG_nnn'CTG Cuts at: 330 963 1627 1945 2407 3974 330 Size: 633 664 318 462 1567 1458 Fragments arranged by size: 1567 1458 664 633 462 318 ApaI G_GGCC'C Cuts at: 1643 1770 1643 Size: 127 4975 ApaLI G'TGCA_C Cuts at: 3872 3872 Size: 5102 ApoI r'AATT_y Cuts at: 1163 2106 4455 4711 1163 Size: 943 2349 256 1554 Fragments arranged by size: 2349 1554 943 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5102 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5102 AvaI C'yCGr_G Cuts at: 255 2122 255 Size: 1867 3235 AvrII C'CTAG_G Cuts at: 1323 1708 1323 Size: 385 4717 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 648 681 648 Size: 33 5069 BamHI G'GATC_C Cuts at: 2127 2127 Size: 5102 BanI G'GyrC_C Cuts at: 225 1605 2118 2532 2780 2815 225 Size: 1380 513 414 248 35 2512 Fragments arranged by size: 2512 1380 513 414 248 35 BanII G_rGCy'C Cuts at: 530 1351 1545 1643 1770 1814 1958 2087 2116 Size: 821 194 98 127 44 144 129 29 Cuts at: 2116 3146 530 Size: 1030 2486 Fragments arranged by size: 2486 1030 821 194 144 129 127 98 44 29 BbeI G_GCGC'C Cuts at: 1609 2536 2784 1609 Size: 927 248 3927 Fragments arranged by size: 3927 927 248 BbsI GAAGACnn'nnnn_ Cuts at: 194 622 652 194 Size: 428 30 4644 Fragments arranged by size: 4644 428 30 BciVI GTATCCnnnnn_n' Cuts at: 3000 3761 5102 3000 Size: 761 1341 3000 Fragments arranged by size: 3000 1341 761 BclI T'GATC_A Cuts at: 2622 2622 Size: 5102 BglI GCCn_nnn'nGGC Cuts at: 1060 1069 1540 1889 1060 Size: 9 471 349 4273 Fragments arranged by size: 4273 471 349 9 BglII A'GATC_T Cuts at: 443 1117 2617 4654 443 Size: 674 1500 2037 891 Fragments arranged by size: 2037 1500 891 674 BlpI GC'TnA_GC Cuts at: 1988 2214 1988 Size: 226 4876 Bme1580I G_kGCm'C Cuts at: 912 1310 1643 1770 2727 2820 3876 912 Size: 398 333 127 957 93 1056 2138 Fragments arranged by size: 2138 1056 957 398 333 127 93 BmrI ACTGGGnnnn_n' Cuts at: 122 629 1933 2314 2395 2730 4874 122 Size: 507 1304 381 81 335 2144 350 Fragments arranged by size: 2144 1304 507 381 350 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 387 609 1977 3469 387 Size: 222 1368 1492 2020 Fragments arranged by size: 2020 1492 1368 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 283 579 601 1086 1165 1241 1423 283 Size: 296 22 485 79 76 182 3962 Fragments arranged by size: 3962 485 296 182 79 76 22 Bpu10I CC'TnA_GC Cuts at: 1968 1968 Size: 5102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1972 2278 3157 3649 3947 1972 Size: 306 879 492 298 3127 Fragments arranged by size: 3127 879 492 306 298 BsaI GGTCTCn'nnnn_ Cuts at: 1113 1183 1792 4806 1113 Size: 70 609 3014 1409 Fragments arranged by size: 3014 1409 609 70 BsaAI yAC'GTr Cuts at: 2455 2455 Size: 5102 BsaBI GATnn'nnATC Cuts at: 6 2616 4653 6 Size: 2610 2037 455 Fragments arranged by size: 2610 2037 455 BsaHI Gr'CG_yC Cuts at: 1606 2533 2781 3483 4979 1606 Size: 927 248 702 1496 1729 Fragments arranged by size: 1729 1496 927 702 248 BsaWI w'CCGG_w Cuts at: 641 2291 2812 3490 3764 3911 4322 641 Size: 1650 521 678 274 147 411 1421 Fragments arranged by size: 1650 1421 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1404 1434 1890 1920 1404 Size: 30 456 30 4586 Fragments arranged by size: 4586 456 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 598 1237 1728 1898 1950 598 Size: 639 491 170 52 3750 Fragments arranged by size: 3750 639 491 170 52 BseYI C'CCAG_C Cuts at: 204 418 685 803 1309 1579 1642 1912 2037 Size: 214 267 118 506 270 63 270 125 Cuts at: 2037 2527 3285 3862 4358 204 Size: 490 758 577 496 948 Fragments arranged by size: 948 758 577 506 496 490 270 270 267 214 125 118 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 467 467 Size: 5102 BsiEI CG_ry'CG Cuts at: 104 2644 2690 3898 104 Size: 2540 46 1208 1308 Fragments arranged by size: 2540 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1814 1958 2116 2894 3084 3876 1814 Size: 144 158 778 190 792 3040 Fragments arranged by size: 3040 792 778 190 158 144 BsmI GAATG_Cn' Cuts at: 573 573 Size: 5102 BsmBI CGTCTCn'nnnn_ Cuts at: 1257 1257 Size: 5102 Bsp1286I G_dGCh'C Cuts at: 530 912 1310 1351 1545 1643 1770 1814 1958 Size: 382 398 41 194 98 127 44 144 Cuts at: 1958 2087 2116 2727 2820 2894 3084 3146 3876 Size: 129 29 611 93 74 190 62 730 Cuts at: 3876 530 Size: 1756 Fragments arranged by size: 1756 730 611 398 382 194 190 144 129 127 98 93 74 62 44 41 29 BspEI T'CCGG_A Cuts at: 641 4322 641 Size: 3681 1421 BspHI T'CATG_A Cuts at: 4278 4278 Size: 5102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 213 2152 2366 2668 3049 213 Size: 1939 214 302 381 2266 Fragments arranged by size: 2266 1939 381 302 214 BsrBI CCG'CTC Cuts at: 50 3394 3448 4932 5097 50 Size: 3344 54 1484 165 55 Fragments arranged by size: 3344 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1667 3014 1667 Size: 1347 3755 BsrFI r'CCGG_y Cuts at: 3100 3281 3490 3100 Size: 181 209 4712 Fragments arranged by size: 4712 209 181 BssSI C'ACGA_G Cuts at: 3373 3731 3373 Size: 358 4744 BstAPI GCAn_nnn'nTGC Cuts at: 2479 2479 Size: 5102 BstBI TT'CG_AA Cuts at: 3463 3463 Size: 5102 BstXI CCAn_nnnn'nTGG Cuts at: 996 996 Size: 5102 BstYI r'GATC_y Cuts at: 1 326 443 1117 1226 2026 2127 2594 2617 Size: 325 117 674 109 800 101 467 23 Cuts at: 2617 2952 3198 4210 4296 4308 4654 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 800 674 467 449 346 335 325 246 117 109 101 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1049 1804 1962 1049 Size: 755 158 4189 Fragments arranged by size: 4189 755 158 BtgI C'CryG_G Cuts at: 107 389 1058 1337 1382 1613 1772 107 Size: 282 669 279 45 231 159 3437 Fragments arranged by size: 3437 669 282 279 231 159 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3052 3233 3396 3052 Size: 181 163 4758 Fragments arranged by size: 4758 181 163 BtsI GCAGTG_nn' Cuts at: 2345 2345 Size: 5102 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1835 1870 1835 Size: 35 5067 DraI TTT'AAA Cuts at: 2101 2101 Size: 5102 DrdI GACnn_nn'nnGTC Cuts at: 1000 2808 3666 1000 Size: 1808 858 2436 Fragments arranged by size: 2436 1808 858 EaeI y'GGCC_r Cuts at: 941 2687 2861 3252 3279 4624 941 Size: 1746 174 391 27 1345 1419 Fragments arranged by size: 1746 1419 1345 391 174 27 EagI C'GGCC_G Cuts at: 2687 2687 Size: 5102 EarI CTCTTCn'nnn_ Cuts at: 628 764 1149 1790 3125 3335 628 Size: 136 385 641 1335 210 2395 Fragments arranged by size: 2395 1335 641 385 210 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3310 3620 3766 4912 3310 Size: 310 146 1146 3500 Fragments arranged by size: 3500 1146 310 146 EcoICRI GAG'CTC Cuts at: 1812 1956 2114 1812 Size: 144 158 4800 Fragments arranged by size: 4800 158 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 283 432 579 601 1086 1165 1241 1286 1423 Size: 149 147 22 485 79 76 45 137 Cuts at: 1423 1814 1819 2927 3359 4106 4374 283 Size: 391 5 1108 432 747 268 1011 Fragments arranged by size: 1108 1011 747 485 432 391 268 149 147 137 79 76 45 22 5 EcoNI CCTnn'n_nnAGG Cuts at: 1680 1680 Size: 5102 EcoO109I rG'GnC_Cy Cuts at: 302 993 1639 1733 1766 1767 1973 2241 302 Size: 691 646 94 33 1 206 268 3163 Fragments arranged by size: 3163 691 646 268 206 94 33 1 EcoRI G'AATT_C Cuts at: 2106 2106 Size: 5102 EcoRV GAT'ATC Cuts at: 174 426 174 Size: 252 4850 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 462 1491 3121 462 Size: 1029 1630 2443 Fragments arranged by size: 2443 1630 1029 FspI TGC'GCA Cuts at: 2883 2883 Size: 5102 HaeII r_GCGC'y Cuts at: 1609 2536 2784 3806 4785 1609 Size: 927 248 1022 979 1926 Fragments arranged by size: 1926 1022 979 927 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 306 338 1890 1922 2944 2974 2976 3006 306 Size: 32 1552 32 1022 30 2 30 2402 Fragments arranged by size: 2402 1552 1022 32 32 30 30 2 HincII GTy'rAC Cuts at: 386 2141 386 Size: 1755 3347 KasI G'GCGC_C Cuts at: 1605 2532 2780 1605 Size: 927 248 3927 Fragments arranged by size: 3927 927 248 KpnI G_GTAC'C Cuts at: 229 2122 229 Size: 1893 3209 MluI A'CGCG_T Cuts at: 4535 4535 Size: 5102 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 248 1618 1870 3773 3957 248 Size: 1370 252 1903 184 1393 Fragments arranged by size: 1903 1393 1370 252 184 MscI TGG'CCA Cuts at: 943 2863 943 Size: 1920 3182 MslI CAynn'nnrTG Cuts at: 2077 3091 3218 2077 Size: 1014 127 3961 Fragments arranged by size: 3961 1014 127 MspA1I CmG'CkG Cuts at: 183 208 391 490 1453 2213 2527 2887 3900 Size: 25 183 99 963 760 314 360 1013 Cuts at: 3900 4145 183 Size: 245 1140 Fragments arranged by size: 1140 1013 963 760 360 314 245 183 99 25 NaeI GCC'GGC Cuts at: 3283 3283 Size: 5102 NarI GG'CG_CC Cuts at: 1606 2533 2781 1606 Size: 927 248 3927 Fragments arranged by size: 3927 927 248 NcoI C'CATG_G Cuts at: 107 1772 107 Size: 1665 3437 NgoMIV G'CCGG_C Cuts at: 3281 3281 Size: 5102 NspI r_CATG'y Cuts at: 1174 2155 2655 3562 1174 Size: 981 500 907 2714 Fragments arranged by size: 2714 981 907 500 PasI CC'CwG_GG Cuts at: 1128 1537 1657 1128 Size: 409 120 4573 Fragments arranged by size: 4573 409 120 PciI A'CATG_T Cuts at: 3558 3558 Size: 5102 PflMI CCAn_nnn'nTGG Cuts at: 183 857 1575 183 Size: 674 718 3710 Fragments arranged by size: 3710 718 674 PfoI T'CCnGG_A Cuts at: 329 428 329 Size: 99 5003 PmeI GTTT'AAAC Cuts at: 2101 2101 Size: 5102 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1940 1972 4241 4273 1940 Size: 32 2269 32 2769 Fragments arranged by size: 2769 2269 32 32 PpuMI rG'GwC_Cy Cuts at: 302 993 1733 1973 302 Size: 691 740 240 3431 Fragments arranged by size: 3431 740 691 240 PspOMI G'GGCC_C Cuts at: 1639 1766 1639 Size: 127 4975 PstI C_TGCA'G Cuts at: 1367 2149 2834 1367 Size: 782 685 3635 Fragments arranged by size: 3635 782 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5102 PvuII CAG'CTG Cuts at: 183 208 490 2527 2887 183 Size: 25 282 2037 360 2398 Fragments arranged by size: 2398 2037 360 282 25 RsrII CG'GwC_CG Cuts at: 3297 3297 Size: 5102 SacI G_AGCT'C Cuts at: 1814 1958 2116 1814 Size: 144 158 4800 Fragments arranged by size: 4800 158 144 SacII CC_GC'GG Cuts at: 392 392 Size: 5102 SalI G'TCGA_C Cuts at: 2139 2139 Size: 5102 SanDI GG'GwC_CC Cuts at: 1973 1973 Size: 5102 SapI GCTCTTCn'nnn_ Cuts at: 764 1149 3125 3335 764 Size: 385 1976 210 2531 Fragments arranged by size: 2531 1976 385 210 SbfI CC_TGCA'GG Cuts at: 2149 2149 Size: 5102 ScaI AGT'ACT Cuts at: 1413 4619 1413 Size: 3206 1896 SexAI A'CCwGG_T Cuts at: 995 995 Size: 5102 SfcI C'TryA_G Cuts at: 33 121 1363 1697 2145 2830 3823 4014 33 Size: 88 1242 334 448 685 993 191 1121 Fragments arranged by size: 1242 1121 993 685 448 334 191 88 SfiI GGCCn_nnn'nGGCC Cuts at: 1060 1069 1060 Size: 9 5093 SfoI GGC'GCC Cuts at: 1607 2534 2782 1607 Size: 927 248 3927 Fragments arranged by size: 3927 927 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5102 SmaI CCC'GGG Cuts at: 2124 2124 Size: 5102 SmlI C'TyrA_G Cuts at: 1951 2257 3172 3664 3926 1951 Size: 306 915 492 262 3127 Fragments arranged by size: 3127 915 492 306 262 SpeI A'CTAG_T Cuts at: 67 556 67 Size: 489 4613 SphI G_CATG'C Cuts at: 2155 2655 2155 Size: 500 4602 StuI AGG'CCT Cuts at: 592 1054 1489 592 Size: 462 435 4205 Fragments arranged by size: 4205 462 435 StyI C'CwwG_G Cuts at: 107 650 989 1323 1708 1772 2236 2590 107 Size: 543 339 334 385 64 464 354 2619 Fragments arranged by size: 2619 543 464 385 354 339 334 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1165 1626 2685 2818 3277 3486 1165 Size: 461 1059 133 459 209 2781 Fragments arranged by size: 2781 1059 461 459 209 133 TatI w'GTAC_w Cuts at: 251 788 1411 4617 251 Size: 537 623 3206 736 Fragments arranged by size: 3206 736 623 537 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4619 4651 4619 Size: 32 5070 Tth111I GACn'n_nGTC Cuts at: 1902 2899 1902 Size: 997 4105 XbaI T'CTAG_A Cuts at: 2133 2133 Size: 5102 XcmI CCAnnnn_n'nnnnTGG Cuts at: 426 996 1065 1576 2597 426 Size: 570 69 511 1021 2931 Fragments arranged by size: 2931 1021 570 511 69 XmaI C'CCGG_G Cuts at: 2122 2122 Size: 5102 XmnI GAAnn'nnTTC Cuts at: 464 464 Size: 5102 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI