Restriction map for
pF1KB6202
(Circular) (Six-base) MAPSORT of: pF1KB6202.seq Check: 7735 from: 1 to: 5246
pF1KB6202 5246 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 2284 2284
Size: 5246
Acc65I G'GTAC_C
Cuts at: 2262 2262
Size: 5246
AclI AA'CG_TT
Cuts at: 5086 5086
Size: 5246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 321 398 1227 3071 3503 4250 4518 198
Size: 123 77 829 1844 432 747 268 926
Fragments arranged by size:
1844 926 829 747 432 268 123 77
AflIII A'CryG_T
Cuts at: 2598 3702 4679 2598
Size: 1104 977 3165
Fragments arranged by size:
3165 1104 977
AgeI A'CCGG_T
Cuts at: 3634 3634
Size: 5246
AleI CACnn'nnGTG
Cuts at: 1544 1853 1544
Size: 309 4937
AlwNI CAG_nnn'CTG
Cuts at: 147 237 1628 2551 4118 147
Size: 90 1391 923 1567 1275
Fragments arranged by size:
1567 1391 1275 923 90
ApaLI G'TGCA_C
Cuts at: 1872 4016 1872
Size: 2144 3102
ApoI r'AATT_y
Cuts at: 123 563 920 1004 1340 1592 1676 1760 1844
Size: 440 357 84 336 252 84 84 84
Cuts at: 1844 1908 2096 2250 4599 4855 123
Size: 64 188 154 2349 256 514
Fragments arranged by size:
2349 514 440 357 336 256 252 188
154 84 84 84 84 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 5246
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
AvaI C'yCGr_G
Cuts at: 2266 2266
Size: 5246
BamHI G'GATC_C
Cuts at: 2271 2271
Size: 5246
BanI G'GyrC_C
Cuts at: 2262 2676 2924 2959 2262
Size: 414 248 35 4549
Fragments arranged by size:
4549 414 248 35
BanII G_rGCy'C
Cuts at: 284 2260 3290 284
Size: 1976 1030 2240
Fragments arranged by size:
2240 1976 1030
BbeI G_GCGC'C
Cuts at: 2680 2928 2680
Size: 248 4998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5212
BciVI GTATCCnnnnn_n'
Cuts at: 3144 3905 5246 3144
Size: 761 1341 3144
Fragments arranged by size:
3144 1341 761
BclI T'GATC_A
Cuts at: 2766 2766
Size: 5246
BfrBI ATG'CAT
Cuts at: 1366 1752 1366
Size: 386 4860
BglII A'GATC_T
Cuts at: 2761 4798 2761
Size: 2037 3209
BlpI GC'TnA_GC
Cuts at: 2358 2358
Size: 5246
Bme1580I G_kGCm'C
Cuts at: 1876 2871 2964 4020 1876
Size: 995 93 1056 3102
Fragments arranged by size:
3102 1056 995 93
BmgBI CAC'GTC
Cuts at: 201 201
Size: 5246
BmrI ACTGGGnnnn_n'
Cuts at: 2458 2539 2874 5018 2458
Size: 81 335 2144 2686
Fragments arranged by size:
2686 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1291 3613 1291
Size: 2322 2924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1706 298
Size: 1408 3838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 2065 2422 3301 3793 4091 892
Size: 1173 357 879 492 298 2047
Fragments arranged by size:
2047 1173 879 492 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 4950 4950
Size: 5246
BsaAI yAC'GTr
Cuts at: 2599 2599
Size: 5246
BsaBI GATnn'nnATC
Cuts at: 6 1583 2760 4797 6
Size: 1577 1177 2037 455
Fragments arranged by size:
2037 1577 1177 455
BsaHI Gr'CG_yC
Cuts at: 2677 2925 3627 5123 2677
Size: 248 702 1496 2800
Fragments arranged by size:
2800 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1768 2435 2956 3634 3908 4055 4466 1768
Size: 667 521 678 274 147 411 2548
Fragments arranged by size:
2548 678 667 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 385 238
Size: 147 5099
BseYI C'CCAG_C
Cuts at: 2671 3429 4006 4502 2671
Size: 758 577 496 3415
Fragments arranged by size:
3415 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2788 2834 4042 104
Size: 2684 46 1208 1308
Fragments arranged by size:
2684 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 284 1876 2260 3038 3228 4020 284
Size: 1592 384 778 190 792 1510
Fragments arranged by size:
1592 1510 792 778 384 190
BsmI GAATG_Cn'
Cuts at: 347 1288 1465 1624 1717 2044 347
Size: 941 177 159 93 327 3549
Fragments arranged by size:
3549 941 327 177 159 93
Bsp1286I G_dGCh'C
Cuts at: 284 1876 2260 2871 2964 3038 3228 3290 4020
Size: 1592 384 611 93 74 190 62 730
Cuts at: 4020 284
Size: 1510
Fragments arranged by size:
1592 1510 730 611 384 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4466 4466
Size: 5246
BspHI T'CATG_A
Cuts at: 4422 4422
Size: 5246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1892 2296 2510 2812 3193 1892
Size: 404 214 302 381 3945
Fragments arranged by size:
3945 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3538 3592 5076 5241 50
Size: 3488 54 1484 165 55
Fragments arranged by size:
3488 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 765 3158 765
Size: 2393 2853
BsrFI r'CCGG_y
Cuts at: 3244 3425 3634 3244
Size: 181 209 4856
Fragments arranged by size:
4856 209 181
BssSI C'ACGA_G
Cuts at: 657 3517 3875 657
Size: 2860 358 2028
Fragments arranged by size:
2860 2028 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1712 2623 1712
Size: 911 4335
BstBI TT'CG_AA
Cuts at: 3607 3607
Size: 5246
BstEII G'GTnAC_C
Cuts at: 868 868
Size: 5246
BstXI CCAn_nnnn'nTGG
Cuts at: 1033 1210 1546 1033
Size: 177 336 4733
Fragments arranged by size:
4733 336 177
BstYI r'GATC_y
Cuts at: 1 169 2271 2738 2761 3096 3342 4354 4440
Size: 168 2102 467 23 335 246 1012 86
Cuts at: 4440 4452 4798 1
Size: 12 346 449
Fragments arranged by size:
2102 1012 467 449 346 335 246 168
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 140 140
Size: 5246
BtgI C'CryG_G
Cuts at: 206 206
Size: 5246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3196 3377 3540 3196
Size: 181 163 4902
Fragments arranged by size:
4902 181 163
BtsI GCAGTG_nn'
Cuts at: 308 1293 1377 1629 1713 1797 2489 308
Size: 985 84 252 84 84 692 3065
Fragments arranged by size:
3065 985 692 252 84 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1107 1142 1107
Size: 35 5211
DraI TTT'AAA
Cuts at: 2245 2245
Size: 5246
DrdI GACnn_nn'nnGTC
Cuts at: 2952 3810 2952
Size: 858 4388
EaeI y'GGCC_r
Cuts at: 891 2831 3005 3396 3423 4768 891
Size: 1940 174 391 27 1345 1369
Fragments arranged by size:
1940 1369 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2831 2831
Size: 5246
EarI CTCTTCn'nnn_
Cuts at: 301 822 1246 3269 3479 301
Size: 521 424 2023 210 2068
Fragments arranged by size:
2068 2023 521 424 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3454 3764 3910 5056 3454
Size: 310 146 1146 3644
Fragments arranged by size:
3644 1146 310 146
EcoICRI GAG'CTC
Cuts at: 282 2258 282
Size: 1976 3270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 298 321 398 1227 1706 3071 3503 4250
Size: 100 23 77 829 479 1365 432 747
Cuts at: 4250 4518 198
Size: 268 926
Fragments arranged by size:
1365 926 829 747 479 432 268 100
77 23
EcoO109I rG'GnC_Cy
Cuts at: 144 636 662 2385 144
Size: 492 26 1723 3005
Fragments arranged by size:
3005 1723 492 26
EcoRI G'AATT_C
Cuts at: 1004 1592 1760 1844 2096 2250 1004
Size: 588 168 84 252 154 4000
Fragments arranged by size:
4000 588 252 168 154 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 5246
FspI TGC'GCA
Cuts at: 3027 3027
Size: 5246
HaeII r_GCGC'y
Cuts at: 2680 2928 3950 4929 2680
Size: 248 1022 979 2997
Fragments arranged by size:
2997 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1221 1253 1485 1517 1989 2021 3088 3118 3120
Size: 32 232 32 472 32 1067 30 2
Cuts at: 3120 3150 1221
Size: 30 3317
Fragments arranged by size:
3317 1067 472 232 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1403 2285 1403
Size: 882 4364
HindIII A'AGCT_T
Cuts at: 449 449
Size: 5246
KasI G'GCGC_C
Cuts at: 2676 2924 2676
Size: 248 4998
KpnI G_GTAC'C
Cuts at: 2266 2266
Size: 5246
MfeI C'AATT_G
Cuts at: 621 621
Size: 5246
MluI A'CGCG_T
Cuts at: 4679 4679
Size: 5246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3917 4101 3917
Size: 184 5062
MscI TGG'CCA
Cuts at: 893 3007 893
Size: 2114 3132
MslI CAynn'nnrTG
Cuts at: 1031 1097 1208 1349 1544 1853 3235 3362 1031
Size: 66 111 141 195 309 1382 127 2915
Fragments arranged by size:
2915 1382 309 195 141 127 111 66
MspA1I CmG'CkG
Cuts at: 2357 2671 3031 4044 4289 2357
Size: 314 360 1013 245 3314
Fragments arranged by size:
3314 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3427 3427
Size: 5246
NarI GG'CG_CC
Cuts at: 2677 2925 2677
Size: 248 4998
NgoMIV G'CCGG_C
Cuts at: 3425 3425
Size: 5246
NsiI A_TGCA'T
Cuts at: 1368 1754 1368
Size: 386 4860
NspI r_CATG'y
Cuts at: 1370 1752 2299 2799 3706 1370
Size: 382 547 500 907 2910
Fragments arranged by size:
2910 907 547 500 382
PasI CC'CwG_GG
Cuts at: 640 640
Size: 5246
PciI A'CATG_T
Cuts at: 3702 3702
Size: 5246
PmeI GTTT'AAAC
Cuts at: 2245 2245
Size: 5246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 4385
Size: 32 5214
PpuMI rG'GwC_Cy
Cuts at: 144 636 144
Size: 492 4754
PstI C_TGCA'G
Cuts at: 832 2293 2978 832
Size: 1461 685 3100
Fragments arranged by size:
3100 1461 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5246
PvuII CAG'CTG
Cuts at: 2671 3031 2671
Size: 360 4886
RsrII CG'GwC_CG
Cuts at: 3441 3441
Size: 5246
SacI G_AGCT'C
Cuts at: 284 2260 284
Size: 1976 3270
SalI G'TCGA_C
Cuts at: 2283 2283
Size: 5246
SapI GCTCTTCn'nnn_
Cuts at: 822 3269 3479 822
Size: 2447 210 2589
Fragments arranged by size:
2589 2447 210
SbfI CC_TGCA'GG
Cuts at: 2293 2293
Size: 5246
ScaI AGT'ACT
Cuts at: 4763 4763
Size: 5246
SfcI C'TryA_G
Cuts at: 33 285 828 2289 2974 3967 4158 33
Size: 252 543 1461 685 993 191 1121
Fragments arranged by size:
1461 1121 993 685 543 252 191
SfoI GGC'GCC
Cuts at: 2678 2926 2678
Size: 248 4998
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5246
SmaI CCC'GGG
Cuts at: 2268 2268
Size: 5246
SmlI C'TyrA_G
Cuts at: 907 2080 2401 3316 3808 4070 907
Size: 1173 321 915 492 262 2083
Fragments arranged by size:
2083 1173 915 492 321 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5246
SphI G_CATG'C
Cuts at: 1370 1752 2299 2799 1370
Size: 382 547 500 3817
Fragments arranged by size:
3817 547 500 382
SspI AAT'ATT
Cuts at: 840 840
Size: 5246
StuI AGG'CCT
Cuts at: 1471 1891 1947 2143 1471
Size: 420 56 196 4574
Fragments arranged by size:
4574 420 196 56
StyI C'CwwG_G
Cuts at: 2380 2734 2380
Size: 354 4892
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 988 1660 2829 2962 3421 3630 988
Size: 672 1169 133 459 209 2604
Fragments arranged by size:
2604 1169 672 459 209 133
TatI w'GTAC_w
Cuts at: 4761 4761
Size: 5246
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 5214
Tth111I GACn'n_nGTC
Cuts at: 3043 3043
Size: 5246
XbaI T'CTAG_A
Cuts at: 2277 2277
Size: 5246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2741 2741
Size: 5246
XmaI C'CCGG_G
Cuts at: 2266 2266
Size: 5246
XmnI GAAnn'nnTTC
Cuts at: 1555 1723 1807 1891 2059 1555
Size: 168 84 84 168 4742
Fragments arranged by size:
4742 168 168 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI
DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI PacI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI