Restriction map for
pF1KB6203
(Circular) (Six-base) MAPSORT of: pF1KB6203.seq Check: 4411 from: 1 to: 5102
pF1KB6203 5102 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1461 1461
Size: 5102
AccI GT'mk_AC
Cuts at: 2140 2140
Size: 5102
Acc65I G'GTAC_C
Cuts at: 2118 2118
Size: 5102
AclI AA'CG_TT
Cuts at: 4942 4942
Size: 5102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 1087 1255 1786 2927 3359 4106 4374 210
Size: 877 168 531 1141 432 747 268 938
Fragments arranged by size:
1141 938 877 747 531 432 268 168
AflIII A'CryG_T
Cuts at: 644 926 1034 1254 2454 3558 4535 644
Size: 282 108 220 1200 1104 977 1211
Fragments arranged by size:
1211 1200 1104 977 282 220 108
AgeI A'CCGG_T
Cuts at: 3490 3490
Size: 5102
AhdI GACnn_n'nnGTC
Cuts at: 219 1506 219
Size: 1287 3815
AleI CACnn'nnGTG
Cuts at: 442 2050 442
Size: 1608 3494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2068 2068
Size: 5102
AlwNI CAG_nnn'CTG
Cuts at: 1446 1595 2407 3974 1446
Size: 149 812 1567 2574
Fragments arranged by size:
2574 1567 812 149
ApaI G_GGCC'C
Cuts at: 535 1295 1919 535
Size: 760 624 3718
Fragments arranged by size:
3718 760 624
ApaLI G'TGCA_C
Cuts at: 1986 2035 3872 1986
Size: 49 1837 3216
Fragments arranged by size:
3216 1837 49
ApoI r'AATT_y
Cuts at: 2106 4455 4711 2106
Size: 2349 256 2497
Fragments arranged by size:
2497 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5102
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
AvaI C'yCGr_G
Cuts at: 891 946 1286 2122 891
Size: 55 340 836 3871
Fragments arranged by size:
3871 836 340 55
BamHI G'GATC_C
Cuts at: 2127 2127
Size: 5102
BanI G'GyrC_C
Cuts at: 172 547 987 2044 2091 2118 2532 2780 2815
Size: 375 440 1057 47 27 414 248 35
Cuts at: 2815 172
Size: 2459
Fragments arranged by size:
2459 1057 440 414 375 248 47 35
27
BanII G_rGCy'C
Cuts at: 535 899 944 1295 1348 1919 2116 3146 535
Size: 364 45 351 53 571 197 1030 2491
Fragments arranged by size:
2491 1030 571 364 351 197 53 45
BbeI G_GCGC'C
Cuts at: 991 2536 2784 991
Size: 1545 248 3309
Fragments arranged by size:
3309 1545 248
BbsI GAAGACnn'nnnn_
Cuts at: 638 676 969 1200 638
Size: 38 293 231 4540
Fragments arranged by size:
4540 293 231 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 574 608 574
Size: 34 5068
BciVI GTATCCnnnnn_n'
Cuts at: 3000 3761 5102 3000
Size: 761 1341 3000
Fragments arranged by size:
3000 1341 761
BclI T'GATC_A
Cuts at: 2622 2622
Size: 5102
BfrBI ATG'CAT
Cuts at: 1742 1742
Size: 5102
BglI GCCn_nnn'nGGC
Cuts at: 1914 1914
Size: 5102
BglII A'GATC_T
Cuts at: 2617 4654 2617
Size: 2037 3065
BlpI GC'TnA_GC
Cuts at: 2214 2214
Size: 5102
Bme1580I G_kGCm'C
Cuts at: 175 535 1295 1467 1919 1990 2039 2096 2727
Size: 360 760 172 452 71 49 57 631
Cuts at: 2727 2820 3876 175
Size: 93 1056 1401
Fragments arranged by size:
1401 1056 760 631 452 360 172 93
71 57 49
BmgBI CAC'GTC
Cuts at: 221 929 221
Size: 708 4394
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1984 2314 2395 2730 4874 1345
Size: 639 330 81 335 2144 1573
Fragments arranged by size:
2144 1573 639 335 330 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 598 3469 598
Size: 2871 2231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 958 1963 1966 1987 260
Size: 698 1005 3 21 3375
Fragments arranged by size:
3375 1005 698 21 3
Bpu10I CC'TnA_GC
Cuts at: 661 1534 661
Size: 873 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2278 3157 3649 3947 2278
Size: 879 492 298 3433
Fragments arranged by size:
3433 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4806 4806
Size: 5102
BsaAI yAC'GTr
Cuts at: 714 858 1521 2455 714
Size: 144 663 934 3361
Fragments arranged by size:
3361 934 663 144
BsaBI GATnn'nnATC
Cuts at: 6 2616 4653 6
Size: 2610 2037 455
Fragments arranged by size:
2610 2037 455
BsaHI Gr'CG_yC
Cuts at: 988 2533 2781 3483 4979 988
Size: 1545 248 702 1496 1111
Fragments arranged by size:
1545 1496 1111 702 248
BsaWI w'CCGG_w
Cuts at: 2291 2812 3490 3764 3911 4322 2291
Size: 521 678 274 147 411 3071
Fragments arranged by size:
3071 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 692 722 1939 1964 1969 1994 692
Size: 30 1217 25 5 25 3800
Fragments arranged by size:
3800 1217 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1138 1141 1960 1138
Size: 3 819 4280
Fragments arranged by size:
4280 819 3
BseYI C'CCAG_C
Cuts at: 168 1013 1380 1414 1466 1776 2527 3285 3862
Size: 845 367 34 52 310 751 758 577
Cuts at: 3862 4358 168
Size: 496 912
Fragments arranged by size:
912 845 758 751 577 496 367 310
52 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 801 1059 1495 201
Size: 600 258 436 3808
Fragments arranged by size:
3808 600 436 258
BsiEI CG_ry'CG
Cuts at: 104 1932 2644 2690 3898 104
Size: 1828 712 46 1208 1308
Fragments arranged by size:
1828 1308 1208 712 46
BsiHKAI G_wGCw'C
Cuts at: 667 710 944 1990 2039 2116 2894 3084 3876
Size: 43 234 1046 49 77 778 190 792
Cuts at: 3876 667
Size: 1893
Fragments arranged by size:
1893 1046 792 778 234 190 77 49
43
Bsp1286I G_dGCh'C
Cuts at: 175 535 667 710 899 944 1295 1348 1467
Size: 360 132 43 189 45 351 53 119
Cuts at: 1467 1919 1990 2039 2096 2116 2727 2820 2894
Size: 452 71 49 57 20 611 93 74
Cuts at: 2894 3084 3146 3876 175
Size: 190 62 730 1401
Fragments arranged by size:
1401 730 611 452 360 351 190 189
132 119 93 74 71 62 57 53
49 45 43 20
BspEI T'CCGG_A
Cuts at: 4322 4322
Size: 5102
BspHI T'CATG_A
Cuts at: 998 4278 998
Size: 3280 1822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 644 1461 2152 2366 2668 3049 644
Size: 817 691 214 302 381 2697
Fragments arranged by size:
2697 817 691 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3394 3448 4932 5097 50
Size: 3344 54 1484 165 55
Fragments arranged by size:
3344 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1425 3014 1425
Size: 1589 3513
BsrFI r'CCGG_y
Cuts at: 478 1427 3100 3281 3490 478
Size: 949 1673 181 209 2090
Fragments arranged by size:
2090 1673 949 209 181
BssSI C'ACGA_G
Cuts at: 3373 3731 3373
Size: 358 4744
BstAPI GCAn_nnn'nTGC
Cuts at: 2479 2479
Size: 5102
BstBI TT'CG_AA
Cuts at: 1652 3463 1652
Size: 1811 3291
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 5102
BstXI CCAn_nnnn'nTGG
Cuts at: 558 558
Size: 5102
BstYI r'GATC_y
Cuts at: 1 2127 2594 2617 2952 3198 4210 4296 4308
Size: 2126 467 23 335 246 1012 86 12
Cuts at: 4308 4654 1
Size: 346 449
Fragments arranged by size:
2126 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 209 1121 209
Size: 912 4190
BtgI C'CryG_G
Cuts at: 107 440 596 1202 1511 1632 107
Size: 333 156 606 309 121 3577
Fragments arranged by size:
3577 606 333 309 156 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3052 3233 3396 3052
Size: 181 163 4758
Fragments arranged by size:
4758 181 163
BtsI GCAGTG_nn'
Cuts at: 2037 2345 2037
Size: 308 4794
DraI TTT'AAA
Cuts at: 2101 2101
Size: 5102
DrdI GACnn_nn'nnGTC
Cuts at: 973 2808 3666 973
Size: 1835 858 2409
Fragments arranged by size:
2409 1835 858
EaeI y'GGCC_r
Cuts at: 353 716 1085 1580 1784 1929 2687 2861 3252
Size: 363 369 495 204 145 758 174 391
Cuts at: 3252 3279 4624 353
Size: 27 1345 831
Fragments arranged by size:
1345 831 758 495 391 369 363 204
174 145 27
EagI C'GGCC_G
Cuts at: 1929 2687 1929
Size: 758 4344
EarI CTCTTCn'nnn_
Cuts at: 1399 1822 3125 3335 1399
Size: 423 1303 210 3166
Fragments arranged by size:
3166 1303 423 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 3310 3620 3766 4912 1858
Size: 1452 310 146 1146 2048
Fragments arranged by size:
2048 1452 1146 310 146
EcoICRI GAG'CTC
Cuts at: 942 2114 942
Size: 1172 3930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 260 958 1087 1255 1786 1963 1966 1987
Size: 50 698 129 168 531 177 3 21
Cuts at: 1987 2927 3359 4106 4374 210
Size: 940 432 747 268 938
Fragments arranged by size:
940 938 747 698 531 432 268 177
168 129 50 21 3
EcoO109I rG'GnC_Cy
Cuts at: 518 531 532 796 864 1291 1292 1443 1479
Size: 13 1 264 68 427 1 151 36
Cuts at: 1479 1592 1641 2241 518
Size: 113 49 600 3379
Fragments arranged by size:
3379 600 427 264 151 113 68 49
36 13 1 1
EcoRI G'AATT_C
Cuts at: 2106 2106
Size: 5102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 702 3121 702
Size: 2419 2683
FspI TGC'GCA
Cuts at: 462 2056 2883 462
Size: 1594 827 2681
Fragments arranged by size:
2681 1594 827
HaeII r_GCGC'y
Cuts at: 991 2536 2784 3806 4785 991
Size: 1545 248 1022 979 1308
Fragments arranged by size:
1545 1308 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 1492 1524 2944 2974 2976 3006 205
Size: 32 1255 32 1420 30 2 30 2301
Fragments arranged by size:
2301 1420 1255 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2141 2141
Size: 5102
HindIII A'AGCT_T
Cuts at: 1847 1847
Size: 5102
KasI G'GCGC_C
Cuts at: 987 2532 2780 987
Size: 1545 248 3309
Fragments arranged by size:
3309 1545 248
KpnI G_GTAC'C
Cuts at: 2122 2122
Size: 5102
MluI A'CGCG_T
Cuts at: 1034 1254 4535 1034
Size: 220 3281 1601
Fragments arranged by size:
3281 1601 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 956 3773 3957 956
Size: 2817 184 2101
Fragments arranged by size:
2817 2101 184
MscI TGG'CCA
Cuts at: 718 1087 1582 2863 718
Size: 369 495 1281 2957
Fragments arranged by size:
2957 1281 495 369
MslI CAynn'nnrTG
Cuts at: 442 1876 2050 3091 3218 442
Size: 1434 174 1041 127 2326
Fragments arranged by size:
2326 1434 1041 174 127
MspA1I CmG'CkG
Cuts at: 442 1380 1406 1934 2213 2527 2887 3900 4145
Size: 938 26 528 279 314 360 1013 245
Cuts at: 4145 442
Size: 1399
Fragments arranged by size:
1399 1013 938 528 360 314 279 245
26
NaeI GCC'GGC
Cuts at: 1429 3283 1429
Size: 1854 3248
NarI GG'CG_CC
Cuts at: 988 2533 2781 988
Size: 1545 248 3309
Fragments arranged by size:
3309 1545 248
NcoI C'CATG_G
Cuts at: 107 596 107
Size: 489 4613
NgoMIV G'CCGG_C
Cuts at: 1427 3281 1427
Size: 1854 3248
NsiI A_TGCA'T
Cuts at: 1744 1744
Size: 5102
NspI r_CATG'y
Cuts at: 648 1875 2155 2655 3562 648
Size: 1227 280 500 907 2188
Fragments arranged by size:
2188 1227 907 500 280
PasI CC'CwG_GG
Cuts at: 114 244 322 114
Size: 130 78 4894
Fragments arranged by size:
4894 130 78
PciI A'CATG_T
Cuts at: 644 3558 644
Size: 2914 2188
PflMI CCAn_nnn'nTGG
Cuts at: 702 1273 702
Size: 571 4531
PfoI T'CCnGG_A
Cuts at: 875 1610 875
Size: 735 4367
PmeI GTTT'AAAC
Cuts at: 2101 2101
Size: 5102
PmlI CAC'GTG
Cuts at: 714 1521 714
Size: 807 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 4241
Size: 32 5070
PpuMI rG'GwC_Cy
Cuts at: 518 796 1592 518
Size: 278 796 4028
Fragments arranged by size:
4028 796 278
PspOMI G'GGCC_C
Cuts at: 531 1291 1915 531
Size: 760 624 3718
Fragments arranged by size:
3718 760 624
PspXI vC'TCGA_Gb
Cuts at: 1286 1286
Size: 5102
PstI C_TGCA'G
Cuts at: 199 641 1451 2149 2834 199
Size: 442 810 698 685 2467
Fragments arranged by size:
2467 810 698 685 442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5102
PvuII CAG'CTG
Cuts at: 1406 2527 2887 1406
Size: 1121 360 3621
Fragments arranged by size:
3621 1121 360
RsrII CG'GwC_CG
Cuts at: 3297 3297
Size: 5102
SacI G_AGCT'C
Cuts at: 944 2116 944
Size: 1172 3930
SacII CC_GC'GG
Cuts at: 443 443
Size: 5102
SalI G'TCGA_C
Cuts at: 2139 2139
Size: 5102
SanDI GG'GwC_CC
Cuts at: 796 796
Size: 5102
SapI GCTCTTCn'nnn_
Cuts at: 1822 3125 3335 1822
Size: 1303 210 3589
Fragments arranged by size:
3589 1303 210
SbfI CC_TGCA'GG
Cuts at: 641 1451 2149 641
Size: 810 698 3594
Fragments arranged by size:
3594 810 698
ScaI AGT'ACT
Cuts at: 4619 4619
Size: 5102
SfcI C'TryA_G
Cuts at: 33 195 637 1447 2145 2830 3823 4014 33
Size: 162 442 810 698 685 993 191 1121
Fragments arranged by size:
1121 993 810 698 685 442 191 162
SfoI GGC'GCC
Cuts at: 989 2534 2782 989
Size: 1545 248 3309
Fragments arranged by size:
3309 1545 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
SmaI CCC'GGG
Cuts at: 893 2124 893
Size: 1231 3871
SmlI C'TyrA_G
Cuts at: 1286 2257 3172 3664 3926 1286
Size: 971 915 492 262 2462
Fragments arranged by size:
2462 971 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5102
SphI G_CATG'C
Cuts at: 2155 2655 2155
Size: 500 4602
StuI AGG'CCT
Cuts at: 1232 1232
Size: 5102
StyI C'CwwG_G
Cuts at: 107 596 677 1219 2236 2590 107
Size: 489 81 542 1017 354 2619
Fragments arranged by size:
2619 1017 542 489 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 1027 2685 2818 3277 3486 166
Size: 861 1658 133 459 209 1782
Fragments arranged by size:
1782 1658 861 459 209 133
TatI w'GTAC_w
Cuts at: 4617 4617
Size: 5102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 692 724 1129 1161 1939 1964 1971 1996 4619
Size: 32 405 32 778 25 7 25 2623
Cuts at: 4619 4651 692
Size: 32 1143
Fragments arranged by size:
2623 1143 778 405 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 2899 2899
Size: 5102
XbaI T'CTAG_A
Cuts at: 2133 2133
Size: 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 312 726 918 2023 2597 312
Size: 414 192 1105 574 2817
Fragments arranged by size:
2817 1105 574 414 192
XhoI C'TCGA_G
Cuts at: 1286 1286
Size: 5102
XmaI C'CCGG_G
Cuts at: 891 2122 891
Size: 1231 3871
XmnI GAAnn'nnTTC
Cuts at: 1564 1564
Size: 5102
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbvCI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI