Restriction map for
pF1KB6205
(Circular) (Six-base) MAPSORT of: pF1KB6205.seq Check: 6532 from: 1 to: 5105
pF1KB6205 5105 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1416 2143 1416
Size: 727 4378
Acc65I G'GTAC_C
Cuts at: 2121 2121
Size: 5105
AclI AA'CG_TT
Cuts at: 4945 4945
Size: 5105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1442 1658 2930 3362 4109 4377 628
Size: 814 216 1272 432 747 268 1356
Fragments arranged by size:
1356 1272 814 747 432 268 216
AflIII A'CryG_T
Cuts at: 2457 3561 4538 2457
Size: 1104 977 3024
Fragments arranged by size:
3024 1104 977
AgeI A'CCGG_T
Cuts at: 3493 3493
Size: 5105
AlwNI CAG_nnn'CTG
Cuts at: 904 1080 1356 2410 3977 904
Size: 176 276 1054 1567 2032
Fragments arranged by size:
2032 1567 1054 276 176
ApaLI G'TGCA_C
Cuts at: 3875 3875
Size: 5105
ApoI r'AATT_y
Cuts at: 1362 1486 2109 4458 4714 1362
Size: 124 623 2349 256 1753
Fragments arranged by size:
2349 1753 623 256 124
AseI AT'TA_AT
Cuts at: 20 1934 1990 20
Size: 1914 56 3135
Fragments arranged by size:
3135 1914 56
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
AvaI C'yCGr_G
Cuts at: 1676 2125 1676
Size: 449 4656
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1748 1781 1748
Size: 33 5072
BamHI G'GATC_C
Cuts at: 1968 2130 1968
Size: 162 4943
BanI G'GyrC_C
Cuts at: 199 2121 2535 2783 2818 199
Size: 1922 414 248 35 2486
Fragments arranged by size:
2486 1922 414 248 35
BanII G_rGCy'C
Cuts at: 258 704 2119 3149 258
Size: 446 1415 1030 2214
Fragments arranged by size:
2214 1415 1030 446
BbeI G_GCGC'C
Cuts at: 2539 2787 2539
Size: 248 4857
BbsI GAAGACnn'nnnn_
Cuts at: 592 1121 592
Size: 529 4576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5071
BciVI GTATCCnnnnn_n'
Cuts at: 3003 3764 5105 3003
Size: 761 1341 3003
Fragments arranged by size:
3003 1341 761
BclI T'GATC_A
Cuts at: 1494 2625 1494
Size: 1131 3974
BglII A'GATC_T
Cuts at: 1235 2620 4657 1235
Size: 1385 2037 1683
Fragments arranged by size:
2037 1683 1385
BlpI GC'TnA_GC
Cuts at: 2217 2217
Size: 5105
Bme1580I G_kGCm'C
Cuts at: 660 2730 2823 3879 660
Size: 2070 93 1056 1886
Fragments arranged by size:
2070 1886 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2317 2398 2733 4877 2317
Size: 81 335 2144 2545
Fragments arranged by size:
2545 2144 335 81
BmtI G_CTAG'C
Cuts at: 518 518
Size: 5105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3472 3472
Size: 5105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 881 1082 1156 1212 1886 214
Size: 667 201 74 56 674 3433
Fragments arranged by size:
3433 674 667 201 74 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2281 3160 3652 3950 1745
Size: 536 879 492 298 2900
Fragments arranged by size:
2900 879 536 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4809 4809
Size: 5105
BsaAI yAC'GTr
Cuts at: 2458 2458
Size: 5105
BsaBI GATnn'nnATC
Cuts at: 6 2063 2619 4656 6
Size: 2057 556 2037 455
Fragments arranged by size:
2057 2037 556 455
BsaHI Gr'CG_yC
Cuts at: 2536 2784 3486 4982 2536
Size: 248 702 1496 2659
Fragments arranged by size:
2659 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2294 2815 3493 3767 3914 4325 2294
Size: 521 678 274 147 411 3074
Fragments arranged by size:
3074 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 622 652 1210 1240 1500 1530 622
Size: 30 558 30 260 30 4197
Fragments arranged by size:
4197 558 260 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 627 1444 151
Size: 476 817 3812
Fragments arranged by size:
3812 817 476
BseYI C'CCAG_C
Cuts at: 857 1345 2530 3288 3865 4361 857
Size: 488 1185 758 577 496 1601
Fragments arranged by size:
1601 1185 758 577 496 488
BsiEI CG_ry'CG
Cuts at: 104 2647 2693 3901 104
Size: 2543 46 1208 1308
Fragments arranged by size:
2543 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2119 2897 3087 3879 2119
Size: 778 190 792 3345
Fragments arranged by size:
3345 792 778 190
BsmI GAATG_Cn'
Cuts at: 812 812
Size: 5105
Bsp1286I G_dGCh'C
Cuts at: 258 660 704 2119 2730 2823 2897 3087 3149
Size: 402 44 1415 611 93 74 190 62
Cuts at: 3149 3879 258
Size: 730 1484
Fragments arranged by size:
1484 1415 730 611 402 190 93 74
62 44
BspEI T'CCGG_A
Cuts at: 4325 4325
Size: 5105
BspHI T'CATG_A
Cuts at: 4281 4281
Size: 5105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2155 2369 2671 3052 2155
Size: 214 302 381 4208
Fragments arranged by size:
4208 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1288 3397 3451 4935 5100 50
Size: 1238 2109 54 1484 165 55
Fragments arranged by size:
2109 1484 1238 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 525 3017 242
Size: 283 2492 2330
Fragments arranged by size:
2492 2330 283
BsrFI r'CCGG_y
Cuts at: 1118 3103 3284 3493 1118
Size: 1985 181 209 2730
Fragments arranged by size:
2730 1985 209 181
BssSI C'ACGA_G
Cuts at: 3376 3734 3376
Size: 358 4747
BstAPI GCAn_nnn'nTGC
Cuts at: 2482 2482
Size: 5105
BstBI TT'CG_AA
Cuts at: 3466 3466
Size: 5105
BstEII G'GTnAC_C
Cuts at: 952 984 1722 952
Size: 32 738 4335
Fragments arranged by size:
4335 738 32
BstXI CCAn_nnnn'nTGG
Cuts at: 384 771 949 384
Size: 387 178 4540
Fragments arranged by size:
4540 387 178
BstYI r'GATC_y
Cuts at: 1 471 498 1235 1749 1968 2130 2597 2620
Size: 470 27 737 514 219 162 467 23
Cuts at: 2620 2955 3201 4213 4299 4311 4657 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 737 514 470 467 449 346 335
246 219 162 86 27 23 12
Bsu36I CC'TnA_GG
Cuts at: 1256 1256
Size: 5105
BtgI C'CryG_G
Cuts at: 116 611 720 1055 1103 116
Size: 495 109 335 48 4118
Fragments arranged by size:
4118 495 335 109 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 3055 3236 3399 1669
Size: 1386 181 163 3375
Fragments arranged by size:
3375 1386 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 2348 1304
Size: 1044 4061
DraI TTT'AAA
Cuts at: 494 2104 494
Size: 1610 3495
DrdI GACnn_nn'nnGTC
Cuts at: 2811 3669 2811
Size: 858 4247
EaeI y'GGCC_r
Cuts at: 275 608 689 911 1100 2690 2864 3255 3282
Size: 333 81 222 189 1590 174 391 27
Cuts at: 3282 4627 275
Size: 1345 753
Fragments arranged by size:
1590 1345 753 391 333 222 189 174
81 27
EagI C'GGCC_G
Cuts at: 2690 2690
Size: 5105
EarI CTCTTCn'nnn_
Cuts at: 1193 3128 3338 1193
Size: 1935 210 2960
Fragments arranged by size:
2960 1935 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 636 853 3313 3623 3769 4915 636
Size: 217 2460 310 146 1146 826
Fragments arranged by size:
2460 1146 826 310 217 146
EcoICRI GAG'CTC
Cuts at: 2117 2117
Size: 5105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 628 881 1082 1156 1212 1442 1658 1886
Size: 414 253 201 74 56 230 216 228
Cuts at: 1886 2930 3362 4109 4377 214
Size: 1044 432 747 268 942
Fragments arranged by size:
1044 942 747 432 414 268 253 230
228 216 201 74 56
EcoNI CCTnn'n_nnAGG
Cuts at: 133 999 133
Size: 866 4239
EcoO109I rG'GnC_Cy
Cuts at: 1077 2244 1077
Size: 1167 3938
EcoRI G'AATT_C
Cuts at: 1486 2109 1486
Size: 623 4482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 886 1588 3124 886
Size: 702 1536 2867
Fragments arranged by size:
2867 1536 702
FspI TGC'GCA
Cuts at: 2886 2886
Size: 5105
HaeII r_GCGC'y
Cuts at: 1125 2539 2787 3809 4788 1125
Size: 1414 248 1022 979 1442
Fragments arranged by size:
1442 1414 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1055 1075 1087 1107 1531 1563 2947 2977 2979
Size: 20 12 20 424 32 1384 30 2
Cuts at: 2979 3009 1055
Size: 30 3151
Fragments arranged by size:
3151 1384 424 32 30 30 20 20
12 2
HincII GTy'rAC
Cuts at: 2144 2144
Size: 5105
KasI G'GCGC_C
Cuts at: 2535 2783 2535
Size: 248 4857
KpnI G_GTAC'C
Cuts at: 2125 2125
Size: 5105
MluI A'CGCG_T
Cuts at: 4538 4538
Size: 5105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 576 3776 3960 576
Size: 3200 184 1721
Fragments arranged by size:
3200 1721 184
MscI TGG'CCA
Cuts at: 277 691 2866 277
Size: 414 2175 2516
Fragments arranged by size:
2516 2175 414
MslI CAynn'nnrTG
Cuts at: 3094 3221 3094
Size: 127 4978
MspA1I CmG'CkG
Cuts at: 225 613 2216 2530 2890 3903 4148 225
Size: 388 1603 314 360 1013 245 1182
Fragments arranged by size:
1603 1182 1013 388 360 314 245
NaeI GCC'GGC
Cuts at: 3286 3286
Size: 5105
NarI GG'CG_CC
Cuts at: 2536 2784 2536
Size: 248 4857
NcoI C'CATG_G
Cuts at: 720 720
Size: 5105
NdeI CA'TA_TG
Cuts at: 309 309
Size: 5105
NgoMIV G'CCGG_C
Cuts at: 3284 3284
Size: 5105
NheI G'CTAG_C
Cuts at: 514 514
Size: 5105
NruI TCG'CGA
Cuts at: 1267 1267
Size: 5105
NspI r_CATG'y
Cuts at: 2158 2658 3565 2158
Size: 500 907 3698
Fragments arranged by size:
3698 907 500
PasI CC'CwG_GG
Cuts at: 1046 1046
Size: 5105
PciI A'CATG_T
Cuts at: 3561 3561
Size: 5105
PflMI CCAn_nnn'nTGG
Cuts at: 995 1080 1719 995
Size: 85 639 4381
Fragments arranged by size:
4381 639 85
PfoI T'CCnGG_A
Cuts at: 1073 1073
Size: 5105
PmeI GTTT'AAAC
Cuts at: 2104 2104
Size: 5105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 5073
PpuMI rG'GwC_Cy
Cuts at: 1077 1077
Size: 5105
PshAI GACnn'nnGTC
Cuts at: 592 592
Size: 5105
PstI C_TGCA'G
Cuts at: 674 2152 2837 674
Size: 1478 685 2942
Fragments arranged by size:
2942 1478 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5105
PvuII CAG'CTG
Cuts at: 225 2530 2890 225
Size: 2305 360 2440
Fragments arranged by size:
2440 2305 360
RsrII CG'GwC_CG
Cuts at: 3300 3300
Size: 5105
SacI G_AGCT'C
Cuts at: 2119 2119
Size: 5105
SacII CC_GC'GG
Cuts at: 614 614
Size: 5105
SalI G'TCGA_C
Cuts at: 2142 2142
Size: 5105
SapI GCTCTTCn'nnn_
Cuts at: 3128 3338 3128
Size: 210 4895
SbfI CC_TGCA'GG
Cuts at: 2152 2152
Size: 5105
ScaI AGT'ACT
Cuts at: 649 4622 649
Size: 3973 1132
SfcI C'TryA_G
Cuts at: 33 670 1481 2148 2833 3826 4017 33
Size: 637 811 667 685 993 191 1121
Fragments arranged by size:
1121 993 811 685 667 637 191
SfoI GGC'GCC
Cuts at: 2537 2785 2537
Size: 248 4857
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
SmaI CCC'GGG
Cuts at: 2127 2127
Size: 5105
SmlI C'TyrA_G
Cuts at: 1760 2260 3175 3667 3929 1760
Size: 500 915 492 262 2936
Fragments arranged by size:
2936 915 500 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5105
SphI G_CATG'C
Cuts at: 2158 2658 2158
Size: 500 4605
StyI C'CwwG_G
Cuts at: 720 1020 2239 2593 720
Size: 300 1219 354 3232
Fragments arranged by size:
3232 1219 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 1033 1261 2688 2821 3280 3489 604
Size: 429 228 1427 133 459 209 2220
Fragments arranged by size:
2220 1427 459 429 228 209 133
TatI w'GTAC_w
Cuts at: 647 674 1882 2028 4620 647
Size: 27 1208 146 2592 1132
Fragments arranged by size:
2592 1208 1132 146 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 590 622 654 4622 4654 358
Size: 32 200 32 32 3968 32 809
Fragments arranged by size:
3968 809 200 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2902 2902
Size: 5105
XbaI T'CTAG_A
Cuts at: 2136 2136
Size: 5105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1053 2600 114
Size: 939 1547 2619
Fragments arranged by size:
2619 1547 939
XmaI C'CCGG_G
Cuts at: 2125 2125
Size: 5105
XmnI GAAnn'nnTTC
Cuts at: 1550 1550
Size: 5105
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BglI BmgBI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NotI
NsiI PacI PmlI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI