Restriction map for
pF1KB6206
(Circular) (Six-base) MAPSORT of: pF1KB6206.seq Check: 3644 from: 1 to: 5258
pF1KB6206 5258 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 2296 2296
Size: 5258
Acc65I G'GTAC_C
Cuts at: 2274 2274
Size: 5258
AclI AA'CG_TT
Cuts at: 806 5098 806
Size: 4292 966
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 927 1068 1425 1509 1761 1845 3083 3515 4262
Size: 141 357 84 252 84 1238 432 747
Cuts at: 4262 4530 927
Size: 268 1655
Fragments arranged by size:
1655 1238 747 432 357 268 252 141
84 84
AflIII A'CryG_T
Cuts at: 2610 3714 4691 2610
Size: 1104 977 3177
Fragments arranged by size:
3177 1104 977
AgeI A'CCGG_T
Cuts at: 3646 3646
Size: 5258
AleI CACnn'nnGTG
Cuts at: 2072 2072
Size: 5258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 165 165
Size: 5258
AlwNI CAG_nnn'CTG
Cuts at: 2563 4130 2563
Size: 1567 3691
ApaLI G'TGCA_C
Cuts at: 1503 4028 1503
Size: 2525 2733
ApoI r'AATT_y
Cuts at: 384 696 1391 1475 1727 1811 1979 2147 2262
Size: 312 695 84 252 84 168 168 115
Cuts at: 2262 4611 4867 384
Size: 2349 256 775
Fragments arranged by size:
2349 775 695 312 256 252 168 168
115 84 84
AseI AT'TA_AT
Cuts at: 20 710 20
Size: 690 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
AvaI C'yCGr_G
Cuts at: 347 2278 347
Size: 1931 3327
BamHI G'GATC_C
Cuts at: 2283 2283
Size: 5258
BanI G'GyrC_C
Cuts at: 2274 2688 2936 2971 2274
Size: 414 248 35 4561
Fragments arranged by size:
4561 414 248 35
BanII G_rGCy'C
Cuts at: 2041 2125 2272 3302 2041
Size: 84 147 1030 3997
Fragments arranged by size:
3997 1030 147 84
BbeI G_GCGC'C
Cuts at: 2692 2940 2692
Size: 248 5010
BciVI GTATCCnnnnn_n'
Cuts at: 3156 3917 5258 3156
Size: 761 1341 3156
Fragments arranged by size:
3156 1341 761
BclI T'GATC_A
Cuts at: 2778 2778
Size: 5258
BfrBI ATG'CAT
Cuts at: 2055 2055
Size: 5258
BglII A'GATC_T
Cuts at: 2773 4810 2773
Size: 2037 3221
BlpI GC'TnA_GC
Cuts at: 2370 2370
Size: 5258
Bme1580I G_kGCm'C
Cuts at: 1507 2883 2976 4032 1507
Size: 1376 93 1056 2733
Fragments arranged by size:
2733 1376 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1997 2470 2551 2886 5030 1997
Size: 473 81 335 2144 2225
Fragments arranged by size:
2225 2144 473 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 477 3625 477
Size: 3148 2110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 325 1091 1253 1337 1421 1505 1589 1673
Size: 66 766 162 84 84 84 84 84
Cuts at: 1673 1757 1841 1925 2177 2261 259
Size: 84 84 84 252 84 3256
Fragments arranged by size:
3256 766 252 162 84 84 84 84
84 84 84 84 84 66
Bpu10I CC'TnA_GC
Cuts at: 662 662
Size: 5258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 546 810 2434 3313 3805 4103 388
Size: 158 264 1624 879 492 298 1543
Fragments arranged by size:
1624 1543 879 492 298 264 158
BsaI GGTCTCn'nnnn_
Cuts at: 235 1069 4962 235
Size: 834 3893 531
Fragments arranged by size:
3893 834 531
BsaAI yAC'GTr
Cuts at: 426 2611 426
Size: 2185 3073
BsaBI GATnn'nnATC
Cuts at: 6 2772 4809 6
Size: 2766 2037 455
Fragments arranged by size:
2766 2037 455
BsaHI Gr'CG_yC
Cuts at: 2689 2937 3639 5135 2689
Size: 248 702 1496 2812
Fragments arranged by size:
2812 1496 702 248
BsaWI w'CCGG_w
Cuts at: 447 2447 2968 3646 3920 4067 4478 447
Size: 2000 521 678 274 147 411 1227
Fragments arranged by size:
2000 1227 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 1984 2014 364
Size: 30 1590 30 3608
Fragments arranged by size:
3608 1590 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 385 466 199
Size: 186 81 4991
Fragments arranged by size:
4991 186 81
BseYI C'CCAG_C
Cuts at: 2683 3441 4018 4514 2683
Size: 758 577 496 3427
Fragments arranged by size:
3427 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1035 345
Size: 690 4568
BsiEI CG_ry'CG
Cuts at: 104 2800 2846 4054 104
Size: 2696 46 1208 1308
Fragments arranged by size:
2696 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1507 2041 2125 2272 3050 3240 4032 1507
Size: 534 84 147 778 190 792 2733
Fragments arranged by size:
2733 792 778 534 190 147 84
BsiWI C'GTAC_G
Cuts at: 1832 1832
Size: 5258
BsmI GAATG_Cn'
Cuts at: 172 1432 172
Size: 1260 3998
Bsp1286I G_dGCh'C
Cuts at: 1507 2041 2125 2272 2883 2976 3050 3240 3302
Size: 534 84 147 611 93 74 190 62
Cuts at: 3302 4032 1507
Size: 730 2733
Fragments arranged by size:
2733 730 611 534 190 147 93 84
74 62
BspEI T'CCGG_A
Cuts at: 4478 4478
Size: 5258
BspHI T'CATG_A
Cuts at: 714 4434 714
Size: 3720 1538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2308 2522 2824 3205 2308
Size: 214 302 381 4361
Fragments arranged by size:
4361 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3550 3604 5088 5253 50
Size: 3500 54 1484 165 55
Fragments arranged by size:
3500 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3170 3170
Size: 5258
BsrFI r'CCGG_y
Cuts at: 3256 3437 3646 3256
Size: 181 209 4868
Fragments arranged by size:
4868 209 181
BsrGI T'GTAC_A
Cuts at: 434 1047 1464 1632 434
Size: 613 417 168 4060
Fragments arranged by size:
4060 613 417 168
BssSI C'ACGA_G
Cuts at: 3529 3887 3529
Size: 358 4900
BstAPI GCAn_nnn'nTGC
Cuts at: 2015 2635 2015
Size: 620 4638
BstBI TT'CG_AA
Cuts at: 1247 3619 1247
Size: 2372 2886
BstYI r'GATC_y
Cuts at: 1 1366 2283 2750 2773 3108 3354 4366 4452
Size: 1365 917 467 23 335 246 1012 86
Cuts at: 4452 4464 4810 1
Size: 12 346 449
Fragments arranged by size:
1365 1012 917 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1115 107
Size: 1008 4250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3208 3389 3552 3208
Size: 181 163 4914
Fragments arranged by size:
4914 181 163
BtsI GCAGTG_nn'
Cuts at: 2501 2501
Size: 5258
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 446 481 1662 1697 446
Size: 35 1181 35 4007
Fragments arranged by size:
4007 1181 35 35
DraI TTT'AAA
Cuts at: 2257 2257
Size: 5258
DraIII CAC_nnn'GTG
Cuts at: 426 954 426
Size: 528 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2964 3822 2964
Size: 858 4400
EaeI y'GGCC_r
Cuts at: 2843 3017 3408 3435 4780 2843
Size: 174 391 27 1345 3321
Fragments arranged by size:
3321 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2843 2843
Size: 5258
EarI CTCTTCn'nnn_
Cuts at: 308 946 3281 3491 308
Size: 638 2335 210 2075
Fragments arranged by size:
2335 2075 638 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2244 3466 3776 3922 5068 2244
Size: 1222 310 146 1146 2434
Fragments arranged by size:
2434 1222 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2039 2123 2270 2039
Size: 84 147 5027
Fragments arranged by size:
5027 147 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 325 927 1068 1091 1253 1337 1421 1425
Size: 66 602 141 23 162 84 84 4
Cuts at: 1425 1505 1509 1589 1673 1757 1761 1841 1845
Size: 80 4 80 84 84 4 80 4
Cuts at: 1845 1925 2177 2261 3083 3515 4262 4530 259
Size: 80 252 84 822 432 747 268 987
Fragments arranged by size:
987 822 747 602 432 268 252 162
141 84 84 84 84 84 80 80
80 80 66 23 4 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 2031
Size: 5258
EcoO109I rG'GnC_Cy
Cuts at: 318 342 2397 318
Size: 24 2055 3179
Fragments arranged by size:
3179 2055 24
EcoRI G'AATT_C
Cuts at: 384 1391 1475 1727 1811 2147 2262 384
Size: 1007 84 252 84 336 115 3380
Fragments arranged by size:
3380 1007 336 252 115 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 382 3277 382
Size: 2895 2363
FspI TGC'GCA
Cuts at: 3039 3039
Size: 5258
HaeII r_GCGC'y
Cuts at: 2692 2940 3962 4941 2692
Size: 248 1022 979 3009
Fragments arranged by size:
3009 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 1150 1182 3100 3130 3132 3162 280
Size: 32 838 32 1918 30 2 30 2376
Fragments arranged by size:
2376 1918 838 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2297 2297
Size: 5258
KasI G'GCGC_C
Cuts at: 2688 2936 2688
Size: 248 5010
KpnI G_GTAC'C
Cuts at: 2278 2278
Size: 5258
MluI A'CGCG_T
Cuts at: 4691 4691
Size: 5258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3929 4113 3929
Size: 184 5074
MscI TGG'CCA
Cuts at: 3019 3019
Size: 5258
MslI CAynn'nnrTG
Cuts at: 147 758 1170 2072 3247 3374 147
Size: 611 412 902 1175 127 2031
Fragments arranged by size:
2031 1175 902 611 412 127
MspA1I CmG'CkG
Cuts at: 198 303 1199 2369 2683 3043 4056 4301 198
Size: 105 896 1170 314 360 1013 245 1155
Fragments arranged by size:
1170 1155 1013 896 360 314 245 105
NaeI GCC'GGC
Cuts at: 3439 3439
Size: 5258
NarI GG'CG_CC
Cuts at: 2689 2937 2689
Size: 248 5010
NcoI C'CATG_G
Cuts at: 107 1115 107
Size: 1008 4250
NgoMIV G'CCGG_C
Cuts at: 3437 3437
Size: 5258
NsiI A_TGCA'T
Cuts at: 2057 2057
Size: 5258
NspI r_CATG'y
Cuts at: 2311 2811 3718 2311
Size: 500 907 3851
Fragments arranged by size:
3851 907 500
PasI CC'CwG_GG
Cuts at: 264 264
Size: 5258
PciI A'CATG_T
Cuts at: 3714 3714
Size: 5258
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 5258
PmeI GTTT'AAAC
Cuts at: 2257 2257
Size: 5258
PmlI CAC'GTG
Cuts at: 426 426
Size: 5258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 4397
Size: 32 5226
PpuMI rG'GwC_Cy
Cuts at: 318 318
Size: 5258
PstI C_TGCA'G
Cuts at: 2305 2990 2305
Size: 685 4573
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5258
PvuII CAG'CTG
Cuts at: 198 303 1199 2683 3043 198
Size: 105 896 1484 360 2413
Fragments arranged by size:
2413 1484 896 360 105
RsrII CG'GwC_CG
Cuts at: 3453 3453
Size: 5258
SacI G_AGCT'C
Cuts at: 2041 2125 2272 2041
Size: 84 147 5027
Fragments arranged by size:
5027 147 84
SalI G'TCGA_C
Cuts at: 2295 2295
Size: 5258
SapI GCTCTTCn'nnn_
Cuts at: 3281 3491 3281
Size: 210 5048
SbfI CC_TGCA'GG
Cuts at: 2305 2305
Size: 5258
ScaI AGT'ACT
Cuts at: 4775 4775
Size: 5258
SfcI C'TryA_G
Cuts at: 33 1154 2301 2986 3979 4170 33
Size: 1121 1147 685 993 191 1121
Fragments arranged by size:
1147 1121 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2690 2938 2690
Size: 248 5010
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
SmaI CCC'GGG
Cuts at: 2280 2280
Size: 5258
SmlI C'TyrA_G
Cuts at: 367 525 789 2413 3328 3820 4082 367
Size: 158 264 1624 915 492 262 1543
Fragments arranged by size:
1624 1543 915 492 264 262 158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5258
SphI G_CATG'C
Cuts at: 2311 2811 2311
Size: 500 4758
StyI C'CwwG_G
Cuts at: 107 1115 2392 2746 107
Size: 1008 1277 354 2619
Fragments arranged by size:
2619 1277 1008 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1375 2841 2974 3433 3642 1375
Size: 1466 133 459 209 2991
Fragments arranged by size:
2991 1466 459 209 133
TatI w'GTAC_w
Cuts at: 434 649 1047 1252 1464 1588 1632 4773 434
Size: 215 398 205 212 124 44 3141 919
Fragments arranged by size:
3141 919 398 215 212 205 124 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 4775 4807 729
Size: 32 4014 32 1180
Fragments arranged by size:
4014 1180 32 32
Tth111I GACn'n_nGTC
Cuts at: 1291 3055 1291
Size: 1764 3494
XbaI T'CTAG_A
Cuts at: 2289 2289
Size: 5258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2753 2753
Size: 5258
XmaI C'CCGG_G
Cuts at: 2278 2278
Size: 5258
XmnI GAAnn'nnTTC
Cuts at: 897 1354 1606 1690 1774 2151 2194 897
Size: 457 252 84 84 377 43 3961
Fragments arranged by size:
3961 457 377 252 84 84 43
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PfoI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI