Restriction map for
pF1KB6207
(Circular) (Six-base) MAPSORT of: pF1KB6207.seq Check: 474 from: 1 to: 5108
pF1KB6207 5108 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2146 2146
Size: 5108
Acc65I G'GTAC_C
Cuts at: 2124 2124
Size: 5108
AclI AA'CG_TT
Cuts at: 4948 4948
Size: 5108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 631 1215 1551 1606 1774 1803 1971 2933
Size: 75 584 336 55 168 29 168 962
Cuts at: 2933 3365 4112 4380 556
Size: 432 747 268 1284
Fragments arranged by size:
1284 962 747 584 432 336 268 168
168 75 55 29
AflIII A'CryG_T
Cuts at: 1929 2460 3564 4541 1929
Size: 531 1104 977 2496
Fragments arranged by size:
2496 1104 977 531
AgeI A'CCGG_T
Cuts at: 3496 3496
Size: 5108
AhdI GACnn_n'nnGTC
Cuts at: 1724 1724
Size: 5108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1299 1299
Size: 5108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1067 1099 1067
Size: 32 5076
AlwNI CAG_nnn'CTG
Cuts at: 2413 3980 2413
Size: 1567 3541
ApaI G_GGCC'C
Cuts at: 2093 2093
Size: 5108
ApaLI G'TGCA_C
Cuts at: 1675 3878 1675
Size: 2203 2905
ApoI r'AATT_y
Cuts at: 1097 1140 2112 4461 4717 1097
Size: 43 972 2349 256 1488
Fragments arranged by size:
2349 1488 972 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 5108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
AvaI C'yCGr_G
Cuts at: 479 1174 2128 479
Size: 695 954 3459
Fragments arranged by size:
3459 954 695
BamHI G'GATC_C
Cuts at: 2133 2133
Size: 5108
BanI G'GyrC_C
Cuts at: 444 2124 2538 2786 2821 444
Size: 1680 414 248 35 2731
Fragments arranged by size:
2731 1680 414 248 35
BanII G_rGCy'C
Cuts at: 1829 2093 2122 3152 1829
Size: 264 29 1030 3785
Fragments arranged by size:
3785 1030 264 29
BbeI G_GCGC'C
Cuts at: 2542 2790 2542
Size: 248 4860
BbsI GAAGACnn'nnnn_
Cuts at: 1270 1594 1904 1270
Size: 324 310 4474
Fragments arranged by size:
4474 324 310
BbvCI CC'TCA_GC
Cuts at: 1016 1016
Size: 5108
BciVI GTATCCnnnnn_n'
Cuts at: 1679 3006 3767 5108 1679
Size: 1327 761 1341 1679
Fragments arranged by size:
1679 1341 1327 761
BclI T'GATC_A
Cuts at: 837 2628 837
Size: 1791 3317
BfrBI ATG'CAT
Cuts at: 1207 1879 1207
Size: 672 4436
BglI GCCn_nnn'nGGC
Cuts at: 832 832
Size: 5108
BglII A'GATC_T
Cuts at: 2623 4660 2623
Size: 2037 3071
BlpI GC'TnA_GC
Cuts at: 2220 2220
Size: 5108
Bme1580I G_kGCm'C
Cuts at: 449 1161 1175 1679 2093 2733 2826 3882 449
Size: 712 14 504 414 640 93 1056 1675
Fragments arranged by size:
1675 1056 712 640 504 414 93 14
BmrI ACTGGGnnnn_n'
Cuts at: 248 592 2320 2401 2736 4880 248
Size: 344 1728 81 335 2144 476
Fragments arranged by size:
2144 1728 476 344 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 328 1048 3475 328
Size: 720 2427 1961
Fragments arranged by size:
2427 1961 720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 381 484 826 1043 1127 1547 1799 213
Size: 168 103 342 217 84 420 252 3522
Fragments arranged by size:
3522 420 342 252 217 168 103 84
Bpu10I CC'TnA_GC
Cuts at: 1016 1016
Size: 5108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1755 2284 3163 3655 3953 1072
Size: 683 529 879 492 298 2227
Fragments arranged by size:
2227 879 683 529 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 521 1947 2032 4812 521
Size: 1426 85 2780 817
Fragments arranged by size:
2780 1426 817 85
BsaAI yAC'GTr
Cuts at: 631 1512 2461 631
Size: 881 949 3278
Fragments arranged by size:
3278 949 881
BsaBI GATnn'nnATC
Cuts at: 6 2622 4659 6
Size: 2616 2037 455
Fragments arranged by size:
2616 2037 455
BsaHI Gr'CG_yC
Cuts at: 908 2539 2787 3489 4985 908
Size: 1631 248 702 1496 1031
Fragments arranged by size:
1631 1496 1031 702 248
BsaWI w'CCGG_w
Cuts at: 1357 1652 2297 2818 3496 3770 3917 4328 1357
Size: 295 645 521 678 274 147 411 2137
Fragments arranged by size:
2137 678 645 521 411 295 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1690 1720 1690
Size: 30 5078
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 195 795 896 1989 195
Size: 600 101 1093 3314
Fragments arranged by size:
3314 1093 600 101
BseYI C'CCAG_C
Cuts at: 266 270 2533 3291 3868 4364 266
Size: 4 2263 758 577 496 1010
Fragments arranged by size:
2263 1010 758 577 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 611 947 234
Size: 377 336 4395
Fragments arranged by size:
4395 377 336
BsiEI CG_ry'CG
Cuts at: 104 2650 2696 3904 104
Size: 2546 46 1208 1308
Fragments arranged by size:
2546 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1663 1679 2122 2900 3090 3882 1663
Size: 16 443 778 190 792 2889
Fragments arranged by size:
2889 792 778 443 190 16
BsmI GAATG_Cn'
Cuts at: 1549 1633 1894 1549
Size: 84 261 4763
Fragments arranged by size:
4763 261 84
Bsp1286I G_dGCh'C
Cuts at: 449 1161 1175 1663 1679 1829 2093 2122 2733
Size: 712 14 488 16 150 264 29 611
Cuts at: 2733 2826 2900 3090 3152 3882 449
Size: 93 74 190 62 730 1675
Fragments arranged by size:
1675 730 712 611 488 264 190 150
93 74 62 29 16 14
BspEI T'CCGG_A
Cuts at: 1652 4328 1652
Size: 2676 2432
BspHI T'CATG_A
Cuts at: 4284 4284
Size: 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2158 2372 2674 3055 2158
Size: 214 302 381 4211
Fragments arranged by size:
4211 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3400 3454 4938 5103 50
Size: 3350 54 1484 165 55
Fragments arranged by size:
3350 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 160 3020 160
Size: 2860 2248
BsrFI r'CCGG_y
Cuts at: 488 3106 3287 3496 488
Size: 2618 181 209 2100
Fragments arranged by size:
2618 2100 209 181
BsrGI T'GTAC_A
Cuts at: 1926 2010 1926
Size: 84 5024
BssSI C'ACGA_G
Cuts at: 1401 3379 3737 1401
Size: 1978 358 2772
Fragments arranged by size:
2772 1978 358
BstAPI GCAn_nnn'nTGC
Cuts at: 444 2485 444
Size: 2041 3067
BstBI TT'CG_AA
Cuts at: 3469 3469
Size: 5108
BstXI CCAn_nnnn'nTGG
Cuts at: 755 1807 755
Size: 1052 4056
BstYI r'GATC_y
Cuts at: 1 375 983 2133 2600 2623 2958 3204 4216
Size: 374 608 1150 467 23 335 246 1012
Cuts at: 4216 4302 4314 4660 1
Size: 86 12 346 449
Fragments arranged by size:
1150 1012 608 467 449 374 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 430 1501 1837 430
Size: 1071 336 3701
Fragments arranged by size:
3701 1071 336
BtgI C'CryG_G
Cuts at: 107 137 598 107
Size: 30 461 4617
Fragments arranged by size:
4617 461 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3058 3239 3402 3058
Size: 181 163 4764
Fragments arranged by size:
4764 181 163
BtsI GCAGTG_nn'
Cuts at: 895 2351 895
Size: 1456 3652
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 309 344 1620 1655 309
Size: 35 1276 35 3762
Fragments arranged by size:
3762 1276 35 35
DraI TTT'AAA
Cuts at: 2107 2107
Size: 5108
DraIII CAC_nnn'GTG
Cuts at: 1945 1945
Size: 5108
DrdI GACnn_nn'nnGTC
Cuts at: 907 2814 3672 907
Size: 1907 858 2343
Fragments arranged by size:
2343 1907 858
EaeI y'GGCC_r
Cuts at: 134 490 605 2693 2867 3258 3285 4630 134
Size: 356 115 2088 174 391 27 1345 612
Fragments arranged by size:
2088 1345 612 391 356 174 115 27
EagI C'GGCC_G
Cuts at: 2693 2693
Size: 5108
EarI CTCTTCn'nnn_
Cuts at: 350 452 1584 1750 2028 3131 3341 350
Size: 102 1132 166 278 1103 210 2117
Fragments arranged by size:
2117 1132 1103 278 210 166 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3316 3626 3772 4918 3316
Size: 310 146 1146 3506
Fragments arranged by size:
3506 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2120 2120
Size: 5108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 381 484 556 631 826 1043 1127 1215
Size: 168 103 72 75 195 217 84 88
Cuts at: 1215 1547 1551 1606 1774 1799 1803 1971 2933
Size: 332 4 55 168 25 4 168 962
Cuts at: 2933 3365 4112 4380 213
Size: 432 747 268 941
Fragments arranged by size:
962 941 747 432 332 268 217 195
168 168 168 103 88 84 75 72
55 25 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1401 1401
Size: 5108
EcoO109I rG'GnC_Cy
Cuts at: 218 2090 2247 218
Size: 1872 157 3079
Fragments arranged by size:
3079 1872 157
EcoRI G'AATT_C
Cuts at: 1097 2112 1097
Size: 1015 4093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 5108
FspI TGC'GCA
Cuts at: 2889 2889
Size: 5108
HaeII r_GCGC'y
Cuts at: 2542 2790 3812 4791 2542
Size: 248 1022 979 2859
Fragments arranged by size:
2859 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 973 1005 1647 1650 1679 1682 1710 1742 1816
Size: 32 642 3 29 3 28 32 74
Cuts at: 1816 1848 1944 1976 2950 2980 2982 3012 973
Size: 32 96 32 974 30 2 30 3069
Fragments arranged by size:
3069 974 642 96 74 32 32 32
32 30 30 29 28 3 3 2
HincII GTy'rAC
Cuts at: 943 2147 943
Size: 1204 3904
KasI G'GCGC_C
Cuts at: 2538 2786 2538
Size: 248 4860
KpnI G_GTAC'C
Cuts at: 2128 2128
Size: 5108
MluI A'CGCG_T
Cuts at: 4541 4541
Size: 5108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3779 3963 3779
Size: 184 4924
MscI TGG'CCA
Cuts at: 607 2869 607
Size: 2262 2846
MslI CAynn'nnrTG
Cuts at: 409 997 1374 1626 3097 3224 409
Size: 588 377 252 1471 127 2293
Fragments arranged by size:
2293 1471 588 377 252 127
MspA1I CmG'CkG
Cuts at: 270 441 2219 2533 2893 3906 4151 270
Size: 171 1778 314 360 1013 245 1227
Fragments arranged by size:
1778 1227 1013 360 314 245 171
NaeI GCC'GGC
Cuts at: 3289 3289
Size: 5108
NarI GG'CG_CC
Cuts at: 2539 2787 2539
Size: 248 4860
NcoI C'CATG_G
Cuts at: 107 598 107
Size: 491 4617
NdeI CA'TA_TG
Cuts at: 1623 1623
Size: 5108
NgoMIV G'CCGG_C
Cuts at: 3287 3287
Size: 5108
NsiI A_TGCA'T
Cuts at: 1209 1881 1209
Size: 672 4436
NspI r_CATG'y
Cuts at: 408 1211 1767 1849 1883 1933 2161 2661 3568
Size: 803 556 82 34 50 228 500 907
Cuts at: 3568 408
Size: 1948
Fragments arranged by size:
1948 907 803 556 500 228 82 50
34
PciI A'CATG_T
Cuts at: 1929 3564 1929
Size: 1635 3473
PflMI CCAn_nnn'nTGG
Cuts at: 2086 2086
Size: 5108
PfoI T'CCnGG_A
Cuts at: 149 500 149
Size: 351 4757
PmeI GTTT'AAAC
Cuts at: 2107 2107
Size: 5108
PmlI CAC'GTG
Cuts at: 631 1512 631
Size: 881 4227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 811 843 4247 4279 811
Size: 32 3404 32 1640
Fragments arranged by size:
3404 1640 32 32
PpuMI rG'GwC_Cy
Cuts at: 218 218
Size: 5108
PspOMI G'GGCC_C
Cuts at: 2089 2089
Size: 5108
PstI C_TGCA'G
Cuts at: 1278 2155 2840 1278
Size: 877 685 3546
Fragments arranged by size:
3546 877 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5108
PvuII CAG'CTG
Cuts at: 270 441 2533 2893 270
Size: 171 2092 360 2485
Fragments arranged by size:
2485 2092 360 171
RsrII CG'GwC_CG
Cuts at: 3303 3303
Size: 5108
SacI G_AGCT'C
Cuts at: 2122 2122
Size: 5108
SalI G'TCGA_C
Cuts at: 2145 2145
Size: 5108
SapI GCTCTTCn'nnn_
Cuts at: 3131 3341 3131
Size: 210 4898
SbfI CC_TGCA'GG
Cuts at: 2155 2155
Size: 5108
ScaI AGT'ACT
Cuts at: 4625 4625
Size: 5108
SfcI C'TryA_G
Cuts at: 33 813 1274 2151 2836 3829 4020 33
Size: 780 461 877 685 993 191 1121
Fragments arranged by size:
1121 993 877 780 685 461 191
SfoI GGC'GCC
Cuts at: 2540 2788 2540
Size: 248 4860
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
SmaI CCC'GGG
Cuts at: 2130 2130
Size: 5108
SmlI C'TyrA_G
Cuts at: 479 1051 1734 2263 3178 3670 3932 479
Size: 572 683 529 915 492 262 1655
Fragments arranged by size:
1655 915 683 572 529 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5108
SphI G_CATG'C
Cuts at: 1883 2161 2661 1883
Size: 278 500 4330
Fragments arranged by size:
4330 500 278
SspI AAT'ATT
Cuts at: 1321 1321
Size: 5108
StyI C'CwwG_G
Cuts at: 107 511 598 2242 2596 107
Size: 404 87 1644 354 2619
Fragments arranged by size:
2619 1644 404 354 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 591 747 2691 2824 3283 3492 591
Size: 156 1944 133 459 209 2207
Fragments arranged by size:
2207 1944 459 209 156 133
TatI w'GTAC_w
Cuts at: 1926 2010 4623 1926
Size: 84 2613 2411
Fragments arranged by size:
2613 2411 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 300 332 1845 1877 4625 4657 300
Size: 32 1513 32 2748 32 751
Fragments arranged by size:
2748 1513 751 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2905 2905
Size: 5108
XbaI T'CTAG_A
Cuts at: 2139 2139
Size: 5108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 5108
XhoI C'TCGA_G
Cuts at: 479 479
Size: 5108
XmaI C'CCGG_G
Cuts at: 2128 2128
Size: 5108
XmnI GAAnn'nnTTC
Cuts at: 1396 1480 1564 1984 1396
Size: 84 84 420 4520
Fragments arranged by size:
4520 420 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BaeI
BcgI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI