Restriction map for
pF1KB6208
(Circular) (Six-base) MAPSORT of: pF1KB6208.seq Check: 6038 from: 1 to: 5273
pF1KB6208 5273 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 236 236
Size: 5273
AccI GT'mk_AC
Cuts at: 2311 2311
Size: 5273
Acc65I G'GTAC_C
Cuts at: 1163 2289 1163
Size: 1126 4147
AclI AA'CG_TT
Cuts at: 5113 5113
Size: 5273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 916 3098 3530 4277 4545 859
Size: 57 2182 432 747 268 1587
Fragments arranged by size:
2182 1587 747 432 268 57
AfeI AGC'GCT
Cuts at: 322 322
Size: 5273
AflII C'TTAA_G
Cuts at: 817 817
Size: 5273
AflIII A'CryG_T
Cuts at: 464 2625 3729 4706 464
Size: 2161 1104 977 1031
Fragments arranged by size:
2161 1104 1031 977
AgeI A'CCGG_T
Cuts at: 1166 3661 1166
Size: 2495 2778
AleI CACnn'nnGTG
Cuts at: 2053 2053
Size: 5273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 768 768
Size: 5273
AlwNI CAG_nnn'CTG
Cuts at: 601 1081 2578 4145 601
Size: 480 1497 1567 1729
Fragments arranged by size:
1729 1567 1497 480
ApaI G_GGCC'C
Cuts at: 640 640
Size: 5273
ApaLI G'TGCA_C
Cuts at: 1228 4043 1228
Size: 2815 2458
ApoI r'AATT_y
Cuts at: 839 2277 4626 4882 839
Size: 1438 2349 256 1230
Fragments arranged by size:
2349 1438 1230 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5273
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
AvaI C'yCGr_G
Cuts at: 241 251 281 2293 241
Size: 10 30 2012 3221
Fragments arranged by size:
3221 2012 30 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1155 1188 1155
Size: 33 5240
BamHI G'GATC_C
Cuts at: 2298 2298
Size: 5273
BanI G'GyrC_C
Cuts at: 1000 1163 1363 1405 1488 1727 1892 2004 2169
Size: 163 200 42 83 239 165 112 165
Cuts at: 2169 2230 2263 2289 2703 2951 2986 1000
Size: 61 33 26 414 248 35 3287
Fragments arranged by size:
3287 414 248 239 200 165 165 163
112 83 61 42 35 33 26
BanII G_rGCy'C
Cuts at: 640 811 1089 1123 1552 1812 2048 2287 3317
Size: 171 278 34 429 260 236 239 1030
Cuts at: 3317 640
Size: 2596
Fragments arranged by size:
2596 1030 429 278 260 239 236 171
34
BbeI G_GCGC'C
Cuts at: 1731 1896 2234 2707 2955 1731
Size: 165 338 473 248 4049
Fragments arranged by size:
4049 473 338 248 165
BciVI GTATCCnnnnn_n'
Cuts at: 3171 3932 5273 3171
Size: 761 1341 3171
Fragments arranged by size:
3171 1341 761
BclI T'GATC_A
Cuts at: 1047 2793 1047
Size: 1746 3527
BglI GCCn_nnn'nGGC
Cuts at: 1127 1127
Size: 5273
BglII A'GATC_T
Cuts at: 2788 4825 2788
Size: 2037 3236
BlpI GC'TnA_GC
Cuts at: 2069 2385 2069
Size: 316 4957
Bme1580I G_kGCm'C
Cuts at: 640 1003 1057 1232 1410 1603 1768 2266 2898
Size: 363 54 175 178 193 165 498 632
Cuts at: 2898 2991 4047 640
Size: 93 1056 1866
Fragments arranged by size:
1866 1056 632 498 363 193 178 175
165 93 54
BmgBI CAC'GTC
Cuts at: 465 888 465
Size: 423 4850
BmrI ACTGGGnnnn_n'
Cuts at: 538 765 1037 1848 2485 2566 2901 5045 538
Size: 227 272 811 637 81 335 2144 766
Fragments arranged by size:
2144 811 766 637 335 272 227 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 277 411 2124 3640 277
Size: 134 1713 1516 1910
Fragments arranged by size:
1910 1713 1516 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1103 541
Size: 562 4711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3328 3820 4118 2449
Size: 879 492 298 3604
Fragments arranged by size:
3604 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1367 1784 4977 1367
Size: 417 3193 1663
Fragments arranged by size:
3193 1663 417
BsaAI yAC'GTr
Cuts at: 2626 2626
Size: 5273
BsaBI GATnn'nnATC
Cuts at: 6 2787 4824 6
Size: 2781 2037 455
Fragments arranged by size:
2781 2037 455
BsaHI Gr'CG_yC
Cuts at: 233 1728 1893 2231 2704 2952 3654 5150 233
Size: 1495 165 338 473 248 702 1496 356
Fragments arranged by size:
1496 1495 702 473 356 338 248 165
BsaWI w'CCGG_w
Cuts at: 202 1166 1828 2462 2983 3661 3935 4082 4493
Size: 964 662 634 521 678 274 147 411
Cuts at: 4493 202
Size: 982
Fragments arranged by size:
982 964 678 662 634 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 427 457 1534 1564 1787 1817 427
Size: 30 1077 30 223 30 3883
Fragments arranged by size:
3883 1077 223 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 136 400 448 1300 2113 2191 127
Size: 9 264 48 852 813 78 3209
Fragments arranged by size:
3209 852 813 264 78 48 9
BseYI C'CCAG_C
Cuts at: 620 650 950 1004 1088 1131 1308 1367 1709
Size: 30 300 54 84 43 177 59 342
Cuts at: 1709 2194 2698 3456 4033 4529 620
Size: 485 504 758 577 496 1364
Fragments arranged by size:
1364 758 577 504 496 485 342 300
177 84 59 54 43 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1807 1807
Size: 5273
BsiEI CG_ry'CG
Cuts at: 104 2815 2861 4069 104
Size: 2711 46 1208 1308
Fragments arranged by size:
2711 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1232 1388 1812 2060 2287 3065 3255 4047 1232
Size: 156 424 248 227 778 190 792 2458
Fragments arranged by size:
2458 792 778 424 248 227 190 156
BsmI GAATG_Cn'
Cuts at: 904 1189 904
Size: 285 4988
BsmBI CGTCTCn'nnnn_
Cuts at: 455 455
Size: 5273
Bsp1286I G_dGCh'C
Cuts at: 640 811 1003 1057 1089 1123 1232 1388 1410
Size: 171 192 54 32 34 109 156 22
Cuts at: 1410 1552 1603 1768 1812 2048 2060 2266 2287
Size: 142 51 165 44 236 12 206 21
Cuts at: 2287 2898 2991 3065 3255 3317 4047 640
Size: 611 93 74 190 62 730 1866
Fragments arranged by size:
1866 730 611 236 206 192 190 171
165 156 142 109 93 74 62 54
51 44 34 32 22 21 12
BspEI T'CCGG_A
Cuts at: 202 1828 4493 202
Size: 1626 2665 982
Fragments arranged by size:
2665 1626 982
BspHI T'CATG_A
Cuts at: 4449 4449
Size: 5273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1646 2323 2537 2839 3220 1646
Size: 677 214 302 381 3699
Fragments arranged by size:
3699 677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3565 3619 5103 5268 50
Size: 3515 54 1484 165 55
Fragments arranged by size:
3515 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 868 3185 868
Size: 2317 2956
BsrFI r'CCGG_y
Cuts at: 1166 1451 1994 3271 3452 3661 1166
Size: 285 543 1277 181 209 2778
Fragments arranged by size:
2778 1277 543 285 209 181
BsrGI T'GTAC_A
Cuts at: 708 708
Size: 5273
BssSI C'ACGA_G
Cuts at: 1813 3544 3902 1813
Size: 1731 358 3184
Fragments arranged by size:
3184 1731 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2650 2650
Size: 5273
BstBI TT'CG_AA
Cuts at: 3634 3634
Size: 5273
BstEII G'GTnAC_C
Cuts at: 2133 2133
Size: 5273
BstXI CCAn_nnnn'nTGG
Cuts at: 862 1012 1096 862
Size: 150 84 5039
Fragments arranged by size:
5039 150 84
BstYI r'GATC_y
Cuts at: 1 759 2298 2765 2788 3123 3369 4381 4467
Size: 758 1539 467 23 335 246 1012 86
Cuts at: 4467 4479 4825 1
Size: 12 346 449
Fragments arranged by size:
1539 1012 758 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1972 2000 1972
Size: 28 5245
BtgI C'CryG_G
Cuts at: 107 424 454 1317 107
Size: 317 30 863 4063
Fragments arranged by size:
4063 863 317 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 3223 3404 3567 1456
Size: 1767 181 163 3162
Fragments arranged by size:
3162 1767 181 163
BtsI GCAGTG_nn'
Cuts at: 689 975 2516 689
Size: 286 1541 3446
Fragments arranged by size:
3446 1541 286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1623 1658 1623
Size: 35 5238
DraI TTT'AAA
Cuts at: 269 2272 269
Size: 2003 3270
DraIII CAC_nnn'GTG
Cuts at: 1262 1392 1488 1693 1262
Size: 130 96 205 4842
Fragments arranged by size:
4842 205 130 96
DrdI GACnn_nn'nnGTC
Cuts at: 2979 3837 2979
Size: 858 4415
EaeI y'GGCC_r
Cuts at: 1022 1538 1611 1860 1877 2858 3032 3423 3450
Size: 516 73 249 17 981 174 391 27
Cuts at: 3450 4795 1022
Size: 1345 1500
Fragments arranged by size:
1500 1345 981 516 391 249 174 73
27 17
EagI C'GGCC_G
Cuts at: 2858 2858
Size: 5273
EarI CTCTTCn'nnn_
Cuts at: 377 3296 3506 377
Size: 2919 210 2144
Fragments arranged by size:
2919 2144 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 3481 3791 3937 5083 133
Size: 3348 310 146 1146 323
Fragments arranged by size:
3348 1146 323 310 146
EcoICRI GAG'CTC
Cuts at: 1810 2285 1810
Size: 475 4798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 859 916 1103 3098 3530 4277 4545 541
Size: 318 57 187 1995 432 747 268 1269
Fragments arranged by size:
1995 1269 747 432 318 268 187 57
EcoO109I rG'GnC_Cy
Cuts at: 222 345 636 957 1782 1838 2412 222
Size: 123 291 321 825 56 574 3083
Fragments arranged by size:
3083 825 574 321 291 123 56
EcoRI G'AATT_C
Cuts at: 2277 2277
Size: 5273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 5273
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5273
HaeII r_GCGC'y
Cuts at: 151 324 1731 1821 1896 2234 2707 2955 3977
Size: 173 1407 90 75 338 473 248 1022
Cuts at: 3977 4956 151
Size: 979 468
Fragments arranged by size:
1407 1022 979 473 468 338 248 173
90 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 552 584 3115 3145 3147 3177 427
Size: 32 93 32 2531 30 2 30 2523
Fragments arranged by size:
2531 2523 93 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2312 2312
Size: 5273
KasI G'GCGC_C
Cuts at: 1727 1892 2230 2703 2951 1727
Size: 165 338 473 248 4049
Fragments arranged by size:
4049 473 338 248 165
KpnI G_GTAC'C
Cuts at: 1167 2293 1167
Size: 1126 4147
MluI A'CGCG_T
Cuts at: 4706 4706
Size: 5273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3944 4128 3944
Size: 184 5089
MscI TGG'CCA
Cuts at: 1024 1540 1879 3034 1024
Size: 516 339 1155 3263
Fragments arranged by size:
3263 1155 516 339
MslI CAynn'nnrTG
Cuts at: 1403 2053 3262 3389 1403
Size: 650 1209 127 3287
Fragments arranged by size:
3287 1209 650 127
MspA1I CmG'CkG
Cuts at: 378 585 676 1200 1495 1629 1682 1713 1931
Size: 207 91 524 295 134 53 31 218
Cuts at: 1931 2384 2698 3058 4071 4316 378
Size: 453 314 360 1013 245 1335
Fragments arranged by size:
1335 1013 524 453 360 314 295 245
218 207 134 91 53 31
NaeI GCC'GGC
Cuts at: 1453 3454 1453
Size: 2001 3272
NarI GG'CG_CC
Cuts at: 1728 1893 2231 2704 2952 1728
Size: 165 338 473 248 4049
Fragments arranged by size:
4049 473 338 248 165
NcoI C'CATG_G
Cuts at: 107 454 107
Size: 347 4926
NdeI CA'TA_TG
Cuts at: 1304 1565 1304
Size: 261 5012
NgoMIV G'CCGG_C
Cuts at: 1451 3452 1451
Size: 2001 3272
NspI r_CATG'y
Cuts at: 2326 2826 3733 2326
Size: 500 907 3866
Fragments arranged by size:
3866 907 500
PasI CC'CwG_GG
Cuts at: 730 730
Size: 5273
PciI A'CATG_T
Cuts at: 3729 3729
Size: 5273
PflMI CCAn_nnn'nTGG
Cuts at: 1081 1308 1081
Size: 227 5046
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 4412
Size: 32 5241
PpuMI rG'GwC_Cy
Cuts at: 345 957 345
Size: 612 4661
PspOMI G'GGCC_C
Cuts at: 636 636
Size: 5273
PstI C_TGCA'G
Cuts at: 356 2014 2066 2320 3005 356
Size: 1658 52 254 685 2624
Fragments arranged by size:
2624 1658 685 254 52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5273
PvuII CAG'CTG
Cuts at: 585 676 1200 1495 1713 2698 3058 585
Size: 91 524 295 218 985 360 2800
Fragments arranged by size:
2800 985 524 360 295 218 91
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5273
SacI G_AGCT'C
Cuts at: 1812 2287 1812
Size: 475 4798
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5273
SanDI GG'GwC_CC
Cuts at: 345 345
Size: 5273
SapI GCTCTTCn'nnn_
Cuts at: 3296 3506 3296
Size: 210 5063
SbfI CC_TGCA'GG
Cuts at: 2066 2320 2066
Size: 254 5019
ScaI AGT'ACT
Cuts at: 4790 4790
Size: 5273
SexAI A'CCwGG_T
Cuts at: 1388 1484 1388
Size: 96 5177
SfcI C'TryA_G
Cuts at: 33 352 677 1195 1275 2010 2062 2316 3001
Size: 319 325 518 80 735 52 254 685
Cuts at: 3001 3994 4185 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 735 685 518 325 319 254
191 80 52
SfoI GGC'GCC
Cuts at: 1729 1894 2232 2705 2953 1729
Size: 165 338 473 248 4049
Fragments arranged by size:
4049 473 338 248 165
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
SmaI CCC'GGG
Cuts at: 283 2295 283
Size: 2012 3261
SmlI C'TyrA_G
Cuts at: 817 2428 3343 3835 4097 817
Size: 1611 915 492 262 1993
Fragments arranged by size:
1993 1611 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5273
SphI G_CATG'C
Cuts at: 2326 2826 2326
Size: 500 4773
StuI AGG'CCT
Cuts at: 1357 1441 1357
Size: 84 5189
StyI C'CwwG_G
Cuts at: 107 294 311 398 454 845 2407 2761 107
Size: 187 17 87 56 391 1562 354 2619
Fragments arranged by size:
2619 1562 391 354 187 87 56 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 851 1018 1074 1381 2856 2989 3448 3657
Size: 187 167 56 307 1475 133 459 209
Cuts at: 3657 664
Size: 2280
Fragments arranged by size:
2280 1475 459 307 209 187 167 133
56
TatI w'GTAC_w
Cuts at: 708 4788 708
Size: 4080 1193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1787 1819 4790 4822 1787
Size: 32 2971 32 2238
Fragments arranged by size:
2971 2238 32 32
Tth111I GACn'n_nGTC
Cuts at: 3070 3070
Size: 5273
XbaI T'CTAG_A
Cuts at: 2304 2304
Size: 5273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 778 1944 2768 778
Size: 1166 824 3283
Fragments arranged by size:
3283 1166 824
XmaI C'CCGG_G
Cuts at: 281 2293 281
Size: 2012 3261
ZraI GAC'GTC
Cuts at: 234 234
Size: 5273
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AhdI AloI AscI AvrII BbsI BbvCI BcgI
BfrBI BmtI Bpu10I BsiWI BssHII BstZ17I ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI