Restriction map for
pF1KB6212
(Circular) (Six-base) MAPSORT of: pF1KB6212.seq Check: 2092 from: 1 to: 5207
pF1KB6212 5207 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 276 276
Size: 5207
AatII G_ACGT'C
Cuts at: 1130 1130
Size: 5207
AccI GT'mk_AC
Cuts at: 350 2245 350
Size: 1895 3312
Acc65I G'GTAC_C
Cuts at: 276 932 2223 276
Size: 656 1291 3260
Fragments arranged by size:
3260 1291 656
AclI AA'CG_TT
Cuts at: 5047 5047
Size: 5207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1840 3032 3464 4211 4479 868
Size: 972 1192 432 747 268 1596
Fragments arranged by size:
1596 1192 972 747 432 268
AfeI AGC'GCT
Cuts at: 1926 1926
Size: 5207
AflIII A'CryG_T
Cuts at: 305 1574 1622 1976 2559 3663 4640 305
Size: 1269 48 354 583 1104 977 872
Fragments arranged by size:
1269 1104 977 872 583 354 48
AgeI A'CCGG_T
Cuts at: 3595 3595
Size: 5207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 5207
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 733 765 733
Size: 32 5175
AlwNI CAG_nnn'CTG
Cuts at: 639 1392 1563 1731 1818 2512 4079 639
Size: 753 171 168 87 694 1567 1767
Fragments arranged by size:
1767 1567 753 694 171 168 87
ApaI G_GGCC'C
Cuts at: 240 2128 240
Size: 1888 3319
ApaLI G'TGCA_C
Cuts at: 3977 3977
Size: 5207
ApoI r'AATT_y
Cuts at: 114 2211 4560 4816 114
Size: 2097 2349 256 505
Fragments arranged by size:
2349 2097 505 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5207
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5207
AvaI C'yCGr_G
Cuts at: 391 961 1066 1291 1604 2227 391
Size: 570 105 225 313 623 3371
Fragments arranged by size:
3371 623 570 313 225 105
BamHI G'GATC_C
Cuts at: 2232 2232
Size: 5207
BanI G'GyrC_C
Cuts at: 276 448 805 932 1297 2223 2637 2885 2920
Size: 172 357 127 365 926 414 248 35
Cuts at: 2920 276
Size: 2563
Fragments arranged by size:
2563 926 414 365 357 248 172 127
35
BanII G_rGCy'C
Cuts at: 240 535 1771 2128 2153 2221 3251 240
Size: 295 1236 357 25 68 1030 2196
Fragments arranged by size:
2196 1236 1030 357 295 68 25
BbeI G_GCGC'C
Cuts at: 1301 2641 2889 1301
Size: 1340 248 3619
Fragments arranged by size:
3619 1340 248
BbsI GAAGACnn'nnnn_
Cuts at: 955 1053 1292 955
Size: 98 239 4870
Fragments arranged by size:
4870 239 98
BbvCI CC'TCA_GC
Cuts at: 1420 1420
Size: 5207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5173
BciVI GTATCCnnnnn_n'
Cuts at: 320 3105 3866 5207 320
Size: 2785 761 1341 320
Fragments arranged by size:
2785 1341 761 320
BclI T'GATC_A
Cuts at: 2727 2727
Size: 5207
BglII A'GATC_T
Cuts at: 2722 4759 2722
Size: 2037 3170
BlpI GC'TnA_GC
Cuts at: 1645 2319 1645
Size: 674 4533
Bme1580I G_kGCm'C
Cuts at: 240 453 808 2128 2832 2925 3981 240
Size: 213 355 1320 704 93 1056 1466
Fragments arranged by size:
1466 1320 1056 704 355 213 93
BmgBI CAC'GTC
Cuts at: 1059 1416 1577 1625 1059
Size: 357 161 48 4641
Fragments arranged by size:
4641 357 161 48
BmrI ACTGGGnnnn_n'
Cuts at: 730 1427 2419 2500 2835 4979 730
Size: 697 992 81 335 2144 958
Fragments arranged by size:
2144 992 958 697 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 1707 3574 577
Size: 1130 1867 2210
Fragments arranged by size:
2210 1867 1130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 616 1177 1233 1753 528
Size: 88 561 56 520 3982
Fragments arranged by size:
3982 561 520 88 56
Bpu10I CC'TnA_GC
Cuts at: 1420 1420
Size: 5207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2383 3262 3754 4052 2383
Size: 879 492 298 3538
Fragments arranged by size:
3538 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4911 4911
Size: 5207
BsaAI yAC'GTr
Cuts at: 306 330 2560 306
Size: 24 2230 2953
Fragments arranged by size:
2953 2230 24
BsaBI GATnn'nnATC
Cuts at: 6 1405 2721 4758 6
Size: 1399 1316 2037 455
Fragments arranged by size:
2037 1399 1316 455
BsaHI Gr'CG_yC
Cuts at: 344 1112 1127 1298 1538 2023 2132 2638 2886
Size: 768 15 171 240 485 109 506 248
Cuts at: 2886 3588 5084 344
Size: 702 1496 467
Fragments arranged by size:
1496 768 702 506 485 467 248 240
171 109 15
BsaWI w'CCGG_w
Cuts at: 999 2014 2396 2917 3595 3869 4016 4427 999
Size: 1015 382 521 678 274 147 411 1779
Fragments arranged by size:
1779 1015 678 521 411 382 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 332 362 733 763 958 988 332
Size: 30 371 30 195 30 4551
Fragments arranged by size:
4551 371 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1236 1377 1726 1236
Size: 141 349 4717
Fragments arranged by size:
4717 349 141
BseYI C'CCAG_C
Cuts at: 281 412 801 1074 1840 2632 3390 3967 4463
Size: 131 389 273 766 792 758 577 496
Cuts at: 4463 281
Size: 1025
Fragments arranged by size:
1025 792 766 758 577 496 389 273
131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 319 343 973 1039 1105 1699 1744 310
Size: 9 24 630 66 66 594 45 3773
Fragments arranged by size:
3773 630 594 66 66 45 24 9
BsiEI CG_ry'CG
Cuts at: 104 2030 2749 2795 4003 104
Size: 1926 719 46 1208 1308
Fragments arranged by size:
1926 1308 1208 719 46
BsiHKAI G_wGCw'C
Cuts at: 725 1771 1997 2221 2999 3189 3981 725
Size: 1046 226 224 778 190 792 1951
Fragments arranged by size:
1951 1046 792 778 226 224 190
BsmBI CGTCTCn'nnnn_
Cuts at: 335 546 2014 335
Size: 211 1468 3528
Fragments arranged by size:
3528 1468 211
Bsp1286I G_dGCh'C
Cuts at: 240 453 535 725 808 1771 1997 2128 2153
Size: 213 82 190 83 963 226 131 25
Cuts at: 2153 2221 2832 2925 2999 3189 3251 3981 240
Size: 68 611 93 74 190 62 730 1466
Fragments arranged by size:
1466 963 730 611 226 213 190 190
131 93 83 82 74 68 62 25
BspEI T'CCGG_A
Cuts at: 999 4427 999
Size: 3428 1779
BspHI T'CATG_A
Cuts at: 4383 4383
Size: 5207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 276 2257 2471 2773 3154 264
Size: 12 1981 214 302 381 2317
Fragments arranged by size:
2317 1981 381 302 214 12
BsrBI CCG'CTC
Cuts at: 50 130 614 3499 3553 5037 5202 50
Size: 80 484 2885 54 1484 165 55
Fragments arranged by size:
2885 1484 484 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 3119 3119
Size: 5207
BsrFI r'CCGG_y
Cuts at: 294 869 935 1425 1831 3205 3386 3595 294
Size: 575 66 490 406 1374 181 209 1906
Fragments arranged by size:
1906 1374 575 490 406 209 181 66
BsrGI T'GTAC_A
Cuts at: 1148 1148
Size: 5207
BssSI C'ACGA_G
Cuts at: 1692 3478 3836 1692
Size: 1786 358 3063
Fragments arranged by size:
3063 1786 358
BstAPI GCAn_nnn'nTGC
Cuts at: 297 915 1869 2584 297
Size: 618 954 715 2920
Fragments arranged by size:
2920 954 715 618
BstBI TT'CG_AA
Cuts at: 3568 3568
Size: 5207
BstEII G'GTnAC_C
Cuts at: 456 1806 456
Size: 1350 3857
BstXI CCAn_nnnn'nTGG
Cuts at: 835 1473 1794 835
Size: 638 321 4248
Fragments arranged by size:
4248 638 321
BstYI r'GATC_y
Cuts at: 1 2232 2699 2722 3057 3303 4315 4401 4413
Size: 2231 467 23 335 246 1012 86 12
Cuts at: 4413 4759 1
Size: 346 449
Fragments arranged by size:
2231 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1351 1351
Size: 5207
BtgI C'CryG_G
Cuts at: 146 461 515 567 1010 1895 146
Size: 315 54 52 443 885 3458
Fragments arranged by size:
3458 885 443 315 54 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 602 2114 3157 3338 3501 602
Size: 1512 1043 181 163 2308
Fragments arranged by size:
2308 1512 1043 181 163
BtsI GCAGTG_nn'
Cuts at: 711 1117 1158 2178 2450 711
Size: 406 41 1020 272 3468
Fragments arranged by size:
3468 1020 406 272 41
DraI TTT'AAA
Cuts at: 2206 2206
Size: 5207
DrdI GACnn_nn'nnGTC
Cuts at: 1804 2913 3771 1804
Size: 1109 858 3240
Fragments arranged by size:
3240 1109 858
EaeI y'GGCC_r
Cuts at: 896 937 1013 2792 2966 3357 3384 4729 896
Size: 41 76 1779 174 391 27 1345 1374
Fragments arranged by size:
1779 1374 1345 391 174 76 41 27
EagI C'GGCC_G
Cuts at: 2792 2792
Size: 5207
EarI CTCTTCn'nnn_
Cuts at: 1274 3230 3440 1274
Size: 1956 210 3041
Fragments arranged by size:
3041 1956 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1945 3415 3725 3871 5017 1945
Size: 1470 310 146 1146 2135
Fragments arranged by size:
2135 1470 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1769 2219 1769
Size: 450 4757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 616 868 1177 1233 1753 1840 3032 3464
Size: 88 252 309 56 520 87 1192 432
Cuts at: 3464 4211 4479 528
Size: 747 268 1256
Fragments arranged by size:
1256 1192 747 520 432 309 268 252
88 87 56
EcoNI CCTnn'n_nnAGG
Cuts at: 1102 1309 1384 1594 1102
Size: 207 75 210 4715
Fragments arranged by size:
4715 210 207 75
EcoO109I rG'GnC_Cy
Cuts at: 236 438 1134 1815 1908 2346 236
Size: 202 696 681 93 438 3097
Fragments arranged by size:
3097 696 681 438 202 93
EcoRI G'AATT_C
Cuts at: 114 2211 114
Size: 2097 3110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 5207
FspI TGC'GCA
Cuts at: 1921 2988 1921
Size: 1067 4140
HaeII r_GCGC'y
Cuts at: 1081 1301 1393 1572 1928 2641 2889 3911 4890
Size: 220 92 179 356 713 248 1022 979
Cuts at: 4890 1081
Size: 1398
Fragments arranged by size:
1398 1022 979 713 356 248 220 179
92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 332 364 958 990 2044 2076 3049 3079 3081
Size: 32 594 32 1054 32 973 30 2
Cuts at: 3081 3111 332
Size: 30 2428
Fragments arranged by size:
2428 1054 973 594 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1487 2246 1487
Size: 759 4448
HindIII A'AGCT_T
Cuts at: 1318 1318
Size: 5207
KasI G'GCGC_C
Cuts at: 1297 2637 2885 1297
Size: 1340 248 3619
Fragments arranged by size:
3619 1340 248
KpnI G_GTAC'C
Cuts at: 280 936 2227 280
Size: 656 1291 3260
Fragments arranged by size:
3260 1291 656
MluI A'CGCG_T
Cuts at: 4640 4640
Size: 5207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 3878 4062 623
Size: 3255 184 1768
Fragments arranged by size:
3255 1768 184
MscI TGG'CCA
Cuts at: 898 2968 898
Size: 2070 3137
MslI CAynn'nnrTG
Cuts at: 514 833 898 1015 3196 3323 514
Size: 319 65 117 2181 127 2398
Fragments arranged by size:
2398 2181 319 127 117 65
MspA1I CmG'CkG
Cuts at: 484 661 971 983 1143 1728 1790 2318 2632
Size: 177 310 12 160 585 62 528 314
Cuts at: 2632 2992 4005 4250 484
Size: 360 1013 245 1441
Fragments arranged by size:
1441 1013 585 528 360 314 310 245
177 160 62 12
NaeI GCC'GGC
Cuts at: 1833 3388 1833
Size: 1555 3652
NarI GG'CG_CC
Cuts at: 1298 2638 2886 1298
Size: 1340 248 3619
Fragments arranged by size:
3619 1340 248
NcoI C'CATG_G
Cuts at: 515 1010 1895 515
Size: 495 885 3827
Fragments arranged by size:
3827 885 495
NgoMIV G'CCGG_C
Cuts at: 1831 3386 1831
Size: 1555 3652
NspI r_CATG'y
Cuts at: 1831 1980 2260 2760 3667 1831
Size: 149 280 500 907 3371
Fragments arranged by size:
3371 907 500 280 149
PciI A'CATG_T
Cuts at: 1976 3663 1976
Size: 1687 3520
PflMI CCAn_nnn'nTGG
Cuts at: 501 1392 501
Size: 891 4316
PfoI T'CCnGG_A
Cuts at: 1904 1904
Size: 5207
PmeI GTTT'AAAC
Cuts at: 2206 2206
Size: 5207
PmlI CAC'GTG
Cuts at: 306 306
Size: 5207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 733 765 1963 1995 4346 4378 733
Size: 32 1198 32 2351 32 1562
Fragments arranged by size:
2351 1562 1198 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1815 1908 1815
Size: 93 5114
PspOMI G'GGCC_C
Cuts at: 236 2124 236
Size: 1888 3319
PstI C_TGCA'G
Cuts at: 878 1106 1142 1619 1760 2195 2254 2939 878
Size: 228 36 477 141 435 59 685 3146
Fragments arranged by size:
3146 685 477 435 228 141 59 36
PvuI CG_AT'CG
Cuts at: 104 2030 104
Size: 1926 3281
PvuII CAG'CTG
Cuts at: 1143 1790 2632 2992 1143
Size: 647 842 360 3358
Fragments arranged by size:
3358 842 647 360
RsrII CG'GwC_CG
Cuts at: 3402 3402
Size: 5207
SacI G_AGCT'C
Cuts at: 1771 2221 1771
Size: 450 4757
SalI G'TCGA_C
Cuts at: 2244 2244
Size: 5207
SapI GCTCTTCn'nnn_
Cuts at: 3230 3440 3230
Size: 210 4997
SbfI CC_TGCA'GG
Cuts at: 2195 2254 2195
Size: 59 5148
ScaI AGT'ACT
Cuts at: 4724 4724
Size: 5207
SexAI A'CCwGG_T
Cuts at: 1985 1985
Size: 5207
SfcI C'TryA_G
Cuts at: 33 874 1102 1138 1172 1615 1756 2191 2250
Size: 841 228 36 34 443 141 435 59
Cuts at: 2250 2935 3928 4119 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 841 685 443 435 228 191
141 59 36 34
SfoI GGC'GCC
Cuts at: 1299 2639 2887 1299
Size: 1340 248 3619
Fragments arranged by size:
3619 1340 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5207
SmaI CCC'GGG
Cuts at: 1606 2229 1606
Size: 623 4584
SmlI C'TyrA_G
Cuts at: 2362 3277 3769 4031 2362
Size: 915 492 262 3538
Fragments arranged by size:
3538 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5207
SphI G_CATG'C
Cuts at: 1831 2260 2760 1831
Size: 429 500 4278
Fragments arranged by size:
4278 500 429
SrfI GCCC'GGGC
Cuts at: 1606 1606
Size: 5207
StuI AGG'CCT
Cuts at: 1243 1690 1243
Size: 447 4760
StyI C'CwwG_G
Cuts at: 515 1010 1310 1529 1850 1895 2341 2695 515
Size: 495 300 219 321 45 446 354 3027
Fragments arranged by size:
3027 495 446 354 321 300 219 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1825 2134 2790 2923 3382 3591 1686
Size: 139 309 656 133 459 209 3302
Fragments arranged by size:
3302 656 459 309 209 139 133
TatI w'GTAC_w
Cuts at: 890 1040 1148 1973 4722 890
Size: 150 108 825 2749 1375
Fragments arranged by size:
2749 1375 825 150 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1254 1286 1436 1468 4724 4756 1254
Size: 32 150 32 3256 32 1705
Fragments arranged by size:
3256 1705 150 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 346 1111 3004 346
Size: 765 1893 2549
Fragments arranged by size:
2549 1893 765
XbaI T'CTAG_A
Cuts at: 2238 2238
Size: 5207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 594 2702 594
Size: 2108 3099
XmaI C'CCGG_G
Cuts at: 1604 2227 1604
Size: 623 4584
ZraI GAC'GTC
Cuts at: 1128 1128
Size: 5207
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI AvrII BaeI BfrBI BglI
BmtI BsiWI BsmI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SspI SwaI XhoI
XmnI