Restriction map for
pF1KB6213
(Circular) (Six-base) MAPSORT of: pF1KB6213.seq Check: 270 from: 1 to: 5117
pF1KB6213 5117 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 856 1109 2155 856
Size: 253 1046 3818
Fragments arranged by size:
3818 1046 253
Acc65I G'GTAC_C
Cuts at: 864 1717 2133 864
Size: 853 416 3848
Fragments arranged by size:
3848 853 416
AclI AA'CG_TT
Cuts at: 4957 4957
Size: 5117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1103 1339 1415 1459 1832 2035 2942 3374
Size: 727 236 76 44 373 203 907 432
Cuts at: 3374 4121 4389 376
Size: 747 268 1104
Fragments arranged by size:
1104 907 747 727 432 373 268 236
203 76 44
AflIII A'CryG_T
Cuts at: 119 1031 2469 3573 4550 119
Size: 912 1438 1104 977 686
Fragments arranged by size:
1438 1104 977 912 686
AgeI A'CCGG_T
Cuts at: 1049 3505 1049
Size: 2456 2661
AleI CACnn'nnGTG
Cuts at: 767 767
Size: 5117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 785 785
Size: 5117
AlwNI CAG_nnn'CTG
Cuts at: 468 778 2422 3989 468
Size: 310 1644 1567 1596
Fragments arranged by size:
1644 1596 1567 310
ApaI G_GGCC'C
Cuts at: 1255 1953 1255
Size: 698 4419
ApaLI G'TGCA_C
Cuts at: 3887 3887
Size: 5117
ApoI r'AATT_y
Cuts at: 2121 4470 4726 2121
Size: 2349 256 2512
Fragments arranged by size:
2512 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5117
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
AvaI C'yCGr_G
Cuts at: 698 785 1349 1712 2137 698
Size: 87 564 363 425 3678
Fragments arranged by size:
3678 564 425 363 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 848 881 848
Size: 33 5084
BamHI G'GATC_C
Cuts at: 2142 2142
Size: 5117
BanI G'GyrC_C
Cuts at: 864 1354 1689 1717 2133 2547 2795 2830 864
Size: 490 335 28 416 414 248 35 3151
Fragments arranged by size:
3151 490 416 414 335 248 35 28
BanII G_rGCy'C
Cuts at: 197 726 1255 1953 2131 3161 197
Size: 529 529 698 178 1030 2153
Fragments arranged by size:
2153 1030 698 529 529 178
BbeI G_GCGC'C
Cuts at: 2551 2799 2551
Size: 248 4869
BbsI GAAGACnn'nnnn_
Cuts at: 526 1327 526
Size: 801 4316
BciVI GTATCCnnnnn_n'
Cuts at: 807 1935 3015 3776 5117 807
Size: 1128 1080 761 1341 807
Fragments arranged by size:
1341 1128 1080 807 761
BclI T'GATC_A
Cuts at: 534 2637 534
Size: 2103 3014
BglI GCCn_nnn'nGGC
Cuts at: 112 922 1435 112
Size: 810 513 3794
Fragments arranged by size:
3794 810 513
BglII A'GATC_T
Cuts at: 1674 2632 4669 1674
Size: 958 2037 2122
Fragments arranged by size:
2122 2037 958
BlpI GC'TnA_GC
Cuts at: 2229 2229
Size: 5117
Bme1580I G_kGCm'C
Cuts at: 1255 1357 1953 2742 2835 3891 1255
Size: 102 596 789 93 1056 2481
Fragments arranged by size:
2481 1056 789 596 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 1774 2329 2410 2745 4889 1774
Size: 555 81 335 2144 2002
Fragments arranged by size:
2144 2002 555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1386 3484 1386
Size: 2098 3019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 2125
Size: 5117
Bpu10I CC'TnA_GC
Cuts at: 845 845
Size: 5117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 1356 1357 2293 3172 3664 3962 477
Size: 879 1 936 879 492 298 1632
Fragments arranged by size:
1632 936 879 879 492 298 1
BsaI GGTCTCn'nnnn_
Cuts at: 4821 4821
Size: 5117
BsaAI yAC'GTr
Cuts at: 2470 2470
Size: 5117
BsaBI GATnn'nnATC
Cuts at: 6 2631 4668 6
Size: 2625 2037 455
Fragments arranged by size:
2625 2037 455
BsaHI Gr'CG_yC
Cuts at: 2548 2796 3498 4994 2548
Size: 248 702 1496 2671
Fragments arranged by size:
2671 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1049 2306 2827 3505 3779 3926 4337 1049
Size: 1257 521 678 274 147 411 1829
Fragments arranged by size:
1829 1257 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 125 155 866 895 896 925 125
Size: 30 711 29 1 29 4317
Fragments arranged by size:
4317 711 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 952 205
Size: 747 4370
BseYI C'CCAG_C
Cuts at: 1899 2542 3300 3877 4373 1899
Size: 643 758 577 496 2643
Fragments arranged by size:
2643 758 643 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 791 1533 2048 535
Size: 256 742 515 3604
Fragments arranged by size:
3604 742 515 256
BsiEI CG_ry'CG
Cuts at: 104 2659 2705 3913 104
Size: 2555 46 1208 1308
Fragments arranged by size:
2555 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 197 1594 2131 2909 3099 3891 197
Size: 1397 537 778 190 792 1423
Fragments arranged by size:
1423 1397 792 778 537 190
Bsp1286I G_dGCh'C
Cuts at: 197 726 1255 1357 1594 1953 2131 2742 2835
Size: 529 529 102 237 359 178 611 93
Cuts at: 2835 2909 3099 3161 3891 197
Size: 74 190 62 730 1423
Fragments arranged by size:
1423 730 611 529 529 359 237 190
178 102 93 74 62
BspEI T'CCGG_A
Cuts at: 4337 4337
Size: 5117
BspHI T'CATG_A
Cuts at: 4293 4293
Size: 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2070 2167 2381 2683 3064 2070
Size: 97 214 302 381 4123
Fragments arranged by size:
4123 381 302 214 97
BsrBI CCG'CTC
Cuts at: 50 3409 3463 4947 5112 50
Size: 3359 54 1484 165 55
Fragments arranged by size:
3359 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3029 3029
Size: 5117
BsrFI r'CCGG_y
Cuts at: 668 1049 3115 3296 3505 668
Size: 381 2066 181 209 2280
Fragments arranged by size:
2280 2066 381 209 181
BssSI C'ACGA_G
Cuts at: 3388 3746 3388
Size: 358 4759
BstAPI GCAn_nnn'nTGC
Cuts at: 778 2494 778
Size: 1716 3401
BstBI TT'CG_AA
Cuts at: 3478 3478
Size: 5117
BstXI CCAn_nnnn'nTGG
Cuts at: 1446 1920 1446
Size: 474 4643
BstYI r'GATC_y
Cuts at: 1 575 1292 1674 2142 2609 2632 2967 3213
Size: 574 717 382 468 467 23 335 246
Cuts at: 3213 4225 4311 4323 4669 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 717 574 468 467 449 382 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1607 1607
Size: 5117
BtgI C'CryG_G
Cuts at: 107 809 1358 107
Size: 702 549 3866
Fragments arranged by size:
3866 702 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3067 3248 3411 3067
Size: 181 163 4773
Fragments arranged by size:
4773 181 163
BtsI GCAGTG_nn'
Cuts at: 432 1273 2045 2360 432
Size: 841 772 315 3189
Fragments arranged by size:
3189 841 772 315
DraI TTT'AAA
Cuts at: 2116 2116
Size: 5117
DrdI GACnn_nn'nnGTC
Cuts at: 2823 3681 2823
Size: 858 4259
EaeI y'GGCC_r
Cuts at: 113 1931 2702 2876 3267 3294 4639 113
Size: 1818 771 174 391 27 1345 591
Fragments arranged by size:
1818 1345 771 591 391 174 27
EagI C'GGCC_G
Cuts at: 2702 2702
Size: 5117
EarI CTCTTCn'nnn_
Cuts at: 162 302 3140 3350 162
Size: 140 2838 210 1929
Fragments arranged by size:
2838 1929 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3325 3635 3781 4927 3325
Size: 310 146 1146 3515
Fragments arranged by size:
3515 1146 310 146
EcoICRI GAG'CTC
Cuts at: 195 2129 195
Size: 1934 3183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1103 1339 1415 1459 1832 2035 2125 2942
Size: 727 236 76 44 373 203 90 817
Cuts at: 2942 3374 4121 4389 376
Size: 432 747 268 1104
Fragments arranged by size:
1104 817 747 727 432 373 268 236
203 90 76 44
EcoNI CCTnn'n_nnAGG
Cuts at: 472 592 822 844 1957 472
Size: 120 230 22 1113 3632
Fragments arranged by size:
3632 1113 230 120 22
EcoO109I rG'GnC_Cy
Cuts at: 645 942 1197 1251 1518 1949 1950 2256 645
Size: 297 255 54 267 431 1 306 3506
Fragments arranged by size:
3506 431 306 297 267 255 54 1
EcoRI G'AATT_C
Cuts at: 2121 2121
Size: 5117
EcoRV GAT'ATC
Cuts at: 453 792 453
Size: 339 4778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 5117
FspI TGC'GCA
Cuts at: 2898 2898
Size: 5117
HaeII r_GCGC'y
Cuts at: 348 1430 2551 2799 3821 4800 348
Size: 1082 1121 248 1022 979 665
Fragments arranged by size:
1121 1082 1022 979 665 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 567 586 599 618 895 927 1314
Size: 32 410 19 13 19 277 32 387
Cuts at: 1314 1346 1623 1655 2959 2989 2991 3021 125
Size: 32 277 32 1304 30 2 30 2221
Fragments arranged by size:
2221 1304 410 387 277 277 32 32
32 32 30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 857 2156 857
Size: 1299 3818
HindIII A'AGCT_T
Cuts at: 756 756
Size: 5117
KasI G'GCGC_C
Cuts at: 2547 2795 2547
Size: 248 4869
KpnI G_GTAC'C
Cuts at: 868 1721 2137 868
Size: 853 416 3848
Fragments arranged by size:
3848 853 416
MluI A'CGCG_T
Cuts at: 4550 4550
Size: 5117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1270 3788 3972 1270
Size: 2518 184 2415
Fragments arranged by size:
2518 2415 184
MscI TGG'CCA
Cuts at: 1933 2878 1933
Size: 945 4172
MslI CAynn'nnrTG
Cuts at: 767 1153 1777 3106 3233 767
Size: 386 624 1329 127 2651
Fragments arranged by size:
2651 1329 624 386 127
MspA1I CmG'CkG
Cuts at: 775 1746 1903 2228 2542 2902 3915 4160 775
Size: 971 157 325 314 360 1013 245 1732
Fragments arranged by size:
1732 1013 971 360 325 314 245 157
NaeI GCC'GGC
Cuts at: 3298 3298
Size: 5117
NarI GG'CG_CC
Cuts at: 2548 2796 2548
Size: 248 4869
NcoI C'CATG_G
Cuts at: 107 809 107
Size: 702 4415
NgoMIV G'CCGG_C
Cuts at: 3296 3296
Size: 5117
NspI r_CATG'y
Cuts at: 123 1035 1266 1512 2170 2670 3577 123
Size: 912 231 246 658 500 907 1663
Fragments arranged by size:
1663 912 907 658 500 246 231
PasI CC'CwG_GG
Cuts at: 870 870
Size: 5117
PciI A'CATG_T
Cuts at: 119 1031 3573 119
Size: 912 2542 1663
Fragments arranged by size:
2542 1663 912
PflMI CCAn_nnn'nTGG
Cuts at: 468 468
Size: 5117
PfoI T'CCnGG_A
Cuts at: 2102 2102
Size: 5117
PmeI GTTT'AAAC
Cuts at: 2116 2116
Size: 5117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 4256
Size: 32 5085
PpuMI rG'GwC_Cy
Cuts at: 645 1518 645
Size: 873 4244
PspOMI G'GGCC_C
Cuts at: 1251 1949 1251
Size: 698 4419
PstI C_TGCA'G
Cuts at: 2164 2849 2164
Size: 685 4432
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5117
PvuII CAG'CTG
Cuts at: 775 1746 1903 2542 2902 775
Size: 971 157 639 360 2990
Fragments arranged by size:
2990 971 639 360 157
RsrII CG'GwC_CG
Cuts at: 3312 3312
Size: 5117
SacI G_AGCT'C
Cuts at: 197 2131 197
Size: 1934 3183
SalI G'TCGA_C
Cuts at: 855 2154 855
Size: 1299 3818
SanDI GG'GwC_CC
Cuts at: 645 645
Size: 5117
SapI GCTCTTCn'nnn_
Cuts at: 3140 3350 3140
Size: 210 4907
SbfI CC_TGCA'GG
Cuts at: 2164 2164
Size: 5117
ScaI AGT'ACT
Cuts at: 4634 4634
Size: 5117
SexAI A'CCwGG_T
Cuts at: 173 173
Size: 5117
SfcI C'TryA_G
Cuts at: 33 2160 2845 3838 4029 33
Size: 2127 685 993 191 1121
Fragments arranged by size:
2127 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2549 2797 2549
Size: 248 4869
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
SmaI CCC'GGG
Cuts at: 2139 2139
Size: 5117
SmlI C'TyrA_G
Cuts at: 456 1336 1371 2272 3187 3679 3941 456
Size: 880 35 901 915 492 262 1632
Fragments arranged by size:
1632 915 901 880 492 262 35
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5117
SphI G_CATG'C
Cuts at: 2170 2670 2170
Size: 500 4617
StuI AGG'CCT
Cuts at: 1839 1839
Size: 5117
StyI C'CwwG_G
Cuts at: 107 809 1531 2251 2605 107
Size: 702 722 720 354 2619
Fragments arranged by size:
2619 722 720 702 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 2700 2833 3292 3501 421
Size: 2279 133 459 209 2037
Fragments arranged by size:
2279 2037 459 209 133
TatI w'GTAC_w
Cuts at: 4632 4632
Size: 5117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 411 443 866 898 4634 4666 411
Size: 32 423 32 3736 32 862
Fragments arranged by size:
3736 862 423 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2914 2914
Size: 5117
XbaI T'CTAG_A
Cuts at: 2148 2148
Size: 5117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 858 2612 858
Size: 1754 3363
XmaI C'CCGG_G
Cuts at: 2137 2137
Size: 5117
XmnI GAAnn'nnTTC
Cuts at: 371 964 371
Size: 593 4524
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI