(Circular) (Six-base) MAPSORT of: pF1KB6215.seq Check: 1964 from: 1 to: 5120 pF1KB6215 5120 bp With 182 enzymes: SgfI * October 7, 2009 14:20 .. AccI GT'mk_AC Cuts at: 2158 2158 Size: 5120 Acc65I G'GTAC_C Cuts at: 578 1018 1604 2136 578 Size: 440 586 532 3562 Fragments arranged by size: 3562 586 532 440 AclI AA'CG_TT Cuts at: 4960 4960 Size: 5120 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2945 3377 4124 4392 2945 Size: 432 747 268 3673 Fragments arranged by size: 3673 747 432 268 AfeI AGC'GCT Cuts at: 370 499 2095 370 Size: 129 1596 3395 Fragments arranged by size: 3395 1596 129 AflIII A'CryG_T Cuts at: 2073 2472 3576 4553 2073 Size: 399 1104 977 2640 Fragments arranged by size: 2640 1104 977 399 AgeI A'CCGG_T Cuts at: 391 751 3508 391 Size: 360 2757 2003 Fragments arranged by size: 2757 2003 360 AleI CACnn'nnGTG Cuts at: 394 1850 394 Size: 1456 3664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 865 865 Size: 5120 AlwNI CAG_nnn'CTG Cuts at: 1467 1565 1625 2425 3992 1467 Size: 98 60 800 1567 2595 Fragments arranged by size: 2595 1567 800 98 60 ApaI G_GGCC'C Cuts at: 1162 1162 Size: 5120 ApaLI G'TGCA_C Cuts at: 3890 3890 Size: 5120 ApoI r'AATT_y Cuts at: 1714 2124 4473 4729 1714 Size: 410 2349 256 2105 Fragments arranged by size: 2349 2105 410 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5120 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5120 AvaI C'yCGr_G Cuts at: 120 982 1175 1861 2140 120 Size: 862 193 686 279 3100 Fragments arranged by size: 3100 862 686 279 193 AvrII C'CTAG_G Cuts at: 792 792 Size: 5120 BamHI G'GATC_C Cuts at: 380 1634 2145 380 Size: 1254 511 3355 Fragments arranged by size: 3355 1254 511 BanI G'GyrC_C Cuts at: 444 578 651 748 939 1018 1604 1699 1998 Size: 134 73 97 191 79 586 95 299 Cuts at: 1998 2136 2550 2798 2833 444 Size: 138 414 248 35 2731 Fragments arranged by size: 2731 586 414 299 248 191 138 134 97 95 79 73 35 BanII G_rGCy'C Cuts at: 827 1162 1451 1529 1745 1862 2134 3164 827 Size: 335 289 78 216 117 272 1030 2783 Fragments arranged by size: 2783 1030 335 289 272 216 117 78 BbeI G_GCGC'C Cuts at: 943 2554 2802 943 Size: 1611 248 3261 Fragments arranged by size: 3261 1611 248 BbsI GAAGACnn'nnnn_ Cuts at: 254 633 254 Size: 379 4741 BbvCI CC'TCA_GC Cuts at: 299 299 Size: 5120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 141 175 141 Size: 34 5086 BciVI GTATCCnnnnn_n' Cuts at: 3018 3779 5120 3018 Size: 761 1341 3018 Fragments arranged by size: 3018 1341 761 BclI T'GATC_A Cuts at: 2640 2640 Size: 5120 BglI GCCn_nnn'nGGC Cuts at: 135 161 937 1074 1083 1104 1143 1332 135 Size: 26 776 137 9 21 39 189 3923 Fragments arranged by size: 3923 776 189 137 39 26 21 9 BglII A'GATC_T Cuts at: 338 2635 4672 338 Size: 2297 2037 786 Fragments arranged by size: 2297 2037 786 BlpI GC'TnA_GC Cuts at: 2232 2232 Size: 5120 Bme1580I G_kGCm'C Cuts at: 656 1162 1337 1702 2745 2838 3894 656 Size: 506 175 365 1043 93 1056 1882 Fragments arranged by size: 1882 1056 1043 506 365 175 93 BmgBI CAC'GTC Cuts at: 1821 1887 1821 Size: 66 5054 BmrI ACTGGGnnnn_n' Cuts at: 379 1159 2332 2413 2748 4892 379 Size: 780 1173 81 335 2144 607 Fragments arranged by size: 2144 1173 780 607 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3487 3487 Size: 5120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 822 900 928 1465 822 Size: 78 28 537 4477 Fragments arranged by size: 4477 537 78 28 Bpu10I CC'TnA_GC Cuts at: 299 1663 299 Size: 1364 3756 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 3175 3667 3965 2296 Size: 879 492 298 3451 Fragments arranged by size: 3451 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 403 1589 4824 403 Size: 1186 3235 699 Fragments arranged by size: 3235 1186 699 BsaAI yAC'GTr Cuts at: 1794 2074 2473 1794 Size: 280 399 4441 Fragments arranged by size: 4441 399 280 BsaBI GATnn'nnATC Cuts at: 6 2634 4671 6 Size: 2628 2037 455 Fragments arranged by size: 2628 2037 455 BsaHI Gr'CG_yC Cuts at: 489 940 1066 2551 2799 3501 4997 489 Size: 451 126 1485 248 702 1496 612 Fragments arranged by size: 1496 1485 702 612 451 248 126 BsaWI w'CCGG_w Cuts at: 391 751 2309 2830 3508 3782 3929 4340 391 Size: 360 1558 521 678 274 147 411 1171 Fragments arranged by size: 1558 1171 678 521 411 360 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 148 187 1594 1975 148 Size: 39 1407 381 3293 Fragments arranged by size: 3293 1407 381 39 BseYI C'CCAG_C Cuts at: 304 888 900 1046 1112 2545 3303 3880 4376 Size: 584 12 146 66 1433 758 577 496 Cuts at: 4376 304 Size: 1048 Fragments arranged by size: 1433 1048 758 584 577 496 146 66 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1874 1972 2068 1874 Size: 98 96 4926 Fragments arranged by size: 4926 98 96 BsiEI CG_ry'CG Cuts at: 104 139 168 187 245 1099 1392 1831 2662 Size: 35 29 19 58 854 293 439 831 Cuts at: 2662 2708 3916 104 Size: 46 1208 1308 Fragments arranged by size: 1308 1208 854 831 439 293 58 46 35 29 19 BsiHKAI G_wGCw'C Cuts at: 401 914 1793 2134 2912 3102 3894 401 Size: 513 879 341 778 190 792 1627 Fragments arranged by size: 1627 879 792 778 513 341 190 Bsp1286I G_dGCh'C Cuts at: 401 656 827 914 1162 1337 1451 1529 1702 Size: 255 171 87 248 175 114 78 173 Cuts at: 1702 1745 1793 1862 2134 2745 2838 2912 3102 Size: 43 48 69 272 611 93 74 190 Cuts at: 3102 3164 3894 401 Size: 62 730 1627 Fragments arranged by size: 1627 730 611 272 255 248 190 175 173 171 114 93 87 78 74 69 62 48 43 BspEI T'CCGG_A Cuts at: 4340 4340 Size: 5120 BspHI T'CATG_A Cuts at: 4296 4296 Size: 5120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 302 488 857 2170 2384 2686 3067 302 Size: 186 369 1313 214 302 381 2355 Fragments arranged by size: 2355 1313 381 369 302 214 186 BsrBI CCG'CTC Cuts at: 50 1143 1188 3412 3466 4950 5115 50 Size: 1093 45 2224 54 1484 165 55 Fragments arranged by size: 2224 1484 1093 165 55 54 45 BsrDI GCAATG_nn' Cuts at: 1674 3032 1674 Size: 1358 3762 BsrFI r'CCGG_y Cuts at: 391 751 1082 1094 3118 3299 3508 391 Size: 360 331 12 2024 181 209 2003 Fragments arranged by size: 2024 2003 360 331 209 181 12 BssHII G'CGCG_C Cuts at: 125 125 Size: 5120 BssSI C'ACGA_G Cuts at: 3391 3749 3391 Size: 358 4762 BstAPI GCAn_nnn'nTGC Cuts at: 2497 2497 Size: 5120 BstBI TT'CG_AA Cuts at: 3481 3481 Size: 5120 BstEII G'GTnAC_C Cuts at: 1411 1411 Size: 5120 BstXI CCAn_nnnn'nTGG Cuts at: 636 636 Size: 5120 BstYI r'GATC_y Cuts at: 1 338 380 1634 2145 2612 2635 2970 3216 Size: 337 42 1254 511 467 23 335 246 Cuts at: 3216 4228 4314 4326 4672 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1254 1012 511 467 449 346 337 335 246 86 42 23 12 BtgI C'CryG_G Cuts at: 107 723 996 1069 1845 107 Size: 616 273 73 776 3382 Fragments arranged by size: 3382 776 616 273 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3070 3251 3414 3070 Size: 181 163 4776 Fragments arranged by size: 4776 181 163 BtsI GCAGTG_nn' Cuts at: 884 2002 2363 884 Size: 1118 361 3641 Fragments arranged by size: 3641 1118 361 DraI TTT'AAA Cuts at: 2119 2119 Size: 5120 DraIII CAC_nnn'GTG Cuts at: 1591 1591 Size: 5120 DrdI GACnn_nn'nnGTC Cuts at: 256 2826 3684 256 Size: 2570 858 1692 Fragments arranged by size: 2570 1692 858 EaeI y'GGCC_r Cuts at: 136 184 242 1075 1096 1238 1511 1828 2705 Size: 48 58 833 21 142 273 317 877 Cuts at: 2705 2879 3270 3297 4642 136 Size: 174 391 27 1345 614 Fragments arranged by size: 1345 877 833 614 391 317 273 174 142 58 48 27 21 EagI C'GGCC_G Cuts at: 136 184 242 1096 1828 2705 136 Size: 48 58 854 732 877 2551 Fragments arranged by size: 2551 877 854 732 58 48 EarI CTCTTCn'nnn_ Cuts at: 401 871 3143 3353 401 Size: 470 2272 210 2168 Fragments arranged by size: 2272 2168 470 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 321 1882 3328 3638 3784 4930 321 Size: 1561 1446 310 146 1146 511 Fragments arranged by size: 1561 1446 1146 511 310 146 EcoICRI GAG'CTC Cuts at: 2132 2132 Size: 5120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 822 900 928 1465 2945 3377 4124 4392 822 Size: 78 28 537 1480 432 747 268 1550 Fragments arranged by size: 1550 1480 747 537 432 268 78 28 EcoO109I rG'GnC_Cy Cuts at: 605 1159 1655 1804 2259 605 Size: 554 496 149 455 3466 Fragments arranged by size: 3466 554 496 455 149 EcoRI G'AATT_C Cuts at: 2124 2124 Size: 5120 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3139 3139 Size: 5120 FseI GG_CCGG'CC Cuts at: 1086 1086 Size: 5120 FspI TGC'GCA Cuts at: 601 2901 601 Size: 2300 2820 HaeII r_GCGC'y Cuts at: 372 501 943 1383 1572 1731 2097 2554 2802 Size: 129 442 440 189 159 366 457 248 Cuts at: 2802 3824 4803 372 Size: 1022 979 689 Fragments arranged by size: 1022 979 689 457 442 440 366 248 189 159 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2962 2992 2994 3024 2962 Size: 30 2 30 5058 Fragments arranged by size: 5058 30 30 2 HincII GTy'rAC Cuts at: 966 1923 2159 966 Size: 957 236 3927 Fragments arranged by size: 3927 957 236 KasI G'GCGC_C Cuts at: 939 2550 2798 939 Size: 1611 248 3261 Fragments arranged by size: 3261 1611 248 KpnI G_GTAC'C Cuts at: 582 1022 1608 2140 582 Size: 440 586 532 3562 Fragments arranged by size: 3562 586 532 440 MluI A'CGCG_T Cuts at: 4553 4553 Size: 5120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 472 3791 3975 472 Size: 3319 184 1617 Fragments arranged by size: 3319 1617 184 MscI TGG'CCA Cuts at: 1240 2881 1240 Size: 1641 3479 MslI CAynn'nnrTG Cuts at: 394 1850 3109 3236 394 Size: 1456 1259 127 2278 Fragments arranged by size: 2278 1456 1259 127 MspA1I CmG'CkG Cuts at: 201 437 1277 1467 2231 2545 2905 3918 4163 Size: 236 840 190 764 314 360 1013 245 Cuts at: 4163 201 Size: 1158 Fragments arranged by size: 1158 1013 840 764 360 314 245 236 190 NaeI GCC'GGC Cuts at: 1084 3301 1084 Size: 2217 2903 NarI GG'CG_CC Cuts at: 940 2551 2799 940 Size: 1611 248 3261 Fragments arranged by size: 3261 1611 248 NcoI C'CATG_G Cuts at: 107 107 Size: 5120 NdeI CA'TA_TG Cuts at: 314 314 Size: 5120 NgoMIV G'CCGG_C Cuts at: 1082 3299 1082 Size: 2217 2903 NotI GC'GGCC_GC Cuts at: 184 184 Size: 5120 NspI r_CATG'y Cuts at: 2173 2673 3580 2173 Size: 500 907 3713 Fragments arranged by size: 3713 907 500 PasI CC'CwG_GG Cuts at: 1600 1600 Size: 5120 PciI A'CATG_T Cuts at: 3576 3576 Size: 5120 PflMI CCAn_nnn'nTGG Cuts at: 342 419 588 900 1520 1709 342 Size: 77 169 312 620 189 3753 Fragments arranged by size: 3753 620 312 189 169 77 PfoI T'CCnGG_A Cuts at: 644 1307 644 Size: 663 4457 PmeI GTTT'AAAC Cuts at: 2119 2119 Size: 5120 PmlI CAC'GTG Cuts at: 1794 2074 1794 Size: 280 4840 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 4259 Size: 32 5088 PspOMI G'GGCC_C Cuts at: 1158 1158 Size: 5120 PstI C_TGCA'G Cuts at: 2105 2167 2852 2105 Size: 62 685 4373 Fragments arranged by size: 4373 685 62 PvuI CG_AT'CG Cuts at: 104 104 Size: 5120 PvuII CAG'CTG Cuts at: 2545 2905 2545 Size: 360 4760 RsrII CG'GwC_CG Cuts at: 3315 3315 Size: 5120 SacI G_AGCT'C Cuts at: 2134 2134 Size: 5120 SalI G'TCGA_C Cuts at: 2157 2157 Size: 5120 SapI GCTCTTCn'nnn_ Cuts at: 3143 3353 3143 Size: 210 4910 SbfI CC_TGCA'GG Cuts at: 2167 2167 Size: 5120 ScaI AGT'ACT Cuts at: 4637 4637 Size: 5120 SfcI C'TryA_G Cuts at: 33 2101 2163 2848 3841 4032 33 Size: 2068 62 685 993 191 1121 Fragments arranged by size: 2068 1121 993 685 191 62 SfiI GGCCn_nnn'nGGCC Cuts at: 1083 1104 1083 Size: 21 5099 SfoI GGC'GCC Cuts at: 941 2552 2800 941 Size: 1611 248 3261 Fragments arranged by size: 3261 1611 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5120 SgrAI Cr'CCGG_yG Cuts at: 391 751 391 Size: 360 4760 SmaI CCC'GGG Cuts at: 1863 2142 1863 Size: 279 4841 SmlI C'TyrA_G Cuts at: 2275 3190 3682 3944 2275 Size: 915 492 262 3451 Fragments arranged by size: 3451 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5120 SphI G_CATG'C Cuts at: 2173 2673 2173 Size: 500 4620 StyI C'CwwG_G Cuts at: 107 231 413 792 2254 2608 107 Size: 124 182 379 1462 354 2619 Fragments arranged by size: 2619 1462 379 354 182 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 820 2703 2836 3295 3504 820 Size: 1883 133 459 209 2436 Fragments arranged by size: 2436 1883 459 209 133 TatI w'GTAC_w Cuts at: 4635 4635 Size: 5120 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4637 4669 4637 Size: 32 5088 Tth111I GACn'n_nGTC Cuts at: 2917 2917 Size: 5120 XbaI T'CTAG_A Cuts at: 2151 2151 Size: 5120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 933 1479 1808 2615 933 Size: 546 329 807 3438 Fragments arranged by size: 3438 807 546 329 XmaI C'CCGG_G Cuts at: 1861 2140 1861 Size: 279 4841 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AloI AscI BaeI BfrBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI NheI NruI NsiI PacI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI