Restriction map for pF1KB6215
 (Circular) (Six-base) MAPSORT of: pF1KB6215.seq  Check: 1964  from: 1  to: 5120



pF1KB6215  5120 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AccI GT'mk_AC

Cuts at:   2158    2158 
   Size:       5120
 
Acc65I G'GTAC_C

Cuts at:    578    1018    1604    2136     578 
   Size:        440     586     532    3562

  Fragments arranged by size: 

                3562     586     532     440
 
AclI AA'CG_TT

Cuts at:   4960    4960 
   Size:       5120
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2945    3377    4124    4392    2945 
   Size:        432     747     268    3673

  Fragments arranged by size: 

                3673     747     432     268
 
AfeI AGC'GCT

Cuts at:    370     499    2095     370 
   Size:        129    1596    3395

  Fragments arranged by size: 

                3395    1596     129
 
AflIII A'CryG_T

Cuts at:   2073    2472    3576    4553    2073 
   Size:        399    1104     977    2640

  Fragments arranged by size: 

                2640    1104     977     399
 
AgeI A'CCGG_T

Cuts at:    391     751    3508     391 
   Size:        360    2757    2003

  Fragments arranged by size: 

                2757    2003     360
 
AleI CACnn'nnGTG

Cuts at:    394    1850     394 
   Size:       1456    3664
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    865     865 
   Size:       5120
 
AlwNI CAG_nnn'CTG

Cuts at:   1467    1565    1625    2425    3992    1467 
   Size:         98      60     800    1567    2595

  Fragments arranged by size: 

                2595    1567     800      98      60
 
ApaI G_GGCC'C

Cuts at:   1162    1162 
   Size:       5120
 
ApaLI G'TGCA_C

Cuts at:   3890    3890 
   Size:       5120
 
ApoI r'AATT_y

Cuts at:   1714    2124    4473    4729    1714 
   Size:        410    2349     256    2105

  Fragments arranged by size: 

                2349    2105     410     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5120
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5120
 
AvaI C'yCGr_G

Cuts at:    120     982    1175    1861    2140     120 
   Size:        862     193     686     279    3100

  Fragments arranged by size: 

                3100     862     686     279     193
 
AvrII C'CTAG_G

Cuts at:    792     792 
   Size:       5120
 
BamHI G'GATC_C

Cuts at:    380    1634    2145     380 
   Size:       1254     511    3355

  Fragments arranged by size: 

                3355    1254     511
 
BanI G'GyrC_C

Cuts at:    444     578     651     748     939    1018    1604    1699    1998 
   Size:        134      73      97     191      79     586      95     299

Cuts at:   1998    2136    2550    2798    2833     444 
   Size:        138     414     248      35    2731

  Fragments arranged by size: 

                2731     586     414     299     248     191     138     134
                  97      95      79      73      35
 
BanII G_rGCy'C

Cuts at:    827    1162    1451    1529    1745    1862    2134    3164     827 
   Size:        335     289      78     216     117     272    1030    2783

  Fragments arranged by size: 

                2783    1030     335     289     272     216     117      78
            
 
BbeI G_GCGC'C

Cuts at:    943    2554    2802     943 
   Size:       1611     248    3261

  Fragments arranged by size: 

                3261    1611     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    254     633     254 
   Size:        379    4741
 
BbvCI CC'TCA_GC

Cuts at:    299     299 
   Size:       5120
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    141     175     141 
   Size:         34    5086
 
BciVI GTATCCnnnnn_n'

Cuts at:   3018    3779    5120    3018 
   Size:        761    1341    3018

  Fragments arranged by size: 

                3018    1341     761
 
BclI T'GATC_A

Cuts at:   2640    2640 
   Size:       5120
 
BglI GCCn_nnn'nGGC

Cuts at:    135     161     937    1074    1083    1104    1143    1332     135 
   Size:         26     776     137       9      21      39     189    3923

  Fragments arranged by size: 

                3923     776     189     137      39      26      21       9
            
 
BglII A'GATC_T

Cuts at:    338    2635    4672     338 
   Size:       2297    2037     786

  Fragments arranged by size: 

                2297    2037     786
 
BlpI GC'TnA_GC

Cuts at:   2232    2232 
   Size:       5120
 
Bme1580I G_kGCm'C

Cuts at:    656    1162    1337    1702    2745    2838    3894     656 
   Size:        506     175     365    1043      93    1056    1882

  Fragments arranged by size: 

                1882    1056    1043     506     365     175      93
 
BmgBI CAC'GTC

Cuts at:   1821    1887    1821 
   Size:         66    5054
 
BmrI ACTGGGnnnn_n'

Cuts at:    379    1159    2332    2413    2748    4892     379 
   Size:        780    1173      81     335    2144     607

  Fragments arranged by size: 

                2144    1173     780     607     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3487    3487 
   Size:       5120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    822     900     928    1465     822 
   Size:         78      28     537    4477

  Fragments arranged by size: 

                4477     537      78      28
 
Bpu10I CC'TnA_GC

Cuts at:    299    1663     299 
   Size:       1364    3756
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2296    3175    3667    3965    2296 
   Size:        879     492     298    3451

  Fragments arranged by size: 

                3451     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    403    1589    4824     403 
   Size:       1186    3235     699

  Fragments arranged by size: 

                3235    1186     699
 
BsaAI yAC'GTr

Cuts at:   1794    2074    2473    1794 
   Size:        280     399    4441

  Fragments arranged by size: 

                4441     399     280
 
BsaBI GATnn'nnATC

Cuts at:      6    2634    4671       6 
   Size:       2628    2037     455

  Fragments arranged by size: 

                2628    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    489     940    1066    2551    2799    3501    4997     489 
   Size:        451     126    1485     248     702    1496     612

  Fragments arranged by size: 

                1496    1485     702     612     451     248     126
 
BsaWI w'CCGG_w

Cuts at:    391     751    2309    2830    3508    3782    3929    4340     391 
   Size:        360    1558     521     678     274     147     411    1171

  Fragments arranged by size: 

                1558    1171     678     521     411     360     274     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    148     187    1594    1975     148 
   Size:         39    1407     381    3293

  Fragments arranged by size: 

                3293    1407     381      39
 
BseYI C'CCAG_C

Cuts at:    304     888     900    1046    1112    2545    3303    3880    4376 
   Size:        584      12     146      66    1433     758     577     496

Cuts at:   4376     304 
   Size:       1048

  Fragments arranged by size: 

                1433    1048     758     584     577     496     146      66
                  12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1874    1972    2068    1874 
   Size:         98      96    4926

  Fragments arranged by size: 

                4926      98      96
 
BsiEI CG_ry'CG

Cuts at:    104     139     168     187     245    1099    1392    1831    2662 
   Size:         35      29      19      58     854     293     439     831

Cuts at:   2662    2708    3916     104 
   Size:         46    1208    1308

  Fragments arranged by size: 

                1308    1208     854     831     439     293      58      46
                  35      29      19
 
BsiHKAI G_wGCw'C

Cuts at:    401     914    1793    2134    2912    3102    3894     401 
   Size:        513     879     341     778     190     792    1627

  Fragments arranged by size: 

                1627     879     792     778     513     341     190
 
Bsp1286I G_dGCh'C

Cuts at:    401     656     827     914    1162    1337    1451    1529    1702 
   Size:        255     171      87     248     175     114      78     173

Cuts at:   1702    1745    1793    1862    2134    2745    2838    2912    3102 
   Size:         43      48      69     272     611      93      74     190

Cuts at:   3102    3164    3894     401 
   Size:         62     730    1627

  Fragments arranged by size: 

                1627     730     611     272     255     248     190     175
                 173     171     114      93      87      78      74      69
                  62      48      43
 
BspEI T'CCGG_A

Cuts at:   4340    4340 
   Size:       5120
 
BspHI T'CATG_A

Cuts at:   4296    4296 
   Size:       5120
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    302     488     857    2170    2384    2686    3067     302 
   Size:        186     369    1313     214     302     381    2355

  Fragments arranged by size: 

                2355    1313     381     369     302     214     186
 
BsrBI CCG'CTC

Cuts at:     50    1143    1188    3412    3466    4950    5115      50 
   Size:       1093      45    2224      54    1484     165      55

  Fragments arranged by size: 

                2224    1484    1093     165      55      54      45
 
BsrDI GCAATG_nn'

Cuts at:   1674    3032    1674 
   Size:       1358    3762
 
BsrFI r'CCGG_y

Cuts at:    391     751    1082    1094    3118    3299    3508     391 
   Size:        360     331      12    2024     181     209    2003

  Fragments arranged by size: 

                2024    2003     360     331     209     181      12
 
BssHII G'CGCG_C

Cuts at:    125     125 
   Size:       5120
 
BssSI C'ACGA_G

Cuts at:   3391    3749    3391 
   Size:        358    4762
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2497    2497 
   Size:       5120
 
BstBI TT'CG_AA

Cuts at:   3481    3481 
   Size:       5120
 
BstEII G'GTnAC_C

Cuts at:   1411    1411 
   Size:       5120
 
BstXI CCAn_nnnn'nTGG

Cuts at:    636     636 
   Size:       5120
 
BstYI r'GATC_y

Cuts at:      1     338     380    1634    2145    2612    2635    2970    3216 
   Size:        337      42    1254     511     467      23     335     246

Cuts at:   3216    4228    4314    4326    4672       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1254    1012     511     467     449     346     337     335
                 246      86      42      23      12
 
BtgI C'CryG_G

Cuts at:    107     723     996    1069    1845     107 
   Size:        616     273      73     776    3382

  Fragments arranged by size: 

                3382     776     616     273      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3070    3251    3414    3070 
   Size:        181     163    4776

  Fragments arranged by size: 

                4776     181     163
 
BtsI GCAGTG_nn'

Cuts at:    884    2002    2363     884 
   Size:       1118     361    3641

  Fragments arranged by size: 

                3641    1118     361
 
DraI TTT'AAA

Cuts at:   2119    2119 
   Size:       5120
 
DraIII CAC_nnn'GTG

Cuts at:   1591    1591 
   Size:       5120
 
DrdI GACnn_nn'nnGTC

Cuts at:    256    2826    3684     256 
   Size:       2570     858    1692

  Fragments arranged by size: 

                2570    1692     858
 
EaeI y'GGCC_r

Cuts at:    136     184     242    1075    1096    1238    1511    1828    2705 
   Size:         48      58     833      21     142     273     317     877

Cuts at:   2705    2879    3270    3297    4642     136 
   Size:        174     391      27    1345     614

  Fragments arranged by size: 

                1345     877     833     614     391     317     273     174
                 142      58      48      27      21
 
EagI C'GGCC_G

Cuts at:    136     184     242    1096    1828    2705     136 
   Size:         48      58     854     732     877    2551

  Fragments arranged by size: 

                2551     877     854     732      58      48
 
EarI CTCTTCn'nnn_

Cuts at:    401     871    3143    3353     401 
   Size:        470    2272     210    2168

  Fragments arranged by size: 

                2272    2168     470     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    321    1882    3328    3638    3784    4930     321 
   Size:       1561    1446     310     146    1146     511

  Fragments arranged by size: 

                1561    1446    1146     511     310     146
 
EcoICRI GAG'CTC

Cuts at:   2132    2132 
   Size:       5120
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    822     900     928    1465    2945    3377    4124    4392     822 
   Size:         78      28     537    1480     432     747     268    1550

  Fragments arranged by size: 

                1550    1480     747     537     432     268      78      28
            
 
EcoO109I rG'GnC_Cy

Cuts at:    605    1159    1655    1804    2259     605 
   Size:        554     496     149     455    3466

  Fragments arranged by size: 

                3466     554     496     455     149
 
EcoRI G'AATT_C

Cuts at:   2124    2124 
   Size:       5120
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3139    3139 
   Size:       5120
 
FseI GG_CCGG'CC

Cuts at:   1086    1086 
   Size:       5120
 
FspI TGC'GCA

Cuts at:    601    2901     601 
   Size:       2300    2820
 
HaeII r_GCGC'y

Cuts at:    372     501     943    1383    1572    1731    2097    2554    2802 
   Size:        129     442     440     189     159     366     457     248

Cuts at:   2802    3824    4803     372 
   Size:       1022     979     689

  Fragments arranged by size: 

                1022     979     689     457     442     440     366     248
                 189     159     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2962    2992    2994    3024    2962 
   Size:         30       2      30    5058

  Fragments arranged by size: 

                5058      30      30       2
 
HincII GTy'rAC

Cuts at:    966    1923    2159     966 
   Size:        957     236    3927

  Fragments arranged by size: 

                3927     957     236
 
KasI G'GCGC_C

Cuts at:    939    2550    2798     939 
   Size:       1611     248    3261

  Fragments arranged by size: 

                3261    1611     248
 
KpnI G_GTAC'C

Cuts at:    582    1022    1608    2140     582 
   Size:        440     586     532    3562

  Fragments arranged by size: 

                3562     586     532     440
 
MluI A'CGCG_T

Cuts at:   4553    4553 
   Size:       5120
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    472    3791    3975     472 
   Size:       3319     184    1617

  Fragments arranged by size: 

                3319    1617     184
 
MscI TGG'CCA

Cuts at:   1240    2881    1240 
   Size:       1641    3479
 
MslI CAynn'nnrTG

Cuts at:    394    1850    3109    3236     394 
   Size:       1456    1259     127    2278

  Fragments arranged by size: 

                2278    1456    1259     127
 
MspA1I CmG'CkG

Cuts at:    201     437    1277    1467    2231    2545    2905    3918    4163 
   Size:        236     840     190     764     314     360    1013     245

Cuts at:   4163     201 
   Size:       1158

  Fragments arranged by size: 

                1158    1013     840     764     360     314     245     236
                 190
 
NaeI GCC'GGC

Cuts at:   1084    3301    1084 
   Size:       2217    2903
 
NarI GG'CG_CC

Cuts at:    940    2551    2799     940 
   Size:       1611     248    3261

  Fragments arranged by size: 

                3261    1611     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       5120
 
NdeI CA'TA_TG

Cuts at:    314     314 
   Size:       5120
 
NgoMIV G'CCGG_C

Cuts at:   1082    3299    1082 
   Size:       2217    2903
 
NotI GC'GGCC_GC

Cuts at:    184     184 
   Size:       5120
 
NspI r_CATG'y

Cuts at:   2173    2673    3580    2173 
   Size:        500     907    3713

  Fragments arranged by size: 

                3713     907     500
 
PasI CC'CwG_GG

Cuts at:   1600    1600 
   Size:       5120
 
PciI A'CATG_T

Cuts at:   3576    3576 
   Size:       5120
 
PflMI CCAn_nnn'nTGG

Cuts at:    342     419     588     900    1520    1709     342 
   Size:         77     169     312     620     189    3753

  Fragments arranged by size: 

                3753     620     312     189     169      77
 
PfoI T'CCnGG_A

Cuts at:    644    1307     644 
   Size:        663    4457
 
PmeI GTTT'AAAC

Cuts at:   2119    2119 
   Size:       5120
 
PmlI CAC'GTG

Cuts at:   1794    2074    1794 
   Size:        280    4840
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4259    4291    4259 
   Size:         32    5088
 
PspOMI G'GGCC_C

Cuts at:   1158    1158 
   Size:       5120
 
PstI C_TGCA'G

Cuts at:   2105    2167    2852    2105 
   Size:         62     685    4373

  Fragments arranged by size: 

                4373     685      62
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5120
 
PvuII CAG'CTG

Cuts at:   2545    2905    2545 
   Size:        360    4760
 
RsrII CG'GwC_CG

Cuts at:   3315    3315 
   Size:       5120
 
SacI G_AGCT'C

Cuts at:   2134    2134 
   Size:       5120
 
SalI G'TCGA_C

Cuts at:   2157    2157 
   Size:       5120
 
SapI GCTCTTCn'nnn_

Cuts at:   3143    3353    3143 
   Size:        210    4910
 
SbfI CC_TGCA'GG

Cuts at:   2167    2167 
   Size:       5120
 
ScaI AGT'ACT

Cuts at:   4637    4637 
   Size:       5120
 
SfcI C'TryA_G

Cuts at:     33    2101    2163    2848    3841    4032      33 
   Size:       2068      62     685     993     191    1121

  Fragments arranged by size: 

                2068    1121     993     685     191      62
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1083    1104    1083 
   Size:         21    5099
 
SfoI GGC'GCC

Cuts at:    941    2552    2800     941 
   Size:       1611     248    3261

  Fragments arranged by size: 

                3261    1611     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5120
 
SgrAI Cr'CCGG_yG

Cuts at:    391     751     391 
   Size:        360    4760
 
SmaI CCC'GGG

Cuts at:   1863    2142    1863 
   Size:        279    4841
 
SmlI C'TyrA_G

Cuts at:   2275    3190    3682    3944    2275 
   Size:        915     492     262    3451

  Fragments arranged by size: 

                3451     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5120
 
SphI G_CATG'C

Cuts at:   2173    2673    2173 
   Size:        500    4620
 
StyI C'CwwG_G

Cuts at:    107     231     413     792    2254    2608     107 
   Size:        124     182     379    1462     354    2619

  Fragments arranged by size: 

                2619    1462     379     354     182     124
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    820    2703    2836    3295    3504     820 
   Size:       1883     133     459     209    2436

  Fragments arranged by size: 

                2436    1883     459     209     133
 
TatI w'GTAC_w

Cuts at:   4635    4635 
   Size:       5120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4637    4669    4637 
   Size:         32    5088
 
Tth111I GACn'n_nGTC

Cuts at:   2917    2917 
   Size:       5120
 
XbaI T'CTAG_A

Cuts at:   2151    2151 
   Size:       5120
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    933    1479    1808    2615     933 
   Size:        546     329     807    3438

  Fragments arranged by size: 

                3438     807     546     329
 
XmaI C'CCGG_G

Cuts at:   1861    2140    1861 
   Size:        279    4841
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AloI     AscI     BaeI    BfrBI
     BmtI    BsaXI    BsiWI     BsmI    BsmBI    BsrGI  BstZ17I   Bsu36I
     ClaI    CspCI    EcoNI    EcoRV    FspAI  HindIII     HpaI     MfeI
     NheI     NruI     NsiI     PacI    PpuMI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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