Restriction map for
pF1KB6219
(Circular) (Six-base) MAPSORT of: pF1KB6219.seq Check: 8469 from: 1 to: 5120
pF1KB6219 5120 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 717 717
Size: 5120
AccI GT'mk_AC
Cuts at: 136 2158 136
Size: 2022 3098
Acc65I G'GTAC_C
Cuts at: 2136 2136
Size: 5120
AclI AA'CG_TT
Cuts at: 4960 4960
Size: 5120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 514 1367 2945 3377 4124 4392 397
Size: 117 853 1578 432 747 268 1125
Fragments arranged by size:
1578 1125 853 747 432 268 117
AflIII A'CryG_T
Cuts at: 2472 3576 4553 2472
Size: 1104 977 3039
Fragments arranged by size:
3039 1104 977
AgeI A'CCGG_T
Cuts at: 3508 3508
Size: 5120
AleI CACnn'nnGTG
Cuts at: 512 1072 512
Size: 560 4560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 841 873 841
Size: 32 5088
AlwNI CAG_nnn'CTG
Cuts at: 558 762 2425 3992 558
Size: 204 1663 1567 1686
Fragments arranged by size:
1686 1663 1567 204
ApaI G_GGCC'C
Cuts at: 1093 2029 2080 1093
Size: 936 51 4133
Fragments arranged by size:
4133 936 51
ApaLI G'TGCA_C
Cuts at: 1483 3890 1483
Size: 2407 2713
ApoI r'AATT_y
Cuts at: 2124 4473 4729 2124
Size: 2349 256 2515
Fragments arranged by size:
2515 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 164 2071 2140 164
Size: 1907 69 3144
Fragments arranged by size:
3144 1907 69
BamHI G'GATC_C
Cuts at: 1268 2145 1268
Size: 877 4243
BanI G'GyrC_C
Cuts at: 1207 1421 1471 2136 2550 2798 2833 1207
Size: 214 50 665 414 248 35 3494
Fragments arranged by size:
3494 665 414 248 214 50 35
BanII G_rGCy'C
Cuts at: 479 736 749 809 1093 1718 1812 1837 2015
Size: 257 13 60 284 625 94 25 178
Cuts at: 2015 2029 2080 2134 3164 479
Size: 14 51 54 1030 2435
Fragments arranged by size:
2435 1030 625 284 257 178 94 60
54 51 25 14 13
BbeI G_GCGC'C
Cuts at: 1211 2554 2802 1211
Size: 1343 248 3529
Fragments arranged by size:
3529 1343 248
BbvCI CC'TCA_GC
Cuts at: 832 1785 832
Size: 953 4167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 862 896 1168 1202 862
Size: 34 272 34 4780
Fragments arranged by size:
4780 272 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3018 3779 5120 3018
Size: 761 1341 3018
Fragments arranged by size:
3018 1341 761
BclI T'GATC_A
Cuts at: 2640 2640
Size: 5120
BglII A'GATC_T
Cuts at: 2635 4672 2635
Size: 2037 3083
BlpI GC'TnA_GC
Cuts at: 988 1753 2100 2232 988
Size: 765 347 132 3876
Fragments arranged by size:
3876 765 347 132
Bme1580I G_kGCm'C
Cuts at: 1093 1474 1487 2029 2080 2745 2838 3894 1093
Size: 381 13 542 51 665 93 1056 2319
Fragments arranged by size:
2319 1056 665 542 381 93 51 13
BmrI ACTGGGnnnn_n'
Cuts at: 823 1089 2332 2413 2748 4892 823
Size: 266 1243 81 335 2144 1051
Fragments arranged by size:
2144 1243 1051 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1200 3487 1200
Size: 2287 2833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 493 707 823 1186 1688 1746 406
Size: 87 214 116 363 502 58 3780
Fragments arranged by size:
3780 502 363 214 116 87 58
Bpu10I CC'TnA_GC
Cuts at: 832 1297 1544 1785 832
Size: 465 247 241 4167
Fragments arranged by size:
4167 465 247 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1732 2296 3175 3667 3965 634
Size: 1098 564 879 492 298 1789
Fragments arranged by size:
1789 1098 879 564 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 190 1138 1869 4824 190
Size: 948 731 2955 486
Fragments arranged by size:
2955 948 731 486
BsaAI yAC'GTr
Cuts at: 2473 2473
Size: 5120
BsaBI GATnn'nnATC
Cuts at: 6 2634 4671 6
Size: 2628 2037 455
Fragments arranged by size:
2628 2037 455
BsaHI Gr'CG_yC
Cuts at: 245 536 1208 2551 2799 3501 4997 245
Size: 291 672 1343 248 702 1496 368
Fragments arranged by size:
1496 1343 702 672 368 291 248
BsaWI w'CCGG_w
Cuts at: 1147 2309 2830 3508 3782 3929 4340 1147
Size: 1162 521 678 274 147 411 1927
Fragments arranged by size:
1927 1162 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 5120
BseYI C'CCAG_C
Cuts at: 114 548 727 1399 1733 1804 1866 1894 1984
Size: 434 179 672 334 71 62 28 90
Cuts at: 1984 2066 2545 3303 3880 4376 114
Size: 82 479 758 577 496 858
Fragments arranged by size:
858 758 672 577 496 479 434 334
179 90 82 71 62 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1267
Size: 5120
BsiEI CG_ry'CG
Cuts at: 104 2662 2708 3916 104
Size: 2558 46 1208 1308
Fragments arranged by size:
2558 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 479 770 1487 1680 1837 2134 2912 3102
Size: 72 291 717 193 157 297 778 190
Cuts at: 3102 3894 407
Size: 792 1633
Fragments arranged by size:
1633 792 778 717 297 291 193 190
157 72
Bsp1286I G_dGCh'C
Cuts at: 407 479 736 749 770 809 1093 1474 1487
Size: 72 257 13 21 39 284 381 13
Cuts at: 1487 1680 1718 1812 1837 2015 2029 2080 2134
Size: 193 38 94 25 178 14 51 54
Cuts at: 2134 2745 2838 2912 3102 3164 3894 407
Size: 611 93 74 190 62 730 1633
Fragments arranged by size:
1633 730 611 381 284 257 193 190
178 94 93 74 72 62 54 51
39 38 25 21 14 13 13
BspEI T'CCGG_A
Cuts at: 4340 4340
Size: 5120
BspHI T'CATG_A
Cuts at: 4296 4296
Size: 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 717 2170 2384 2686 3067 305
Size: 412 1453 214 302 381 2358
Fragments arranged by size:
2358 1453 412 381 302 214
BsrBI CCG'CTC
Cuts at: 50 591 3412 3466 4950 5115 50
Size: 541 2821 54 1484 165 55
Fragments arranged by size:
2821 1484 541 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3032 3032
Size: 5120
BsrFI r'CCGG_y
Cuts at: 182 3118 3299 3508 182
Size: 2936 181 209 1794
Fragments arranged by size:
2936 1794 209 181
BssHII G'CGCG_C
Cuts at: 126 126
Size: 5120
BssSI C'ACGA_G
Cuts at: 3391 3749 3391
Size: 358 4762
BstAPI GCAn_nnn'nTGC
Cuts at: 2497 2497
Size: 5120
BstBI TT'CG_AA
Cuts at: 3481 3481
Size: 5120
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5120
BstYI r'GATC_y
Cuts at: 1 1268 2145 2612 2635 2970 3216 4228 4314
Size: 1267 877 467 23 335 246 1012 86
Cuts at: 4314 4326 4672 1
Size: 12 346 449
Fragments arranged by size:
1267 1012 877 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 140 240 584 630 1592 1689 1799 107
Size: 33 100 344 46 962 97 110 3428
Fragments arranged by size:
3428 962 344 110 100 97 46 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3070 3251 3414 3070
Size: 181 163 4776
Fragments arranged by size:
4776 181 163
BtsI GCAGTG_nn'
Cuts at: 763 1526 2032 2363 763
Size: 763 506 331 3520
Fragments arranged by size:
3520 763 506 331
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 2826 3684 2826
Size: 858 4262
EaeI y'GGCC_r
Cuts at: 656 1313 1412 2705 2879 3270 3297 4642 656
Size: 657 99 1293 174 391 27 1345 1134
Fragments arranged by size:
1345 1293 1134 657 391 174 99 27
EagI C'GGCC_G
Cuts at: 2705 2705
Size: 5120
EarI CTCTTCn'nnn_
Cuts at: 607 1136 1498 1810 3143 3353 607
Size: 529 362 312 1333 210 2374
Fragments arranged by size:
2374 1333 529 362 312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 915 3328 3638 3784 4930 915
Size: 2413 310 146 1146 1105
Fragments arranged by size:
2413 1146 1105 310 146
EcoICRI GAG'CTC
Cuts at: 477 1835 2132 477
Size: 1358 297 3465
Fragments arranged by size:
3465 1358 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 406 493 514 707 823 1186 1367 1688
Size: 9 87 21 193 116 363 181 321
Cuts at: 1688 1746 2945 3377 4124 4392 397
Size: 58 1199 432 747 268 1125
Fragments arranged by size:
1199 1125 747 432 363 321 268 193
181 116 87 58 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 262 1300 1772 262
Size: 1038 472 3610
Fragments arranged by size:
3610 1038 472
EcoO109I rG'GnC_Cy
Cuts at: 381 579 695 824 1089 1090 1363 1637 1779
Size: 198 116 129 265 1 273 274 142
Cuts at: 1779 1820 1889 1980 2026 2076 2077 2259 381
Size: 41 69 91 46 50 1 182 3242
Fragments arranged by size:
3242 274 273 265 198 182 142 129
116 91 69 50 46 41 1 1
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 544 3139 544
Size: 2595 2525
FspI TGC'GCA
Cuts at: 151 301 607 713 2901 151
Size: 150 306 106 2188 2370
Fragments arranged by size:
2370 2188 306 150 106
HaeII r_GCGC'y
Cuts at: 115 254 1211 2554 2802 3824 4803 115
Size: 139 957 1343 248 1022 979 432
Fragments arranged by size:
1343 1022 979 957 432 248 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 476 508 2962 2992 2994 3024 253
Size: 32 191 32 2454 30 2 30 2349
Fragments arranged by size:
2454 2349 191 32 32 30 30 2
HincII GTy'rAC
Cuts at: 137 2159 137
Size: 2022 3098
KasI G'GCGC_C
Cuts at: 1207 2550 2798 1207
Size: 1343 248 3529
Fragments arranged by size:
3529 1343 248
KpnI G_GTAC'C
Cuts at: 2140 2140
Size: 5120
MluI A'CGCG_T
Cuts at: 4553 4553
Size: 5120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 980 1079 3791 3975 416
Size: 564 99 2712 184 1561
Fragments arranged by size:
2712 1561 564 184 99
MscI TGG'CCA
Cuts at: 1414 2881 1414
Size: 1467 3653
MslI CAynn'nnrTG
Cuts at: 512 1072 1591 3109 3236 512
Size: 560 519 1518 127 2396
Fragments arranged by size:
2396 1518 560 519 127
MspA1I CmG'CkG
Cuts at: 216 242 471 632 727 753 1203 1318 1410
Size: 26 229 161 95 26 450 115 92
Cuts at: 1410 1733 2231 2545 2905 3918 4163 216
Size: 323 498 314 360 1013 245 1173
Fragments arranged by size:
1173 1013 498 450 360 323 314 245
229 161 115 95 92 26 26
NaeI GCC'GGC
Cuts at: 184 3301 184
Size: 3117 2003
NarI GG'CG_CC
Cuts at: 1208 2551 2799 1208
Size: 1343 248 3529
Fragments arranged by size:
3529 1343 248
NcoI C'CATG_G
Cuts at: 107 1592 1689 1799 107
Size: 1485 97 110 3428
Fragments arranged by size:
3428 1485 110 97
NdeI CA'TA_TG
Cuts at: 689 689
Size: 5120
NgoMIV G'CCGG_C
Cuts at: 182 3299 182
Size: 3117 2003
NspI r_CATG'y
Cuts at: 306 2173 2673 3580 306
Size: 1867 500 907 1846
Fragments arranged by size:
1867 1846 907 500
PasI CC'CwG_GG
Cuts at: 1237 1634 1966 2016 1237
Size: 397 332 50 4341
Fragments arranged by size:
4341 397 332 50
PciI A'CATG_T
Cuts at: 3576 3576
Size: 5120
PflMI CCAn_nnn'nTGG
Cuts at: 846 948 846
Size: 102 5018
PfoI T'CCnGG_A
Cuts at: 562 1370 1501 562
Size: 808 131 4181
Fragments arranged by size:
4181 808 131
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 5088
PpuMI rG'GwC_Cy
Cuts at: 1363 1779 1820 1889 1980 1363
Size: 416 41 69 91 4503
Fragments arranged by size:
4503 416 91 69 41
PshAI GACnn'nnGTC
Cuts at: 1349 1349
Size: 5120
PspOMI G'GGCC_C
Cuts at: 1089 2025 2076 1089
Size: 936 51 4133
Fragments arranged by size:
4133 936 51
PstI C_TGCA'G
Cuts at: 125 758 1339 1979 2167 2852 125
Size: 633 581 640 188 685 2393
Fragments arranged by size:
2393 685 640 633 581 188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 216 471 727 2545 2905 216
Size: 255 256 1818 360 2431
Fragments arranged by size:
2431 1818 360 256 255
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 479 1837 2134 479
Size: 1358 297 3465
Fragments arranged by size:
3465 1358 297
SacII CC_GC'GG
Cuts at: 243 633 243
Size: 390 4730
SalI G'TCGA_C
Cuts at: 135 2157 135
Size: 2022 3098
SanDI GG'GwC_CC
Cuts at: 1363 1779 1363
Size: 416 4704
SapI GCTCTTCn'nnn_
Cuts at: 3143 3353 3143
Size: 210 4910
SbfI CC_TGCA'GG
Cuts at: 1979 2167 1979
Size: 188 4932
ScaI AGT'ACT
Cuts at: 4637 4637
Size: 5120
SexAI A'CCwGG_T
Cuts at: 335 335
Size: 5120
SfcI C'TryA_G
Cuts at: 33 121 754 897 1335 1975 2163 2848 3841
Size: 88 633 143 438 640 188 685 993
Cuts at: 3841 4032 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 640 633 438 191 188
143 88
SfoI GGC'GCC
Cuts at: 1209 2552 2800 1209
Size: 1343 248 3529
Fragments arranged by size:
3529 1343 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SmaI CCC'GGG
Cuts at: 166 2073 2142 166
Size: 1907 69 3144
Fragments arranged by size:
3144 1907 69
SmlI C'TyrA_G
Cuts at: 649 1711 2275 3190 3682 3944 649
Size: 1062 564 915 492 262 1825
Fragments arranged by size:
1825 1062 915 564 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 306 2173 2673 306
Size: 1867 500 2753
Fragments arranged by size:
2753 1867 500
StuI AGG'CCT
Cuts at: 268 268
Size: 5120
StyI C'CwwG_G
Cuts at: 107 1592 1689 1799 2089 2254 2608 107
Size: 1485 97 110 290 165 354 2619
Fragments arranged by size:
2619 1485 354 290 165 110 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 806 2703 2836 3295 3504 214
Size: 592 1897 133 459 209 1830
Fragments arranged by size:
1897 1830 592 459 209 133
TatI w'GTAC_w
Cuts at: 935 4635 935
Size: 3700 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1276 1308 4637 4669 1276
Size: 32 3329 32 1727
Fragments arranged by size:
3329 1727 32 32
Tth111I GACn'n_nGTC
Cuts at: 558 961 2917 558
Size: 403 1956 2761
Fragments arranged by size:
2761 1956 403
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 570 1407 2615 570
Size: 837 1208 3075
Fragments arranged by size:
3075 1208 837
XmaI C'CCGG_G
Cuts at: 164 2071 2140 164
Size: 1907 69 3144
Fragments arranged by size:
3144 1907 69
XmnI GAAnn'nnTTC
Cuts at: 1913 1913
Size: 5120
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PmlI PsiI PspXI PsrI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI