Restriction map for
pF1KB6223
(Circular) (Six-base) MAPSORT of: pF1KB6223.seq Check: 3177 from: 1 to: 5123
pF1KB6223 5123 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 1092 1092
Size: 5123
AccI GT'mk_AC
Cuts at: 2161 2161
Size: 5123
Acc65I G'GTAC_C
Cuts at: 2139 2139
Size: 5123
AclI AA'CG_TT
Cuts at: 4963 4963
Size: 5123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 866 2126 2948 3380 4127 4395 519
Size: 347 1260 822 432 747 268 1247
Fragments arranged by size:
1260 1247 822 747 432 347 268
AfeI AGC'GCT
Cuts at: 1735 1735
Size: 5123
AflIII A'CryG_T
Cuts at: 297 2475 3579 4556 297
Size: 2178 1104 977 864
Fragments arranged by size:
2178 1104 977 864
AgeI A'CCGG_T
Cuts at: 3511 3511
Size: 5123
AleI CACnn'nnGTG
Cuts at: 416 1517 416
Size: 1101 4022
AlwNI CAG_nnn'CTG
Cuts at: 2428 3995 2428
Size: 1567 3556
ApaLI G'TGCA_C
Cuts at: 3893 3893
Size: 5123
ApoI r'AATT_y
Cuts at: 920 1069 1424 1567 1676 2127 4476 4732 920
Size: 149 355 143 109 451 2349 256 1311
Fragments arranged by size:
2349 1311 451 355 256 149 143 109
AseI AT'TA_AT
Cuts at: 20 20
Size: 5123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
AvaI C'yCGr_G
Cuts at: 2143 2143
Size: 5123
BamHI G'GATC_C
Cuts at: 2148 2148
Size: 5123
BanI G'GyrC_C
Cuts at: 2139 2553 2801 2836 2139
Size: 414 248 35 4426
Fragments arranged by size:
4426 414 248 35
BanII G_rGCy'C
Cuts at: 154 242 584 1906 2137 3167 154
Size: 88 342 1322 231 1030 2110
Fragments arranged by size:
2110 1322 1030 342 231 88
BbeI G_GCGC'C
Cuts at: 2557 2805 2557
Size: 248 4875
BbvCI CC'TCA_GC
Cuts at: 172 172
Size: 5123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5089
BciVI GTATCCnnnnn_n'
Cuts at: 3021 3782 5123 3021
Size: 761 1341 3021
Fragments arranged by size:
3021 1341 761
BclI T'GATC_A
Cuts at: 2643 2643
Size: 5123
BglII A'GATC_T
Cuts at: 2638 4675 2638
Size: 2037 3086
BlpI GC'TnA_GC
Cuts at: 467 2235 467
Size: 1768 3355
Bme1580I G_kGCm'C
Cuts at: 2748 2841 3897 2748
Size: 93 1056 3974
Fragments arranged by size:
3974 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 433 701 2335 2416 2751 4895 433
Size: 268 1634 81 335 2144 661
Fragments arranged by size:
2144 1634 661 335 268 81
BmtI G_CTAG'C
Cuts at: 1826 1826
Size: 5123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3490 3490
Size: 5123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1034 1118 1202 1286 1370 1622 1790 1874
Size: 730 84 84 84 84 252 168 84
Cuts at: 1874 1958 2042 304
Size: 84 84 3385
Fragments arranged by size:
3385 730 252 168 84 84 84 84
84 84 84
Bpu10I CC'TnA_GC
Cuts at: 172 185 172
Size: 13 5110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2299 3178 3670 3968 2299
Size: 879 492 298 3454
Fragments arranged by size:
3454 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4827 4827
Size: 5123
BsaAI yAC'GTr
Cuts at: 2476 2476
Size: 5123
BsaBI GATnn'nnATC
Cuts at: 6 2637 4674 6
Size: 2631 2037 455
Fragments arranged by size:
2631 2037 455
BsaHI Gr'CG_yC
Cuts at: 1089 2554 2802 3504 5000 1089
Size: 1465 248 702 1496 1212
Fragments arranged by size:
1496 1465 1212 702 248
BsaWI w'CCGG_w
Cuts at: 1670 1754 2312 2833 3511 3785 3932 4343 1670
Size: 84 558 521 678 274 147 411 2450
Fragments arranged by size:
2450 678 558 521 411 274 147 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 730 1115 730
Size: 385 4738
BseYI C'CCAG_C
Cuts at: 669 2548 3306 3883 4379 669
Size: 1879 758 577 496 1413
Fragments arranged by size:
1879 1413 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1389 1557 1725 1977 1389
Size: 168 168 252 4535
Fragments arranged by size:
4535 252 168 168
BsiEI CG_ry'CG
Cuts at: 104 122 126 2665 2711 3919 104
Size: 18 4 2539 46 1208 1308
Fragments arranged by size:
2539 1308 1208 46 18 4
BsiHKAI G_wGCw'C
Cuts at: 154 242 473 1906 2137 2915 3105 3897 154
Size: 88 231 1433 231 778 190 792 1380
Fragments arranged by size:
1433 1380 792 778 231 231 190 88
BsmI GAATG_Cn'
Cuts at: 1036 1456 1508 1624 2044 1036
Size: 420 52 116 420 4115
Fragments arranged by size:
4115 420 420 116 52
BsmBI CGTCTCn'nnnn_
Cuts at: 1080 1080
Size: 5123
Bsp1286I G_dGCh'C
Cuts at: 154 242 473 584 1906 2137 2748 2841 2915
Size: 88 231 111 1322 231 611 93 74
Cuts at: 2915 3105 3167 3897 154
Size: 190 62 730 1380
Fragments arranged by size:
1380 1322 730 611 231 231 190 111
93 88 74 62
BspEI T'CCGG_A
Cuts at: 4343 4343
Size: 5123
BspHI T'CATG_A
Cuts at: 1110 4299 1110
Size: 3189 1934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2173 2387 2689 3070 2173
Size: 214 302 381 4226
Fragments arranged by size:
4226 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 3415 3469 4953 5118 50
Size: 81 3284 54 1484 165 55
Fragments arranged by size:
3284 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 3035 3035
Size: 5123
BsrFI r'CCGG_y
Cuts at: 619 3121 3302 3511 619
Size: 2502 181 209 2231
Fragments arranged by size:
2502 2231 209 181
BssSI C'ACGA_G
Cuts at: 3394 3752 3394
Size: 358 4765
BstAPI GCAn_nnn'nTGC
Cuts at: 980 2500 980
Size: 1520 3603
BstBI TT'CG_AA
Cuts at: 3484 3484
Size: 5123
BstYI r'GATC_y
Cuts at: 1 2148 2615 2638 2973 3219 4231 4317 4329
Size: 2147 467 23 335 246 1012 86 12
Cuts at: 4329 4675 1
Size: 346 449
Fragments arranged by size:
2147 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 145 321 677 145
Size: 176 356 4591
Fragments arranged by size:
4591 356 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 287 3073 3254 3417 287
Size: 2786 181 163 1993
Fragments arranged by size:
2786 1993 181 163
BtsI GCAGTG_nn'
Cuts at: 1041 1377 1545 1629 1713 2049 2366 1041
Size: 336 168 84 84 336 317 3798
Fragments arranged by size:
3798 336 336 317 168 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 214 249 214
Size: 35 5088
DraI TTT'AAA
Cuts at: 2122 2122
Size: 5123
DrdI GACnn_nn'nnGTC
Cuts at: 2829 3687 2829
Size: 858 4265
EaeI y'GGCC_r
Cuts at: 123 209 2708 2882 3273 3300 4645 123
Size: 86 2499 174 391 27 1345 601
Fragments arranged by size:
2499 1345 601 391 174 86 27
EagI C'GGCC_G
Cuts at: 123 2708 123
Size: 2585 2538
EarI CTCTTCn'nnn_
Cuts at: 224 572 1332 3146 3356 224
Size: 348 760 1814 210 1991
Fragments arranged by size:
1991 1814 760 348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3331 3641 3787 4933 3331
Size: 310 146 1146 3521
Fragments arranged by size:
3521 1146 310 146
EcoICRI GAG'CTC
Cuts at: 152 240 1904 2135 152
Size: 88 1664 231 3140
Fragments arranged by size:
3140 1664 231 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 519 866 1034 1118 1202 1286 1370 1622
Size: 215 347 168 84 84 84 84 252
Cuts at: 1622 1790 1874 1958 2042 2126 2948 3380 4127
Size: 168 84 84 84 84 822 432 747
Cuts at: 4127 4395 304
Size: 268 1032
Fragments arranged by size:
1032 822 747 432 347 268 252 215
168 168 84 84 84 84 84 84
84 84
EcoNI CCTnn'n_nnAGG
Cuts at: 588 588
Size: 5123
EcoO109I rG'GnC_Cy
Cuts at: 2262 2262
Size: 5123
EcoRI G'AATT_C
Cuts at: 1069 1424 1676 2127 1069
Size: 355 252 451 4065
Fragments arranged by size:
4065 451 355 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 519 871 3142 519
Size: 352 2271 2500
Fragments arranged by size:
2500 2271 352
FspI TGC'GCA
Cuts at: 2904 2904
Size: 5123
HaeII r_GCGC'y
Cuts at: 1737 2557 2805 3827 4806 1737
Size: 820 248 1022 979 2054
Fragments arranged by size:
2054 1022 979 820 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2965 2995 2997 3027 2965
Size: 30 2 30 5061
Fragments arranged by size:
5061 30 30 2
HincII GTy'rAC
Cuts at: 2162 2162
Size: 5123
HindIII A'AGCT_T
Cuts at: 797 797
Size: 5123
KasI G'GCGC_C
Cuts at: 2553 2801 2553
Size: 248 4875
KpnI G_GTAC'C
Cuts at: 2143 2143
Size: 5123
MluI A'CGCG_T
Cuts at: 4556 4556
Size: 5123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 714 3794 3978 714
Size: 3080 184 1859
Fragments arranged by size:
3080 1859 184
MscI TGG'CCA
Cuts at: 211 2884 211
Size: 2673 2450
MslI CAynn'nnrTG
Cuts at: 416 517 569 833 1115 1517 3112 3239 416
Size: 101 52 264 282 402 1595 127 2300
Fragments arranged by size:
2300 1595 402 282 264 127 101 52
MspA1I CmG'CkG
Cuts at: 2234 2548 2908 3921 4166 2234
Size: 314 360 1013 245 3191
Fragments arranged by size:
3191 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3304 3304
Size: 5123
NarI GG'CG_CC
Cuts at: 2554 2802 2554
Size: 248 4875
NcoI C'CATG_G
Cuts at: 321 321
Size: 5123
NgoMIV G'CCGG_C
Cuts at: 3302 3302
Size: 5123
NheI G'CTAG_C
Cuts at: 1822 1822
Size: 5123
NspI r_CATG'y
Cuts at: 301 2176 2676 3583 301
Size: 1875 500 907 1841
Fragments arranged by size:
1875 1841 907 500
PasI CC'CwG_GG
Cuts at: 309 309
Size: 5123
PciI A'CATG_T
Cuts at: 297 3579 297
Size: 3282 1841
PflMI CCAn_nnn'nTGG
Cuts at: 327 327
Size: 5123
PmeI GTTT'AAAC
Cuts at: 2122 2122
Size: 5123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 825 857 4262 4294 825
Size: 32 3405 32 1654
Fragments arranged by size:
3405 1654 32 32
PstI C_TGCA'G
Cuts at: 628 2170 2855 628
Size: 1542 685 2896
Fragments arranged by size:
2896 1542 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5123
PvuII CAG'CTG
Cuts at: 2548 2908 2548
Size: 360 4763
RsrII CG'GwC_CG
Cuts at: 3318 3318
Size: 5123
SacI G_AGCT'C
Cuts at: 154 242 1906 2137 154
Size: 88 1664 231 3140
Fragments arranged by size:
3140 1664 231 88
SalI G'TCGA_C
Cuts at: 2160 2160
Size: 5123
SapI GCTCTTCn'nnn_
Cuts at: 572 3146 3356 572
Size: 2574 210 2339
Fragments arranged by size:
2574 2339 210
SbfI CC_TGCA'GG
Cuts at: 2170 2170
Size: 5123
ScaI AGT'ACT
Cuts at: 4640 4640
Size: 5123
SfcI C'TryA_G
Cuts at: 33 624 2166 2851 3844 4035 33
Size: 591 1542 685 993 191 1121
Fragments arranged by size:
1542 1121 993 685 591 191
SfoI GGC'GCC
Cuts at: 2555 2803 2555
Size: 248 4875
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
SmaI CCC'GGG
Cuts at: 2145 2145
Size: 5123
SmlI C'TyrA_G
Cuts at: 2278 3193 3685 3947 2278
Size: 915 492 262 3454
Fragments arranged by size:
3454 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5123
SphI G_CATG'C
Cuts at: 2176 2676 2176
Size: 500 4623
StuI AGG'CCT
Cuts at: 664 1023 1191 1275 1527 1779 1863 2031 2115
Size: 359 168 84 252 252 84 168 84
Cuts at: 2115 664
Size: 3672
Fragments arranged by size:
3672 359 252 252 168 168 84 84
84
StyI C'CwwG_G
Cuts at: 321 2257 2611 321
Size: 1936 354 2833
Fragments arranged by size:
2833 1936 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 415 2706 2839 3298 3507 411
Size: 4 2291 133 459 209 2027
Fragments arranged by size:
2291 2027 459 209 133 4
TatI w'GTAC_w
Cuts at: 266 4638 266
Size: 4372 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4640 4672 4640
Size: 32 5091
Tth111I GACn'n_nGTC
Cuts at: 2920 2920
Size: 5123
XbaI T'CTAG_A
Cuts at: 2154 2154
Size: 5123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2618 2618
Size: 5123
XmaI C'CCGG_G
Cuts at: 2143 2143
Size: 5123
XmnI GAAnn'nnTTC
Cuts at: 646 798 883 1073 1409 646
Size: 152 85 190 336 4360
Fragments arranged by size:
4360 336 190 152 85
ZraI GAC'GTC
Cuts at: 1090 1090
Size: 5123
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BglI BmgBI BsaXI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI