Restriction map for
pF1KB6227
(Circular) (Six-base) MAPSORT of: pF1KB6227.seq Check: 9584 from: 1 to: 5129
pF1KB6227 5129 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 517 517
Size: 5129
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5129
Acc65I G'GTAC_C
Cuts at: 2145 2145
Size: 5129
AclI AA'CG_TT
Cuts at: 521 1769 4969 521
Size: 1248 3200 681
Fragments arranged by size:
3200 1248 681
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1091 1237 1519 2954 3386 4133 4401 791
Size: 300 146 282 1435 432 747 268 1519
Fragments arranged by size:
1519 1435 747 432 300 282 268 146
AflIII A'CryG_T
Cuts at: 2481 3585 4562 2481
Size: 1104 977 3048
Fragments arranged by size:
3048 1104 977
AgeI A'CCGG_T
Cuts at: 3517 3517
Size: 5129
AhdI GACnn_n'nnGTC
Cuts at: 2011 2011
Size: 5129
AlwNI CAG_nnn'CTG
Cuts at: 263 557 2434 4001 263
Size: 294 1877 1567 1391
Fragments arranged by size:
1877 1567 1391 294
ApaLI G'TGCA_C
Cuts at: 199 1254 2098 3899 199
Size: 1055 844 1801 1429
Fragments arranged by size:
1801 1429 1055 844
ApoI r'AATT_y
Cuts at: 220 346 1018 1408 2133 4482 4738 220
Size: 126 672 390 725 2349 256 611
Fragments arranged by size:
2349 725 672 611 390 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 5129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 123 2149 123
Size: 2026 3103
BamHI G'GATC_C
Cuts at: 666 877 2154 666
Size: 211 1277 3641
Fragments arranged by size:
3641 1277 211
BanI G'GyrC_C
Cuts at: 338 2145 2559 2807 2842 338
Size: 1807 414 248 35 2625
Fragments arranged by size:
2625 1807 414 248 35
BanII G_rGCy'C
Cuts at: 2143 3173 2143
Size: 1030 4099
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 1079 1742 1079
Size: 663 4466
BbvCI CC'TCA_GC
Cuts at: 184 605 184
Size: 421 4708
BciVI GTATCCnnnnn_n'
Cuts at: 3027 3788 5129 3027
Size: 761 1341 3027
Fragments arranged by size:
3027 1341 761
BclI T'GATC_A
Cuts at: 2028 2649 2028
Size: 621 4508
BglI GCCn_nnn'nGGC
Cuts at: 1046 1046
Size: 5129
BglII A'GATC_T
Cuts at: 2644 4681 2644
Size: 2037 3092
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 203 1189 1258 2102 2754 2847 3903 203
Size: 986 69 844 652 93 1056 1429
Fragments arranged by size:
1429 1056 986 844 652 93 69
BmgBI CAC'GTC
Cuts at: 153 1248 1878 153
Size: 1095 630 3404
Fragments arranged by size:
3404 1095 630
BmrI ACTGGGnnnn_n'
Cuts at: 855 1920 2085 2341 2422 2757 4901 855
Size: 1065 165 256 81 335 2144 1083
Fragments arranged by size:
2144 1083 1065 335 256 165 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1129 3496 1129
Size: 2367 2762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 1942 1595
Size: 347 4782
Bpu10I CC'TnA_GC
Cuts at: 184 605 184
Size: 421 4708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 3184 3676 3974 2305
Size: 879 492 298 3460
Fragments arranged by size:
3460 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4833 4833
Size: 5129
BsaAI yAC'GTr
Cuts at: 2482 2482
Size: 5129
BsaBI GATnn'nnATC
Cuts at: 6 1450 2643 4680 6
Size: 1444 1193 2037 455
Fragments arranged by size:
2037 1444 1193 455
BsaHI Gr'CG_yC
Cuts at: 142 1682 2560 2808 3510 5006 142
Size: 1540 878 248 702 1496 265
Fragments arranged by size:
1540 1496 878 702 265 248
BsaWI w'CCGG_w
Cuts at: 978 1898 2318 2839 3517 3791 3938 4349 978
Size: 920 420 521 678 274 147 411 1758
Fragments arranged by size:
1758 920 678 521 420 411 274 147
BseYI C'CCAG_C
Cuts at: 550 1836 1848 2554 3312 3889 4385 550
Size: 1286 12 706 758 577 496 1294
Fragments arranged by size:
1294 1286 758 706 577 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 1300 2138 249
Size: 1051 838 3240
Fragments arranged by size:
3240 1051 838
BsiEI CG_ry'CG
Cuts at: 104 2671 2717 3925 104
Size: 2567 46 1208 1308
Fragments arranged by size:
2567 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 1258 1766 2102 2143 2921 3111 3903 203
Size: 1055 508 336 41 778 190 792 1429
Fragments arranged by size:
1429 1055 792 778 508 336 190 41
BsmBI CGTCTCn'nnnn_
Cuts at: 149 149
Size: 5129
Bsp1286I G_dGCh'C
Cuts at: 203 1189 1258 1766 2102 2143 2754 2847 2921
Size: 986 69 508 336 41 611 93 74
Cuts at: 2921 3111 3173 3903 203
Size: 190 62 730 1429
Fragments arranged by size:
1429 986 730 611 508 336 190 93
74 69 62 41
BspEI T'CCGG_A
Cuts at: 978 1898 4349 978
Size: 920 2451 1758
Fragments arranged by size:
2451 1758 920
BspHI T'CATG_A
Cuts at: 4305 4305
Size: 5129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 2179 2393 2695 3076 517
Size: 1662 214 302 381 2570
Fragments arranged by size:
2570 1662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 755 3421 3475 4959 5124 50
Size: 705 2666 54 1484 165 55
Fragments arranged by size:
2666 1484 705 165 55 54
BsrDI GCAATG_nn'
Cuts at: 538 3041 538
Size: 2503 2626
BsrFI r'CCGG_y
Cuts at: 915 3127 3308 3517 915
Size: 2212 181 209 2527
Fragments arranged by size:
2527 2212 209 181
BssHII G'CGCG_C
Cuts at: 229 229
Size: 5129
BssSI C'ACGA_G
Cuts at: 202 2000 3400 3758 202
Size: 1798 1400 358 1573
Fragments arranged by size:
1798 1573 1400 358
BstAPI GCAn_nnn'nTGC
Cuts at: 263 2506 263
Size: 2243 2886
BstBI TT'CG_AA
Cuts at: 3490 3490
Size: 5129
BstXI CCAn_nnnn'nTGG
Cuts at: 1848 1848
Size: 5129
BstYI r'GATC_y
Cuts at: 1 666 684 877 1494 1934 2154 2621 2644
Size: 665 18 193 617 440 220 467 23
Cuts at: 2644 2979 3225 4237 4323 4335 4681 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 665 617 467 449 440 346 335
246 220 193 86 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 1862 1862
Size: 5129
BtgI C'CryG_G
Cuts at: 107 137 1259 107
Size: 30 1122 3977
Fragments arranged by size:
3977 1122 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 210 3079 3260 3423 210
Size: 2869 181 163 1916
Fragments arranged by size:
2869 1916 181 163
BtsI GCAGTG_nn'
Cuts at: 261 466 778 2372 261
Size: 205 312 1594 3018
Fragments arranged by size:
3018 1594 312 205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1944 1979 1944
Size: 35 5094
DraI TTT'AAA
Cuts at: 2128 2128
Size: 5129
DraIII CAC_nnn'GTG
Cuts at: 207 207
Size: 5129
DrdI GACnn_nn'nnGTC
Cuts at: 2835 3693 2835
Size: 858 4271
EaeI y'GGCC_r
Cuts at: 155 917 2714 2888 3279 3306 4651 155
Size: 762 1797 174 391 27 1345 633
Fragments arranged by size:
1797 1345 762 633 391 174 27
EagI C'GGCC_G
Cuts at: 2714 2714
Size: 5129
EarI CTCTTCn'nnn_
Cuts at: 574 3152 3362 574
Size: 2578 210 2341
Fragments arranged by size:
2578 2341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 603 3337 3647 3793 4939 603
Size: 2734 310 146 1146 793
Fragments arranged by size:
2734 1146 793 310 146
EcoICRI GAG'CTC
Cuts at: 2141 2141
Size: 5129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1091 1237 1519 1595 1942 2954 3386 4133
Size: 300 146 282 76 347 1012 432 747
Cuts at: 4133 4401 791
Size: 268 1519
Fragments arranged by size:
1519 1012 747 432 347 300 282 268
146 76
EcoNI CCTnn'n_nnAGG
Cuts at: 1219 1219
Size: 5129
EcoO109I rG'GnC_Cy
Cuts at: 382 1377 1595 2268 382
Size: 995 218 673 3243
Fragments arranged by size:
3243 995 673 218
EcoRI G'AATT_C
Cuts at: 2133 2133
Size: 5129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1051 1293 3148 1051
Size: 242 1855 3032
Fragments arranged by size:
3032 1855 242
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 184 2563 2811 3833 4812 184
Size: 2379 248 1022 979 501
Fragments arranged by size:
2379 1022 979 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1323 1355 1997 2029 2971 3001 3003 3033 1323
Size: 32 642 32 942 30 2 30 3419
Fragments arranged by size:
3419 942 642 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1368 2085 2168 1368
Size: 717 83 4329
Fragments arranged by size:
4329 717 83
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 2149 2149
Size: 5129
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1499 3800 3984 1115
Size: 384 2301 184 2260
Fragments arranged by size:
2301 2260 384 184
MscI TGG'CCA
Cuts at: 157 2890 157
Size: 2733 2396
MslI CAynn'nnrTG
Cuts at: 3118 3245 3118
Size: 127 5002
MspA1I CmG'CkG
Cuts at: 1314 2034 2240 2554 2914 3927 4172 1314
Size: 720 206 314 360 1013 245 2271
Fragments arranged by size:
2271 1013 720 360 314 245 206
NaeI GCC'GGC
Cuts at: 917 3310 917
Size: 2393 2736
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NcoI C'CATG_G
Cuts at: 107 1259 107
Size: 1152 3977
NgoMIV G'CCGG_C
Cuts at: 915 3308 915
Size: 2393 2736
NspI r_CATG'y
Cuts at: 1891 2182 2682 3589 1891
Size: 291 500 907 3431
Fragments arranged by size:
3431 907 500 291
PciI A'CATG_T
Cuts at: 3585 3585
Size: 5129
PflMI CCAn_nnn'nTGG
Cuts at: 926 1335 1723 926
Size: 409 388 4332
Fragments arranged by size:
4332 409 388
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 4268
Size: 32 5097
PpuMI rG'GwC_Cy
Cuts at: 382 1377 1595 382
Size: 995 218 3916
Fragments arranged by size:
3916 995 218
PshAI GACnn'nnGTC
Cuts at: 825 825
Size: 5129
PstI C_TGCA'G
Cuts at: 259 2176 2861 259
Size: 1917 685 2527
Fragments arranged by size:
2527 1917 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 1314 2034 2554 2914 1314
Size: 720 520 360 3529
Fragments arranged by size:
3529 720 520 360
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 2143 2143
Size: 5129
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5129
SanDI GG'GwC_CC
Cuts at: 382 382
Size: 5129
SapI GCTCTTCn'nnn_
Cuts at: 3152 3362 3152
Size: 210 4919
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 961 4646 961
Size: 3685 1444
SfcI C'TryA_G
Cuts at: 33 255 830 2172 2857 3850 4041 33
Size: 222 575 1342 685 993 191 1121
Fragments arranged by size:
1342 1121 993 685 575 222 191
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 125 2151 125
Size: 2026 3103
SmlI C'TyrA_G
Cuts at: 2284 3199 3691 3953 2284
Size: 915 492 262 3460
Fragments arranged by size:
3460 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5129
SphI G_CATG'C
Cuts at: 1891 2182 2682 1891
Size: 291 500 4338
Fragments arranged by size:
4338 500 291
StyI C'CwwG_G
Cuts at: 107 1259 1793 2263 2617 107
Size: 1152 534 470 354 2619
Fragments arranged by size:
2619 1152 534 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 833 1118 1850 2712 2845 3304 3513 833
Size: 285 732 862 133 459 209 2449
Fragments arranged by size:
2449 862 732 459 285 209 133
TatI w'GTAC_w
Cuts at: 959 1346 4644 959
Size: 387 3298 1444
Fragments arranged by size:
3298 1444 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 486 518 750 782 1587 1619 1646 1678 1756
Size: 32 232 32 805 32 27 32 78
Cuts at: 1756 1788 4646 4678 486
Size: 32 2858 32 937
Fragments arranged by size:
2858 937 805 232 78 32 32 32
32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 2160 2160
Size: 5129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2624 2624
Size: 5129
XmaI C'CCGG_G
Cuts at: 123 2149 123
Size: 2026 3103
XmnI GAAnn'nnTTC
Cuts at: 868 868
Size: 5129
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BseRI BsiWI
BsmI BsrGI BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI