Restriction map for
pF1KB6228
(Circular) (Six-base) MAPSORT of: pF1KB6228.seq Check: 3234 from: 1 to: 5333
pF1KB6228 5333 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 1092 1092
Size: 5333
AccI GT'mk_AC
Cuts at: 482 2371 482
Size: 1889 3444
Acc65I G'GTAC_C
Cuts at: 2349 2349
Size: 5333
AclI AA'CG_TT
Cuts at: 5173 5173
Size: 5333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1643 3158 3590 4337 4605 1511
Size: 132 1515 432 747 268 2239
Fragments arranged by size:
2239 1515 747 432 268 132
AflIII A'CryG_T
Cuts at: 2685 3789 4766 2685
Size: 1104 977 3252
Fragments arranged by size:
3252 1104 977
AgeI A'CCGG_T
Cuts at: 3721 3721
Size: 5333
AleI CACnn'nnGTG
Cuts at: 2161 2161
Size: 5333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 404 148
Size: 256 5077
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 639 671 639
Size: 32 5301
AlwNI CAG_nnn'CTG
Cuts at: 690 946 1171 1489 2146 2323 2638 4205 690
Size: 256 225 318 657 177 315 1567 1818
Fragments arranged by size:
1818 1567 657 318 315 256 225 177
ApaI G_GGCC'C
Cuts at: 179 456 1363 179
Size: 277 907 4149
Fragments arranged by size:
4149 907 277
ApaLI G'TGCA_C
Cuts at: 4103 4103
Size: 5333
ApoI r'AATT_y
Cuts at: 2337 4686 4942 2337
Size: 2349 256 2728
Fragments arranged by size:
2728 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
AvaI C'yCGr_G
Cuts at: 449 2353 449
Size: 1904 3429
BamHI G'GATC_C
Cuts at: 2358 2358
Size: 5333
BanI G'GyrC_C
Cuts at: 889 1184 1306 1385 2349 2763 3011 3046 889
Size: 295 122 79 964 414 248 35 3176
Fragments arranged by size:
3176 964 414 295 248 122 79 35
BanII G_rGCy'C
Cuts at: 179 456 497 572 1296 1363 1594 1895 2347
Size: 277 41 75 724 67 231 301 452
Cuts at: 2347 3377 179
Size: 1030 2135
Fragments arranged by size:
2135 1030 724 452 301 277 231 75
67 41
BbeI G_GCGC'C
Cuts at: 1188 2767 3015 1188
Size: 1579 248 3506
Fragments arranged by size:
3506 1579 248
BbsI GAAGACnn'nnnn_
Cuts at: 1427 1427
Size: 5333
BciVI GTATCCnnnnn_n'
Cuts at: 1830 3231 3992 5333 1830
Size: 1401 761 1341 1830
Fragments arranged by size:
1830 1401 1341 761
BclI T'GATC_A
Cuts at: 2853 2853
Size: 5333
BglI GCCn_nnn'nGGC
Cuts at: 2253 2253
Size: 5333
BglII A'GATC_T
Cuts at: 1610 1654 2848 4885 1610
Size: 44 1194 2037 2058
Fragments arranged by size:
2058 2037 1194 44
BlpI GC'TnA_GC
Cuts at: 167 2445 167
Size: 2278 3055
Bme1580I G_kGCm'C
Cuts at: 179 393 456 1363 1390 2958 3051 4107 179
Size: 214 63 907 27 1568 93 1056 1405
Fragments arranged by size:
1568 1405 1056 907 214 93 63 27
BmrI ACTGGGnnnn_n'
Cuts at: 1980 2134 2545 2626 2961 5105 1980
Size: 154 411 81 335 2144 2208
Fragments arranged by size:
2208 2144 411 335 154 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1251 1310 1315 3700 1251
Size: 59 5 2385 2884
Fragments arranged by size:
2884 2385 59 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1018 1383 1713 1798 2198 310
Size: 708 365 330 85 400 3445
Fragments arranged by size:
3445 708 400 365 330 85
Bpu10I CC'TnA_GC
Cuts at: 1046 1046
Size: 5333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 2509 3388 3880 4178 493
Size: 2016 879 492 298 1648
Fragments arranged by size:
2016 1648 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1935 5037 1935
Size: 3102 2231
BsaAI yAC'GTr
Cuts at: 2686 2686
Size: 5333
BsaBI GATnn'nnATC
Cuts at: 6 2847 4884 6
Size: 2841 2037 455
Fragments arranged by size:
2841 2037 455
BsaHI Gr'CG_yC
Cuts at: 1089 1185 1955 2764 3012 3714 5210 1089
Size: 96 770 809 248 702 1496 1212
Fragments arranged by size:
1496 1212 809 770 702 248 96
BsaWI w'CCGG_w
Cuts at: 1199 2522 3043 3721 3995 4142 4553 1199
Size: 1323 521 678 274 147 411 1979
Fragments arranged by size:
1979 1323 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 748 772 778 802 1016 1046 748
Size: 24 6 24 214 30 5035
Fragments arranged by size:
5035 214 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 1282 1444 1462 1465 1468 1471 1486 1489
Size: 1063 162 18 3 3 3 15 3
Cuts at: 1489 1510 1513 1558 1780 1903 1952 2240 219
Size: 21 3 45 222 123 49 288 3312
Fragments arranged by size:
3312 1063 288 222 162 123 49 45
21 18 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2107 2173 2758 3516 4093 4589 2107
Size: 66 585 758 577 496 2851
Fragments arranged by size:
2851 758 585 577 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 1142 483
Size: 659 4674
BsiEI CG_ry'CG
Cuts at: 104 858 2875 2921 4129 104
Size: 754 2017 46 1208 1308
Fragments arranged by size:
2017 1308 1208 754 46
BsiHKAI G_wGCw'C
Cuts at: 497 1296 2315 2347 3125 3315 4107 497
Size: 799 1019 32 778 190 792 1723
Fragments arranged by size:
1723 1019 799 792 778 190 32
BsmBI CGTCTCn'nnnn_
Cuts at: 1849 1962 1849
Size: 113 5220
Bsp1286I G_dGCh'C
Cuts at: 179 393 456 497 572 1296 1363 1390 1594
Size: 214 63 41 75 724 67 27 204
Cuts at: 1594 1895 2315 2347 2958 3051 3125 3315 3377
Size: 301 420 32 611 93 74 190 62
Cuts at: 3377 4107 179
Size: 730 1405
Fragments arranged by size:
1405 730 724 611 420 301 214 204
190 93 75 74 67 63 62 41
32 27
BspEI T'CCGG_A
Cuts at: 4553 4553
Size: 5333
BspHI T'CATG_A
Cuts at: 4509 4509
Size: 5333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 782 1561 2383 2597 2899 3280 782
Size: 779 822 214 302 381 2835
Fragments arranged by size:
2835 822 779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 924 3625 3679 5163 5328 50
Size: 874 2701 54 1484 165 55
Fragments arranged by size:
2701 1484 874 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1253 1755 3245 1253
Size: 502 1490 3341
Fragments arranged by size:
3341 1490 502
BsrFI r'CCGG_y
Cuts at: 377 461 853 3331 3512 3721 377
Size: 84 392 2478 181 209 1989
Fragments arranged by size:
2478 1989 392 209 181 84
BssSI C'ACGA_G
Cuts at: 2157 3604 3962 2157
Size: 1447 358 3528
Fragments arranged by size:
3528 1447 358
BstAPI GCAn_nnn'nTGC
Cuts at: 278 2710 278
Size: 2432 2901
BstBI TT'CG_AA
Cuts at: 308 3694 308
Size: 3386 1947
BstEII G'GTnAC_C
Cuts at: 2187 2187
Size: 5333
BstXI CCAn_nnnn'nTGG
Cuts at: 2163 2163
Size: 5333
BstYI r'GATC_y
Cuts at: 1 668 710 738 1167 1503 1610 1654 2358
Size: 667 42 28 429 336 107 44 704
Cuts at: 2358 2825 2848 3183 3429 4441 4527 4539 4885
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4885 1
Size: 449
Fragments arranged by size:
1012 704 667 467 449 429 346 336
335 246 107 86 44 42 28 23
12
Bsu36I CC'TnA_GG
Cuts at: 353 1763 353
Size: 1410 3923
BtgI C'CryG_G
Cuts at: 107 651 885 2178 107
Size: 544 234 1293 3262
Fragments arranged by size:
3262 1293 544 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3283 3464 3627 3283
Size: 181 163 4989
Fragments arranged by size:
4989 181 163
BtsI GCAGTG_nn'
Cuts at: 626 2576 626
Size: 1950 3383
DraI TTT'AAA
Cuts at: 2332 2332
Size: 5333
DrdI GACnn_nn'nnGTC
Cuts at: 3039 3897 3039
Size: 858 4475
EaeI y'GGCC_r
Cuts at: 110 463 855 1208 2279 2918 3092 3483 3510
Size: 353 392 353 1071 639 174 391 27
Cuts at: 3510 4855 110
Size: 1345 588
Fragments arranged by size:
1345 1071 639 588 392 391 353 353
174 27
EagI C'GGCC_G
Cuts at: 855 2918 855
Size: 2063 3270
EarI CTCTTCn'nnn_
Cuts at: 305 849 1284 1317 1439 1463 1487 1535 1547
Size: 544 435 33 122 24 24 48 12
Cuts at: 1547 1875 1938 2294 3356 3566 305
Size: 328 63 356 1062 210 2072
Fragments arranged by size:
2072 1062 544 435 356 328 210 122
63 48 33 24 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 430 796 3541 3851 3997 5143 430
Size: 366 2745 310 146 1146 620
Fragments arranged by size:
2745 1146 620 366 310 146
EcoICRI GAG'CTC
Cuts at: 495 1294 2345 495
Size: 799 1051 3483
Fragments arranged by size:
3483 1051 799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1018 1383 1511 1643 1713 1798 2198 3158
Size: 708 365 128 132 70 85 400 960
Cuts at: 3158 3590 4337 4605 310
Size: 432 747 268 1038
Fragments arranged by size:
1038 960 747 708 432 400 365 268
132 128 85 70
EcoNI CCTnn'n_nnAGG
Cuts at: 778 1872 2157 778
Size: 1094 285 3954
Fragments arranged by size:
3954 1094 285
EcoO109I rG'GnC_Cy
Cuts at: 175 176 441 647 943 1359 1360 2472 175
Size: 1 265 206 296 416 1 1112 3036
Fragments arranged by size:
3036 1112 416 296 265 206 1 1
EcoRI G'AATT_C
Cuts at: 2337 2337
Size: 5333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 334 555 3352 334
Size: 221 2797 2315
Fragments arranged by size:
2797 2315 221
FspI TGC'GCA
Cuts at: 3114 3114
Size: 5333
HaeII r_GCGC'y
Cuts at: 697 1188 2767 3015 4037 5016 697
Size: 491 1579 248 1022 979 1014
Fragments arranged by size:
1579 1022 1014 979 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 748 780 1016 1048 1079 1111 1852 1884 3175
Size: 32 236 32 31 32 741 32 1291
Cuts at: 3175 3205 3207 3237 748
Size: 30 2 30 2844
Fragments arranged by size:
2844 1291 741 236 32 32 32 32
31 30 30 2
HincII GTy'rAC
Cuts at: 909 1152 2372 909
Size: 243 1220 3870
Fragments arranged by size:
3870 1220 243
HindIII A'AGCT_T
Cuts at: 1823 1823
Size: 5333
HpaI GTT'AAC
Cuts at: 909 909
Size: 5333
KasI G'GCGC_C
Cuts at: 1184 2763 3011 1184
Size: 1579 248 3506
Fragments arranged by size:
3506 1579 248
KpnI G_GTAC'C
Cuts at: 2353 2353
Size: 5333
MluI A'CGCG_T
Cuts at: 4766 4766
Size: 5333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 716 4004 4188 716
Size: 3288 184 1861
Fragments arranged by size:
3288 1861 184
MscI TGG'CCA
Cuts at: 112 1210 2281 3094 112
Size: 1098 1071 813 2351
Fragments arranged by size:
2351 1098 1071 813
MslI CAynn'nnrTG
Cuts at: 130 2161 3322 3449 130
Size: 2031 1161 127 2014
Fragments arranged by size:
2031 2014 1161 127
MspA1I CmG'CkG
Cuts at: 163 687 997 2444 2758 3118 4131 4376 163
Size: 524 310 1447 314 360 1013 245 1120
Fragments arranged by size:
1447 1120 1013 524 360 314 310 245
NaeI GCC'GGC
Cuts at: 463 3514 463
Size: 3051 2282
NarI GG'CG_CC
Cuts at: 1185 2764 3012 1185
Size: 1579 248 3506
Fragments arranged by size:
3506 1579 248
NcoI C'CATG_G
Cuts at: 107 2178 107
Size: 2071 3262
NgoMIV G'CCGG_C
Cuts at: 461 3512 461
Size: 3051 2282
NspI r_CATG'y
Cuts at: 2386 2886 3793 2386
Size: 500 907 3926
Fragments arranged by size:
3926 907 500
PasI CC'CwG_GG
Cuts at: 1380 1380
Size: 5333
PciI A'CATG_T
Cuts at: 3789 3789
Size: 5333
PflMI CCAn_nnn'nTGG
Cuts at: 678 1041 2184 678
Size: 363 1143 3827
Fragments arranged by size:
3827 1143 363
PfoI T'CCnGG_A
Cuts at: 1873 1873
Size: 5333
PmeI GTTT'AAAC
Cuts at: 2332 2332
Size: 5333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 289 321 4472 4504 289
Size: 32 4151 32 1118
Fragments arranged by size:
4151 1118 32 32
PpuMI rG'GwC_Cy
Cuts at: 441 647 441
Size: 206 5127
PspOMI G'GGCC_C
Cuts at: 175 452 1359 175
Size: 277 907 4149
Fragments arranged by size:
4149 907 277
PstI C_TGCA'G
Cuts at: 725 779 1326 2380 3065 725
Size: 54 547 1054 685 2993
Fragments arranged by size:
2993 1054 685 547 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5333
PvuII CAG'CTG
Cuts at: 163 687 997 2758 3118 163
Size: 524 310 1761 360 2378
Fragments arranged by size:
2378 1761 524 360 310
RsrII CG'GwC_CG
Cuts at: 3528 3528
Size: 5333
SacI G_AGCT'C
Cuts at: 497 1296 2347 497
Size: 799 1051 3483
Fragments arranged by size:
3483 1051 799
SalI G'TCGA_C
Cuts at: 2370 2370
Size: 5333
SapI GCTCTTCn'nnn_
Cuts at: 849 1284 3356 3566 849
Size: 435 2072 210 2616
Fragments arranged by size:
2616 2072 435 210
SbfI CC_TGCA'GG
Cuts at: 779 2380 779
Size: 1601 3732
ScaI AGT'ACT
Cuts at: 4850 4850
Size: 5333
SfcI C'TryA_G
Cuts at: 33 721 775 983 1137 1322 1606 2376 3061
Size: 688 54 208 154 185 284 770 685
Cuts at: 3061 4054 4245 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 770 688 685 284 208 191
185 154 54
SfoI GGC'GCC
Cuts at: 1186 2765 3013 1186
Size: 1579 248 3506
Fragments arranged by size:
3506 1579 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
SmaI CCC'GGG
Cuts at: 2355 2355
Size: 5333
SmlI C'TyrA_G
Cuts at: 508 2488 3403 3895 4157 508
Size: 1980 915 492 262 1684
Fragments arranged by size:
1980 1684 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5333
SphI G_CATG'C
Cuts at: 2386 2886 2386
Size: 500 4833
StyI C'CwwG_G
Cuts at: 107 602 643 1011 1302 1343 2123 2132 2178
Size: 495 41 368 291 41 780 9 46
Cuts at: 2178 2467 2821 107
Size: 289 354 2619
Fragments arranged by size:
2619 780 495 368 354 291 289 46
41 41 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 200 2916 3049 3508 3717 200
Size: 2716 133 459 209 1816
Fragments arranged by size:
2716 1816 459 209 133
TatI w'GTAC_w
Cuts at: 4848 4848
Size: 5333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1673 1705 4850 4882 1673
Size: 32 3145 32 2124
Fragments arranged by size:
3145 2124 32 32
Tth111I GACn'n_nGTC
Cuts at: 3130 3130
Size: 5333
XbaI T'CTAG_A
Cuts at: 2364 2364
Size: 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1218 2181 2828 1218
Size: 963 647 3723
Fragments arranged by size:
3723 963 647
XmaI C'CCGG_G
Cuts at: 2353 2353
Size: 5333
XmnI GAAnn'nnTTC
Cuts at: 304 304
Size: 5333
ZraI GAC'GTC
Cuts at: 1090 1090
Size: 5333
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI