Restriction map for
pF1KB6232
(Circular) (Six-base) MAPSORT of: pF1KB6232.seq Check: 4071 from: 1 to: 5141
pF1KB6232 5141 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2179 2179
Size: 5141
Acc65I G'GTAC_C
Cuts at: 2157 2157
Size: 5141
AclI AA'CG_TT
Cuts at: 4981 4981
Size: 5141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3398 4145 4413 2966
Size: 432 747 268 3694
Fragments arranged by size:
3694 747 432 268
AfeI AGC'GCT
Cuts at: 1043 1043
Size: 5141
AflIII A'CryG_T
Cuts at: 738 2493 3597 4574 738
Size: 1755 1104 977 1305
Fragments arranged by size:
1755 1305 1104 977
AgeI A'CCGG_T
Cuts at: 3529 3529
Size: 5141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 482 1273 1448 482
Size: 791 175 4175
Fragments arranged by size:
4175 791 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1048 1080 1048
Size: 32 5109
AlwNI CAG_nnn'CTG
Cuts at: 370 2031 2446 4013 370
Size: 1661 415 1567 1498
Fragments arranged by size:
1661 1567 1498 415
ApaI G_GGCC'C
Cuts at: 123 170 224 1287 123
Size: 47 54 1063 3977
Fragments arranged by size:
3977 1063 54 47
ApaLI G'TGCA_C
Cuts at: 753 1757 3911 753
Size: 1004 2154 1983
Fragments arranged by size:
2154 1983 1004
ApoI r'AATT_y
Cuts at: 2145 4494 4750 2145
Size: 2349 256 2536
Fragments arranged by size:
2536 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5141
AvaI C'yCGr_G
Cuts at: 163 250 698 1022 1120 1225 1504 1990 2161
Size: 87 448 324 98 105 279 486 171
Cuts at: 2161 163
Size: 3143
Fragments arranged by size:
3143 486 448 324 279 171 105 98
87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 727 760 727
Size: 33 5108
BamHI G'GATC_C
Cuts at: 2166 2166
Size: 5141
BanI G'GyrC_C
Cuts at: 172 375 694 2094 2157 2571 2819 2854 172
Size: 203 319 1400 63 414 248 35 2459
Fragments arranged by size:
2459 1400 414 319 248 203 63 35
BanII G_rGCy'C
Cuts at: 123 161 170 224 625 955 1287 1432 1646
Size: 38 9 54 401 330 332 145 214
Cuts at: 1646 2155 3185 123
Size: 509 1030 2079
Fragments arranged by size:
2079 1030 509 401 332 330 214 145
54 38 9
BbeI G_GCGC'C
Cuts at: 176 2098 2575 2823 176
Size: 1922 477 248 2494
Fragments arranged by size:
2494 1922 477 248
BbvCI CC'TCA_GC
Cuts at: 133 2114 133
Size: 1981 3160
BciVI GTATCCnnnnn_n'
Cuts at: 3039 3800 5141 3039
Size: 761 1341 3039
Fragments arranged by size:
3039 1341 761
BclI T'GATC_A
Cuts at: 2661 2661
Size: 5141
BglI GCCn_nnn'nGGC
Cuts at: 165 1282 1905 165
Size: 1117 623 3401
Fragments arranged by size:
3401 1117 623
BglII A'GATC_T
Cuts at: 2656 4693 2656
Size: 2037 3104
BlpI GC'TnA_GC
Cuts at: 884 982 1393 2253 884
Size: 98 411 860 3772
Fragments arranged by size:
3772 860 411 98
Bme1580I G_kGCm'C
Cuts at: 123 170 224 452 699 757 1287 1761 2766
Size: 47 54 228 247 58 530 474 1005
Cuts at: 2766 2859 3915 123
Size: 93 1056 1349
Fragments arranged by size:
1349 1056 1005 530 474 247 228 93
58 54 47
BmgBI CAC'GTC
Cuts at: 1830 1830
Size: 5141
BmrI ACTGGGnnnn_n'
Cuts at: 599 2353 2434 2769 4913 599
Size: 1754 81 335 2144 827
Fragments arranged by size:
2144 1754 827 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1446 3508 1446
Size: 2062 3079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 928 1189 1219 1505 1684 2110 609
Size: 319 261 30 286 179 426 3640
Fragments arranged by size:
3640 426 319 286 261 179 30
Bpu10I CC'TnA_GC
Cuts at: 133 619 1591 2114 133
Size: 486 972 523 3160
Fragments arranged by size:
3160 972 523 486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 2317 3196 3688 3986 337
Size: 1980 879 492 298 1492
Fragments arranged by size:
1980 1492 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1019 4845 1019
Size: 3826 1315
BsaAI yAC'GTr
Cuts at: 645 2494 645
Size: 1849 3292
BsaBI GATnn'nnATC
Cuts at: 6 557 2655 4692 6
Size: 551 2098 2037 455
Fragments arranged by size:
2098 2037 551 455
BsaHI Gr'CG_yC
Cuts at: 173 246 272 1529 1700 2095 2572 2820 3522
Size: 73 26 1257 171 395 477 248 702
Cuts at: 3522 5018 173
Size: 1496 296
Fragments arranged by size:
1496 1257 702 477 395 296 248 171
73 26
BsaWI w'CCGG_w
Cuts at: 125 482 1949 2330 2851 3529 3803 3950 4361
Size: 357 1467 381 521 678 274 147 411
Cuts at: 4361 125
Size: 905
Fragments arranged by size:
1467 905 678 521 411 381 357 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2113 2143 2113
Size: 30 5111
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 883 1681 883
Size: 798 4343
BseYI C'CCAG_C
Cuts at: 609 1346 1710 2005 2566 3324 3901 4397 609
Size: 737 364 295 561 758 577 496 1353
Fragments arranged by size:
1353 758 737 577 561 496 364 295
BsiEI CG_ry'CG
Cuts at: 104 561 2683 2729 3937 104
Size: 457 2122 46 1208 1308
Fragments arranged by size:
2122 1308 1208 457 46
BsiHKAI G_wGCw'C
Cuts at: 625 653 757 863 1432 1748 1761 2155 2933
Size: 28 104 106 569 316 13 394 778
Cuts at: 2933 3123 3915 625
Size: 190 792 1851
Fragments arranged by size:
1851 792 778 569 394 316 190 106
104 28 13
Bsp1286I G_dGCh'C
Cuts at: 123 161 170 224 452 625 653 699 757
Size: 38 9 54 228 173 28 46 58
Cuts at: 757 863 955 1287 1432 1646 1748 1761 2155
Size: 106 92 332 145 214 102 13 394
Cuts at: 2155 2766 2859 2933 3123 3185 3915 123
Size: 611 93 74 190 62 730 1349
Fragments arranged by size:
1349 730 611 394 332 228 214 190
173 145 106 102 93 92 74 62
58 54 46 38 28 13 9
BspEI T'CCGG_A
Cuts at: 125 482 4361 125
Size: 357 3879 905
Fragments arranged by size:
3879 905 357
BspHI T'CATG_A
Cuts at: 4317 4317
Size: 5141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2191 2405 2707 3088 2191
Size: 214 302 381 4244
Fragments arranged by size:
4244 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3433 3487 4971 5136 50
Size: 3383 54 1484 165 55
Fragments arranged by size:
3383 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1934 3053 1934
Size: 1119 4022
BsrFI r'CCGG_y
Cuts at: 1100 1733 3139 3320 3529 1100
Size: 633 1406 181 209 2712
Fragments arranged by size:
2712 1406 633 209 181
BsrGI T'GTAC_A
Cuts at: 1046 1046
Size: 5141
BssSI C'ACGA_G
Cuts at: 1678 2101 3412 3770 1678
Size: 423 1311 358 3049
Fragments arranged by size:
3049 1311 423 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2518 2518
Size: 5141
BstBI TT'CG_AA
Cuts at: 3502 3502
Size: 5141
BstYI r'GATC_y
Cuts at: 1 2166 2633 2656 2991 3237 4249 4335 4347
Size: 2165 467 23 335 246 1012 86 12
Cuts at: 4347 4693 1
Size: 346 449
Fragments arranged by size:
2165 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 724 905 1715 1923 1973 724
Size: 181 810 208 50 3892
Fragments arranged by size:
3892 810 208 181 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1001 3091 3272 3435 1001
Size: 2090 181 163 2707
Fragments arranged by size:
2707 2090 181 163
BtsI GCAGTG_nn'
Cuts at: 1521 1700 2384 1521
Size: 179 684 4278
Fragments arranged by size:
4278 684 179
ClaI AT'CG_AT
Cuts at: 557 557
Size: 5141
DraI TTT'AAA
Cuts at: 2140 2140
Size: 5141
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2847 3705 1839
Size: 1008 858 3275
Fragments arranged by size:
3275 1008 858
EaeI y'GGCC_r
Cuts at: 150 902 2066 2726 2900 3291 3318 4663 150
Size: 752 1164 660 174 391 27 1345 628
Fragments arranged by size:
1345 1164 752 660 628 391 174 27
EagI C'GGCC_G
Cuts at: 2726 2726
Size: 5141
EarI CTCTTCn'nnn_
Cuts at: 1318 1948 3164 3374 1318
Size: 630 1216 210 3085
Fragments arranged by size:
3085 1216 630 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1297 1752 3349 3659 3805 4951 1297
Size: 455 1597 310 146 1146 1487
Fragments arranged by size:
1597 1487 1146 455 310 146
EcoICRI GAG'CTC
Cuts at: 623 1430 2153 623
Size: 807 723 3611
Fragments arranged by size:
3611 807 723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 928 1189 1219 1505 1684 2110 2966 3398
Size: 319 261 30 286 179 426 856 432
Cuts at: 3398 4145 4413 609
Size: 747 268 1337
Fragments arranged by size:
1337 856 747 432 426 319 286 268
261 179 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1315 1893 1315
Size: 578 4563
EcoO109I rG'GnC_Cy
Cuts at: 120 220 1094 1239 1707 2028 2280 120
Size: 100 874 145 468 321 252 2981
Fragments arranged by size:
2981 874 468 321 252 145 100
EcoRI G'AATT_C
Cuts at: 2145 2145
Size: 5141
EcoRV GAT'ATC
Cuts at: 555 555
Size: 5141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 5141
FspI TGC'GCA
Cuts at: 2922 2922
Size: 5141
HaeII r_GCGC'y
Cuts at: 176 1045 1198 1471 2098 2575 2823 3845 4824
Size: 869 153 273 627 477 248 1022 979
Cuts at: 4824 176
Size: 493
Fragments arranged by size:
1022 979 869 627 493 477 273 248
153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1896 1928 2983 3013 3015 3045 1896
Size: 32 1055 30 2 30 3992
Fragments arranged by size:
3992 1055 32 30 30 2
HincII GTy'rAC
Cuts at: 282 966 2180 282
Size: 684 1214 3243
Fragments arranged by size:
3243 1214 684
KasI G'GCGC_C
Cuts at: 172 2094 2571 2819 172
Size: 1922 477 248 2494
Fragments arranged by size:
2494 1922 477 248
KpnI G_GTAC'C
Cuts at: 2161 2161
Size: 5141
MluI A'CGCG_T
Cuts at: 4574 4574
Size: 5141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3812 3996 3812
Size: 184 4957
MscI TGG'CCA
Cuts at: 2068 2902 2068
Size: 834 4307
MslI CAynn'nnrTG
Cuts at: 470 1778 3130 3257 470
Size: 1308 1352 127 2354
Fragments arranged by size:
2354 1352 1308 127
MspA1I CmG'CkG
Cuts at: 1599 2252 2566 2926 3939 4184 1599
Size: 653 314 360 1013 245 2556
Fragments arranged by size:
2556 1013 653 360 314 245
NaeI GCC'GGC
Cuts at: 1102 3322 1102
Size: 2220 2921
NarI GG'CG_CC
Cuts at: 173 2095 2572 2820 173
Size: 1922 477 248 2494
Fragments arranged by size:
2494 1922 477 248
NcoI C'CATG_G
Cuts at: 1923 1973 1923
Size: 50 5091
NgoMIV G'CCGG_C
Cuts at: 1100 3320 1100
Size: 2220 2921
NspI r_CATG'y
Cuts at: 1254 2194 2694 3601 1254
Size: 940 500 907 2794
Fragments arranged by size:
2794 940 907 500
PciI A'CATG_T
Cuts at: 3597 3597
Size: 5141
PflMI CCAn_nnn'nTGG
Cuts at: 1658 1658
Size: 5141
PfoI T'CCnGG_A
Cuts at: 625 1166 1316 625
Size: 541 150 4450
Fragments arranged by size:
4450 541 150
PmeI GTTT'AAAC
Cuts at: 2140 2140
Size: 5141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 4280
Size: 32 5109
PpuMI rG'GwC_Cy
Cuts at: 1239 1707 1239
Size: 468 4673
PspOMI G'GGCC_C
Cuts at: 119 166 220 1283 119
Size: 47 54 1063 3977
Fragments arranged by size:
3977 1063 54 47
PspXI vC'TCGA_Gb
Cuts at: 1504 1504
Size: 5141
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1282 1314 1282
Size: 32 5109
PstI C_TGCA'G
Cuts at: 980 2188 2873 980
Size: 1208 685 3248
Fragments arranged by size:
3248 1208 685
PvuI CG_AT'CG
Cuts at: 104 561 104
Size: 457 4684
PvuII CAG'CTG
Cuts at: 1599 2566 2926 1599
Size: 967 360 3814
Fragments arranged by size:
3814 967 360
RsrII CG'GwC_CG
Cuts at: 3336 3336
Size: 5141
SacI G_AGCT'C
Cuts at: 625 1432 2155 625
Size: 807 723 3611
Fragments arranged by size:
3611 807 723
SalI G'TCGA_C
Cuts at: 2178 2178
Size: 5141
SanDI GG'GwC_CC
Cuts at: 1707 1707
Size: 5141
SapI GCTCTTCn'nnn_
Cuts at: 3164 3374 3164
Size: 210 4931
SbfI CC_TGCA'GG
Cuts at: 2188 2188
Size: 5141
ScaI AGT'ACT
Cuts at: 1204 4658 1204
Size: 3454 1687
SexAI A'CCwGG_T
Cuts at: 801 801
Size: 5141
SfcI C'TryA_G
Cuts at: 33 792 976 2184 2869 3862 4053 33
Size: 759 184 1208 685 993 191 1121
Fragments arranged by size:
1208 1121 993 759 685 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 1282 1282
Size: 5141
SfoI GGC'GCC
Cuts at: 174 2096 2573 2821 174
Size: 1922 477 248 2494
Fragments arranged by size:
2494 1922 477 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5141
SmaI CCC'GGG
Cuts at: 252 700 2163 252
Size: 448 1463 3230
Fragments arranged by size:
3230 1463 448
SmlI C'TyrA_G
Cuts at: 316 1022 1225 1504 2296 3211 3703 3965 316
Size: 706 203 279 792 915 492 262 1492
Fragments arranged by size:
1492 915 792 706 492 279 262 203
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5141
SphI G_CATG'C
Cuts at: 1254 2194 2694 1254
Size: 940 500 3701
Fragments arranged by size:
3701 940 500
SrfI GCCC'GGGC
Cuts at: 700 700
Size: 5141
StuI AGG'CCT
Cuts at: 839 1447 839
Size: 608 4533
StyI C'CwwG_G
Cuts at: 1614 1923 1973 2275 2629 1614
Size: 309 50 302 354 4126
Fragments arranged by size:
4126 354 309 302 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 2724 2857 3316 3525 1776
Size: 948 133 459 209 3392
Fragments arranged by size:
3392 948 459 209 133
TatI w'GTAC_w
Cuts at: 1046 1202 4656 1046
Size: 156 3454 1531
Fragments arranged by size:
3454 1531 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2113 2145 4658 4690 2113
Size: 32 2513 32 2564
Fragments arranged by size:
2564 2513 32 32
Tth111I GACn'n_nGTC
Cuts at: 2938 2938
Size: 5141
XbaI T'CTAG_A
Cuts at: 2172 2172
Size: 5141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 819 1659 2636 819
Size: 840 977 3324
Fragments arranged by size:
3324 977 840
XhoI C'TCGA_G
Cuts at: 1022 1225 1504 1022
Size: 203 279 4659
Fragments arranged by size:
4659 279 203
XmaI C'CCGG_G
Cuts at: 250 698 2161 250
Size: 448 1463 3230
Fragments arranged by size:
3230 1463 448
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BbsI
BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI SacII SgrAI SnaBI
SspI SwaI XmnI ZraI