Restriction map for
pF1KB6236
(Circular) (Six-base) MAPSORT of: pF1KB6236.seq Check: 1528 from: 1 to: 5141
pF1KB6236 5141 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 277 2179 277
Size: 1902 3239
Acc65I G'GTAC_C
Cuts at: 2157 2157
Size: 5141
AclI AA'CG_TT
Cuts at: 4981 4981
Size: 5141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 1696 1950 2966 3398 4145 4413 1231
Size: 465 254 1016 432 747 268 1959
Fragments arranged by size:
1959 1016 747 465 432 268 254
AfeI AGC'GCT
Cuts at: 388 388
Size: 5141
AflIII A'CryG_T
Cuts at: 2493 3597 4574 2493
Size: 1104 977 3060
Fragments arranged by size:
3060 1104 977
AgeI A'CCGG_T
Cuts at: 3529 3529
Size: 5141
AhdI GACnn_n'nnGTC
Cuts at: 1701 1701
Size: 5141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 925 925
Size: 5141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1360 1392 1360
Size: 32 5109
AlwNI CAG_nnn'CTG
Cuts at: 352 1033 1674 2446 4013 352
Size: 681 641 772 1567 1480
Fragments arranged by size:
1567 1480 772 681 641
ApaLI G'TGCA_C
Cuts at: 910 1284 3911 910
Size: 374 2627 2140
Fragments arranged by size:
2627 2140 374
ApoI r'AATT_y
Cuts at: 2113 2145 4494 4750 2113
Size: 32 2349 256 2504
Fragments arranged by size:
2504 2349 256 32
AscI GG'CGCG_CC
Cuts at: 2032 2032
Size: 5141
AseI AT'TA_AT
Cuts at: 20 20
Size: 5141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5141
AvaI C'yCGr_G
Cuts at: 2161 2161
Size: 5141
BamHI G'GATC_C
Cuts at: 2166 2166
Size: 5141
BanI G'GyrC_C
Cuts at: 111 736 1636 2157 2571 2819 2854 111
Size: 625 900 521 414 248 35 2398
Fragments arranged by size:
2398 900 625 521 414 248 35
BanII G_rGCy'C
Cuts at: 154 829 2155 3185 154
Size: 675 1326 1030 2110
Fragments arranged by size:
2110 1326 1030 675
BbeI G_GCGC'C
Cuts at: 115 2575 2823 115
Size: 2460 248 2433
Fragments arranged by size:
2460 2433 248
BbsI GAAGACnn'nnnn_
Cuts at: 899 1069 899
Size: 170 4971
BbvCI CC'TCA_GC
Cuts at: 971 971
Size: 5141
BciVI GTATCCnnnnn_n'
Cuts at: 272 3039 3800 5141 272
Size: 2767 761 1341 272
Fragments arranged by size:
2767 1341 761 272
BclI T'GATC_A
Cuts at: 2661 2661
Size: 5141
BfrBI ATG'CAT
Cuts at: 707 707
Size: 5141
BglI GCCn_nnn'nGGC
Cuts at: 1240 1240
Size: 5141
BglII A'GATC_T
Cuts at: 1115 2656 4693 1115
Size: 1541 2037 1563
Fragments arranged by size:
2037 1563 1541
BlpI GC'TnA_GC
Cuts at: 214 2253 214
Size: 2039 3102
Bme1580I G_kGCm'C
Cuts at: 914 1288 2766 2859 3915 914
Size: 374 1478 93 1056 2140
Fragments arranged by size:
2140 1478 1056 374 93
BmgBI CAC'GTC
Cuts at: 480 1278 480
Size: 798 4343
BmrI ACTGGGnnnn_n'
Cuts at: 2353 2434 2769 4913 2353
Size: 81 335 2144 2581
Fragments arranged by size:
2581 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 3508 168
Size: 3340 1801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1864 493
Size: 1371 3770
Bpu10I CC'TnA_GC
Cuts at: 971 971
Size: 5141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2317 3196 3688 3986 1231
Size: 1086 879 492 298 2386
Fragments arranged by size:
2386 1086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4845 4845
Size: 5141
BsaAI yAC'GTr
Cuts at: 2494 2494
Size: 5141
BsaBI GATnn'nnATC
Cuts at: 6 2655 4692 6
Size: 2649 2037 455
Fragments arranged by size:
2649 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 287 2572 2820 3522 5018 112
Size: 175 2285 248 702 1496 235
Fragments arranged by size:
2285 1496 702 248 235 175
BsaWI w'CCGG_w
Cuts at: 1066 2330 2851 3529 3803 3950 4361 1066
Size: 1264 521 678 274 147 411 1846
Fragments arranged by size:
1846 1264 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 1006 1036 334
Size: 30 642 30 4439
Fragments arranged by size:
4439 642 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 1014 1235 355
Size: 659 221 4261
Fragments arranged by size:
4261 659 221
BseYI C'CCAG_C
Cuts at: 147 1075 1878 2566 3324 3901 4397 147
Size: 928 803 688 758 577 496 891
Fragments arranged by size:
928 891 803 758 688 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1335 262
Size: 1073 4068
BsiEI CG_ry'CG
Cuts at: 104 2683 2729 3937 104
Size: 2579 46 1208 1308
Fragments arranged by size:
2579 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 914 1288 2123 2155 2933 3123 3915 914
Size: 374 835 32 778 190 792 2140
Fragments arranged by size:
2140 835 792 778 374 190 32
BsmI GAATG_Cn'
Cuts at: 2083 2083
Size: 5141
Bsp1286I G_dGCh'C
Cuts at: 154 829 914 1288 2123 2155 2766 2859 2933
Size: 675 85 374 835 32 611 93 74
Cuts at: 2933 3123 3185 3915 154
Size: 190 62 730 1380
Fragments arranged by size:
1380 835 730 675 611 374 190 93
85 74 62 32
BspEI T'CCGG_A
Cuts at: 4361 4361
Size: 5141
BspHI T'CATG_A
Cuts at: 4317 4317
Size: 5141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 2191 2405 2707 3088 437
Size: 1754 214 302 381 2490
Fragments arranged by size:
2490 1754 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3433 3487 4971 5136 50
Size: 3383 54 1484 165 55
Fragments arranged by size:
3383 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 685 3053 685
Size: 2368 2773
BsrFI r'CCGG_y
Cuts at: 3139 3320 3529 3139
Size: 181 209 4751
Fragments arranged by size:
4751 209 181
BssHII G'CGCG_C
Cuts at: 2032 2032
Size: 5141
BssSI C'ACGA_G
Cuts at: 3412 3770 3412
Size: 358 4783
BstAPI GCAn_nnn'nTGC
Cuts at: 1136 1631 2518 1136
Size: 495 887 3759
Fragments arranged by size:
3759 887 495
BstBI TT'CG_AA
Cuts at: 3502 3502
Size: 5141
BstEII G'GTnAC_C
Cuts at: 357 357
Size: 5141
BstXI CCAn_nnnn'nTGG
Cuts at: 1791 1878 1791
Size: 87 5054
BstYI r'GATC_y
Cuts at: 1 1115 1661 2166 2633 2656 2991 3237 4249
Size: 1114 546 505 467 23 335 246 1012
Cuts at: 4249 4335 4347 4693 1
Size: 86 12 346 449
Fragments arranged by size:
1114 1012 546 505 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 437 437
Size: 5141
BtgI C'CryG_G
Cuts at: 107 362 540 740 1289 1752 2069 107
Size: 255 178 200 549 463 317 3179
Fragments arranged by size:
3179 549 463 317 255 200 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 111 3091 3272 3435 111
Size: 2980 181 163 1817
Fragments arranged by size:
2980 1817 181 163
BtsI GCAGTG_nn'
Cuts at: 510 843 2384 510
Size: 333 1541 3267
Fragments arranged by size:
3267 1541 333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 585 620 585
Size: 35 5106
DraI TTT'AAA
Cuts at: 2140 2140
Size: 5141
DrdI GACnn_nn'nnGTC
Cuts at: 2847 3705 2847
Size: 858 4283
EaeI y'GGCC_r
Cuts at: 380 743 935 1560 1737 2726 2900 3291 3318
Size: 363 192 625 177 989 174 391 27
Cuts at: 3318 4663 380
Size: 1345 858
Fragments arranged by size:
1345 989 858 625 391 363 192 177
174 27
EagI C'GGCC_G
Cuts at: 2726 2726
Size: 5141
EarI CTCTTCn'nnn_
Cuts at: 1780 3164 3374 1780
Size: 1384 210 3547
Fragments arranged by size:
3547 1384 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3349 3659 3805 4951 3349
Size: 310 146 1146 3539
Fragments arranged by size:
3539 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2153 2153
Size: 5141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1231 1696 1864 1950 2966 3398 4145 4413
Size: 738 465 168 86 1016 432 747 268
Cuts at: 4413 493
Size: 1221
Fragments arranged by size:
1221 1016 747 738 465 432 268 168
86
EcoNI CCTnn'n_nnAGG
Cuts at: 1249 1249
Size: 5141
EcoO109I rG'GnC_Cy
Cuts at: 648 1103 1167 1242 1407 1524 1671 1713 2280
Size: 455 64 75 165 117 147 42 567
Cuts at: 2280 648
Size: 3509
Fragments arranged by size:
3509 567 455 165 147 117 75 64
42
EcoRI G'AATT_C
Cuts at: 2145 2145
Size: 5141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 5141
FspI TGC'GCA
Cuts at: 124 502 2922 124
Size: 378 2420 2343
Fragments arranged by size:
2420 2343 378
HaeII r_GCGC'y
Cuts at: 115 214 390 2575 2823 3845 4824 115
Size: 99 176 2185 248 1022 979 432
Fragments arranged by size:
2185 1022 979 432 248 176 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 259 278 291 334 366 1006 1038 1687
Size: 13 19 13 43 32 640 32 649
Cuts at: 1687 1719 2983 3013 3015 3045 246
Size: 32 1264 30 2 30 2342
Fragments arranged by size:
2342 1264 649 640 43 32 32 32
30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 278 2180 278
Size: 1902 3239
HindIII A'AGCT_T
Cuts at: 961 961
Size: 5141
KasI G'GCGC_C
Cuts at: 111 2571 2819 111
Size: 2460 248 2433
Fragments arranged by size:
2460 2433 248
KpnI G_GTAC'C
Cuts at: 2161 2161
Size: 5141
MluI A'CGCG_T
Cuts at: 4574 4574
Size: 5141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1529 1934 3812 3996 1529
Size: 405 1878 184 2674
Fragments arranged by size:
2674 1878 405 184
MscI TGG'CCA
Cuts at: 937 1562 1739 2902 937
Size: 625 177 1163 3176
Fragments arranged by size:
3176 1163 625 177
MslI CAynn'nnrTG
Cuts at: 296 790 907 1757 1789 3130 3257 296
Size: 494 117 850 32 1341 127 2180
Fragments arranged by size:
2180 1341 850 494 127 117 32
MspA1I CmG'CkG
Cuts at: 542 742 1471 1814 2252 2566 2926 3939 4184
Size: 200 729 343 438 314 360 1013 245
Cuts at: 4184 542
Size: 1499
Fragments arranged by size:
1499 1013 729 438 360 343 314 245
200
NaeI GCC'GGC
Cuts at: 3322 3322
Size: 5141
NarI GG'CG_CC
Cuts at: 112 2572 2820 112
Size: 2460 248 2433
Fragments arranged by size:
2460 2433 248
NcoI C'CATG_G
Cuts at: 107 1289 1752 2069 107
Size: 1182 463 317 3179
Fragments arranged by size:
3179 1182 463 317
NgoMIV G'CCGG_C
Cuts at: 3320 3320
Size: 5141
NsiI A_TGCA'T
Cuts at: 709 709
Size: 5141
NspI r_CATG'y
Cuts at: 795 1089 2194 2694 3601 795
Size: 294 1105 500 907 2335
Fragments arranged by size:
2335 1105 907 500 294
PciI A'CATG_T
Cuts at: 3597 3597
Size: 5141
PflMI CCAn_nnn'nTGG
Cuts at: 1928 1928
Size: 5141
PfoI T'CCnGG_A
Cuts at: 470 1003 1424 1841 2126 470
Size: 533 421 417 285 3485
Fragments arranged by size:
3485 533 421 417 285
PmeI GTTT'AAAC
Cuts at: 2140 2140
Size: 5141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 505 537 4280 4312 505
Size: 32 3743 32 1334
Fragments arranged by size:
3743 1334 32 32
PpuMI rG'GwC_Cy
Cuts at: 1103 1167 1407 1524 1713 1103
Size: 64 240 117 189 4531
Fragments arranged by size:
4531 240 189 117 64
PstI C_TGCA'G
Cuts at: 161 434 1493 2188 2873 161
Size: 273 1059 695 685 2429
Fragments arranged by size:
2429 1059 695 685 273
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5141
PvuII CAG'CTG
Cuts at: 2566 2926 2566
Size: 360 4781
RsrII CG'GwC_CG
Cuts at: 3336 3336
Size: 5141
SacI G_AGCT'C
Cuts at: 2155 2155
Size: 5141
SacII CC_GC'GG
Cuts at: 543 743 543
Size: 200 4941
SalI G'TCGA_C
Cuts at: 276 2178 276
Size: 1902 3239
SapI GCTCTTCn'nnn_
Cuts at: 3164 3374 3164
Size: 210 4931
SbfI CC_TGCA'GG
Cuts at: 161 2188 161
Size: 2027 3114
ScaI AGT'ACT
Cuts at: 1228 4658 1228
Size: 3430 1711
SfcI C'TryA_G
Cuts at: 33 157 430 1489 1666 1995 2043 2184 2869
Size: 124 273 1059 177 329 48 141 685
Cuts at: 2869 3862 4053 33
Size: 993 191 1121
Fragments arranged by size:
1121 1059 993 685 329 273 191 177
141 124 48
SfoI GGC'GCC
Cuts at: 113 2573 2821 113
Size: 2460 248 2433
Fragments arranged by size:
2460 2433 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5141
SmaI CCC'GGG
Cuts at: 2163 2163
Size: 5141
SmlI C'TyrA_G
Cuts at: 1246 2296 3211 3703 3965 1246
Size: 1050 915 492 262 2422
Fragments arranged by size:
2422 1050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5141
SphI G_CATG'C
Cuts at: 2194 2694 2194
Size: 500 4641
StuI AGG'CCT
Cuts at: 976 976
Size: 5141
StyI C'CwwG_G
Cuts at: 107 443 1289 1364 1752 1823 1832 2069 2275
Size: 336 846 75 388 71 9 237 206
Cuts at: 2275 2629 107
Size: 354 2619
Fragments arranged by size:
2619 846 388 354 336 237 206 75
71 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 1006 1354 2001 2724 2857 3316 3525 355
Size: 651 348 647 723 133 459 209 1971
Fragments arranged by size:
1971 723 651 647 459 348 209 133
TatI w'GTAC_w
Cuts at: 1226 1679 4656 1226
Size: 453 2977 1711
Fragments arranged by size:
2977 1711 453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4658 4690 4658
Size: 32 5109
Tth111I GACn'n_nGTC
Cuts at: 640 2938 640
Size: 2298 2843
XbaI T'CTAG_A
Cuts at: 2172 2172
Size: 5141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1839 2636 1839
Size: 797 4344
XmaI C'CCGG_G
Cuts at: 2161 2161
Size: 5141
XmnI GAAnn'nnTTC
Cuts at: 1603 1603
Size: 5141
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI ApaI AvrII BaeI BcgI
BmtI BsiWI BsmBI BsrGI BstZ17I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI