Restriction map for
pF1KB6240
(Circular) (Six-base) MAPSORT of: pF1KB6240.seq Check: 1123 from: 1 to: 5144
pF1KB6240 5144 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 615 1220 615
Size: 605 4539
AccI GT'mk_AC
Cuts at: 1225 1851 2182 1225
Size: 626 331 4187
Fragments arranged by size:
4187 626 331
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 1691 4984 1691
Size: 3293 1851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 301 370 2063 2969 3401 4148 4416 277
Size: 24 69 1693 906 432 747 268 1005
Fragments arranged by size:
1693 1005 906 747 432 268 69 24
AflIII A'CryG_T
Cuts at: 877 2007 2496 3600 4577 877
Size: 1130 489 1104 977 1444
Fragments arranged by size:
1444 1130 1104 977 489
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AlwNI CAG_nnn'CTG
Cuts at: 2449 4016 2449
Size: 1567 3577
ApaI G_GGCC'C
Cuts at: 2051 2051
Size: 5144
ApaLI G'TGCA_C
Cuts at: 3914 3914
Size: 5144
ApoI r'AATT_y
Cuts at: 1477 1726 2148 4497 4753 1477
Size: 249 422 2349 256 1868
Fragments arranged by size:
2349 1868 422 256 249
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 733 2164 733
Size: 1431 3713
BamHI G'GATC_C
Cuts at: 2169 2169
Size: 5144
BanI G'GyrC_C
Cuts at: 2160 2574 2822 2857 2160
Size: 414 248 35 4447
Fragments arranged by size:
4447 414 248 35
BanII G_rGCy'C
Cuts at: 475 2024 2051 2158 3188 475
Size: 1549 27 107 1030 2431
Fragments arranged by size:
2431 1549 1030 107 27
BbeI G_GCGC'C
Cuts at: 2578 2826 2578
Size: 248 4896
BbsI GAAGACnn'nnnn_
Cuts at: 193 1654 193
Size: 1461 3683
BbvCI CC'TCA_GC
Cuts at: 476 1247 476
Size: 771 4373
BciVI GTATCCnnnnn_n'
Cuts at: 3042 3803 5144 3042
Size: 761 1341 3042
Fragments arranged by size:
3042 1341 761
BclI T'GATC_A
Cuts at: 2664 2664
Size: 5144
BfrBI ATG'CAT
Cuts at: 1519 1519
Size: 5144
BglII A'GATC_T
Cuts at: 1577 1978 2659 4696 1577
Size: 401 681 2037 2025
Fragments arranged by size:
2037 2025 681 401
BlpI GC'TnA_GC
Cuts at: 469 2256 469
Size: 1787 3357
Bme1580I G_kGCm'C
Cuts at: 1445 2051 2769 2862 3918 1445
Size: 606 718 93 1056 2671
Fragments arranged by size:
2671 1056 718 606 93
BmgBI CAC'GTC
Cuts at: 2010 2010
Size: 5144
BmrI ACTGGGnnnn_n'
Cuts at: 2356 2437 2772 4916 2356
Size: 81 335 2144 2584
Fragments arranged by size:
2584 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1334 3511 1334
Size: 2177 2967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 469 481 1535 439
Size: 30 12 1054 4048
Fragments arranged by size:
4048 1054 30 12
Bpu10I CC'TnA_GC
Cuts at: 476 1247 476
Size: 771 4373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 752 2320 3199 3691 3989 404
Size: 348 1568 879 492 298 1559
Fragments arranged by size:
1568 1559 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 4848 4848
Size: 5144
BsaAI yAC'GTr
Cuts at: 1631 2497 1631
Size: 866 4278
BsaBI GATnn'nnATC
Cuts at: 6 351 2658 4695 6
Size: 345 2307 2037 455
Fragments arranged by size:
2307 2037 455 345
BsaHI Gr'CG_yC
Cuts at: 229 2575 2823 3525 5021 229
Size: 2346 248 702 1496 352
Fragments arranged by size:
2346 1496 702 352 248
BsaWI w'CCGG_w
Cuts at: 2333 2854 3532 3806 3953 4364 2333
Size: 521 678 274 147 411 3113
Fragments arranged by size:
3113 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 199
Size: 30 5114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 876 879 1003 484
Size: 392 3 124 4625
Fragments arranged by size:
4625 392 124 3
BseYI C'CCAG_C
Cuts at: 269 434 635 2569 3327 3904 4400 269
Size: 165 201 1934 758 577 496 1013
Fragments arranged by size:
1934 1013 758 577 496 201 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1788 878
Size: 910 4234
BsiEI CG_ry'CG
Cuts at: 104 236 2686 2732 3940 104
Size: 132 2450 46 1208 1308
Fragments arranged by size:
2450 1308 1208 132 46
BsiHKAI G_wGCw'C
Cuts at: 822 995 2158 2936 3126 3918 822
Size: 173 1163 778 190 792 2048
Fragments arranged by size:
2048 1163 792 778 190 173
BsmI GAATG_Cn'
Cuts at: 1644 1644
Size: 5144
BsmBI CGTCTCn'nnnn_
Cuts at: 220 220
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 475 822 995 1445 2024 2051 2158 2769 2862
Size: 347 173 450 579 27 107 611 93
Cuts at: 2862 2936 3126 3188 3918 475
Size: 74 190 62 730 1701
Fragments arranged by size:
1701 730 611 579 450 347 190 173
107 93 74 62 27
BspEI T'CCGG_A
Cuts at: 4364 4364
Size: 5144
BspHI T'CATG_A
Cuts at: 4320 4320
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 615 1134 1220 2194 2408 2710 3091 615
Size: 519 86 974 214 302 381 2668
Fragments arranged by size:
2668 974 519 381 302 214 86
BsrBI CCG'CTC
Cuts at: 50 3436 3490 4974 5139 50
Size: 3386 54 1484 165 55
Fragments arranged by size:
3386 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 3056 1940
Size: 1116 4028
BsrFI r'CCGG_y
Cuts at: 231 3142 3323 3532 231
Size: 2911 181 209 1843
Fragments arranged by size:
2911 1843 209 181
BsrGI T'GTAC_A
Cuts at: 1149 2004 1149
Size: 855 4289
BssSI C'ACGA_G
Cuts at: 3415 3773 3415
Size: 358 4786
BstAPI GCAn_nnn'nTGC
Cuts at: 465 1462 1995 2521 465
Size: 997 533 526 3088
Fragments arranged by size:
3088 997 533 526
BstBI TT'CG_AA
Cuts at: 3505 3505
Size: 5144
BstEII G'GTnAC_C
Cuts at: 1759 1759
Size: 5144
BstYI r'GATC_y
Cuts at: 1 449 864 1577 1978 2169 2636 2659 2994
Size: 448 415 713 401 191 467 23 335
Cuts at: 2994 3240 4252 4338 4350 4696 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 713 467 449 448 415 401 346
335 246 191 86 23 12
BstZ17I GTA'TAC
Cuts at: 1852 1852
Size: 5144
BtgI C'CryG_G
Cuts at: 236 1874 236
Size: 1638 3506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3094 3275 3438 3094
Size: 181 163 4800
Fragments arranged by size:
4800 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1649 2387 1058
Size: 591 738 3815
Fragments arranged by size:
3815 738 591
DraI TTT'AAA
Cuts at: 2143 2143
Size: 5144
DraIII CAC_nnn'GTG
Cuts at: 1401 1401
Size: 5144
DrdI GACnn_nn'nnGTC
Cuts at: 2850 3708 2850
Size: 858 4286
EaeI y'GGCC_r
Cuts at: 233 1036 1625 2729 2903 3294 3321 4666 233
Size: 803 589 1104 174 391 27 1345 711
Fragments arranged by size:
1345 1104 803 711 589 391 174 27
EagI C'GGCC_G
Cuts at: 233 2729 233
Size: 2496 2648
EarI CTCTTCn'nnn_
Cuts at: 389 1913 2012 3167 3377 389
Size: 1524 99 1155 210 2156
Fragments arranged by size:
2156 1524 1155 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3352 3662 3808 4954 3352
Size: 310 146 1146 3542
Fragments arranged by size:
3542 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2156 2156
Size: 5144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 301 370 439 469 481 1535 2063 2969
Size: 24 69 69 30 12 1054 528 906
Cuts at: 2969 3401 4148 4416 277
Size: 432 747 268 1005
Fragments arranged by size:
1054 1005 906 747 528 432 268 69
69 30 24 12
EcoO109I rG'GnC_Cy
Cuts at: 510 1366 1405 2047 2048 2283 510
Size: 856 39 642 1 235 3371
Fragments arranged by size:
3371 856 642 235 39 1
EcoRI G'AATT_C
Cuts at: 1726 2148 1726
Size: 422 4722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 927 3163 927
Size: 2236 2908
FspI TGC'GCA
Cuts at: 1466 2925 1466
Size: 1459 3685
HaeII r_GCGC'y
Cuts at: 711 2578 2826 3848 4827 711
Size: 1867 248 1022 979 1028
Fragments arranged by size:
1867 1028 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 402 434 804 836 1054 1086 2986
Size: 32 171 32 370 32 218 32 1900
Cuts at: 2986 3016 3018 3048 199
Size: 30 2 30 2295
Fragments arranged by size:
2295 1900 370 218 171 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2183 2183
Size: 5144
KasI G'GCGC_C
Cuts at: 2574 2822 2574
Size: 248 4896
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 805 1351 1638 3815 3999 242
Size: 563 546 287 2177 184 1387
Fragments arranged by size:
2177 1387 563 546 287 184
MscI TGG'CCA
Cuts at: 1038 2905 1038
Size: 1867 3277
MslI CAynn'nnrTG
Cuts at: 1214 1390 1597 3133 3260 1214
Size: 176 207 1536 127 3098
Fragments arranged by size:
3098 1536 207 176 127
MspA1I CmG'CkG
Cuts at: 253 2255 2569 2929 3942 4187 253
Size: 2002 314 360 1013 245 1210
Fragments arranged by size:
2002 1210 1013 360 314 245
NaeI GCC'GGC
Cuts at: 233 3325 233
Size: 3092 2052
NarI GG'CG_CC
Cuts at: 2575 2823 2575
Size: 248 4896
NcoI C'CATG_G
Cuts at: 1874 1874
Size: 5144
NdeI CA'TA_TG
Cuts at: 1515 1515
Size: 5144
NgoMIV G'CCGG_C
Cuts at: 231 3323 231
Size: 3092 2052
NsiI A_TGCA'T
Cuts at: 1521 1521
Size: 5144
NspI r_CATG'y
Cuts at: 598 881 931 1102 1219 1395 1785 2197 2697
Size: 283 50 171 117 176 390 412 500
Cuts at: 2697 3604 598
Size: 907 2138
Fragments arranged by size:
2138 907 500 412 390 283 176 171
117 50
PciI A'CATG_T
Cuts at: 877 3600 877
Size: 2723 2421
PflMI CCAn_nnn'nTGG
Cuts at: 642 642
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PmlI CAC'GTG
Cuts at: 1631 1631
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PpuMI rG'GwC_Cy
Cuts at: 1405 1405
Size: 5144
PshAI GACnn'nnGTC
Cuts at: 880 1354 1967 880
Size: 474 613 4057
Fragments arranged by size:
4057 613 474
PsiI TTA'TAA
Cuts at: 548 1484 548
Size: 936 4208
PspOMI G'GGCC_C
Cuts at: 2047 2047
Size: 5144
PstI C_TGCA'G
Cuts at: 794 831 2191 2876 794
Size: 37 1360 685 3062
Fragments arranged by size:
3062 1360 685 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 253 2569 2929 253
Size: 2316 360 2468
Fragments arranged by size:
2468 2316 360
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 2158 2158
Size: 5144
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SanDI GG'GwC_CC
Cuts at: 1405 1405
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 389 1913 2012 3167 3377 389
Size: 1524 99 1155 210 2156
Fragments arranged by size:
2156 1524 1155 210 99
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SfcI C'TryA_G
Cuts at: 33 790 827 1165 1226 2187 2872 3865 4056
Size: 757 37 338 61 961 685 993 191
Cuts at: 4056 33
Size: 1121
Fragments arranged by size:
1121 993 961 757 685 338 191 61
37
SfoI GGC'GCC
Cuts at: 2576 2824 2576
Size: 248 4896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 735 2166 735
Size: 1431 3713
SmlI C'TyrA_G
Cuts at: 419 767 2299 3214 3706 3968 419
Size: 348 1532 915 492 262 1595
Fragments arranged by size:
1595 1532 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5144
SphI G_CATG'C
Cuts at: 1102 1395 2197 2697 1102
Size: 293 802 500 3549
Fragments arranged by size:
3549 802 500 293
SrfI GCCC'GGGC
Cuts at: 735 735
Size: 5144
StuI AGG'CCT
Cuts at: 716 716
Size: 5144
StyI C'CwwG_G
Cuts at: 711 1362 1874 2278 2632 711
Size: 651 512 404 354 3223
Fragments arranged by size:
3223 651 512 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1375 2727 2860 3319 3528 1375
Size: 1352 133 459 209 2991
Fragments arranged by size:
2991 1352 459 209 133
TatI w'GTAC_w
Cuts at: 1117 1134 1149 2004 4659 1117
Size: 17 15 855 2655 1602
Fragments arranged by size:
2655 1602 855 17 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4661 4693 4661
Size: 32 5112
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1012 2639 1012
Size: 1627 3517
XmaI C'CCGG_G
Cuts at: 733 2164 733
Size: 1431 3713
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BglI BmtI BsiWI BssHII BstXI
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PasI PfoI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI