Restriction map for
pF1KB6242
(Circular) (Six-base) MAPSORT of: pF1KB6242.seq Check: 6713 from: 1 to: 5174
pF1KB6242 5174 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1191 1191
Size: 5174
AccI GT'mk_AC
Cuts at: 1159 2065 2212 1159
Size: 906 147 4121
Fragments arranged by size:
4121 906 147
Acc65I G'GTAC_C
Cuts at: 1862 2190 1862
Size: 328 4846
AclI AA'CG_TT
Cuts at: 5014 5014
Size: 5174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 1561 2153 2999 3431 4178 4446 1354
Size: 207 592 846 432 747 268 2082
Fragments arranged by size:
2082 846 747 592 432 268 207
AflIII A'CryG_T
Cuts at: 2526 3630 4607 2526
Size: 1104 977 3093
Fragments arranged by size:
3093 1104 977
AgeI A'CCGG_T
Cuts at: 3562 3562
Size: 5174
AhdI GACnn_n'nnGTC
Cuts at: 2061 2061
Size: 5174
AleI CACnn'nnGTG
Cuts at: 510 510
Size: 5174
AlwNI CAG_nnn'CTG
Cuts at: 567 2479 4046 567
Size: 1912 1567 1695
Fragments arranged by size:
1912 1695 1567
ApaLI G'TGCA_C
Cuts at: 1588 3944 1588
Size: 2356 2818
ApoI r'AATT_y
Cuts at: 282 776 1055 1398 1939 2072 2178 4527 4783
Size: 494 279 343 541 133 106 2349 256
Cuts at: 4783 282
Size: 673
Fragments arranged by size:
2349 673 541 494 343 279 256 133
106
AseI AT'TA_AT
Cuts at: 20 20
Size: 5174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
AvaI C'yCGr_G
Cuts at: 2194 2194
Size: 5174
BamHI G'GATC_C
Cuts at: 123 240 259 2199 123
Size: 117 19 1940 3098
Fragments arranged by size:
3098 1940 117 19
BanI G'GyrC_C
Cuts at: 141 1862 2190 2604 2852 2887 141
Size: 1721 328 414 248 35 2428
Fragments arranged by size:
2428 1721 414 328 248 35
BanII G_rGCy'C
Cuts at: 689 1888 2188 3218 689
Size: 1199 300 1030 2645
Fragments arranged by size:
2645 1199 1030 300
BbeI G_GCGC'C
Cuts at: 2608 2856 2608
Size: 248 4926
BbsI GAAGACnn'nnnn_
Cuts at: 949 1103 1504 2021 949
Size: 154 401 517 4102
Fragments arranged by size:
4102 517 401 154
BciVI GTATCCnnnnn_n'
Cuts at: 892 3072 3833 5174 892
Size: 2180 761 1341 892
Fragments arranged by size:
2180 1341 892 761
BclI T'GATC_A
Cuts at: 1283 2694 1283
Size: 1411 3763
BglII A'GATC_T
Cuts at: 1599 2689 4726 1599
Size: 1090 2037 2047
Fragments arranged by size:
2047 2037 1090
BlpI GC'TnA_GC
Cuts at: 2286 2286
Size: 5174
Bme1580I G_kGCm'C
Cuts at: 1592 2799 2892 3948 1592
Size: 1207 93 1056 2818
Fragments arranged by size:
2818 1207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2386 2467 2802 4946 2386
Size: 81 335 2144 2614
Fragments arranged by size:
2614 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 669 1907 3541 669
Size: 1238 1634 2302
Fragments arranged by size:
2302 1634 1238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1553 1609 604
Size: 949 56 4169
Fragments arranged by size:
4169 949 56
Bpu10I CC'TnA_GC
Cuts at: 422 422
Size: 5174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2350 3229 3721 4019 2036
Size: 314 879 492 298 3191
Fragments arranged by size:
3191 879 492 314 298
BsaI GGTCTCn'nnnn_
Cuts at: 571 610 1673 4878 571
Size: 39 1063 3205 867
Fragments arranged by size:
3205 1063 867 39
BsaAI yAC'GTr
Cuts at: 2527 2527
Size: 5174
BsaBI GATnn'nnATC
Cuts at: 6 1253 2688 4725 6
Size: 1247 1435 2037 455
Fragments arranged by size:
2037 1435 1247 455
BsaHI Gr'CG_yC
Cuts at: 1820 2605 2853 3555 5051 1820
Size: 785 248 702 1496 1943
Fragments arranged by size:
1943 1496 785 702 248
BsaWI w'CCGG_w
Cuts at: 1649 2363 2884 3562 3836 3983 4394 1649
Size: 714 521 678 274 147 411 2429
Fragments arranged by size:
2429 714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 125 155 1183 1213 125
Size: 30 1028 30 4086
Fragments arranged by size:
4086 1028 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 360 565 601 1675 1927 2113 360
Size: 205 36 1074 252 186 3421
Fragments arranged by size:
3421 1074 252 205 186 36
BseYI C'CCAG_C
Cuts at: 2599 3357 3934 4430 2599
Size: 758 577 496 3343
Fragments arranged by size:
3343 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 1880 1616
Size: 264 4910
BsiEI CG_ry'CG
Cuts at: 104 2716 2762 3970 104
Size: 2612 46 1208 1308
Fragments arranged by size:
2612 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 492 689 1592 1873 2188 2966 3156 3948 492
Size: 197 903 281 315 778 190 792 1718
Fragments arranged by size:
1718 903 792 778 315 281 197 190
Bsp1286I G_dGCh'C
Cuts at: 492 689 1592 1873 1888 2188 2799 2892 2966
Size: 197 903 281 15 300 611 93 74
Cuts at: 2966 3156 3218 3948 492
Size: 190 62 730 1718
Fragments arranged by size:
1718 903 730 611 300 281 197 190
93 74 62 15
BspEI T'CCGG_A
Cuts at: 4394 4394
Size: 5174
BspHI T'CATG_A
Cuts at: 4350 4350
Size: 5174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1191 2224 2438 2740 3121 1191
Size: 1033 214 302 381 3244
Fragments arranged by size:
3244 1033 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3466 3520 5004 5169 50
Size: 3416 54 1484 165 55
Fragments arranged by size:
3416 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 374 3086 374
Size: 2712 2462
BsrFI r'CCGG_y
Cuts at: 992 1919 3172 3353 3562 992
Size: 927 1253 181 209 2604
Fragments arranged by size:
2604 1253 927 209 181
BsrGI T'GTAC_A
Cuts at: 1313 1313
Size: 5174
BssSI C'ACGA_G
Cuts at: 3445 3803 3445
Size: 358 4816
BstAPI GCAn_nnn'nTGC
Cuts at: 1231 2551 1231
Size: 1320 3854
BstBI TT'CG_AA
Cuts at: 3535 3535
Size: 5174
BstEII G'GTnAC_C
Cuts at: 988 988
Size: 5174
BstYI r'GATC_y
Cuts at: 1 123 240 259 1599 2199 2666 2689 3024
Size: 122 117 19 1340 600 467 23 335
Cuts at: 3024 3270 4282 4368 4380 4726 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1340 1012 600 467 449 346 335 246
122 117 86 23 19 12
BtgI C'CryG_G
Cuts at: 107 137 1739 107
Size: 30 1602 3542
Fragments arranged by size:
3542 1602 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3124 3305 3468 3124
Size: 181 163 4830
Fragments arranged by size:
4830 181 163
BtsI GCAGTG_nn'
Cuts at: 2034 2417 2034
Size: 383 4791
ClaI AT'CG_AT
Cuts at: 321 321
Size: 5174
DraI TTT'AAA
Cuts at: 666 1053 1362 2173 666
Size: 387 309 811 3667
Fragments arranged by size:
3667 811 387 309
DrdI GACnn_nn'nnGTC
Cuts at: 2880 3738 2880
Size: 858 4316
EaeI y'GGCC_r
Cuts at: 431 569 2759 2933 3324 3351 4696 431
Size: 138 2190 174 391 27 1345 909
Fragments arranged by size:
2190 1345 909 391 174 138 27
EagI C'GGCC_G
Cuts at: 2759 2759
Size: 5174
EarI CTCTTCn'nnn_
Cuts at: 2090 2111 3197 3407 2090
Size: 21 1086 210 3857
Fragments arranged by size:
3857 1086 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 3382 3692 3838 4984 1660
Size: 1722 310 146 1146 1850
Fragments arranged by size:
1850 1722 1146 310 146
EcoICRI GAG'CTC
Cuts at: 687 2186 687
Size: 1499 3675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1354 1553 1561 1609 2153 2999 3431 4178
Size: 750 199 8 48 544 846 432 747
Cuts at: 4178 4446 604
Size: 268 1332
Fragments arranged by size:
1332 846 750 747 544 432 268 199
48 8
EcoNI CCTnn'n_nnAGG
Cuts at: 925 925
Size: 5174
EcoO109I rG'GnC_Cy
Cuts at: 2313 2313
Size: 5174
EcoRI G'AATT_C
Cuts at: 2178 2178
Size: 5174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 937 1439 3193 937
Size: 502 1754 2918
Fragments arranged by size:
2918 1754 502
FspI TGC'GCA
Cuts at: 2955 2955
Size: 5174
HaeII r_GCGC'y
Cuts at: 2608 2856 3878 4857 2608
Size: 248 1022 979 2925
Fragments arranged by size:
2925 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 663 695 724 756 1960 1992 2047
Size: 32 235 32 29 32 1204 32 55
Cuts at: 2047 2079 3016 3046 3048 3078 396
Size: 32 937 30 2 30 2492
Fragments arranged by size:
2492 1204 937 235 55 32 32 32
32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 298 2213 298
Size: 1915 3259
KasI G'GCGC_C
Cuts at: 2604 2852 2604
Size: 248 4926
KpnI G_GTAC'C
Cuts at: 1866 2194 1866
Size: 328 4846
MluI A'CGCG_T
Cuts at: 4607 4607
Size: 5174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 680 811 3845 4029 350
Size: 330 131 3034 184 1495
Fragments arranged by size:
3034 1495 330 184 131
MscI TGG'CCA
Cuts at: 433 571 2935 433
Size: 138 2364 2672
Fragments arranged by size:
2672 2364 138
MslI CAynn'nnrTG
Cuts at: 395 510 1106 1112 1262 3163 3290 395
Size: 115 596 6 150 1901 127 2279
Fragments arranged by size:
2279 1901 596 150 127 115 6
MspA1I CmG'CkG
Cuts at: 1629 2285 2599 2959 3972 4217 1629
Size: 656 314 360 1013 245 2586
Fragments arranged by size:
2586 1013 656 360 314 245
NaeI GCC'GGC
Cuts at: 1921 3355 1921
Size: 1434 3740
NarI GG'CG_CC
Cuts at: 2605 2853 2605
Size: 248 4926
NcoI C'CATG_G
Cuts at: 107 107
Size: 5174
NgoMIV G'CCGG_C
Cuts at: 1919 3353 1919
Size: 1434 3740
NspI r_CATG'y
Cuts at: 2227 2727 3634 2227
Size: 500 907 3767
Fragments arranged by size:
3767 907 500
PciI A'CATG_T
Cuts at: 3630 3630
Size: 5174
PflMI CCAn_nnn'nTGG
Cuts at: 567 892 1347 567
Size: 325 455 4394
Fragments arranged by size:
4394 455 325
PfoI T'CCnGG_A
Cuts at: 361 361
Size: 5174
PmeI GTTT'AAAC
Cuts at: 2173 2173
Size: 5174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 5142
PshAI GACnn'nnGTC
Cuts at: 306 599 822 306
Size: 293 223 4658
Fragments arranged by size:
4658 293 223
PstI C_TGCA'G
Cuts at: 1146 2029 2221 2906 1146
Size: 883 192 685 3414
Fragments arranged by size:
3414 883 685 192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5174
PvuII CAG'CTG
Cuts at: 1629 2599 2959 1629
Size: 970 360 3844
Fragments arranged by size:
3844 970 360
RsrII CG'GwC_CG
Cuts at: 1647 3369 1647
Size: 1722 3452
SacI G_AGCT'C
Cuts at: 689 2188 689
Size: 1499 3675
SalI G'TCGA_C
Cuts at: 2211 2211
Size: 5174
SapI GCTCTTCn'nnn_
Cuts at: 3197 3407 3197
Size: 210 4964
SbfI CC_TGCA'GG
Cuts at: 2221 2221
Size: 5174
ScaI AGT'ACT
Cuts at: 4691 4691
Size: 5174
SfcI C'TryA_G
Cuts at: 33 192 622 1142 2025 2217 2902 3895 4086
Size: 159 430 520 883 192 685 993 191
Cuts at: 4086 33
Size: 1121
Fragments arranged by size:
1121 993 883 685 520 430 192 191
159
SfoI GGC'GCC
Cuts at: 2606 2854 2606
Size: 248 4926
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
SmaI CCC'GGG
Cuts at: 2196 2196
Size: 5174
SmlI C'TyrA_G
Cuts at: 2051 2329 3244 3736 3998 2051
Size: 278 915 492 262 3227
Fragments arranged by size:
3227 915 492 278 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5174
SphI G_CATG'C
Cuts at: 2227 2727 2227
Size: 500 4674
StuI AGG'CCT
Cuts at: 556 556
Size: 5174
StyI C'CwwG_G
Cuts at: 107 236 886 926 1674 2308 2662 107
Size: 129 650 40 748 634 354 2619
Fragments arranged by size:
2619 748 650 634 354 129 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 522 701 706 1697 2757 2890 3349 3558 522
Size: 179 5 991 1060 133 459 209 2138
Fragments arranged by size:
2138 1060 991 459 209 179 133 5
TatI w'GTAC_w
Cuts at: 1313 2047 4689 1313
Size: 734 2642 1798
Fragments arranged by size:
2642 1798 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 710 742 1720 1752 4691 4723 125
Size: 32 553 32 978 32 2939 32 576
Fragments arranged by size:
2939 978 576 553 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 230 1807 2971 230
Size: 1577 1164 2433
Fragments arranged by size:
2433 1577 1164
XbaI T'CTAG_A
Cuts at: 2205 2205
Size: 5174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 2669 579
Size: 2090 3084
XmaI C'CCGG_G
Cuts at: 2194 2194
Size: 5174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstXI BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI