Restriction map for
pF1KB6243
(Circular) (Six-base) MAPSORT of: pF1KB6243.seq Check: 5259 from: 1 to: 5372
pF1KB6243 5372 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2410 2410
Size: 5372
Acc65I G'GTAC_C
Cuts at: 524 2388 524
Size: 1864 3508
AclI AA'CG_TT
Cuts at: 790 5212 790
Size: 4422 950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 2129 2204 3197 3629 4376 4644 827
Size: 1302 75 993 432 747 268 1555
Fragments arranged by size:
1555 1302 993 747 432 268 75
AflIII A'CryG_T
Cuts at: 2724 3828 4805 2724
Size: 1104 977 3291
Fragments arranged by size:
3291 1104 977
AgeI A'CCGG_T
Cuts at: 3760 3760
Size: 5372
AhdI GACnn_n'nnGTC
Cuts at: 2292 2292
Size: 5372
AlwNI CAG_nnn'CTG
Cuts at: 1150 1713 2152 2283 2677 4244 1150
Size: 563 439 131 394 1567 2278
Fragments arranged by size:
2278 1567 563 439 394 131
ApaI G_GGCC'C
Cuts at: 2114 2114
Size: 5372
ApaLI G'TGCA_C
Cuts at: 685 4142 685
Size: 3457 1915
ApoI r'AATT_y
Cuts at: 1164 1236 1344 1871 2376 4725 4981 1164
Size: 72 108 527 505 2349 256 1555
Fragments arranged by size:
2349 1555 527 505 256 108 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 5372
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
AvaI C'yCGr_G
Cuts at: 2158 2392 2158
Size: 234 5138
AvrII C'CTAG_G
Cuts at: 1452 1452
Size: 5372
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 516 549 1901 1934 516
Size: 33 1352 33 3954
Fragments arranged by size:
3954 1352 33 33
BamHI G'GATC_C
Cuts at: 671 2397 671
Size: 1726 3646
BanI G'GyrC_C
Cuts at: 213 524 1578 1723 1840 2388 2802 3050 3085
Size: 311 1054 145 117 548 414 248 35
Cuts at: 3085 213
Size: 2500
Fragments arranged by size:
2500 1054 548 414 311 248 145 117
35
BanII G_rGCy'C
Cuts at: 1691 1945 2095 2114 2386 3416 1691
Size: 254 150 19 272 1030 3647
Fragments arranged by size:
3647 1030 272 254 150 19
BbeI G_GCGC'C
Cuts at: 2806 3054 2806
Size: 248 5124
BbsI GAAGACnn'nnnn_
Cuts at: 598 598
Size: 5372
BbvCI CC'TCA_GC
Cuts at: 1192 1419 1192
Size: 227 5145
BciVI GTATCCnnnnn_n'
Cuts at: 1092 3270 4031 5372 1092
Size: 2178 761 1341 1092
Fragments arranged by size:
2178 1341 1092 761
BclI T'GATC_A
Cuts at: 1976 2892 1976
Size: 916 4456
BglII A'GATC_T
Cuts at: 2887 4924 2887
Size: 2037 3335
BlpI GC'TnA_GC
Cuts at: 2484 2484
Size: 5372
Bme1580I G_kGCm'C
Cuts at: 689 2114 2997 3090 4146 689
Size: 1425 883 93 1056 1915
Fragments arranged by size:
1915 1425 1056 883 93
BmgBI CAC'GTC
Cuts at: 717 717
Size: 5372
BmrI ACTGGGnnnn_n'
Cuts at: 977 2584 2665 3000 5144 977
Size: 1607 81 335 2144 1205
Fragments arranged by size:
2144 1607 1205 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3739 3739
Size: 5372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1493 2066 2260 1493
Size: 573 194 4605
Fragments arranged by size:
4605 573 194
Bpu10I CC'TnA_GC
Cuts at: 187 1192 1419 187
Size: 1005 227 4140
Fragments arranged by size:
4140 1005 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1149 2548 3427 3919 4217 1149
Size: 1399 879 492 298 2304
Fragments arranged by size:
2304 1399 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5076 5076
Size: 5372
BsaAI yAC'GTr
Cuts at: 678 2725 678
Size: 2047 3325
BsaBI GATnn'nnATC
Cuts at: 6 1103 1981 2886 4923 6
Size: 1097 878 905 2037 455
Fragments arranged by size:
2037 1097 905 878 455
BsaHI Gr'CG_yC
Cuts at: 2803 3051 3753 5249 2803
Size: 248 702 1496 2926
Fragments arranged by size:
2926 1496 702 248
BsaWI w'CCGG_w
Cuts at: 495 2561 3082 3760 4034 4181 4592 495
Size: 2066 521 678 274 147 411 1275
Fragments arranged by size:
2066 1275 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1755 1785 1836 1866 1755
Size: 30 51 30 5261
Fragments arranged by size:
5261 51 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 617 1424 2058 2175 2347 2350 617
Size: 807 634 117 172 3 3639
Fragments arranged by size:
3639 807 634 172 117 3
BseYI C'CCAG_C
Cuts at: 1400 1466 1615 2113 2127 2797 3555 4132 4628
Size: 66 149 498 14 670 758 577 496
Cuts at: 4628 1400
Size: 2144
Fragments arranged by size:
2144 758 670 577 498 496 149 66
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 739 409
Size: 330 5042
BsiEI CG_ry'CG
Cuts at: 104 2914 2960 4168 104
Size: 2810 46 1208 1308
Fragments arranged by size:
2810 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 689 1945 2386 3164 3354 4146 689
Size: 1256 441 778 190 792 1915
Fragments arranged by size:
1915 1256 792 778 441 190
BsmI GAATG_Cn'
Cuts at: 736 736
Size: 5372
Bsp1286I G_dGCh'C
Cuts at: 689 1691 1945 2095 2114 2386 2997 3090 3164
Size: 1002 254 150 19 272 611 93 74
Cuts at: 3164 3354 3416 4146 689
Size: 190 62 730 1915
Fragments arranged by size:
1915 1002 730 611 272 254 190 150
93 74 62 19
BspEI T'CCGG_A
Cuts at: 495 4592 495
Size: 4097 1275
BspHI T'CATG_A
Cuts at: 363 1044 4548 363
Size: 681 3504 1187
Fragments arranged by size:
3504 1187 681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 584 1422 2422 2636 2938 3319 584
Size: 838 1000 214 302 381 2637
Fragments arranged by size:
2637 1000 838 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2040 3664 3718 5202 5367 50
Size: 1990 1624 54 1484 165 55
Fragments arranged by size:
1990 1624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 377 3284 377
Size: 2907 2465
BsrFI r'CCGG_y
Cuts at: 3370 3551 3760 3370
Size: 181 209 4982
Fragments arranged by size:
4982 209 181
BsrGI T'GTAC_A
Cuts at: 749 749
Size: 5372
BssSI C'ACGA_G
Cuts at: 3643 4001 3643
Size: 358 5014
BstAPI GCAn_nnn'nTGC
Cuts at: 2749 2749
Size: 5372
BstBI TT'CG_AA
Cuts at: 858 3733 858
Size: 2875 2497
BstXI CCAn_nnnn'nTGG
Cuts at: 1957 1957
Size: 5372
BstYI r'GATC_y
Cuts at: 1 671 1263 2397 2864 2887 3222 3468 4480
Size: 670 592 1134 467 23 335 246 1012
Cuts at: 4480 4566 4578 4924 1
Size: 86 12 346 449
Fragments arranged by size:
1134 1012 670 592 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 667 667
Size: 5372
BtgI C'CryG_G
Cuts at: 107 1700 2249 107
Size: 1593 549 3230
Fragments arranged by size:
3230 1593 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3322 3503 3666 3322
Size: 181 163 5028
Fragments arranged by size:
5028 181 163
BtsI GCAGTG_nn'
Cuts at: 1646 1910 2615 1646
Size: 264 705 4403
Fragments arranged by size:
4403 705 264
DraI TTT'AAA
Cuts at: 2371 2371
Size: 5372
DraIII CAC_nnn'GTG
Cuts at: 838 838
Size: 5372
DrdI GACnn_nn'nnGTC
Cuts at: 3078 3936 3078
Size: 858 4514
EaeI y'GGCC_r
Cuts at: 2957 3131 3522 3549 4894 2957
Size: 174 391 27 1345 3435
Fragments arranged by size:
3435 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2957 2957
Size: 5372
EarI CTCTTCn'nnn_
Cuts at: 335 1367 1988 2148 3395 3605 335
Size: 1032 621 160 1247 210 2102
Fragments arranged by size:
2102 1247 1032 621 210 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1319 1427 3580 3890 4036 5182 1319
Size: 108 2153 310 146 1146 1509
Fragments arranged by size:
2153 1509 1146 310 146 108
EcoICRI GAG'CTC
Cuts at: 1943 2384 1943
Size: 441 4931
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 1493 2066 2129 2204 2260 3197 3629 4376
Size: 666 573 63 75 56 937 432 747
Cuts at: 4376 4644 827
Size: 268 1555
Fragments arranged by size:
1555 937 747 666 573 432 268 75
63 56
EcoO109I rG'GnC_Cy
Cuts at: 267 1188 1456 2511 267
Size: 921 268 1055 3128
Fragments arranged by size:
3128 1055 921 268
EcoRI G'AATT_C
Cuts at: 1236 2376 1236
Size: 1140 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 667 1579 3391 667
Size: 912 1812 2648
Fragments arranged by size:
2648 1812 912
FspI TGC'GCA
Cuts at: 3153 3153
Size: 5372
HaeII r_GCGC'y
Cuts at: 2806 3054 4076 5055 2806
Size: 248 1022 979 3123
Fragments arranged by size:
3123 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1297 1329 2029 2061 2254 2278 2286 2310 3214
Size: 32 700 32 193 24 8 24 904
Cuts at: 3214 3244 3246 3276 1297
Size: 30 2 30 3393
Fragments arranged by size:
3393 904 700 193 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 2411 2411
Size: 5372
KasI G'GCGC_C
Cuts at: 2802 3050 2802
Size: 248 5124
KpnI G_GTAC'C
Cuts at: 528 2392 528
Size: 1864 3508
MluI A'CGCG_T
Cuts at: 4805 4805
Size: 5372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 467 4043 4227 467
Size: 3576 184 1612
Fragments arranged by size:
3576 1612 184
MscI TGG'CCA
Cuts at: 3133 3133
Size: 5372
MslI CAynn'nnrTG
Cuts at: 445 708 885 959 2004 2254 3361 3488 445
Size: 263 177 74 1045 250 1107 127 2329
Fragments arranged by size:
2329 1107 1045 263 250 177 127 74
MspA1I CmG'CkG
Cuts at: 1636 1651 2483 2797 3157 4170 4415 1636
Size: 15 832 314 360 1013 245 2593
Fragments arranged by size:
2593 1013 832 360 314 245 15
NaeI GCC'GGC
Cuts at: 3553 3553
Size: 5372
NarI GG'CG_CC
Cuts at: 2803 3051 2803
Size: 248 5124
NcoI C'CATG_G
Cuts at: 107 1700 2249 107
Size: 1593 549 3230
Fragments arranged by size:
3230 1593 549
NgoMIV G'CCGG_C
Cuts at: 3551 3551
Size: 5372
NspI r_CATG'y
Cuts at: 2003 2021 2425 2925 3832 2003
Size: 18 404 500 907 3543
Fragments arranged by size:
3543 907 500 404 18
PasI CC'CwG_GG
Cuts at: 378 1179 1719 1936 378
Size: 801 540 217 3814
Fragments arranged by size:
3814 801 540 217
PciI A'CATG_T
Cuts at: 3828 3828
Size: 5372
PflMI CCAn_nnn'nTGG
Cuts at: 2283 2283
Size: 5372
PmeI GTTT'AAAC
Cuts at: 2371 2371
Size: 5372
PmlI CAC'GTG
Cuts at: 678 678
Size: 5372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 4511
Size: 32 5340
PpuMI rG'GwC_Cy
Cuts at: 267 1188 1456 267
Size: 921 268 4183
Fragments arranged by size:
4183 921 268
PspOMI G'GGCC_C
Cuts at: 2110 2110
Size: 5372
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1805 1837 1805
Size: 32 5340
PstI C_TGCA'G
Cuts at: 485 817 1793 1917 2123 2327 2419 3104 485
Size: 332 976 124 206 204 92 685 2753
Fragments arranged by size:
2753 976 685 332 206 204 124 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5372
PvuII CAG'CTG
Cuts at: 1651 2797 3157 1651
Size: 1146 360 3866
Fragments arranged by size:
3866 1146 360
RsrII CG'GwC_CG
Cuts at: 3567 3567
Size: 5372
SacI G_AGCT'C
Cuts at: 1945 2386 1945
Size: 441 4931
SalI G'TCGA_C
Cuts at: 2409 2409
Size: 5372
SapI GCTCTTCn'nnn_
Cuts at: 3395 3605 3395
Size: 210 5162
SbfI CC_TGCA'GG
Cuts at: 2419 2419
Size: 5372
ScaI AGT'ACT
Cuts at: 1680 4889 1680
Size: 3209 2163
SfcI C'TryA_G
Cuts at: 33 481 558 813 1789 1913 2119 2323 2415
Size: 448 77 255 976 124 206 204 92
Cuts at: 2415 3100 4093 4284 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 976 685 448 255 206 204
191 124 92 77
SfoI GGC'GCC
Cuts at: 2804 3052 2804
Size: 248 5124
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
SmaI CCC'GGG
Cuts at: 2160 2394 2160
Size: 234 5138
SmlI C'TyrA_G
Cuts at: 1128 2527 3442 3934 4196 1128
Size: 1399 915 492 262 2304
Fragments arranged by size:
2304 1399 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5372
SphI G_CATG'C
Cuts at: 2021 2425 2925 2021
Size: 404 500 4468
Fragments arranged by size:
4468 500 404
SspI AAT'ATT
Cuts at: 459 459
Size: 5372
StyI C'CwwG_G
Cuts at: 107 1452 1700 2249 2363 2506 2860 107
Size: 1345 248 549 114 143 354 2619
Fragments arranged by size:
2619 1345 549 354 248 143 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 459 1711 2955 3088 3547 3756 459
Size: 1252 1244 133 459 209 2075
Fragments arranged by size:
2075 1252 1244 459 209 133
TatI w'GTAC_w
Cuts at: 608 749 1371 1678 4887 608
Size: 141 622 307 3209 1093
Fragments arranged by size:
3209 1093 622 307 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1755 1787 1836 1868 4889 4921 1755
Size: 32 49 32 3021 32 2206
Fragments arranged by size:
3021 2206 49 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3169 3169
Size: 5372
XbaI T'CTAG_A
Cuts at: 2403 2403
Size: 5372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1803 2104 2867 1803
Size: 301 763 4308
Fragments arranged by size:
4308 763 301
XmaI C'CCGG_G
Cuts at: 2158 2392 2158
Size: 234 5138
XmnI GAAnn'nnTTC
Cuts at: 736 1003 1871 736
Size: 267 868 4237
Fragments arranged by size:
4237 868 267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
BcgI BfrBI BglI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PshAI PsiI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI