Restriction map for
pF1KB6248
(Circular) (Six-base) MAPSORT of: pF1KB6248.seq Check: 4583 from: 1 to: 5156
pF1KB6248 5156 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 531 863 1341 531
Size: 332 478 4346
Fragments arranged by size:
4346 478 332
AccI GT'mk_AC
Cuts at: 2194 2194
Size: 5156
Acc65I G'GTAC_C
Cuts at: 2172 2172
Size: 5156
AclI AA'CG_TT
Cuts at: 4996 4996
Size: 5156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3413 4160 4428 2981
Size: 432 747 268 3709
Fragments arranged by size:
3709 747 432 268
AflIII A'CryG_T
Cuts at: 727 2508 3612 4589 727
Size: 1781 1104 977 1294
Fragments arranged by size:
1781 1294 1104 977
AgeI A'CCGG_T
Cuts at: 2043 3544 2043
Size: 1501 3655
AleI CACnn'nnGTG
Cuts at: 169 703 169
Size: 534 4622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 5156
AlwNI CAG_nnn'CTG
Cuts at: 334 472 1734 2461 4028 334
Size: 138 1262 727 1567 1462
Fragments arranged by size:
1567 1462 1262 727 138
ApaLI G'TGCA_C
Cuts at: 946 1114 3926 946
Size: 168 2812 2176
Fragments arranged by size:
2812 2176 168
ApoI r'AATT_y
Cuts at: 152 406 608 2160 4509 4765 152
Size: 254 202 1552 2349 256 543
Fragments arranged by size:
2349 1552 543 256 254 202
AseI AT'TA_AT
Cuts at: 20 20
Size: 5156
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5156
AvaI C'yCGr_G
Cuts at: 951 2176 951
Size: 1225 3931
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1011 1044 1179 1212 1011
Size: 33 135 33 4955
Fragments arranged by size:
4955 135 33 33
BamHI G'GATC_C
Cuts at: 776 2181 776
Size: 1405 3751
BanI G'GyrC_C
Cuts at: 692 1551 1620 2172 2586 2834 2869 692
Size: 859 69 552 414 248 35 2979
Fragments arranged by size:
2979 859 552 414 248 69 35
BanII G_rGCy'C
Cuts at: 145 2170 3200 145
Size: 2025 1030 2101
Fragments arranged by size:
2101 2025 1030
BbeI G_GCGC'C
Cuts at: 2590 2838 2590
Size: 248 4908
BbsI GAAGACnn'nnnn_
Cuts at: 2040 2040
Size: 5156
BciVI GTATCCnnnnn_n'
Cuts at: 1207 3054 3815 5156 1207
Size: 1847 761 1341 1207
Fragments arranged by size:
1847 1341 1207 761
BclI T'GATC_A
Cuts at: 2676 2676
Size: 5156
BglI GCCn_nnn'nGGC
Cuts at: 701 1687 701
Size: 986 4170
BglII A'GATC_T
Cuts at: 809 2671 4708 809
Size: 1862 2037 1257
Fragments arranged by size:
2037 1862 1257
BlpI GC'TnA_GC
Cuts at: 2268 2268
Size: 5156
Bme1580I G_kGCm'C
Cuts at: 950 1118 1556 1577 1625 2781 2874 3930 950
Size: 168 438 21 48 1156 93 1056 2176
Fragments arranged by size:
2176 1156 1056 438 168 93 48 21
BmgBI CAC'GTC
Cuts at: 728 1479 728
Size: 751 4405
BmrI ACTGGGnnnn_n'
Cuts at: 1572 2368 2449 2784 4928 1572
Size: 796 81 335 2144 1800
Fragments arranged by size:
2144 1800 796 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1158 3523 1158
Size: 2365 2791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 819 1149 1474 1531 1543 386
Size: 433 330 325 57 12 3999
Fragments arranged by size:
3999 433 330 325 57 12
Bpu10I CC'TnA_GC
Cuts at: 1264 1264
Size: 5156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 2332 3211 3703 4001 997
Size: 1335 879 492 298 2152
Fragments arranged by size:
2152 1335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 370 4860 370
Size: 4490 666
BsaAI yAC'GTr
Cuts at: 2509 2509
Size: 5156
BsaBI GATnn'nnATC
Cuts at: 6 2670 4707 6
Size: 2664 2037 455
Fragments arranged by size:
2664 2037 455
BsaHI Gr'CG_yC
Cuts at: 875 2587 2835 3537 5033 875
Size: 1712 248 702 1496 998
Fragments arranged by size:
1712 1496 998 702 248
BsaWI w'CCGG_w
Cuts at: 1097 2043 2345 2866 3544 3818 3965 4376 1097
Size: 946 302 521 678 274 147 411 1877
Fragments arranged by size:
1877 946 678 521 411 302 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1147 1177 1147
Size: 30 5126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2043 2046 2043
Size: 3 5153
BseYI C'CCAG_C
Cuts at: 298 1255 1507 1564 1802 2063 2581 3339 3916
Size: 957 252 57 238 261 518 758 577
Cuts at: 3916 4412 298
Size: 496 1042
Fragments arranged by size:
1042 957 758 577 518 496 261 252
238 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 978 1188 1837 978
Size: 210 649 4297
Fragments arranged by size:
4297 649 210
BsiEI CG_ry'CG
Cuts at: 104 1212 2698 2744 3952 104
Size: 1108 1486 46 1208 1308
Fragments arranged by size:
1486 1308 1208 1108 46
BsiHKAI G_wGCw'C
Cuts at: 950 1118 1144 2170 2948 3138 3930 950
Size: 168 26 1026 778 190 792 2176
Fragments arranged by size:
2176 1026 792 778 190 168 26
BsmI GAATG_Cn'
Cuts at: 1153 1153
Size: 5156
Bsp1286I G_dGCh'C
Cuts at: 145 950 1118 1144 1556 1577 1625 2170 2781
Size: 805 168 26 412 21 48 545 611
Cuts at: 2781 2874 2948 3138 3200 3930 145
Size: 93 74 190 62 730 1371
Fragments arranged by size:
1371 805 730 611 545 412 190 168
93 74 62 48 26 21
BspEI T'CCGG_A
Cuts at: 1097 4376 1097
Size: 3279 1877
BspHI T'CATG_A
Cuts at: 4332 4332
Size: 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 446 531 863 1341 2206 2420 2722 3103 446
Size: 85 332 478 865 214 302 381 2499
Fragments arranged by size:
2499 865 478 381 332 302 214 85
BsrBI CCG'CTC
Cuts at: 50 597 792 3448 3502 4986 5151 50
Size: 547 195 2656 54 1484 165 55
Fragments arranged by size:
2656 1484 547 195 165 55 54
BsrDI GCAATG_nn'
Cuts at: 990 1062 1802 3068 990
Size: 72 740 1266 3078
Fragments arranged by size:
3078 1266 740 72
BsrFI r'CCGG_y
Cuts at: 1330 1346 1721 1806 2043 3154 3335 3544 1330
Size: 16 375 85 237 1111 181 209 2942
Fragments arranged by size:
2942 1111 375 237 209 181 85 16
BssSI C'ACGA_G
Cuts at: 1189 1828 3427 3785 1189
Size: 639 1599 358 2560
Fragments arranged by size:
2560 1599 639 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1232 1620 1734 2533 1232
Size: 388 114 799 3855
Fragments arranged by size:
3855 799 388 114
BstBI TT'CG_AA
Cuts at: 3517 3517
Size: 5156
BstYI r'GATC_y
Cuts at: 1 776 809 1730 2181 2648 2671 3006 3252
Size: 775 33 921 451 467 23 335 246
Cuts at: 3252 4264 4350 4362 4708 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 921 775 467 451 449 346 335
246 86 33 23 12
Bsu36I CC'TnA_GG
Cuts at: 772 1878 772
Size: 1106 4050
BtgI C'CryG_G
Cuts at: 443 1287 1871 443
Size: 844 584 3728
Fragments arranged by size:
3728 844 584
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1412 2004 2018 3106 3287 3450 1412
Size: 592 14 1088 181 163 3118
Fragments arranged by size:
3118 1088 592 181 163 14
BtsI GCAGTG_nn'
Cuts at: 1200 1383 1667 2030 2399 1200
Size: 183 284 363 369 3957
Fragments arranged by size:
3957 369 363 284 183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 243 278 243
Size: 35 5121
DraI TTT'AAA
Cuts at: 605 2155 605
Size: 1550 3606
DrdI GACnn_nn'nnGTC
Cuts at: 2862 3720 2862
Size: 858 4298
EaeI y'GGCC_r
Cuts at: 1355 2027 2741 2915 3306 3333 4678 1355
Size: 672 714 174 391 27 1345 1833
Fragments arranged by size:
1833 1345 714 672 391 174 27
EagI C'GGCC_G
Cuts at: 2741 2741
Size: 5156
EarI CTCTTCn'nnn_
Cuts at: 1369 1587 2064 3179 3389 1369
Size: 218 477 1115 210 3136
Fragments arranged by size:
3136 1115 477 218 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3364 3674 3820 4966 3364
Size: 310 146 1146 3554
Fragments arranged by size:
3554 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2168 2168
Size: 5156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 819 1149 1474 1531 1543 2981 3413 4160
Size: 433 330 325 57 12 1438 432 747
Cuts at: 4160 4428 386
Size: 268 1114
Fragments arranged by size:
1438 1114 747 433 432 330 325 268
57 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1738 1738
Size: 5156
EcoO109I rG'GnC_Cy
Cuts at: 2295 2295
Size: 5156
EcoRI G'AATT_C
Cuts at: 2160 2160
Size: 5156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 5156
FspI TGC'GCA
Cuts at: 1183 2937 1183
Size: 1754 3402
HaeII r_GCGC'y
Cuts at: 905 2041 2590 2838 3860 4839 905
Size: 1136 549 248 1022 979 1222
Fragments arranged by size:
1222 1136 1022 979 549 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1296 1328 1586 1618 2998 3028 3030 3060 1296
Size: 32 258 32 1380 30 2 30 3392
Fragments arranged by size:
3392 1380 258 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2195 2195
Size: 5156
KasI G'GCGC_C
Cuts at: 2586 2834 2586
Size: 248 4908
KpnI G_GTAC'C
Cuts at: 2176 2176
Size: 5156
MluI A'CGCG_T
Cuts at: 4589 4589
Size: 5156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1103 3827 4011 1103
Size: 2724 184 2248
Fragments arranged by size:
2724 2248 184
MscI TGG'CCA
Cuts at: 1357 2029 2917 1357
Size: 672 888 3596
Fragments arranged by size:
3596 888 672
MslI CAynn'nnrTG
Cuts at: 169 635 703 943 2023 3145 3272 169
Size: 466 68 240 1080 1122 127 2053
Fragments arranged by size:
2053 1122 1080 466 240 127 68
MspA1I CmG'CkG
Cuts at: 273 742 992 1040 1657 2267 2581 2941 3954
Size: 469 250 48 617 610 314 360 1013
Cuts at: 3954 4199 273
Size: 245 1230
Fragments arranged by size:
1230 1013 617 610 469 360 314 250
245 48
NaeI GCC'GGC
Cuts at: 1348 1723 1808 3337 1348
Size: 375 85 1529 3167
Fragments arranged by size:
3167 1529 375 85
NarI GG'CG_CC
Cuts at: 2587 2835 2587
Size: 248 4908
NcoI C'CATG_G
Cuts at: 1287 1287
Size: 5156
NgoMIV G'CCGG_C
Cuts at: 1346 1721 1806 3335 1346
Size: 375 85 1529 3167
Fragments arranged by size:
3167 1529 375 85
NspI r_CATG'y
Cuts at: 690 942 2209 2709 3616 690
Size: 252 1267 500 907 2230
Fragments arranged by size:
2230 1267 907 500 252
PasI CC'CwG_GG
Cuts at: 1711 1711
Size: 5156
PciI A'CATG_T
Cuts at: 3612 3612
Size: 5156
PmeI GTTT'AAAC
Cuts at: 2155 2155
Size: 5156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 5124
PstI C_TGCA'G
Cuts at: 1237 1662 1730 2203 2888 1237
Size: 425 68 473 685 3505
Fragments arranged by size:
3505 685 473 425 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5156
PvuII CAG'CTG
Cuts at: 273 2581 2941 273
Size: 2308 360 2488
Fragments arranged by size:
2488 2308 360
RsrII CG'GwC_CG
Cuts at: 3351 3351
Size: 5156
SacI G_AGCT'C
Cuts at: 2170 2170
Size: 5156
SalI G'TCGA_C
Cuts at: 2193 2193
Size: 5156
SapI GCTCTTCn'nnn_
Cuts at: 3179 3389 3179
Size: 210 4946
SbfI CC_TGCA'GG
Cuts at: 2203 2203
Size: 5156
ScaI AGT'ACT
Cuts at: 4673 4673
Size: 5156
SfcI C'TryA_G
Cuts at: 33 897 1233 1658 1726 2199 2884 3877 4068
Size: 864 336 425 68 473 685 993 191
Cuts at: 4068 33
Size: 1121
Fragments arranged by size:
1121 993 864 685 473 425 336 191
68
SfoI GGC'GCC
Cuts at: 2588 2836 2588
Size: 248 4908
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5156
SmaI CCC'GGG
Cuts at: 2178 2178
Size: 5156
SmlI C'TyrA_G
Cuts at: 1012 2311 3226 3718 3980 1012
Size: 1299 915 492 262 2188
Fragments arranged by size:
2188 1299 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5156
SphI G_CATG'C
Cuts at: 2209 2709 2209
Size: 500 4656
StuI AGG'CCT
Cuts at: 1955 1955
Size: 5156
StyI C'CwwG_G
Cuts at: 1287 1682 2290 2644 1287
Size: 395 608 354 3799
Fragments arranged by size:
3799 608 395 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 1066 1840 2739 2872 3331 3540 355
Size: 711 774 899 133 459 209 1971
Fragments arranged by size:
1971 899 774 711 459 209 133
TatI w'GTAC_w
Cuts at: 1454 4671 1454
Size: 3217 1939
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 4673 4705 844
Size: 32 3797 32 1295
Fragments arranged by size:
3797 1295 32 32
Tth111I GACn'n_nGTC
Cuts at: 2953 2953
Size: 5156
XbaI T'CTAG_A
Cuts at: 1314 2187 1314
Size: 873 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1897 2651 1897
Size: 754 4402
XmaI C'CCGG_G
Cuts at: 2176 2176
Size: 5156
XmnI GAAnn'nnTTC
Cuts at: 964 1048 1282 1378 964
Size: 84 234 96 4742
Fragments arranged by size:
4742 234 96 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI