Restriction map for
pF1KB6249
(Circular) (Six-base) MAPSORT of: pF1KB6249.seq Check: 9823 from: 1 to: 5381
pF1KB6249 5381 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 716 2419 716
Size: 1703 3678
Acc65I G'GTAC_C
Cuts at: 2397 2397
Size: 5381
AclI AA'CG_TT
Cuts at: 5221 5221
Size: 5381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 1876 2101 2258 3206 3638 4385 4653 1447
Size: 429 225 157 948 432 747 268 2175
Fragments arranged by size:
2175 948 747 432 429 268 225 157
AflIII A'CryG_T
Cuts at: 139 2733 3837 4814 139
Size: 2594 1104 977 706
Fragments arranged by size:
2594 1104 977 706
AgeI A'CCGG_T
Cuts at: 3769 3769
Size: 5381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1462 1462
Size: 5381
AlwNI CAG_nnn'CTG
Cuts at: 672 1164 1425 2686 4253 672
Size: 492 261 1261 1567 1800
Fragments arranged by size:
1800 1567 1261 492 261
ApaI G_GGCC'C
Cuts at: 995 995
Size: 5381
ApaLI G'TGCA_C
Cuts at: 4151 4151
Size: 5381
ApoI r'AATT_y
Cuts at: 1515 2385 4734 4990 1515
Size: 870 2349 256 1906
Fragments arranged by size:
2349 1906 870 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
AvaI C'yCGr_G
Cuts at: 335 1841 2401 335
Size: 1506 560 3315
Fragments arranged by size:
3315 1506 560
AvrII C'CTAG_G
Cuts at: 606 606
Size: 5381
BamHI G'GATC_C
Cuts at: 978 2406 978
Size: 1428 3953
BanI G'GyrC_C
Cuts at: 281 366 466 733 956 1066 2397 2811 3059
Size: 85 100 267 223 110 1331 414 248
Cuts at: 3059 3094 281
Size: 35 2568
Fragments arranged by size:
2568 1331 414 267 248 223 110 100
85 35
BanII G_rGCy'C
Cuts at: 257 995 1358 1676 1759 2069 2395 3425 257
Size: 738 363 318 83 310 326 1030 2213
Fragments arranged by size:
2213 1030 738 363 326 318 310 83
BbeI G_GCGC'C
Cuts at: 370 2815 3063 370
Size: 2445 248 2688
Fragments arranged by size:
2688 2445 248
BbsI GAAGACnn'nnnn_
Cuts at: 854 2035 854
Size: 1181 4200
BbvCI CC'TCA_GC
Cuts at: 1384 1384
Size: 5381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 667 1186 1220 633
Size: 34 519 34 4794
Fragments arranged by size:
4794 519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1354 3279 4040 5381 1354
Size: 1925 761 1341 1354
Fragments arranged by size:
1925 1354 1341 761
BclI T'GATC_A
Cuts at: 2901 2901
Size: 5381
BglI GCCn_nnn'nGGC
Cuts at: 1763 1763
Size: 5381
BglII A'GATC_T
Cuts at: 414 1307 2896 4933 414
Size: 893 1589 2037 862
Fragments arranged by size:
2037 1589 893 862
BlpI GC'TnA_GC
Cuts at: 256 917 2493 256
Size: 661 1576 3144
Fragments arranged by size:
3144 1576 661
Bme1580I G_kGCm'C
Cuts at: 976 995 1069 3006 3099 4155 976
Size: 19 74 1937 93 1056 2202
Fragments arranged by size:
2202 1937 1056 93 74 19
BmrI ACTGGGnnnn_n'
Cuts at: 2593 2674 3009 5153 2593
Size: 81 335 2144 2821
Fragments arranged by size:
2821 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 433 2388 3748 433
Size: 1955 1360 2066
Fragments arranged by size:
2066 1955 1360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 709 744 946 1609 1668 2343 2383 307
Size: 402 35 202 663 59 675 40 3305
Fragments arranged by size:
3305 675 663 402 202 59 40 35
Bpu10I CC'TnA_GC
Cuts at: 1384 2204 1384
Size: 820 4561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 2557 3436 3928 4226 1528
Size: 1029 879 492 298 2683
Fragments arranged by size:
2683 1029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1838 5085 1838
Size: 3247 2134
BsaAI yAC'GTr
Cuts at: 986 2734 986
Size: 1748 3633
BsaBI GATnn'nnATC
Cuts at: 6 232 295 2895 4932 6
Size: 226 63 2600 2037 455
Fragments arranged by size:
2600 2037 455 226 63
BsaHI Gr'CG_yC
Cuts at: 367 2812 3060 3762 5258 367
Size: 2445 248 702 1496 490
Fragments arranged by size:
2445 1496 702 490 248
BsaWI w'CCGG_w
Cuts at: 419 2570 3091 3769 4043 4190 4601 419
Size: 2151 521 678 274 147 411 1199
Fragments arranged by size:
2151 1199 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 425 1165 1471 1767 1813 425
Size: 740 306 296 46 3993
Fragments arranged by size:
3993 740 306 296 46
BseYI C'CCAG_C
Cuts at: 2806 3564 4141 4637 2806
Size: 758 577 496 3550
Fragments arranged by size:
3550 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 2238 814
Size: 1424 3957
BsiEI CG_ry'CG
Cuts at: 104 2923 2969 4177 104
Size: 2819 46 1208 1308
Fragments arranged by size:
2819 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 1676 2069 2354 2395 3173 3363 4155 257
Size: 1419 393 285 41 778 190 792 1483
Fragments arranged by size:
1483 1419 792 778 393 285 190 41
BsmI GAATG_Cn'
Cuts at: 801 2142 801
Size: 1341 4040
Bsp1286I G_dGCh'C
Cuts at: 257 976 995 1069 1358 1676 1759 2069 2354
Size: 719 19 74 289 318 83 310 285
Cuts at: 2354 2395 3006 3099 3173 3363 3425 4155 257
Size: 41 611 93 74 190 62 730 1483
Fragments arranged by size:
1483 730 719 611 318 310 289 285
190 93 83 74 74 62 41 19
BspEI T'CCGG_A
Cuts at: 419 4601 419
Size: 4182 1199
BspHI T'CATG_A
Cuts at: 299 516 4557 299
Size: 217 4041 1123
Fragments arranged by size:
4041 1123 217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 2431 2645 2947 3328 386
Size: 2045 214 302 381 2439
Fragments arranged by size:
2439 2045 381 302 214
BsrBI CCG'CTC
Cuts at: 50 276 3673 3727 5211 5376 50
Size: 226 3397 54 1484 165 55
Fragments arranged by size:
3397 1484 226 165 55 54
BsrDI GCAATG_nn'
Cuts at: 491 3293 491
Size: 2802 2579
BsrFI r'CCGG_y
Cuts at: 245 1442 3379 3560 3769 245
Size: 1197 1937 181 209 1857
Fragments arranged by size:
1937 1857 1197 209 181
BsrGI T'GTAC_A
Cuts at: 1817 1817
Size: 5381
BssSI C'ACGA_G
Cuts at: 3652 4010 3652
Size: 358 5023
BstAPI GCAn_nnn'nTGC
Cuts at: 641 792 945 2758 641
Size: 151 153 1813 3264
Fragments arranged by size:
3264 1813 153 151
BstBI TT'CG_AA
Cuts at: 3742 3742
Size: 5381
BstEII G'GTnAC_C
Cuts at: 116 116
Size: 5381
BstXI CCAn_nnnn'nTGG
Cuts at: 327 624 327
Size: 297 5084
BstYI r'GATC_y
Cuts at: 1 290 414 540 978 1260 1307 2406 2873
Size: 289 124 126 438 282 47 1099 467
Cuts at: 2873 2896 3231 3477 4489 4575 4587 4933 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1099 1012 467 449 438 346 335 289
282 246 126 124 86 47 23 12
Bsu36I CC'TnA_GG
Cuts at: 347 347
Size: 5381
BtgI C'CryG_G
Cuts at: 392 1315 1520 1760 2103 2183 392
Size: 923 205 240 343 80 3590
Fragments arranged by size:
3590 923 343 240 205 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3331 3512 3675 3331
Size: 181 163 5037
Fragments arranged by size:
5037 181 163
BtsI GCAGTG_nn'
Cuts at: 247 802 2624 247
Size: 555 1822 3004
Fragments arranged by size:
3004 1822 555
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2085 2120 2085
Size: 35 5346
DraI TTT'AAA
Cuts at: 2380 2380
Size: 5381
DraIII CAC_nnn'GTG
Cuts at: 1214 1214
Size: 5381
DrdI GACnn_nn'nnGTC
Cuts at: 3087 3945 3087
Size: 858 4523
EaeI y'GGCC_r
Cuts at: 389 2966 3140 3531 3558 4903 389
Size: 2577 174 391 27 1345 867
Fragments arranged by size:
2577 1345 867 391 174 27
EagI C'GGCC_G
Cuts at: 2966 2966
Size: 5381
EarI CTCTTCn'nnn_
Cuts at: 814 1991 2212 2342 3404 3614 814
Size: 1177 221 130 1062 210 2581
Fragments arranged by size:
2581 1177 1062 221 210 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3589 3899 4045 5191 3589
Size: 310 146 1146 3779
Fragments arranged by size:
3779 1146 310 146
EcoICRI GAG'CTC
Cuts at: 255 1674 2067 2393 255
Size: 1419 393 326 3243
Fragments arranged by size:
3243 1419 393 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 709 744 946 1447 1609 1668 1876 2101
Size: 402 35 202 501 162 59 208 225
Cuts at: 2101 2258 2343 2383 3206 3638 4385 4653 307
Size: 157 85 40 823 432 747 268 1035
Fragments arranged by size:
1035 823 747 501 432 402 268 225
208 202 162 157 85 59 40 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2365 2365
Size: 5381
EcoO109I rG'GnC_Cy
Cuts at: 146 659 1011 2520 146
Size: 513 352 1509 3007
Fragments arranged by size:
3007 1509 513 352
EcoRI G'AATT_C
Cuts at: 1515 2385 1515
Size: 870 4511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 184 3400 184
Size: 3216 2165
FspI TGC'GCA
Cuts at: 3162 3162
Size: 5381
HaeII r_GCGC'y
Cuts at: 168 370 1975 2815 3063 4085 5064 168
Size: 202 1605 840 248 1022 979 485
Fragments arranged by size:
1605 1022 979 840 485 248 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 1707 1739 3223 3253 3255 3285 481
Size: 32 1194 32 1484 30 2 30 2577
Fragments arranged by size:
2577 1484 1194 32 32 30 30 2
HincII GTy'rAC
Cuts at: 717 2420 717
Size: 1703 3678
KasI G'GCGC_C
Cuts at: 366 2811 3059 366
Size: 2445 248 2688
Fragments arranged by size:
2688 2445 248
KpnI G_GTAC'C
Cuts at: 2401 2401
Size: 5381
MluI A'CGCG_T
Cuts at: 4814 4814
Size: 5381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1152 1535 2216 4052 4236 1152
Size: 383 681 1836 184 2297
Fragments arranged by size:
2297 1836 681 383 184
MscI TGG'CCA
Cuts at: 391 3142 391
Size: 2751 2630
MslI CAynn'nnrTG
Cuts at: 320 3370 3497 320
Size: 3050 127 2204
Fragments arranged by size:
3050 2204 127
MspA1I CmG'CkG
Cuts at: 1340 1383 1496 2166 2357 2492 2806 3166 4179
Size: 43 113 670 191 135 314 360 1013
Cuts at: 4179 4424 1340
Size: 245 2297
Fragments arranged by size:
2297 1013 670 360 314 245 191 135
113 43
NaeI GCC'GGC
Cuts at: 247 3562 247
Size: 3315 2066
NarI GG'CG_CC
Cuts at: 367 2812 3060 367
Size: 2445 248 2688
Fragments arranged by size:
2688 2445 248
NcoI C'CATG_G
Cuts at: 392 1520 1760 392
Size: 1128 240 4013
Fragments arranged by size:
4013 1128 240
NgoMIV G'CCGG_C
Cuts at: 245 3560 245
Size: 3315 2066
NspI r_CATG'y
Cuts at: 143 2434 2934 3841 143
Size: 2291 500 907 1683
Fragments arranged by size:
2291 1683 907 500
PciI A'CATG_T
Cuts at: 139 3837 139
Size: 3698 1683
PflMI CCAn_nnn'nTGG
Cuts at: 113 1251 113
Size: 1138 4243
PmeI GTTT'AAAC
Cuts at: 2380 2380
Size: 5381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 4520
Size: 32 5349
PpuMI rG'GwC_Cy
Cuts at: 659 659
Size: 5381
PshAI GACnn'nnGTC
Cuts at: 2035 2035
Size: 5381
PspOMI G'GGCC_C
Cuts at: 991 991
Size: 5381
PstI C_TGCA'G
Cuts at: 569 668 1109 1172 1451 1730 2048 2428 3113
Size: 99 441 63 279 279 318 380 685
Cuts at: 3113 569
Size: 2837
Fragments arranged by size:
2837 685 441 380 318 279 279 99
63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5381
PvuII CAG'CTG
Cuts at: 1340 1383 1496 2166 2357 2806 3166 1340
Size: 43 113 670 191 449 360 3555
Fragments arranged by size:
3555 670 449 360 191 113 43
RsrII CG'GwC_CG
Cuts at: 3576 3576
Size: 5381
SacI G_AGCT'C
Cuts at: 257 1676 2069 2395 257
Size: 1419 393 326 3243
Fragments arranged by size:
3243 1419 393 326
SalI G'TCGA_C
Cuts at: 715 2418 715
Size: 1703 3678
SanDI GG'GwC_CC
Cuts at: 659 659
Size: 5381
SapI GCTCTTCn'nnn_
Cuts at: 2342 3404 3614 2342
Size: 1062 210 4109
Fragments arranged by size:
4109 1062 210
SbfI CC_TGCA'GG
Cuts at: 2428 2428
Size: 5381
ScaI AGT'ACT
Cuts at: 400 4898 400
Size: 4498 883
SfcI C'TryA_G
Cuts at: 33 565 664 1105 1168 1447 1726 2044 2424
Size: 532 99 441 63 279 279 318 380
Cuts at: 2424 3109 4102 4293 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 532 441 380 318 279
279 191 99 63
SfoI GGC'GCC
Cuts at: 368 2813 3061 368
Size: 2445 248 2688
Fragments arranged by size:
2688 2445 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
SmaI CCC'GGG
Cuts at: 2403 2403
Size: 5381
SmlI C'TyrA_G
Cuts at: 1543 1841 2536 3451 3943 4205 1543
Size: 298 695 915 492 262 2719
Fragments arranged by size:
2719 915 695 492 298 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5381
SphI G_CATG'C
Cuts at: 2434 2934 2434
Size: 500 4881
StuI AGG'CCT
Cuts at: 2371 2371
Size: 5381
StyI C'CwwG_G
Cuts at: 168 392 405 606 617 1520 1760 1988 2515
Size: 224 13 201 11 903 240 228 527
Cuts at: 2515 2869 168
Size: 354 2680
Fragments arranged by size:
2680 903 527 354 240 228 224 201
13 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1747 2964 3097 3556 3765 1747
Size: 1217 133 459 209 3363
Fragments arranged by size:
3363 1217 459 209 133
TatI w'GTAC_w
Cuts at: 398 701 1595 1817 4896 398
Size: 303 894 222 3079 883
Fragments arranged by size:
3079 894 883 303 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4898 4930 4898
Size: 32 5349
Tth111I GACn'n_nGTC
Cuts at: 3178 3178
Size: 5381
XbaI T'CTAG_A
Cuts at: 2412 2412
Size: 5381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2876 2876
Size: 5381
XhoI C'TCGA_G
Cuts at: 1841 1841
Size: 5381
XmaI C'CCGG_G
Cuts at: 2401 2401
Size: 5381
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BssHII
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI