Restriction map for
pF1KB6250
(Circular) (Six-base) MAPSORT of: pF1KB6250.seq Check: 3079 from: 1 to: 5159
pF1KB6250 5159 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2197 2197
Size: 5159
Acc65I G'GTAC_C
Cuts at: 392 2175 392
Size: 1783 3376
AclI AA'CG_TT
Cuts at: 4999 4999
Size: 5159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 945 1029 1216 1365 1449 1533 1617 1636
Size: 732 84 187 149 84 84 84 19
Cuts at: 1636 1701 1869 1953 2037 2984 3416 4163 4431
Size: 65 168 84 84 947 432 747 268
Cuts at: 4431 213
Size: 941
Fragments arranged by size:
947 941 747 732 432 268 187 168
149 84 84 84 84 84 84 65
19
AfeI AGC'GCT
Cuts at: 1882 1882
Size: 5159
AflIII A'CryG_T
Cuts at: 1534 2511 3615 4592 1534
Size: 977 1104 977 2101
Fragments arranged by size:
2101 1104 977 977
AgeI A'CCGG_T
Cuts at: 3547 3547
Size: 5159
AleI CACnn'nnGTG
Cuts at: 584 584
Size: 5159
AlwNI CAG_nnn'CTG
Cuts at: 2464 4031 2464
Size: 1567 3592
ApaLI G'TGCA_C
Cuts at: 3929 3929
Size: 5159
ApoI r'AATT_y
Cuts at: 2163 4512 4768 2163
Size: 2349 256 2554
Fragments arranged by size:
2554 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5159
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5159
AvaI C'yCGr_G
Cuts at: 2150 2179 2150
Size: 29 5130
BamHI G'GATC_C
Cuts at: 2184 2184
Size: 5159
BanI G'GyrC_C
Cuts at: 392 1751 2175 2589 2837 2872 392
Size: 1359 424 414 248 35 2679
Fragments arranged by size:
2679 1359 424 414 248 35
BanII G_rGCy'C
Cuts at: 637 663 1561 1981 2065 2173 3203 637
Size: 26 898 420 84 108 1030 2593
Fragments arranged by size:
2593 1030 898 420 108 84 26
BbeI G_GCGC'C
Cuts at: 2593 2841 2593
Size: 248 4911
BbsI GAAGACnn'nnnn_
Cuts at: 147 718 1959 147
Size: 571 1241 3347
Fragments arranged by size:
3347 1241 571
BciVI GTATCCnnnnn_n'
Cuts at: 3057 3818 5159 3057
Size: 761 1341 3057
Fragments arranged by size:
3057 1341 761
BclI T'GATC_A
Cuts at: 2679 2679
Size: 5159
BfrBI ATG'CAT
Cuts at: 1114 1366 1114
Size: 252 4907
BglII A'GATC_T
Cuts at: 425 1563 2674 4711 425
Size: 1138 1111 2037 873
Fragments arranged by size:
2037 1138 1111 873
BlpI GC'TnA_GC
Cuts at: 2271 2271
Size: 5159
Bme1580I G_kGCm'C
Cuts at: 2784 2877 3933 2784
Size: 93 1056 4010
Fragments arranged by size:
4010 1056 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 5159
BmrI ACTGGGnnnn_n'
Cuts at: 2371 2452 2787 4931 2371
Size: 81 335 2144 2599
Fragments arranged by size:
2599 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 651 857 1277 1361 1529 1629 1697 1781
Size: 410 206 420 84 168 100 68 84
Cuts at: 1781 1995 241
Size: 214 3405
Fragments arranged by size:
3405 420 410 214 206 168 100 84
84 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2049 2335 3214 3706 4004 2049
Size: 286 879 492 298 3204
Fragments arranged by size:
3204 879 492 298 286
BsaI GGTCTCn'nnnn_
Cuts at: 246 1929 4863 246
Size: 1683 2934 542
Fragments arranged by size:
2934 1683 542
BsaAI yAC'GTr
Cuts at: 2512 2512
Size: 5159
BsaBI GATnn'nnATC
Cuts at: 6 2673 4710 6
Size: 2667 2037 455
Fragments arranged by size:
2667 2037 455
BsaHI Gr'CG_yC
Cuts at: 2590 2838 3540 5036 2590
Size: 248 702 1496 2713
Fragments arranged by size:
2713 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2011 2348 2869 3547 3821 3968 4379 2011
Size: 337 521 678 274 147 411 2791
Fragments arranged by size:
2791 678 521 411 337 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 780 810 2052 2082 780
Size: 30 1242 30 3857
Fragments arranged by size:
3857 1242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 181 327 893 958 1210 1294 1462 1798
Size: 3 146 566 65 252 84 168 336
Cuts at: 1798 2050 178
Size: 252 3287
Fragments arranged by size:
3287 566 336 252 252 168 146 84
65 3
BseYI C'CCAG_C
Cuts at: 2584 3342 3919 4415 2584
Size: 758 577 496 3328
Fragments arranged by size:
3328 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1718 1718
Size: 5159
BsiEI CG_ry'CG
Cuts at: 104 2701 2747 3955 104
Size: 2597 46 1208 1308
Fragments arranged by size:
2597 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 637 1561 1981 2065 2173 2951 3141 3933 637
Size: 924 420 84 108 778 190 792 1863
Fragments arranged by size:
1863 924 792 778 420 190 108 84
Bsp1286I G_dGCh'C
Cuts at: 637 663 1561 1981 2065 2173 2784 2877 2951
Size: 26 898 420 84 108 611 93 74
Cuts at: 2951 3141 3203 3933 637
Size: 190 62 730 1863
Fragments arranged by size:
1863 898 730 611 420 190 108 93
84 74 62 26
BspEI T'CCGG_A
Cuts at: 4379 4379
Size: 5159
BspHI T'CATG_A
Cuts at: 402 1071 4335 402
Size: 669 3264 1226
Fragments arranged by size:
3264 1226 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 2209 2423 2725 3106 404
Size: 1805 214 302 381 2457
Fragments arranged by size:
2457 1805 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3451 3505 4989 5154 50
Size: 3401 54 1484 165 55
Fragments arranged by size:
3401 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1116 3071 1116
Size: 1955 3204
BsrFI r'CCGG_y
Cuts at: 3157 3338 3547 3157
Size: 181 209 4769
Fragments arranged by size:
4769 209 181
BsrGI T'GTAC_A
Cuts at: 1572 1572
Size: 5159
BssSI C'ACGA_G
Cuts at: 3430 3788 3430
Size: 358 4801
BstAPI GCAn_nnn'nTGC
Cuts at: 2536 2536
Size: 5159
BstBI TT'CG_AA
Cuts at: 3520 3520
Size: 5159
BstXI CCAn_nnnn'nTGG
Cuts at: 1821 1821
Size: 5159
BstYI r'GATC_y
Cuts at: 1 425 1563 1894 2184 2651 2674 3009 3255
Size: 424 1138 331 290 467 23 335 246
Cuts at: 3255 4267 4353 4365 4711 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1138 1012 467 449 424 346 335 331
290 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 339 339
Size: 5159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3109 3290 3453 3109
Size: 181 163 4815
Fragments arranged by size:
4815 181 163
BtsI GCAGTG_nn'
Cuts at: 134 2402 134
Size: 2268 2891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1098 1133 1098
Size: 35 5124
DraI TTT'AAA
Cuts at: 2158 2158
Size: 5159
DraIII CAC_nnn'GTG
Cuts at: 1831 1831
Size: 5159
DrdI GACnn_nn'nnGTC
Cuts at: 2865 3723 2865
Size: 858 4301
EaeI y'GGCC_r
Cuts at: 2744 2918 3309 3336 4681 2744
Size: 174 391 27 1345 3222
Fragments arranged by size:
3222 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2744 2744
Size: 5159
EarI CTCTTCn'nnn_
Cuts at: 377 401 854 1022 1033 3182 3392 377
Size: 24 453 168 11 2149 210 2144
Fragments arranged by size:
2149 2144 453 210 168 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3367 3677 3823 4969 3367
Size: 310 146 1146 3557
Fragments arranged by size:
3557 1146 310 146
EcoICRI GAG'CTC
Cuts at: 635 1559 1979 2063 2171 635
Size: 924 420 84 108 3623
Fragments arranged by size:
3623 924 420 108 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 241 651 857 945 1029 1216 1277 1361
Size: 28 410 206 88 84 187 61 84
Cuts at: 1361 1365 1449 1529 1533 1617 1629 1636 1697
Size: 4 84 80 4 84 12 7 61
Cuts at: 1697 1701 1781 1869 1953 1995 2037 2984 3416
Size: 4 80 88 84 42 42 947 432
Cuts at: 3416 4163 4431 213
Size: 747 268 941
Fragments arranged by size:
947 941 747 432 410 268 206 187
88 88 84 84 84 84 84 80
80 61 61 42 42 28 12 7
4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2298 2298
Size: 5159
EcoRI G'AATT_C
Cuts at: 2163 2163
Size: 5159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3178 3178
Size: 5159
FspI TGC'GCA
Cuts at: 2940 2940
Size: 5159
HaeII r_GCGC'y
Cuts at: 1884 2593 2841 3863 4842 1884
Size: 709 248 1022 979 2201
Fragments arranged by size:
2201 1022 979 709 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 386 437 469 543 575 624 656
Size: 32 32 51 32 74 32 49 32
Cuts at: 656 780 792 812 824 960 992 1548 1580
Size: 124 12 20 12 136 32 556 32
Cuts at: 1580 2052 2084 3001 3031 3033 3063 322
Size: 472 32 917 30 2 30 2418
Fragments arranged by size:
2418 917 556 472 136 124 74 51
49 32 32 32 32 32 32 32
32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 2198 2198
Size: 5159
KasI G'GCGC_C
Cuts at: 2589 2837 2589
Size: 248 4911
KpnI G_GTAC'C
Cuts at: 396 2179 396
Size: 1783 3376
MluI A'CGCG_T
Cuts at: 4592 4592
Size: 5159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 1534 2038 3830 4014 482
Size: 1052 504 1792 184 1627
Fragments arranged by size:
1792 1627 1052 504 184
MscI TGG'CCA
Cuts at: 2920 2920
Size: 5159
MslI CAynn'nnrTG
Cuts at: 584 1104 1272 1356 1692 1860 1944 2028 2096
Size: 520 168 84 336 168 84 84 68
Cuts at: 2096 3148 3275 584
Size: 1052 127 2468
Fragments arranged by size:
2468 1052 520 336 168 168 127 84
84 84 68
MspA1I CmG'CkG
Cuts at: 629 1973 2270 2584 2944 3957 4202 629
Size: 1344 297 314 360 1013 245 1586
Fragments arranged by size:
1586 1344 1013 360 314 297 245
NaeI GCC'GGC
Cuts at: 3340 3340
Size: 5159
NarI GG'CG_CC
Cuts at: 2590 2838 2590
Size: 248 4911
NgoMIV G'CCGG_C
Cuts at: 3338 3338
Size: 5159
NsiI A_TGCA'T
Cuts at: 1116 1368 1116
Size: 252 4907
NspI r_CATG'y
Cuts at: 1118 1370 1538 2212 2712 3619 1118
Size: 252 168 674 500 907 2658
Fragments arranged by size:
2658 907 674 500 252 168
PciI A'CATG_T
Cuts at: 1534 3615 1534
Size: 2081 3078
PmeI GTTT'AAAC
Cuts at: 2158 2158
Size: 5159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 4298
Size: 32 5127
PspXI vC'TCGA_Gb
Cuts at: 2150 2150
Size: 5159
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 537 569 537
Size: 32 5127
PstI C_TGCA'G
Cuts at: 1067 2206 2891 1067
Size: 1139 685 3335
Fragments arranged by size:
3335 1139 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5159
PvuII CAG'CTG
Cuts at: 629 1973 2584 2944 629
Size: 1344 611 360 2844
Fragments arranged by size:
2844 1344 611 360
RsrII CG'GwC_CG
Cuts at: 3354 3354
Size: 5159
SacI G_AGCT'C
Cuts at: 637 1561 1981 2065 2173 637
Size: 924 420 84 108 3623
Fragments arranged by size:
3623 924 420 108 84
SalI G'TCGA_C
Cuts at: 2196 2196
Size: 5159
SapI GCTCTTCn'nnn_
Cuts at: 401 1033 3182 3392 401
Size: 632 2149 210 2168
Fragments arranged by size:
2168 2149 632 210
SbfI CC_TGCA'GG
Cuts at: 2206 2206
Size: 5159
ScaI AGT'ACT
Cuts at: 4676 4676
Size: 5159
SfcI C'TryA_G
Cuts at: 33 1063 1578 2202 2887 3880 4071 33
Size: 1030 515 624 685 993 191 1121
Fragments arranged by size:
1121 1030 993 685 624 515 191
SfoI GGC'GCC
Cuts at: 2591 2839 2591
Size: 248 4911
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5159
SmaI CCC'GGG
Cuts at: 2181 2181
Size: 5159
SmlI C'TyrA_G
Cuts at: 2064 2150 2314 3229 3721 3983 2064
Size: 86 164 915 492 262 3240
Fragments arranged by size:
3240 915 492 262 164 86
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5159
SphI G_CATG'C
Cuts at: 2212 2712 2212
Size: 500 4659
SspI AAT'ATT
Cuts at: 480 480
Size: 5159
StuI AGG'CCT
Cuts at: 1474 1630 1474
Size: 156 5003
StyI C'CwwG_G
Cuts at: 2293 2647 2293
Size: 354 4805
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2742 2875 3334 3543 2742
Size: 133 459 209 4358
Fragments arranged by size:
4358 459 209 133
TatI w'GTAC_w
Cuts at: 1572 4674 1572
Size: 3102 2057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4676 4708 4676
Size: 32 5127
Tth111I GACn'n_nGTC
Cuts at: 114 2956 114
Size: 2842 2317
XbaI T'CTAG_A
Cuts at: 2190 2190
Size: 5159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2654 2654
Size: 5159
XhoI C'TCGA_G
Cuts at: 2150 2150
Size: 5159
XmaI C'CCGG_G
Cuts at: 2179 2179
Size: 5159
XmnI GAAnn'nnTTC
Cuts at: 622 1042 1210 1294 1546 1714 1798 1966 2050
Size: 420 168 84 252 168 84 168 84
Cuts at: 2050 622
Size: 3731
Fragments arranged by size:
3731 420 252 168 168 168 84 84
84
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstZ17I BtgI ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI ZraI