Restriction map for
pF1KB6254
(Circular) (Six-base) MAPSORT of: pF1KB6254.seq Check: 6752 from: 1 to: 5162
pF1KB6254 5162 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 800 843 1593 800
Size: 43 750 4369
Fragments arranged by size:
4369 750 43
AccI GT'mk_AC
Cuts at: 2200 2200
Size: 5162
Acc65I G'GTAC_C
Cuts at: 1697 2178 1697
Size: 481 4681
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 2987 3419 4166 4434 871
Size: 2116 432 747 268 1599
Fragments arranged by size:
2116 1599 747 432 268
AflIII A'CryG_T
Cuts at: 2514 3618 4595 2514
Size: 1104 977 3081
Fragments arranged by size:
3081 1104 977
AgeI A'CCGG_T
Cuts at: 341 3550 341
Size: 3209 1953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 838 838
Size: 5162
AlwNI CAG_nnn'CTG
Cuts at: 953 1507 1585 1678 2467 4034 953
Size: 554 78 93 789 1567 2081
Fragments arranged by size:
2081 1567 789 554 93 78
ApaI G_GGCC'C
Cuts at: 219 1121 1389 219
Size: 902 268 3992
Fragments arranged by size:
3992 902 268
ApaLI G'TGCA_C
Cuts at: 3932 3932
Size: 5162
ApoI r'AATT_y
Cuts at: 1066 2166 4515 4771 1066
Size: 1100 2349 256 1457
Fragments arranged by size:
2349 1457 1100 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 175 281 349 358 939 1103 1112 1192 1389
Size: 106 68 9 581 164 9 80 197
Cuts at: 1389 2003 2182 175
Size: 614 179 3155
Fragments arranged by size:
3155 614 581 197 179 164 106 80
68 9 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 978 1011 978
Size: 33 5129
BamHI G'GATC_C
Cuts at: 2187 2187
Size: 5162
BanI G'GyrC_C
Cuts at: 354 574 788 1272 1316 1697 1953 2178 2592
Size: 220 214 484 44 381 256 225 414
Cuts at: 2592 2840 2875 354
Size: 248 35 2641
Fragments arranged by size:
2641 484 414 381 256 248 225 220
214 44 35
BanII G_rGCy'C
Cuts at: 219 558 620 1112 1121 1389 1421 1527 1556
Size: 339 62 492 9 268 32 106 29
Cuts at: 1556 2176 3206 219
Size: 620 1030 2175
Fragments arranged by size:
2175 1030 620 492 339 268 106 62
32 29 9
BbeI G_GCGC'C
Cuts at: 1320 2596 2844 1320
Size: 1276 248 3638
Fragments arranged by size:
3638 1276 248
BbsI GAAGACnn'nnnn_
Cuts at: 2091 2091
Size: 5162
BbvCI CC'TCA_GC
Cuts at: 469 469
Size: 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 153 187 153
Size: 34 5128
BciVI GTATCCnnnnn_n'
Cuts at: 3060 3821 5162 3060
Size: 761 1341 3060
Fragments arranged by size:
3060 1341 761
BclI T'GATC_A
Cuts at: 2682 2682
Size: 5162
BglI GCCn_nnn'nGGC
Cuts at: 112 124 1116 1903 2067 112
Size: 12 992 787 164 3207
Fragments arranged by size:
3207 992 787 164 12
BglII A'GATC_T
Cuts at: 2677 4714 2677
Size: 2037 3125
BlpI GC'TnA_GC
Cuts at: 1143 2274 1143
Size: 1131 4031
Bme1580I G_kGCm'C
Cuts at: 176 219 359 1121 1275 1389 1856 1871 1899
Size: 43 140 762 154 114 467 15 28
Cuts at: 1899 1999 2787 2880 3936 176
Size: 100 788 93 1056 1402
Fragments arranged by size:
1402 1056 788 762 467 154 140 114
100 93 43 28 15
BmgBI CAC'GTC
Cuts at: 603 603
Size: 5162
BmrI ACTGGGnnnn_n'
Cuts at: 760 2374 2455 2790 4934 760
Size: 1614 81 335 2144 988
Fragments arranged by size:
2144 1614 988 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1158 3529 1158
Size: 2371 2791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 406 1975 2044 167
Size: 239 1569 69 3285
Fragments arranged by size:
3285 1569 239 69
Bpu10I CC'TnA_GC
Cuts at: 394 469 1420 1656 394
Size: 75 951 236 3900
Fragments arranged by size:
3900 951 236 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1771 1885 2338 3217 3709 4007 1442
Size: 329 114 453 879 492 298 2597
Fragments arranged by size:
2597 879 492 453 329 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 185 1148 1608 1680 4866 185
Size: 963 460 72 3186 481
Fragments arranged by size:
3186 963 481 460 72
BsaAI yAC'GTr
Cuts at: 2106 2515 2106
Size: 409 4753
BsaBI GATnn'nnATC
Cuts at: 6 2676 4713 6
Size: 2670 2037 455
Fragments arranged by size:
2670 2037 455
BsaHI Gr'CG_yC
Cuts at: 1317 2593 2841 3543 5039 1317
Size: 1276 248 702 1496 1440
Fragments arranged by size:
1496 1440 1276 702 248
BsaWI w'CCGG_w
Cuts at: 341 674 1085 2351 2872 3550 3824 3971 4382
Size: 333 411 1266 521 678 274 147 411
Cuts at: 4382 341
Size: 1121
Fragments arranged by size:
1266 1121 678 521 411 411 333 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 189 219 739 769 1145 1175 189
Size: 30 520 30 376 30 4176
Fragments arranged by size:
4176 520 376 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 238 1237 1306 1321 1632 1978 163
Size: 75 999 69 15 311 346 3347
Fragments arranged by size:
3347 999 346 311 75 69 15
BseYI C'CCAG_C
Cuts at: 429 549 708 2587 3345 3922 4418 429
Size: 120 159 1879 758 577 496 1173
Fragments arranged by size:
1879 1173 758 577 496 159 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1183 227
Size: 956 4206
BsiEI CG_ry'CG
Cuts at: 104 119 1843 2704 2750 3958 104
Size: 15 1724 861 46 1208 1308
Fragments arranged by size:
1724 1308 1208 861 46 15
BsiHKAI G_wGCw'C
Cuts at: 620 1975 2176 2954 3144 3936 620
Size: 1355 201 778 190 792 1846
Fragments arranged by size:
1846 1355 792 778 201 190
BsmBI CGTCTCn'nnnn_
Cuts at: 192 759 192
Size: 567 4595
Bsp1286I G_dGCh'C
Cuts at: 176 219 359 558 620 1112 1121 1275 1389
Size: 43 140 199 62 492 9 154 114
Cuts at: 1389 1421 1527 1556 1856 1871 1899 1975 1999
Size: 32 106 29 300 15 28 76 24
Cuts at: 1999 2176 2787 2880 2954 3144 3206 3936 176
Size: 177 611 93 74 190 62 730 1402
Fragments arranged by size:
1402 730 611 492 300 199 190 177
154 140 114 106 93 76 74 62
62 43 32 29 28 24 15 9
BspEI T'CCGG_A
Cuts at: 674 1085 4382 674
Size: 411 3297 1454
Fragments arranged by size:
3297 1454 411
BspHI T'CATG_A
Cuts at: 4338 4338
Size: 5162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 786 800 843 1593 2212 2426 2728 3109 786
Size: 14 43 750 619 214 302 381 2839
Fragments arranged by size:
2839 750 619 381 302 214 43 14
BsrBI CCG'CTC
Cuts at: 50 213 1311 1347 1373 3454 3508 4992 5157
Size: 163 1098 36 26 2081 54 1484 165
Cuts at: 5157 50
Size: 55
Fragments arranged by size:
2081 1484 1098 165 163 55 54 36
26
BsrDI GCAATG_nn'
Cuts at: 3074 3074
Size: 5162
BsrFI r'CCGG_y
Cuts at: 260 341 1296 3160 3341 3550 260
Size: 81 955 1864 181 209 1872
Fragments arranged by size:
1872 1864 955 209 181 81
BssSI C'ACGA_G
Cuts at: 3433 3791 3433
Size: 358 4804
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 2539 1507
Size: 1032 4130
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstYI r'GATC_y
Cuts at: 1 966 1088 1983 2187 2654 2677 3012 3258
Size: 965 122 895 204 467 23 335 246
Cuts at: 3258 4270 4356 4368 4714 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 965 895 467 449 346 335 246
204 122 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 520 784 1778 1958 520
Size: 264 994 180 3724
Fragments arranged by size:
3724 994 264 180
BtgI C'CryG_G
Cuts at: 107 230 1591 107
Size: 123 1361 3678
Fragments arranged by size:
3678 1361 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3112 3293 3456 3112
Size: 181 163 4818
Fragments arranged by size:
4818 181 163
BtsI GCAGTG_nn'
Cuts at: 210 1997 2405 210
Size: 1787 408 2967
Fragments arranged by size:
2967 1787 408
DraI TTT'AAA
Cuts at: 2161 2161
Size: 5162
DrdI GACnn_nn'nnGTC
Cuts at: 670 1186 2868 3726 670
Size: 516 1682 858 2106
Fragments arranged by size:
2106 1682 858 516
EaeI y'GGCC_r
Cuts at: 116 233 1594 1787 1799 1826 1840 2747 2921
Size: 117 1361 193 12 27 14 907 174
Cuts at: 2921 3312 3339 4684 116
Size: 391 27 1345 594
Fragments arranged by size:
1361 1345 907 594 391 193 174 117
27 27 14 12
EagI C'GGCC_G
Cuts at: 116 1840 2747 116
Size: 1724 907 2531
Fragments arranged by size:
2531 1724 907
EarI CTCTTCn'nnn_
Cuts at: 657 698 1199 1636 3185 3395 657
Size: 41 501 437 1549 210 2424
Fragments arranged by size:
2424 1549 501 437 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 718 3370 3680 3826 4972 718
Size: 2652 310 146 1146 908
Fragments arranged by size:
2652 1146 908 310 146
EcoICRI GAG'CTC
Cuts at: 618 2174 618
Size: 1556 3606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 406 871 1975 2044 2987 3419 4166 4434
Size: 239 465 1104 69 943 432 747 268
Cuts at: 4434 167
Size: 895
Fragments arranged by size:
1104 943 895 747 465 432 268 239
69
EcoNI CCTnn'n_nnAGG
Cuts at: 223 1253 1777 1789 223
Size: 1030 524 12 3596
Fragments arranged by size:
3596 1030 524 12
EcoO109I rG'GnC_Cy
Cuts at: 216 242 390 525 663 714 918 1117 1385
Size: 26 148 135 138 51 204 199 268
Cuts at: 1385 1386 1660 1746 1782 2052 2301 216
Size: 1 274 86 36 270 249 3077
Fragments arranged by size:
3077 274 270 268 249 204 199 148
138 135 86 51 36 26 1
EcoRI G'AATT_C
Cuts at: 2166 2166
Size: 5162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 3181 1573
Size: 1608 3554
FspI TGC'GCA
Cuts at: 2943 2943
Size: 5162
HaeII r_GCGC'y
Cuts at: 267 1320 2596 2844 3866 4845 267
Size: 1053 1276 248 1022 979 584
Fragments arranged by size:
1276 1053 1022 979 584 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 607 639 933 965 1957 1989 3004 3034 3036
Size: 32 294 32 992 32 1015 30 2
Cuts at: 3036 3066 607
Size: 30 2703
Fragments arranged by size:
2703 1015 992 294 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1058 2201 1058
Size: 1143 4019
KasI G'GCGC_C
Cuts at: 1316 2592 2840 1316
Size: 1276 248 3638
Fragments arranged by size:
3638 1276 248
KpnI G_GTAC'C
Cuts at: 1701 2182 1701
Size: 481 4681
MfeI C'AATT_G
Cuts at: 408 408
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3833 4017 3833
Size: 184 4978
MscI TGG'CCA
Cuts at: 235 1801 1828 2923 235
Size: 1566 27 1095 2474
Fragments arranged by size:
2474 1566 1095 27
MslI CAynn'nnrTG
Cuts at: 1864 3151 3278 1864
Size: 1287 127 3748
Fragments arranged by size:
3748 1287 127
MspA1I CmG'CkG
Cuts at: 375 483 513 1147 1335 1513 1737 1767 1824
Size: 108 30 634 188 178 224 30 57
Cuts at: 1824 2273 2587 2947 3960 4205 375
Size: 449 314 360 1013 245 1332
Fragments arranged by size:
1332 1013 634 449 360 314 245 224
188 178 108 57 30 30
NaeI GCC'GGC
Cuts at: 1298 3343 1298
Size: 2045 3117
NarI GG'CG_CC
Cuts at: 1317 2593 2841 1317
Size: 1276 248 3638
Fragments arranged by size:
3638 1276 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5162
NgoMIV G'CCGG_C
Cuts at: 1296 3341 1296
Size: 2045 3117
NspI r_CATG'y
Cuts at: 2215 2715 3622 2215
Size: 500 907 3755
Fragments arranged by size:
3755 907 500
PasI CC'CwG_GG
Cuts at: 579 912 1249 579
Size: 333 337 4492
Fragments arranged by size:
4492 337 333
PciI A'CATG_T
Cuts at: 3618 3618
Size: 5162
PfoI T'CCnGG_A
Cuts at: 334 975 334
Size: 641 4521
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 4301
Size: 32 5130
PpuMI rG'GwC_Cy
Cuts at: 242 525 663 714 918 1746 1782 242
Size: 283 138 51 204 828 36 3622
Fragments arranged by size:
3622 828 283 204 138 51 36
PspOMI G'GGCC_C
Cuts at: 215 1117 1385 215
Size: 902 268 3992
Fragments arranged by size:
3992 902 268
PstI C_TGCA'G
Cuts at: 797 827 963 1512 1766 2209 2894 797
Size: 30 136 549 254 443 685 3065
Fragments arranged by size:
3065 685 549 443 254 136 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5162
PvuII CAG'CTG
Cuts at: 375 483 513 1335 1737 1824 2587 2947 375
Size: 108 30 822 402 87 763 360 2590
Fragments arranged by size:
2590 822 763 402 360 108 87 30
RsrII CG'GwC_CG
Cuts at: 2015 3357 2015
Size: 1342 3820
SacI G_AGCT'C
Cuts at: 620 2176 620
Size: 1556 3606
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SanDI GG'GwC_CC
Cuts at: 242 242
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 698 1199 3185 3395 698
Size: 501 1986 210 2465
Fragments arranged by size:
2465 1986 501 210
SbfI CC_TGCA'GG
Cuts at: 797 2209 797
Size: 1412 3750
ScaI AGT'ACT
Cuts at: 4679 4679
Size: 5162
SfcI C'TryA_G
Cuts at: 33 793 823 959 1508 1762 2205 2890 3883
Size: 760 30 136 549 254 443 685 993
Cuts at: 3883 4074 33
Size: 191 1121
Fragments arranged by size:
1121 993 760 685 549 443 254 191
136 30
SfoI GGC'GCC
Cuts at: 1318 2594 2842 1318
Size: 1276 248 3638
Fragments arranged by size:
3638 1276 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 177 283 1105 1114 1194 2005 2184 177
Size: 106 822 9 80 811 179 3155
Fragments arranged by size:
3155 822 811 179 106 80 9
SmlI C'TyrA_G
Cuts at: 1457 1750 1900 2317 3232 3724 3986 1457
Size: 293 150 417 915 492 262 2633
Fragments arranged by size:
2633 915 492 417 293 262 150
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5162
SphI G_CATG'C
Cuts at: 2215 2715 2215
Size: 500 4662
StyI C'CwwG_G
Cuts at: 107 434 2296 2650 107
Size: 327 1862 354 2619
Fragments arranged by size:
2619 1862 354 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 2745 2878 3337 3546 334
Size: 2411 133 459 209 1950
Fragments arranged by size:
2411 1950 459 209 133
TatI w'GTAC_w
Cuts at: 4677 4677
Size: 5162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 189 221 1145 1177 4679 4711 189
Size: 32 924 32 3502 32 640
Fragments arranged by size:
3502 924 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1678 2959 1678
Size: 1281 3881
XbaI T'CTAG_A
Cuts at: 1929 2193 1929
Size: 264 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2657 2657
Size: 5162
XmaI C'CCGG_G
Cuts at: 175 281 1103 1112 1192 2003 2182 175
Size: 106 822 9 80 811 179 3155
Fragments arranged by size:
3155 822 811 179 106 80 9
XmnI GAAnn'nnTTC
Cuts at: 274 274
Size: 5162
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI