Restriction map for
pF1KB6256
(Circular) (Six-base) MAPSORT of: pF1KB6256.seq Check: 197 from: 1 to: 5162
pF1KB6256 5162 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 1339 1339
Size: 5162
AccI GT'mk_AC
Cuts at: 2200 2200
Size: 5162
Acc65I G'GTAC_C
Cuts at: 2178 2178
Size: 5162
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1051 1439 2089 2987 3419 4166 4434 179
Size: 872 388 650 898 432 747 268 907
Fragments arranged by size:
907 898 872 747 650 432 388 268
AflIII A'CryG_T
Cuts at: 2514 3618 4595 2514
Size: 1104 977 3081
Fragments arranged by size:
3081 1104 977
AgeI A'CCGG_T
Cuts at: 3550 3550
Size: 5162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1387 1387
Size: 5162
AlwNI CAG_nnn'CTG
Cuts at: 550 741 1006 1260 1869 2467 4034 550
Size: 191 265 254 609 598 1567 1678
Fragments arranged by size:
1678 1567 609 598 265 254 191
ApaI G_GGCC'C
Cuts at: 1627 1627
Size: 5162
ApaLI G'TGCA_C
Cuts at: 3932 3932
Size: 5162
ApoI r'AATT_y
Cuts at: 1540 2166 4515 4771 1540
Size: 626 2349 256 1931
Fragments arranged by size:
2349 1931 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 2182 2182
Size: 5162
BamHI G'GATC_C
Cuts at: 2187 2187
Size: 5162
BanI G'GyrC_C
Cuts at: 714 816 1565 2178 2592 2840 2875 714
Size: 102 749 613 414 248 35 3001
Fragments arranged by size:
3001 749 613 414 248 102 35
BanII G_rGCy'C
Cuts at: 218 892 1024 1627 2129 2176 3206 218
Size: 674 132 603 502 47 1030 2174
Fragments arranged by size:
2174 1030 674 603 502 132 47
BbeI G_GCGC'C
Cuts at: 718 820 1569 2596 2844 718
Size: 102 749 1027 248 3036
Fragments arranged by size:
3036 1027 749 248 102
BbsI GAAGACnn'nnnn_
Cuts at: 1057 1057
Size: 5162
BbvCI CC'TCA_GC
Cuts at: 821 1832 1838 821
Size: 1011 6 4145
Fragments arranged by size:
4145 1011 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 252 286 252
Size: 34 5128
BciVI GTATCCnnnnn_n'
Cuts at: 1763 3060 3821 5162 1763
Size: 1297 761 1341 1763
Fragments arranged by size:
1763 1341 1297 761
BclI T'GATC_A
Cuts at: 1268 2682 1268
Size: 1414 3748
BglI GCCn_nnn'nGGC
Cuts at: 712 712
Size: 5162
BglII A'GATC_T
Cuts at: 2677 4714 2677
Size: 2037 3125
BlpI GC'TnA_GC
Cuts at: 2274 2274
Size: 5162
Bme1580I G_kGCm'C
Cuts at: 1627 1797 2787 2880 3936 1627
Size: 170 990 93 1056 2853
Fragments arranged by size:
2853 1056 990 170 93
BmrI ACTGGGnnnn_n'
Cuts at: 2374 2455 2790 4934 2374
Size: 81 335 2144 2602
Fragments arranged by size:
2602 2144 335 81
BmtI G_CTAG'C
Cuts at: 1718 1718
Size: 5162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3529 3529
Size: 5162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 613 619 625 876 905 1044 1546 604
Size: 9 6 6 251 29 139 502 4220
Fragments arranged by size:
4220 502 251 139 29 9 6 6
Bpu10I CC'TnA_GC
Cuts at: 821 1832 1838 821
Size: 1011 6 4145
Fragments arranged by size:
4145 1011 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1837 1960 2338 3217 3709 4007 1837
Size: 123 378 879 492 298 2992
Fragments arranged by size:
2992 879 492 378 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 4866 4866
Size: 5162
BsaAI yAC'GTr
Cuts at: 2515 2515
Size: 5162
BsaBI GATnn'nnATC
Cuts at: 6 2676 4713 6
Size: 2670 2037 455
Fragments arranged by size:
2670 2037 455
BsaHI Gr'CG_yC
Cuts at: 715 817 1336 1566 2593 2841 3543 5039 715
Size: 102 519 230 1027 248 702 1496 838
Fragments arranged by size:
1496 1027 838 702 519 248 230 102
BsaWI w'CCGG_w
Cuts at: 2351 2872 3550 3824 3971 4382 2351
Size: 521 678 274 147 411 3131
Fragments arranged by size:
3131 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 1312 1342 835
Size: 30 447 30 4655
Fragments arranged by size:
4655 447 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1198 1428 1437 1543 1198
Size: 230 9 106 4817
Fragments arranged by size:
4817 230 106 9
BseYI C'CCAG_C
Cuts at: 259 288 426 614 728 758 827 881 1202
Size: 29 138 188 114 30 69 54 321
Cuts at: 1202 2587 3345 3922 4418 259
Size: 1385 758 577 496 1003
Fragments arranged by size:
1385 1003 758 577 496 321 188 138
114 69 54 30 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1357 1207
Size: 150 5012
BsiEI CG_ry'CG
Cuts at: 104 2704 2750 3958 104
Size: 2600 46 1208 1308
Fragments arranged by size:
2600 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 892 1024 2176 2954 3144 3936 892
Size: 132 1152 778 190 792 2118
Fragments arranged by size:
2118 1152 792 778 190 132
BsmI GAATG_Cn'
Cuts at: 165 165
Size: 5162
Bsp1286I G_dGCh'C
Cuts at: 218 892 1024 1627 1797 2129 2176 2787 2880
Size: 674 132 603 170 332 47 611 93
Cuts at: 2880 2954 3144 3206 3936 218
Size: 74 190 62 730 1444
Fragments arranged by size:
1444 730 674 611 603 332 190 170
132 93 74 62 47
BspEI T'CCGG_A
Cuts at: 4382 4382
Size: 5162
BspHI T'CATG_A
Cuts at: 4338 4338
Size: 5162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1846 2212 2426 2728 3109 1846
Size: 366 214 302 381 3899
Fragments arranged by size:
3899 381 366 302 214
BsrBI CCG'CTC
Cuts at: 50 945 1821 3454 3508 4992 5157 50
Size: 895 876 1633 54 1484 165 55
Fragments arranged by size:
1633 1484 895 876 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1056 3074 1056
Size: 2018 3144
BsrFI r'CCGG_y
Cuts at: 403 1809 2103 3160 3341 3550 403
Size: 1406 294 1057 181 209 2015
Fragments arranged by size:
2015 1406 1057 294 209 181
BssSI C'ACGA_G
Cuts at: 2098 3433 3791 2098
Size: 1335 358 3469
Fragments arranged by size:
3469 1335 358
BstAPI GCAn_nnn'nTGC
Cuts at: 550 741 1006 2539 550
Size: 191 265 1533 3173
Fragments arranged by size:
3173 1533 265 191
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstYI r'GATC_y
Cuts at: 1 1256 1532 1943 2187 2654 2677 3012 3258
Size: 1255 276 411 244 467 23 335 246
Cuts at: 3258 4270 4356 4368 4714 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1255 1012 467 449 411 346 335 276
246 244 86 23 12
BtgI C'CryG_G
Cuts at: 2108 2108
Size: 5162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1218 3112 3293 3456 1218
Size: 1894 181 163 2924
Fragments arranged by size:
2924 1894 181 163
BtsI GCAGTG_nn'
Cuts at: 502 577 2405 502
Size: 75 1828 3259
Fragments arranged by size:
3259 1828 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1227 1262 1227
Size: 35 5127
DraI TTT'AAA
Cuts at: 1557 2161 1557
Size: 604 4558
DrdI GACnn_nn'nnGTC
Cuts at: 2868 3726 2868
Size: 858 4304
EaeI y'GGCC_r
Cuts at: 401 1308 1643 2105 2747 2921 3312 3339 4684
Size: 907 335 462 642 174 391 27 1345
Cuts at: 4684 401
Size: 879
Fragments arranged by size:
1345 907 879 642 462 391 335 174
27
EagI C'GGCC_G
Cuts at: 2747 2747
Size: 5162
EarI CTCTTCn'nnn_
Cuts at: 274 1434 1455 3185 3395 274
Size: 1160 21 1730 210 2041
Fragments arranged by size:
2041 1730 1160 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3370 3680 3826 4972 3370
Size: 310 146 1146 3560
Fragments arranged by size:
3560 1146 310 146
EcoICRI GAG'CTC
Cuts at: 890 1022 2174 890
Size: 132 1152 3878
Fragments arranged by size:
3878 1152 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 604 613 619 625 876 905 1044 1051
Size: 425 9 6 6 251 29 139 7
Cuts at: 1051 1439 1546 2089 2987 3419 4166 4434 179
Size: 388 107 543 898 432 747 268 907
Fragments arranged by size:
907 898 747 543 432 425 388 268
251 139 107 29 9 7 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1139 1687 1139
Size: 548 4614
EcoO109I rG'GnC_Cy
Cuts at: 421 1623 1624 1935 2301 421
Size: 1202 1 311 366 3282
Fragments arranged by size:
3282 1202 366 311 1
EcoRI G'AATT_C
Cuts at: 2166 2166
Size: 5162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1384 3181 1384
Size: 1797 3365
FspI TGC'GCA
Cuts at: 2943 2943
Size: 5162
HaeII r_GCGC'y
Cuts at: 718 820 1569 2596 2844 3866 4845 718
Size: 102 749 1027 248 1022 979 1035
Fragments arranged by size:
1035 1027 1022 979 749 248 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 482 514 1097 1122 1129 1154 1575 1592 1607
Size: 32 583 25 7 25 421 17 15
Cuts at: 1607 1624 1646 1678 3004 3034 3036 3066 482
Size: 17 22 32 1326 30 2 30 2578
Fragments arranged by size:
2578 1326 583 421 32 32 30 30
25 25 22 17 17 15 7 2
HincII GTy'rAC
Cuts at: 683 1084 2201 683
Size: 401 1117 3644
Fragments arranged by size:
3644 1117 401
HindIII A'AGCT_T
Cuts at: 1550 1550
Size: 5162
KasI G'GCGC_C
Cuts at: 714 816 1565 2592 2840 714
Size: 102 749 1027 248 3036
Fragments arranged by size:
3036 1027 749 248 102
KpnI G_GTAC'C
Cuts at: 2182 2182
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 901 3833 4017 901
Size: 2932 184 2046
Fragments arranged by size:
2932 2046 184
MscI TGG'CCA
Cuts at: 2923 2923
Size: 5162
MslI CAynn'nnrTG
Cuts at: 3151 3278 3151
Size: 127 5035
MspA1I CmG'CkG
Cuts at: 550 741 807 969 1003 1501 2273 2587 2947
Size: 191 66 162 34 498 772 314 360
Cuts at: 2947 3960 4205 550
Size: 1013 245 1507
Fragments arranged by size:
1507 1013 772 498 360 314 245 191
162 66 34
NaeI GCC'GGC
Cuts at: 405 2105 3343 405
Size: 1700 1238 2224
Fragments arranged by size:
2224 1700 1238
NarI GG'CG_CC
Cuts at: 715 817 1566 2593 2841 715
Size: 102 749 1027 248 3036
Fragments arranged by size:
3036 1027 749 248 102
NgoMIV G'CCGG_C
Cuts at: 403 2103 3341 403
Size: 1700 1238 2224
Fragments arranged by size:
2224 1700 1238
NheI G'CTAG_C
Cuts at: 1714 1714
Size: 5162
NspI r_CATG'y
Cuts at: 2215 2715 3622 2215
Size: 500 907 3755
Fragments arranged by size:
3755 907 500
PasI CC'CwG_GG
Cuts at: 1221 1278 1221
Size: 57 5105
PciI A'CATG_T
Cuts at: 3618 3618
Size: 5162
PflMI CCAn_nnn'nTGG
Cuts at: 537 928 537
Size: 391 4771
PfoI T'CCnGG_A
Cuts at: 921 921
Size: 5162
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 4301
Size: 32 5130
PspOMI G'GGCC_C
Cuts at: 1623 1623
Size: 5162
PstI C_TGCA'G
Cuts at: 555 746 1002 1011 1654 2209 2894 555
Size: 191 256 9 643 555 685 2823
Fragments arranged by size:
2823 685 643 555 256 191 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5162
PvuII CAG'CTG
Cuts at: 550 807 969 2587 2947 550
Size: 257 162 1618 360 2765
Fragments arranged by size:
2765 1618 360 257 162
RsrII CG'GwC_CG
Cuts at: 3357 3357
Size: 5162
SacI G_AGCT'C
Cuts at: 892 1024 2176 892
Size: 132 1152 3878
Fragments arranged by size:
3878 1152 132
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 274 3185 3395 274
Size: 2911 210 2041
Fragments arranged by size:
2911 2041 210
SbfI CC_TGCA'GG
Cuts at: 2209 2209
Size: 5162
ScaI AGT'ACT
Cuts at: 4679 4679
Size: 5162
SfcI C'TryA_G
Cuts at: 33 551 742 964 998 1007 1616 1650 1709
Size: 518 191 222 34 9 609 34 59
Cuts at: 1709 2205 2890 3883 4074 33
Size: 496 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 518 496 222 191
191 59 34 34 9
SfoI GGC'GCC
Cuts at: 716 818 1567 2594 2842 716
Size: 102 749 1027 248 3036
Fragments arranged by size:
3036 1027 749 248 102
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 2184 2184
Size: 5162
SmlI C'TyrA_G
Cuts at: 1816 1939 2317 3232 3724 3986 1816
Size: 123 378 915 492 262 2992
Fragments arranged by size:
2992 915 492 378 262 123
SpeI A'CTAG_T
Cuts at: 67 1086 67
Size: 1019 4143
SphI G_CATG'C
Cuts at: 2215 2715 2215
Size: 500 4662
StuI AGG'CCT
Cuts at: 1042 1042
Size: 5162
StyI C'CwwG_G
Cuts at: 531 1359 1575 2296 2650 531
Size: 828 216 721 354 3043
Fragments arranged by size:
3043 828 721 354 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 969 1291 1311 1888 2011 2039 2745 2878 3337
Size: 322 20 577 123 28 706 133 459
Cuts at: 3337 3546 969
Size: 209 2585
Fragments arranged by size:
2585 706 577 459 322 209 133 123
28 20
TatI w'GTAC_w
Cuts at: 4677 4677
Size: 5162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 835 867 1312 1344 4679 4711 835
Size: 32 445 32 3335 32 1286
Fragments arranged by size:
3335 1286 445 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2959 2959
Size: 5162
XbaI T'CTAG_A
Cuts at: 2193 2193
Size: 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2657 2657
Size: 5162
XmaI C'CCGG_G
Cuts at: 2182 2182
Size: 5162
ZraI GAC'GTC
Cuts at: 1337 1337
Size: 5162
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI