Restriction map for
pF1KB6264
(Circular) (Six-base) MAPSORT of: pF1KB6264.seq Check: 2001 from: 1 to: 5420
pF1KB6264 5420 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 302 302
Size: 5420
AccI GT'mk_AC
Cuts at: 798 2458 798
Size: 1660 3760
Acc65I G'GTAC_C
Cuts at: 2436 2436
Size: 5420
AclI AA'CG_TT
Cuts at: 5260 5260
Size: 5420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 341 696 1030 1095 3245 3677 4424 4692
Size: 164 355 334 65 2150 432 747 268
Cuts at: 4692 177
Size: 905
Fragments arranged by size:
2150 905 747 432 355 334 268 164
65
AflIII A'CryG_T
Cuts at: 1059 2261 2772 3876 4853 1059
Size: 1202 511 1104 977 1626
Fragments arranged by size:
1626 1202 1104 977 511
AgeI A'CCGG_T
Cuts at: 1538 3808 1538
Size: 2270 3150
AleI CACnn'nnGTG
Cuts at: 1072 1769 1072
Size: 697 4723
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 299
Size: 32 5388
AlwNI CAG_nnn'CTG
Cuts at: 1013 1265 1937 1971 2189 2725 4292 1013
Size: 252 672 34 218 536 1567 2141
Fragments arranged by size:
2141 1567 672 536 252 218 34
ApaLI G'TGCA_C
Cuts at: 4190 4190
Size: 5420
ApoI r'AATT_y
Cuts at: 508 622 725 809 977 1229 1313 1481 1733
Size: 114 103 84 168 252 84 168 252
Cuts at: 1733 1817 1901 2153 2237 2424 4773 5029 508
Size: 84 84 252 84 187 2349 256 899
Fragments arranged by size:
2349 899 256 252 252 252 187 168
168 114 103 84 84 84 84 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 5420
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
AvaI C'yCGr_G
Cuts at: 2440 2440
Size: 5420
BamHI G'GATC_C
Cuts at: 2445 2445
Size: 5420
BanI G'GyrC_C
Cuts at: 233 2436 2850 3098 3133 233
Size: 2203 414 248 35 2520
Fragments arranged by size:
2520 2203 414 248 35
BanII G_rGCy'C
Cuts at: 2434 3464 2434
Size: 1030 4390
BbeI G_GCGC'C
Cuts at: 2854 3102 2854
Size: 248 5172
BbsI GAAGACnn'nnnn_
Cuts at: 958 1101 2119 958
Size: 143 1018 4259
Fragments arranged by size:
4259 1018 143
BbvCI CC'TCA_GC
Cuts at: 141 141
Size: 5420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5386
BciVI GTATCCnnnnn_n'
Cuts at: 465 488 3318 4079 5420 465
Size: 23 2830 761 1341 465
Fragments arranged by size:
2830 1341 761 465 23
BclI T'GATC_A
Cuts at: 2940 2940
Size: 5420
BfrBI ATG'CAT
Cuts at: 587 885 1343 587
Size: 298 458 4664
Fragments arranged by size:
4664 458 298
BglII A'GATC_T
Cuts at: 789 2935 4972 789
Size: 2146 2037 1237
Fragments arranged by size:
2146 2037 1237
BlpI GC'TnA_GC
Cuts at: 2532 2532
Size: 5420
Bme1580I G_kGCm'C
Cuts at: 366 3045 3138 4194 366
Size: 2679 93 1056 1592
Fragments arranged by size:
2679 1592 1056 93
BmgBI CAC'GTC
Cuts at: 385 385
Size: 5420
BmrI ACTGGGnnnn_n'
Cuts at: 1667 2632 2713 3048 5192 1667
Size: 965 81 335 2144 1895
Fragments arranged by size:
2144 1895 965 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 592 760 3787 592
Size: 168 3027 2225
Fragments arranged by size:
3027 2225 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 298 839 1007 1259 1427 1763 1931 2183
Size: 161 541 168 252 168 336 168 252
Cuts at: 2183 2267 2351 137
Size: 84 84 3206
Fragments arranged by size:
3206 541 336 252 252 168 168 168
161 84 84
Bpu10I CC'TnA_GC
Cuts at: 141 141
Size: 5420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 2596 3475 3967 4265 862
Size: 1734 879 492 298 2017
Fragments arranged by size:
2017 1734 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 5124 274
Size: 4850 570
BsaAI yAC'GTr
Cuts at: 2262 2773 2262
Size: 511 4909
BsaBI GATnn'nnATC
Cuts at: 6 2934 4971 6
Size: 2928 2037 455
Fragments arranged by size:
2928 2037 455
BsaHI Gr'CG_yC
Cuts at: 2851 3099 3801 5297 2851
Size: 248 702 1496 2974
Fragments arranged by size:
2974 1496 702 248
BsaWI w'CCGG_w
Cuts at: 733 1538 2077 2609 3130 3808 4082 4229 4640
Size: 805 539 532 521 678 274 147 411
Cuts at: 4640 733
Size: 1513
Fragments arranged by size:
1513 805 678 539 532 521 411 274
147
BseYI C'CCAG_C
Cuts at: 2845 3603 4180 4676 2845
Size: 758 577 496 3589
Fragments arranged by size:
3589 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1026 2202 1026
Size: 1176 4244
BsiEI CG_ry'CG
Cuts at: 104 2962 3008 4216 104
Size: 2858 46 1208 1308
Fragments arranged by size:
2858 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2434 3212 3402 4194 2434
Size: 778 190 792 3660
Fragments arranged by size:
3660 792 778 190
BsmI GAATG_Cn'
Cuts at: 505 757 841 856 934 1261 1528 1681 1765
Size: 252 84 15 78 327 267 153 84
Cuts at: 1765 1933 505
Size: 168 3992
Fragments arranged by size:
3992 327 267 252 168 153 84 84
78 15
Bsp1286I G_dGCh'C
Cuts at: 366 2434 3045 3138 3212 3402 3464 4194 366
Size: 2068 611 93 74 190 62 730 1592
Fragments arranged by size:
2068 1592 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4640 4640
Size: 5420
BspHI T'CATG_A
Cuts at: 4596 4596
Size: 5420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 302 2033 2470 2684 2986 3367 302
Size: 1731 437 214 302 381 2355
Fragments arranged by size:
2355 1731 437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3712 3766 5250 5415 50
Size: 3662 54 1484 165 55
Fragments arranged by size:
3662 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 678 3332 678
Size: 2654 2766
BsrFI r'CCGG_y
Cuts at: 1538 3418 3599 3808 1538
Size: 1880 181 209 3150
Fragments arranged by size:
3150 1880 209 181
BsrGI T'GTAC_A
Cuts at: 609 609
Size: 5420
BssSI C'ACGA_G
Cuts at: 3691 4049 3691
Size: 358 5062
BstAPI GCAn_nnn'nTGC
Cuts at: 2797 2797
Size: 5420
BstBI TT'CG_AA
Cuts at: 3781 3781
Size: 5420
BstXI CCAn_nnnn'nTGG
Cuts at: 119 1771 119
Size: 1652 3768
BstYI r'GATC_y
Cuts at: 1 789 910 2445 2912 2935 3270 3516 4528
Size: 788 121 1535 467 23 335 246 1012
Cuts at: 4528 4614 4626 4972 1
Size: 86 12 346 449
Fragments arranged by size:
1535 1012 788 467 449 346 335 246
121 86 23 12
BstZ17I GTA'TAC
Cuts at: 799 799
Size: 5420
BtgI C'CryG_G
Cuts at: 358 358
Size: 5420
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3370 3551 3714 3370
Size: 181 163 5076
Fragments arranged by size:
5076 181 163
BtsI GCAGTG_nn'
Cuts at: 364 1266 1938 2190 2663 364
Size: 902 672 252 473 3121
Fragments arranged by size:
3121 902 672 473 252
ClaI AT'CG_AT
Cuts at: 209 209
Size: 5420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 660 695 1500 1535 1668 1703 660
Size: 35 805 35 133 35 4377
Fragments arranged by size:
4377 805 133 35 35 35
DraI TTT'AAA
Cuts at: 666 2419 666
Size: 1753 3667
DraIII CAC_nnn'GTG
Cuts at: 1853 1853
Size: 5420
DrdI GACnn_nn'nnGTC
Cuts at: 3126 3984 3126
Size: 858 4562
EaeI y'GGCC_r
Cuts at: 3005 3179 3570 3597 4942 3005
Size: 174 391 27 1345 3483
Fragments arranged by size:
3483 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3005 3005
Size: 5420
EarI CTCTTCn'nnn_
Cuts at: 715 1854 3443 3653 715
Size: 1139 1589 210 2482
Fragments arranged by size:
2482 1589 1139 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3628 3938 4084 5230 3628
Size: 310 146 1146 3818
Fragments arranged by size:
3818 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2432 2432
Size: 5420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 177 298 341 696 839 1007 1030 1095
Size: 40 121 43 355 143 168 23 65
Cuts at: 1095 1259 1427 1763 1931 2183 2267 2351 3245
Size: 164 168 336 168 252 84 84 894
Cuts at: 3245 3677 4424 4692 137
Size: 432 747 268 865
Fragments arranged by size:
894 865 747 432 355 336 268 252
168 168 168 164 143 121 84 84
65 43 40 23
EcoO109I rG'GnC_Cy
Cuts at: 2559 2559
Size: 5420
EcoRI G'AATT_C
Cuts at: 725 977 1229 1313 1481 1901 2153 2237 2424
Size: 252 252 84 168 420 252 84 187
Cuts at: 2424 725
Size: 3721
Fragments arranged by size:
3721 420 252 252 252 187 168 84
84
EcoRV GAT'ATC
Cuts at: 310 310
Size: 5420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1395 1899 3439 1395
Size: 504 1540 3376
Fragments arranged by size:
3376 1540 504
FspI TGC'GCA
Cuts at: 3201 3201
Size: 5420
HaeII r_GCGC'y
Cuts at: 2854 3102 4124 5103 2854
Size: 248 1022 979 3171
Fragments arranged by size:
3171 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 369 401 902 934 1206 1238 1374
Size: 32 223 32 501 32 272 32 136
Cuts at: 1374 1406 1626 1658 1853 1885 2130 2162 3262
Size: 32 220 32 195 32 245 32 1100
Cuts at: 3262 3292 3294 3324 114
Size: 30 2 30 2210
Fragments arranged by size:
2210 1100 501 272 245 223 220 195
136 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2459 2459
Size: 5420
HindIII A'AGCT_T
Cuts at: 491 491
Size: 5420
KasI G'GCGC_C
Cuts at: 2850 3098 2850
Size: 248 5172
KpnI G_GTAC'C
Cuts at: 2440 2440
Size: 5420
MluI A'CGCG_T
Cuts at: 4853 4853
Size: 5420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 320 1148 4091 4275 320
Size: 828 2943 184 1465
Fragments arranged by size:
2943 1465 828 184
MscI TGG'CCA
Cuts at: 3181 3181
Size: 5420
MslI CAynn'nnrTG
Cuts at: 1002 1058 1072 1424 1769 3409 3536 1002
Size: 56 14 352 345 1640 127 2886
Fragments arranged by size:
2886 1640 352 345 127 56 14
MspA1I CmG'CkG
Cuts at: 2531 2845 3205 4218 4463 2531
Size: 314 360 1013 245 3488
Fragments arranged by size:
3488 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3601 3601
Size: 5420
NarI GG'CG_CC
Cuts at: 2851 3099 2851
Size: 248 5172
NcoI C'CATG_G
Cuts at: 358 358
Size: 5420
NdeI CA'TA_TG
Cuts at: 647 999 1171 647
Size: 352 172 4896
Fragments arranged by size:
4896 352 172
NgoMIV G'CCGG_C
Cuts at: 3599 3599
Size: 5420
NsiI A_TGCA'T
Cuts at: 589 887 1345 589
Size: 298 458 4664
Fragments arranged by size:
4664 458 298
NspI r_CATG'y
Cuts at: 2473 2973 3880 2473
Size: 500 907 4013
Fragments arranged by size:
4013 907 500
PciI A'CATG_T
Cuts at: 3876 3876
Size: 5420
PmeI GTTT'AAAC
Cuts at: 2419 2419
Size: 5420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 4559
Size: 32 5388
PshAI GACnn'nnGTC
Cuts at: 2127 2127
Size: 5420
PsiI TTA'TAA
Cuts at: 446 782 446
Size: 336 5084
PstI C_TGCA'G
Cuts at: 371 2467 3152 371
Size: 2096 685 2639
Fragments arranged by size:
2639 2096 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5420
PvuII CAG'CTG
Cuts at: 2845 3205 2845
Size: 360 5060
RsrII CG'GwC_CG
Cuts at: 3615 3615
Size: 5420
SacI G_AGCT'C
Cuts at: 2434 2434
Size: 5420
SalI G'TCGA_C
Cuts at: 2457 2457
Size: 5420
SapI GCTCTTCn'nnn_
Cuts at: 1854 3443 3653 1854
Size: 1589 210 3621
Fragments arranged by size:
3621 1589 210
SbfI CC_TGCA'GG
Cuts at: 2467 2467
Size: 5420
ScaI AGT'ACT
Cuts at: 4937 4937
Size: 5420
SfcI C'TryA_G
Cuts at: 33 285 367 1200 2463 3148 4141 4332 33
Size: 252 82 833 1263 685 993 191 1121
Fragments arranged by size:
1263 1121 993 833 685 252 191 82
SfoI GGC'GCC
Cuts at: 2852 3100 2852
Size: 248 5172
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
SmaI CCC'GGG
Cuts at: 2442 2442
Size: 5420
SmlI C'TyrA_G
Cuts at: 877 2575 3490 3982 4244 877
Size: 1698 915 492 262 2053
Fragments arranged by size:
2053 1698 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5420
SphI G_CATG'C
Cuts at: 2473 2973 2473
Size: 500 4920
StuI AGG'CCT
Cuts at: 940 1192 1612 2032 2284 940
Size: 252 420 420 252 4076
Fragments arranged by size:
4076 420 420 252 252
StyI C'CwwG_G
Cuts at: 358 2554 2908 358
Size: 2196 354 2870
Fragments arranged by size:
2870 2196 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 1633 3003 3136 3595 3804 541
Size: 1092 1370 133 459 209 2157
Fragments arranged by size:
2157 1370 1092 459 209 133
TatI w'GTAC_w
Cuts at: 546 609 1174 4935 546
Size: 63 565 3761 1031
Fragments arranged by size:
3761 1031 565 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4937 4969 4937
Size: 32 5388
Tth111I GACn'n_nGTC
Cuts at: 3217 3217
Size: 5420
XbaI T'CTAG_A
Cuts at: 2451 2451
Size: 5420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2915 2915
Size: 5420
XmaI C'CCGG_G
Cuts at: 2440 2440
Size: 5420
XmnI GAAnn'nnTTC
Cuts at: 772 1108 1276 1528 1780 2200 2368 772
Size: 336 168 252 252 420 168 3824
Fragments arranged by size:
3824 420 336 252 252 168 168
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BglI BmtI BsaXI BseRI BsiWI BsmBI BssHII
BstEII Bsu36I EcoNI FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI