Restriction map for
pF1KB6268
(Circular) (Six-base) MAPSORT of: pF1KB6268.seq Check: 9927 from: 1 to: 5429
pF1KB6268 5429 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1701 1701
Size: 5429
AatII G_ACGT'C
Cuts at: 2135 2135
Size: 5429
AccI GT'mk_AC
Cuts at: 227 680 2467 227
Size: 453 1787 3189
Fragments arranged by size:
3189 1787 453
Acc65I G'GTAC_C
Cuts at: 2445 2445
Size: 5429
AclI AA'CG_TT
Cuts at: 5269 5269
Size: 5429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 1052 1054 1370 1990 3254 3686 4433 4701
Size: 462 2 316 620 1264 432 747 268
Cuts at: 4701 590
Size: 1318
Fragments arranged by size:
1318 1264 747 620 462 432 316 268
2
AflIII A'CryG_T
Cuts at: 2781 3885 4862 2781
Size: 1104 977 3348
Fragments arranged by size:
3348 1104 977
AgeI A'CCGG_T
Cuts at: 3817 3817
Size: 5429
AhdI GACnn_n'nnGTC
Cuts at: 615 615
Size: 5429
AleI CACnn'nnGTG
Cuts at: 797 797
Size: 5429
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2398 2430 2398
Size: 32 5397
AlwNI CAG_nnn'CTG
Cuts at: 613 2734 4301 613
Size: 2121 1567 1741
Fragments arranged by size:
2121 1741 1567
ApaLI G'TGCA_C
Cuts at: 4199 4199
Size: 5429
ApoI r'AATT_y
Cuts at: 1303 1698 2433 4782 5038 1303
Size: 395 735 2349 256 1694
Fragments arranged by size:
2349 1694 735 395 256
AscI GG'CGCG_CC
Cuts at: 151 151
Size: 5429
AseI AT'TA_AT
Cuts at: 20 1903 20
Size: 1883 3546
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5429
AvaI C'yCGr_G
Cuts at: 401 523 772 1609 2015 2449 401
Size: 122 249 837 406 434 3381
Fragments arranged by size:
3381 837 434 406 249 122
AvrII C'CTAG_G
Cuts at: 2344 2344
Size: 5429
BamHI G'GATC_C
Cuts at: 2454 2454
Size: 5429
BanI G'GyrC_C
Cuts at: 1533 2310 2445 2859 3107 3142 1533
Size: 777 135 414 248 35 3820
Fragments arranged by size:
3820 777 414 248 135 35
BanII G_rGCy'C
Cuts at: 524 716 1267 2443 3473 524
Size: 192 551 1176 1030 2480
Fragments arranged by size:
2480 1176 1030 551 192
BbeI G_GCGC'C
Cuts at: 2863 3111 2863
Size: 248 5181
BbsI GAAGACnn'nnnn_
Cuts at: 611 611
Size: 5429
BbvCI CC'TCA_GC
Cuts at: 1339 1339
Size: 5429
BciVI GTATCCnnnnn_n'
Cuts at: 3327 4088 5429 3327
Size: 761 1341 3327
Fragments arranged by size:
3327 1341 761
BclI T'GATC_A
Cuts at: 1866 2949 1866
Size: 1083 4346
BfrBI ATG'CAT
Cuts at: 1772 1772
Size: 5429
BglI GCCn_nnn'nGGC
Cuts at: 112 2308 112
Size: 2196 3233
BglII A'GATC_T
Cuts at: 2944 4981 2944
Size: 2037 3392
BlpI GC'TnA_GC
Cuts at: 353 2541 353
Size: 2188 3241
Bme1580I G_kGCm'C
Cuts at: 2304 3054 3147 4203 2304
Size: 750 93 1056 3530
Fragments arranged by size:
3530 1056 750 93
BmrI ACTGGGnnnn_n'
Cuts at: 254 312 1270 1694 2641 2722 3057 5201 254
Size: 58 958 424 947 81 335 2144 482
Fragments arranged by size:
2144 958 947 482 424 335 81 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 912 3796 912
Size: 2884 2545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1205 1526 718
Size: 487 321 4621
Fragments arranged by size:
4621 487 321
Bpu10I CC'TnA_GC
Cuts at: 1339 1339
Size: 5429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2605 3484 3976 4274 2605
Size: 879 492 298 3760
Fragments arranged by size:
3760 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 1094 1166 5133 522
Size: 572 72 3967 818
Fragments arranged by size:
3967 818 572 72
BsaAI yAC'GTr
Cuts at: 2782 2782
Size: 5429
BsaBI GATnn'nnATC
Cuts at: 6 2943 4980 6
Size: 2937 2037 455
Fragments arranged by size:
2937 2037 455
BsaHI Gr'CG_yC
Cuts at: 2012 2132 2860 3108 3810 5306 2012
Size: 120 728 248 702 1496 2135
Fragments arranged by size:
2135 1496 728 702 248 120
BsaWI w'CCGG_w
Cuts at: 2135 2618 3139 3817 4091 4238 4649 2135
Size: 483 521 678 274 147 411 2915
Fragments arranged by size:
2915 678 521 483 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2159 2189 2398 2428 2159
Size: 30 209 30 5160
Fragments arranged by size:
5160 209 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 439 573 715 955 1297 1832 2154 2180
Size: 3 134 142 240 342 535 322 26
Cuts at: 2180 2281 436
Size: 101 3584
Fragments arranged by size:
3584 535 342 322 240 142 134 101
26 3
BseYI C'CCAG_C
Cuts at: 2257 2854 3612 4189 4685 2257
Size: 597 758 577 496 3001
Fragments arranged by size:
3001 758 597 577 496
BsiEI CG_ry'CG
Cuts at: 104 2971 3017 4225 104
Size: 2867 46 1208 1308
Fragments arranged by size:
2867 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 524 842 2443 3221 3411 4203 524
Size: 318 1601 778 190 792 1750
Fragments arranged by size:
1750 1601 792 778 318 190
Bsp1286I G_dGCh'C
Cuts at: 524 716 842 1267 2304 2443 3054 3147 3221
Size: 192 126 425 1037 139 611 93 74
Cuts at: 3221 3411 3473 4203 524
Size: 190 62 730 1750
Fragments arranged by size:
1750 1037 730 611 425 192 190 139
126 93 74 62
BspEI T'CCGG_A
Cuts at: 2135 4649 2135
Size: 2514 2915
BspHI T'CATG_A
Cuts at: 552 4605 552
Size: 4053 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1701 2006 2479 2693 2995 3376 1701
Size: 305 473 214 302 381 3754
Fragments arranged by size:
3754 473 381 305 302 214
BsrBI CCG'CTC
Cuts at: 50 3721 3775 5259 5424 50
Size: 3671 54 1484 165 55
Fragments arranged by size:
3671 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 759 1774 3341 759
Size: 1015 1567 2847
Fragments arranged by size:
2847 1567 1015
BsrFI r'CCGG_y
Cuts at: 1500 2271 3427 3608 3817 1500
Size: 771 1156 181 209 3112
Fragments arranged by size:
3112 1156 771 209 181
BsrGI T'GTAC_A
Cuts at: 1858 1858
Size: 5429
BssHII G'CGCG_C
Cuts at: 151 151
Size: 5429
BssSI C'ACGA_G
Cuts at: 280 2126 3700 4058 280
Size: 1846 1574 358 1651
Fragments arranged by size:
1846 1651 1574 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2806 2806
Size: 5429
BstBI TT'CG_AA
Cuts at: 3790 3790
Size: 5429
BstXI CCAn_nnnn'nTGG
Cuts at: 736 736
Size: 5429
BstYI r'GATC_y
Cuts at: 1 691 2454 2921 2944 3279 3525 4537 4623
Size: 690 1763 467 23 335 246 1012 86
Cuts at: 4623 4635 4981 1
Size: 12 346 449
Fragments arranged by size:
1763 1012 690 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 960 2264 960
Size: 1304 4125
BtgI C'CryG_G
Cuts at: 107 461 807 869 1391 2213 107
Size: 354 346 62 522 822 3323
Fragments arranged by size:
3323 822 522 354 346 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3379 3560 3723 3379
Size: 181 163 5085
Fragments arranged by size:
5085 181 163
BtsI GCAGTG_nn'
Cuts at: 1597 2304 2672 1597
Size: 707 368 4354
Fragments arranged by size:
4354 707 368
ClaI AT'CG_AT
Cuts at: 1796 1796
Size: 5429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1380 1415 1380
Size: 35 5394
DraI TTT'AAA
Cuts at: 2428 2428
Size: 5429
DraIII CAC_nnn'GTG
Cuts at: 540 2128 540
Size: 1588 3841
DrdI GACnn_nn'nnGTC
Cuts at: 3135 3993 3135
Size: 858 4571
EaeI y'GGCC_r
Cuts at: 158 630 2114 2381 3014 3188 3579 3606 4951
Size: 472 1484 267 633 174 391 27 1345
Cuts at: 4951 158
Size: 636
Fragments arranged by size:
1484 1345 636 633 472 391 267 174
27
EagI C'GGCC_G
Cuts at: 3014 3014
Size: 5429
EarI CTCTTCn'nnn_
Cuts at: 373 609 1656 1964 3452 3662 373
Size: 236 1047 308 1488 210 2140
Fragments arranged by size:
2140 1488 1047 308 236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 2223 3637 3947 4093 5239 130
Size: 2093 1414 310 146 1146 320
Fragments arranged by size:
2093 1414 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 522 2441 522
Size: 1919 3510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 718 1052 1054 1205 1370 1526 1990 3254
Size: 128 334 2 151 165 156 464 1264
Cuts at: 3254 3686 4433 4701 590
Size: 432 747 268 1318
Fragments arranged by size:
1318 1264 747 464 432 334 268 165
156 151 128 2
EcoNI CCTnn'n_nnAGG
Cuts at: 415 700 740 415
Size: 285 40 5104
Fragments arranged by size:
5104 285 40
EcoO109I rG'GnC_Cy
Cuts at: 306 927 2568 306
Size: 621 1641 3167
Fragments arranged by size:
3167 1641 621
EcoRI G'AATT_C
Cuts at: 1303 1698 2433 1303
Size: 395 735 4299
Fragments arranged by size:
4299 735 395
EcoRV GAT'ATC
Cuts at: 1619 1619
Size: 5429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2115 3448 2115
Size: 1333 4096
FspI TGC'GCA
Cuts at: 3210 3210
Size: 5429
HaeII r_GCGC'y
Cuts at: 127 399 2863 3111 4133 5112 127
Size: 272 2464 248 1022 979 444
Fragments arranged by size:
2464 1022 979 444 272 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 829 861 1240 1272 1603 1635 1963
Size: 32 196 32 379 32 331 32 328
Cuts at: 1963 1995 2159 2191 3271 3301 3303 3333 601
Size: 32 164 32 1080 30 2 30 2697
Fragments arranged by size:
2697 1080 379 331 328 196 164 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 681 2106 2468 681
Size: 1425 362 3642
Fragments arranged by size:
3642 1425 362
HindIII A'AGCT_T
Cuts at: 294 2396 294
Size: 2102 3327
KasI G'GCGC_C
Cuts at: 2859 3107 2859
Size: 248 5181
KpnI G_GTAC'C
Cuts at: 2449 2449
Size: 5429
MluI A'CGCG_T
Cuts at: 4862 4862
Size: 5429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 270 4100 4284 270
Size: 3830 184 1415
Fragments arranged by size:
3830 1415 184
MscI TGG'CCA
Cuts at: 632 2116 2383 3190 632
Size: 1484 267 807 2871
Fragments arranged by size:
2871 1484 807 267
MslI CAynn'nnrTG
Cuts at: 284 551 752 797 3418 3545 284
Size: 267 201 45 2621 127 2168
Fragments arranged by size:
2621 2168 267 201 127 45
MspA1I CmG'CkG
Cuts at: 1872 2025 2173 2540 2854 3214 4227 4472 1872
Size: 153 148 367 314 360 1013 245 2829
Fragments arranged by size:
2829 1013 367 360 314 245 153 148
NaeI GCC'GGC
Cuts at: 1502 2273 3610 1502
Size: 771 1337 3321
Fragments arranged by size:
3321 1337 771
NarI GG'CG_CC
Cuts at: 2860 3108 2860
Size: 248 5181
NcoI C'CATG_G
Cuts at: 107 807 2213 107
Size: 700 1406 3323
Fragments arranged by size:
3323 1406 700
NgoMIV G'CCGG_C
Cuts at: 1500 2271 3608 1500
Size: 771 1337 3321
Fragments arranged by size:
3321 1337 771
NsiI A_TGCA'T
Cuts at: 1774 1774
Size: 5429
NspI r_CATG'y
Cuts at: 289 2065 2482 2982 3889 289
Size: 1776 417 500 907 1829
Fragments arranged by size:
1829 1776 907 500 417
PciI A'CATG_T
Cuts at: 3885 3885
Size: 5429
PflMI CCAn_nnn'nTGG
Cuts at: 135 135
Size: 5429
PmeI GTTT'AAAC
Cuts at: 2428 2428
Size: 5429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2430 4568 4600 2398
Size: 32 2138 32 3227
Fragments arranged by size:
3227 2138 32 32
PpuMI rG'GwC_Cy
Cuts at: 306 306
Size: 5429
PspXI vC'TCGA_Gb
Cuts at: 1609 1609
Size: 5429
PstI C_TGCA'G
Cuts at: 149 416 2476 3161 149
Size: 267 2060 685 2417
Fragments arranged by size:
2417 2060 685 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5429
PvuII CAG'CTG
Cuts at: 1872 2173 2854 3214 1872
Size: 301 681 360 4087
Fragments arranged by size:
4087 681 360 301
RsrII CG'GwC_CG
Cuts at: 3624 3624
Size: 5429
SacI G_AGCT'C
Cuts at: 524 2443 524
Size: 1919 3510
SalI G'TCGA_C
Cuts at: 679 2466 679
Size: 1787 3642
SapI GCTCTTCn'nnn_
Cuts at: 373 3452 3662 373
Size: 3079 210 2140
Fragments arranged by size:
3079 2140 210
SbfI CC_TGCA'GG
Cuts at: 2476 2476
Size: 5429
ScaI AGT'ACT
Cuts at: 1781 2036 4946 1781
Size: 255 2910 2264
Fragments arranged by size:
2910 2264 255
SfcI C'TryA_G
Cuts at: 33 145 412 477 687 2472 3157 4150 4341
Size: 112 267 65 210 1785 685 993 191
Cuts at: 4341 33
Size: 1121
Fragments arranged by size:
1785 1121 993 685 267 210 191 112
65
SfoI GGC'GCC
Cuts at: 2861 3109 2861
Size: 248 5181
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5429
SmaI CCC'GGG
Cuts at: 2451 2451
Size: 5429
SmlI C'TyrA_G
Cuts at: 523 1609 2584 3499 3991 4253 523
Size: 1086 975 915 492 262 1699
Fragments arranged by size:
1699 1086 975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5429
SphI G_CATG'C
Cuts at: 2482 2982 2482
Size: 500 4929
SspI AAT'ATT
Cuts at: 193 193
Size: 5429
StuI AGG'CCT
Cuts at: 895 895
Size: 5429
StyI C'CwwG_G
Cuts at: 107 807 1184 1472 2213 2344 2563 2917 107
Size: 700 377 288 741 131 219 354 2619
Fragments arranged by size:
2619 741 700 377 354 288 219 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 787 3012 3145 3604 3813 787
Size: 2225 133 459 209 2403
Fragments arranged by size:
2403 2225 459 209 133
TatI w'GTAC_w
Cuts at: 1779 1858 2034 4944 1779
Size: 79 176 2910 2264
Fragments arranged by size:
2910 2264 176 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 676 708 832 864 4946 4978 676
Size: 32 124 32 4082 32 1127
Fragments arranged by size:
4082 1127 124 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3226 3226
Size: 5429
XbaI T'CTAG_A
Cuts at: 2460 2460
Size: 5429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 2924 309
Size: 2615 2814
XhoI C'TCGA_G
Cuts at: 523 1609 523
Size: 1086 4343
XmaI C'CCGG_G
Cuts at: 2449 2449
Size: 5429
XmnI GAAnn'nnTTC
Cuts at: 1698 2410 1698
Size: 712 4717
ZraI GAC'GTC
Cuts at: 2133 2133
Size: 5429
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI ApaI BaeI BcgI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BstEII BstZ17I FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI