Restriction map for
pF1KB6272
(Circular) (Six-base) MAPSORT of: pF1KB6272.seq Check: 7223 from: 1 to: 5432
pF1KB6272 5432 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2470 2470
Size: 5432
Acc65I G'GTAC_C
Cuts at: 2448 2448
Size: 5432
AclI AA'CG_TT
Cuts at: 1197 5272 1197
Size: 4075 1357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 944 3257 3689 4436 4704 944
Size: 2313 432 747 268 1672
Fragments arranged by size:
2313 1672 747 432 268
AflIII A'CryG_T
Cuts at: 2784 3888 4865 2784
Size: 1104 977 3351
Fragments arranged by size:
3351 1104 977
AgeI A'CCGG_T
Cuts at: 3820 3820
Size: 5432
AhdI GACnn_n'nnGTC
Cuts at: 669 669
Size: 5432
AleI CACnn'nnGTG
Cuts at: 902 1082 902
Size: 180 5252
AlwNI CAG_nnn'CTG
Cuts at: 1175 2737 4304 1175
Size: 1562 1567 2303
Fragments arranged by size:
2303 1567 1562
ApaI G_GGCC'C
Cuts at: 1633 1633
Size: 5432
ApaLI G'TGCA_C
Cuts at: 4202 4202
Size: 5432
ApoI r'AATT_y
Cuts at: 2436 4785 5041 2436
Size: 2349 256 2827
Fragments arranged by size:
2827 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
AvaI C'yCGr_G
Cuts at: 276 2138 2452 276
Size: 1862 314 3256
Fragments arranged by size:
3256 1862 314
BamHI G'GATC_C
Cuts at: 2457 2457
Size: 5432
BanI G'GyrC_C
Cuts at: 141 2448 2862 3110 3145 141
Size: 2307 414 248 35 2428
Fragments arranged by size:
2428 2307 414 248 35
BanII G_rGCy'C
Cuts at: 736 1099 1633 1717 2186 2446 3476 736
Size: 363 534 84 469 260 1030 2692
Fragments arranged by size:
2692 1030 534 469 363 260 84
BbeI G_GCGC'C
Cuts at: 2866 3114 2866
Size: 248 5184
BbsI GAAGACnn'nnnn_
Cuts at: 572 1234 572
Size: 662 4770
BbvCI CC'TCA_GC
Cuts at: 1221 1221
Size: 5432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1382 1416 1382
Size: 34 5398
BciVI GTATCCnnnnn_n'
Cuts at: 3330 4091 5432 3330
Size: 761 1341 3330
Fragments arranged by size:
3330 1341 761
BclI T'GATC_A
Cuts at: 2952 2952
Size: 5432
BfrBI ATG'CAT
Cuts at: 417 1668 417
Size: 1251 4181
BglII A'GATC_T
Cuts at: 2056 2947 4984 2056
Size: 891 2037 2504
Fragments arranged by size:
2504 2037 891
BlpI GC'TnA_GC
Cuts at: 2544 2544
Size: 5432
Bme1580I G_kGCm'C
Cuts at: 1633 3057 3150 4206 1633
Size: 1424 93 1056 2859
Fragments arranged by size:
2859 1424 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 846 2644 2725 3060 5204 846
Size: 1798 81 335 2144 1074
Fragments arranged by size:
2144 1798 1074 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3799 3799
Size: 5432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 270 929 1086 1394 1397 2296 145
Size: 125 659 157 308 3 899 3281
Fragments arranged by size:
3281 899 659 308 157 125 3
Bpu10I CC'TnA_GC
Cuts at: 579 1221 1716 579
Size: 642 495 4295
Fragments arranged by size:
4295 642 495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 2608 3487 3979 4277 2194
Size: 414 879 492 298 3349
Fragments arranged by size:
3349 879 492 414 298
BsaI GGTCTCn'nnnn_
Cuts at: 5136 5136
Size: 5432
BsaAI yAC'GTr
Cuts at: 2785 2785
Size: 5432
BsaBI GATnn'nnATC
Cuts at: 6 1073 2946 4983 6
Size: 1067 1873 2037 455
Fragments arranged by size:
2037 1873 1067 455
BsaHI Gr'CG_yC
Cuts at: 2863 3111 3813 5309 2863
Size: 248 702 1496 2986
Fragments arranged by size:
2986 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1613 1830 2621 3142 3820 4094 4241 4652 1613
Size: 217 791 521 678 274 147 411 2393
Fragments arranged by size:
2393 791 678 521 411 274 217 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 292
Size: 30 5402
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 1089 1209 1457 1923 139
Size: 950 120 248 466 3648
Fragments arranged by size:
3648 950 466 248 120
BseYI C'CCAG_C
Cuts at: 373 2857 3615 4192 4688 373
Size: 2484 758 577 496 1117
Fragments arranged by size:
2484 1117 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 2218 221
Size: 1997 3435
BsiEI CG_ry'CG
Cuts at: 104 2190 2974 3020 4228 104
Size: 2086 784 46 1208 1308
Fragments arranged by size:
2086 1308 1208 784 46
BsiHKAI G_wGCw'C
Cuts at: 1099 2186 2446 3224 3414 4206 1099
Size: 1087 260 778 190 792 2325
Fragments arranged by size:
2325 1087 792 778 260 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1532 2393 1532
Size: 861 4571
Bsp1286I G_dGCh'C
Cuts at: 736 1099 1633 1717 2186 2446 3057 3150 3224
Size: 363 534 84 469 260 611 93 74
Cuts at: 3224 3414 3476 4206 736
Size: 190 62 730 1962
Fragments arranged by size:
1962 730 611 534 469 363 260 190
93 84 74 62
BspEI T'CCGG_A
Cuts at: 1613 4652 1613
Size: 3039 2393
BspHI T'CATG_A
Cuts at: 4608 4608
Size: 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1988 2482 2696 2998 3379 1988
Size: 494 214 302 381 4041
Fragments arranged by size:
4041 494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2118 3724 3778 5262 5427 50
Size: 2068 1606 54 1484 165 55
Fragments arranged by size:
2068 1606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3344 3344
Size: 5432
BsrFI r'CCGG_y
Cuts at: 1357 1729 2377 3430 3611 3820 1357
Size: 372 648 1053 181 209 2969
Fragments arranged by size:
2969 1053 648 372 209 181
BssSI C'ACGA_G
Cuts at: 3703 4061 3703
Size: 358 5074
BstAPI GCAn_nnn'nTGC
Cuts at: 985 2809 985
Size: 1824 3608
BstBI TT'CG_AA
Cuts at: 933 3793 933
Size: 2860 2572
BstXI CCAn_nnnn'nTGG
Cuts at: 381 1270 381
Size: 889 4543
BstYI r'GATC_y
Cuts at: 1 2056 2457 2924 2947 3282 3528 4540 4626
Size: 2055 401 467 23 335 246 1012 86
Cuts at: 4626 4638 4984 1
Size: 12 346 449
Fragments arranged by size:
2055 1012 467 449 401 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 441 441
Size: 5432
BtgI C'CryG_G
Cuts at: 908 1700 908
Size: 792 4640
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 499 3382 3563 3726 499
Size: 2883 181 163 2205
Fragments arranged by size:
2883 2205 181 163
BtsI GCAGTG_nn'
Cuts at: 513 983 1089 1631 2675 513
Size: 470 106 542 1044 3270
Fragments arranged by size:
3270 1044 542 470 106
DraI TTT'AAA
Cuts at: 228 2431 228
Size: 2203 3229
DrdI GACnn_nn'nnGTC
Cuts at: 3138 3996 3138
Size: 858 4574
EaeI y'GGCC_r
Cuts at: 1071 2126 2187 2258 2375 3017 3191 3582 3609
Size: 1055 61 71 117 642 174 391 27
Cuts at: 3609 4954 1071
Size: 1345 1549
Fragments arranged by size:
1549 1345 1055 642 391 174 117 71
61 27
EagI C'GGCC_G
Cuts at: 2187 3017 2187
Size: 830 4602
EarI CTCTTCn'nnn_
Cuts at: 899 1152 1299 1817 2333 3455 3665 899
Size: 253 147 518 516 1122 210 2666
Fragments arranged by size:
2666 1122 518 516 253 210 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3640 3950 4096 5242 3640
Size: 310 146 1146 3830
Fragments arranged by size:
3830 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1097 2184 2444 1097
Size: 1087 260 4085
Fragments arranged by size:
4085 1087 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 270 929 944 1086 1394 1397 2296 3257
Size: 125 659 15 142 308 3 899 961
Cuts at: 3257 3689 4436 4704 145
Size: 432 747 268 873
Fragments arranged by size:
961 899 873 747 659 432 308 268
142 125 15 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1895 2248 1895
Size: 353 5079
EcoO109I rG'GnC_Cy
Cuts at: 293 584 663 1543 1769 1887 2079 2571 293
Size: 291 79 880 226 118 192 492 3154
Fragments arranged by size:
3154 880 492 291 226 192 118 79
EcoRI G'AATT_C
Cuts at: 2436 2436
Size: 5432
EcoRV GAT'ATC
Cuts at: 2034 2034
Size: 5432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5432
FseI GG_CCGG'CC
Cuts at: 2381 2381
Size: 5432
FspI TGC'GCA
Cuts at: 3213 3213
Size: 5432
HaeII r_GCGC'y
Cuts at: 2866 3114 4136 5115 2866
Size: 248 1022 979 3183
Fragments arranged by size:
3183 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 670 687 702 1395 1427 1742 1774 1939
Size: 15 17 15 693 32 315 32 165
Cuts at: 1939 1971 1978 2010 2023 2055 2332 2364 3274
Size: 32 7 32 13 32 277 32 910
Cuts at: 3274 3304 3306 3336 655
Size: 30 2 30 2751
Fragments arranged by size:
2751 910 693 315 277 165 32 32
32 32 32 32 30 30 17 15
15 13 7 2
HincII GTy'rAC
Cuts at: 2471 2471
Size: 5432
KasI G'GCGC_C
Cuts at: 2862 3110 2862
Size: 248 5184
KpnI G_GTAC'C
Cuts at: 2452 2452
Size: 5432
MluI A'CGCG_T
Cuts at: 4865 4865
Size: 5432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4103 4287 4103
Size: 184 5248
MscI TGG'CCA
Cuts at: 1073 2128 3193 1073
Size: 1055 1065 3312
Fragments arranged by size:
3312 1065 1055
MslI CAynn'nnrTG
Cuts at: 808 902 1082 1190 1268 1642 3421 3548 808
Size: 94 180 108 78 374 1779 127 2692
Fragments arranged by size:
2692 1779 374 180 127 108 94 78
MspA1I CmG'CkG
Cuts at: 1492 2543 2857 3217 4230 4475 1492
Size: 1051 314 360 1013 245 2449
Fragments arranged by size:
2449 1051 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1359 2379 3613 1359
Size: 1020 1234 3178
Fragments arranged by size:
3178 1234 1020
NarI GG'CG_CC
Cuts at: 2863 3111 2863
Size: 248 5184
NcoI C'CATG_G
Cuts at: 908 1700 908
Size: 792 4640
NgoMIV G'CCGG_C
Cuts at: 1357 2377 3611 1357
Size: 1020 1234 3178
Fragments arranged by size:
3178 1234 1020
NsiI A_TGCA'T
Cuts at: 419 1670 419
Size: 1251 4181
NspI r_CATG'y
Cuts at: 254 1668 1905 2485 2985 3892 254
Size: 1414 237 580 500 907 1794
Fragments arranged by size:
1794 1414 907 580 500 237
PciI A'CATG_T
Cuts at: 3888 3888
Size: 5432
PflMI CCAn_nnn'nTGG
Cuts at: 1626 2256 2389 1626
Size: 630 133 4669
Fragments arranged by size:
4669 630 133
PfoI T'CCnGG_A
Cuts at: 286 616 286
Size: 330 5102
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 259 291 322 354 4571 4603 259
Size: 32 31 32 4217 32 1088
Fragments arranged by size:
4217 1088 32 32 32 31
PpuMI rG'GwC_Cy
Cuts at: 584 663 1543 1769 1887 2079 584
Size: 79 880 226 118 192 3937
Fragments arranged by size:
3937 880 226 192 118 79
PspOMI G'GGCC_C
Cuts at: 1629 1629
Size: 5432
PspXI vC'TCGA_Gb
Cuts at: 276 276
Size: 5432
PstI C_TGCA'G
Cuts at: 2479 3164 2479
Size: 685 4747
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5432
PvuII CAG'CTG
Cuts at: 1492 2857 3217 1492
Size: 1365 360 3707
Fragments arranged by size:
3707 1365 360
RsrII CG'GwC_CG
Cuts at: 3627 3627
Size: 5432
SacI G_AGCT'C
Cuts at: 1099 2186 2446 1099
Size: 1087 260 4085
Fragments arranged by size:
4085 1087 260
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5432
SanDI GG'GwC_CC
Cuts at: 1769 1769
Size: 5432
SapI GCTCTTCn'nnn_
Cuts at: 3455 3665 3455
Size: 210 5222
SbfI CC_TGCA'GG
Cuts at: 2479 2479
Size: 5432
ScaI AGT'ACT
Cuts at: 856 4949 856
Size: 4093 1339
SexAI A'CCwGG_T
Cuts at: 843 1823 843
Size: 980 4452
SfcI C'TryA_G
Cuts at: 33 1176 1915 2475 3160 4153 4344 33
Size: 1143 739 560 685 993 191 1121
Fragments arranged by size:
1143 1121 993 739 685 560 191
SfoI GGC'GCC
Cuts at: 2864 3112 2864
Size: 248 5184
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
SmaI CCC'GGG
Cuts at: 2454 2454
Size: 5432
SmlI C'TyrA_G
Cuts at: 276 2209 2587 3502 3994 4256 276
Size: 1933 378 915 492 262 1452
Fragments arranged by size:
1933 1452 915 492 378 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5432
SphI G_CATG'C
Cuts at: 1905 2485 2985 1905
Size: 580 500 4352
Fragments arranged by size:
4352 580 500
StuI AGG'CCT
Cuts at: 891 1519 891
Size: 628 4804
StyI C'CwwG_G
Cuts at: 296 390 666 792 908 1090 1700 2372 2566
Size: 94 276 126 116 182 610 672 194
Cuts at: 2566 2920 296
Size: 354 2808
Fragments arranged by size:
2808 672 610 354 276 194 182 126
116 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1693 3015 3148 3607 3816 1693
Size: 1322 133 459 209 3309
Fragments arranged by size:
3309 1322 459 209 133
TatI w'GTAC_w
Cuts at: 854 4947 854
Size: 4093 1339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 354 386 1761 1793 4949 4981 354
Size: 32 1375 32 3156 32 805
Fragments arranged by size:
3156 1375 805 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3229 3229
Size: 5432
XbaI T'CTAG_A
Cuts at: 1501 2463 1501
Size: 962 4470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 1707 1780 2927 294
Size: 1413 73 1147 2799
Fragments arranged by size:
2799 1413 1147 73
XhoI C'TCGA_G
Cuts at: 276 276
Size: 5432
XmaI C'CCGG_G
Cuts at: 2452 2452
Size: 5432
XmnI GAAnn'nnTTC
Cuts at: 828 828
Size: 5432
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BglI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PshAI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI