Restriction map for
pF1KB6274
(Circular) (Six-base) MAPSORT of: pF1KB6274.seq Check: 783 from: 1 to: 5204
pF1KB6274 5204 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2242 2242
Size: 5204
Acc65I G'GTAC_C
Cuts at: 2220 2220
Size: 5204
AclI AA'CG_TT
Cuts at: 5044 5044
Size: 5204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 820 1143 1570 3029 3461 4208 4476 721
Size: 99 323 427 1459 432 747 268 1449
Fragments arranged by size:
1459 1449 747 432 427 323 268 99
AfeI AGC'GCT
Cuts at: 1114 2066 1114
Size: 952 4252
AflIII A'CryG_T
Cuts at: 1308 2556 3660 4637 1308
Size: 1248 1104 977 1875
Fragments arranged by size:
1875 1248 1104 977
AgeI A'CCGG_T
Cuts at: 3592 3592
Size: 5204
AleI CACnn'nnGTG
Cuts at: 1297 2131 1297
Size: 834 4370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2174 2174
Size: 5204
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1913 1945 1913
Size: 32 5172
AlwNI CAG_nnn'CTG
Cuts at: 260 297 370 1912 2185 2509 4076 260
Size: 37 73 1542 273 324 1567 1388
Fragments arranged by size:
1567 1542 1388 324 273 73 37
ApaI G_GGCC'C
Cuts at: 124 382 1063 124
Size: 258 681 4265
Fragments arranged by size:
4265 681 258
ApaLI G'TGCA_C
Cuts at: 481 520 1934 2149 3974 481
Size: 39 1414 215 1825 1711
Fragments arranged by size:
1825 1711 1414 215 39
ApoI r'AATT_y
Cuts at: 343 2208 4557 4813 343
Size: 1865 2349 256 734
Fragments arranged by size:
2349 1865 734 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5204
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5204
AvaI C'yCGr_G
Cuts at: 1630 1844 2224 1630
Size: 214 380 4610
Fragments arranged by size:
4610 380 214
BamHI G'GATC_C
Cuts at: 2229 2229
Size: 5204
BanI G'GyrC_C
Cuts at: 584 912 2058 2220 2634 2882 2917 584
Size: 328 1146 162 414 248 35 2871
Fragments arranged by size:
2871 1146 414 328 248 162 35
BanII G_rGCy'C
Cuts at: 124 180 382 609 1063 1225 1235 1472 1906
Size: 56 202 227 454 162 10 237 434
Cuts at: 1906 2218 3248 124
Size: 312 1030 2080
Fragments arranged by size:
2080 1030 454 434 312 237 227 202
162 56 10
BbeI G_GCGC'C
Cuts at: 588 916 2062 2638 2886 588
Size: 328 1146 576 248 2906
Fragments arranged by size:
2906 1146 576 328 248
BbsI GAAGACnn'nnnn_
Cuts at: 745 1617 745
Size: 872 4332
BbvCI CC'TCA_GC
Cuts at: 1577 1577
Size: 5204
BciVI GTATCCnnnnn_n'
Cuts at: 3102 3863 5204 3102
Size: 761 1341 3102
Fragments arranged by size:
3102 1341 761
BclI T'GATC_A
Cuts at: 2724 2724
Size: 5204
BglI GCCn_nnn'nGGC
Cuts at: 2015 2024 2015
Size: 9 5195
BglII A'GATC_T
Cuts at: 1247 2719 4756 1247
Size: 1472 2037 1695
Fragments arranged by size:
2037 1695 1472
BlpI GC'TnA_GC
Cuts at: 826 2316 826
Size: 1490 3714
Bme1580I G_kGCm'C
Cuts at: 124 382 485 524 548 1063 1938 2153 2192
Size: 258 103 39 24 515 875 215 39
Cuts at: 2192 2829 2922 3978 124
Size: 637 93 1056 1350
Fragments arranged by size:
1350 1056 875 637 515 258 215 103
93 39 39 24
BmgBI CAC'GTC
Cuts at: 1263 1263
Size: 5204
BmrI ACTGGGnnnn_n'
Cuts at: 855 1971 2173 2416 2497 2832 4976 855
Size: 1116 202 243 81 335 2144 1083
Fragments arranged by size:
2144 1116 1083 335 243 202 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3571 3571
Size: 5204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 988 2060 616
Size: 372 1072 3760
Fragments arranged by size:
3760 1072 372
Bpu10I CC'TnA_GC
Cuts at: 1577 1905 1577
Size: 328 4876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 2380 3259 3751 4049 892
Size: 1488 879 492 298 2047
Fragments arranged by size:
2047 1488 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 682 775 4908 682
Size: 93 4133 978
Fragments arranged by size:
4133 978 93
BsaAI yAC'GTr
Cuts at: 630 2133 2557 630
Size: 1503 424 3277
Fragments arranged by size:
3277 1503 424
BsaBI GATnn'nnATC
Cuts at: 6 2718 4755 6
Size: 2712 2037 455
Fragments arranged by size:
2712 2037 455
BsaHI Gr'CG_yC
Cuts at: 585 893 913 1140 2059 2635 2883 3585 5081
Size: 308 20 227 919 576 248 702 1496
Cuts at: 5081 585
Size: 708
Fragments arranged by size:
1496 919 708 702 576 308 248 227
20
BsaWI w'CCGG_w
Cuts at: 851 2393 2914 3592 3866 4013 4424 851
Size: 1542 521 678 274 147 411 1631
Fragments arranged by size:
1631 1542 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 841 871 943 973 1780 1810 445
Size: 30 366 30 72 30 807 30 3839
Fragments arranged by size:
3839 807 366 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 964 991 1801 1804 457
Size: 507 27 810 3 3857
Fragments arranged by size:
3857 810 507 27 3
BseYI C'CCAG_C
Cuts at: 370 1320 1361 1452 2629 3387 3964 4460 370
Size: 950 41 91 1177 758 577 496 1114
Fragments arranged by size:
1177 1114 950 758 577 496 91 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 1129 1275 246
Size: 883 146 4175
Fragments arranged by size:
4175 883 146
BsiEI CG_ry'CG
Cuts at: 104 1344 2746 2792 4000 104
Size: 1240 1402 46 1208 1308
Fragments arranged by size:
1402 1308 1240 1208 46
BsiHKAI G_wGCw'C
Cuts at: 485 524 1235 1938 2153 2218 2996 3186 3978
Size: 39 711 703 215 65 778 190 792
Cuts at: 3978 485
Size: 1711
Fragments arranged by size:
1711 792 778 711 703 215 190 65
39
BsmBI CGTCTCn'nnnn_
Cuts at: 900 900
Size: 5204
Bsp1286I G_dGCh'C
Cuts at: 124 180 382 485 524 548 609 1063 1225
Size: 56 202 103 39 24 61 454 162
Cuts at: 1225 1235 1472 1906 1938 2153 2192 2218 2829
Size: 10 237 434 32 215 39 26 611
Cuts at: 2829 2922 2996 3186 3248 3978 124
Size: 93 74 190 62 730 1350
Fragments arranged by size:
1350 730 611 454 434 237 215 202
190 162 103 93 74 62 61 56
39 39 32 26 24 10
BspEI T'CCGG_A
Cuts at: 4424 4424
Size: 5204
BspHI T'CATG_A
Cuts at: 4380 4380
Size: 5204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2254 2468 2770 3151 2254
Size: 214 302 381 4307
Fragments arranged by size:
4307 381 302 214
BsrBI CCG'CTC
Cuts at: 50 226 1047 1968 3496 3550 5034 5199 50
Size: 176 821 921 1528 54 1484 165 55
Fragments arranged by size:
1528 1484 921 821 176 165 55 54
BsrDI GCAATG_nn'
Cuts at: 307 1685 3116 307
Size: 1378 1431 2395
Fragments arranged by size:
2395 1431 1378
BsrFI r'CCGG_y
Cuts at: 524 821 972 1343 3202 3383 3592 524
Size: 297 151 371 1859 181 209 2136
Fragments arranged by size:
2136 1859 371 297 209 181 151
BssSI C'ACGA_G
Cuts at: 3475 3833 3475
Size: 358 4846
BstAPI GCAn_nnn'nTGC
Cuts at: 260 297 497 1652 2581 260
Size: 37 200 1155 929 2883
Fragments arranged by size:
2883 1155 929 200 37
BstBI TT'CG_AA
Cuts at: 3565 3565
Size: 5204
BstXI CCAn_nnnn'nTGG
Cuts at: 1702 1702
Size: 5204
BstYI r'GATC_y
Cuts at: 1 599 623 1247 1985 2229 2696 2719 3054
Size: 598 24 624 738 244 467 23 335
Cuts at: 3054 3300 4312 4398 4410 4756 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 738 624 598 467 449 346 335
246 244 86 24 23 12
BtgI C'CryG_G
Cuts at: 107 579 917 1853 107
Size: 472 338 936 3458
Fragments arranged by size:
3458 936 472 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3154 3335 3498 3154
Size: 181 163 4860
Fragments arranged by size:
4860 181 163
BtsI GCAGTG_nn'
Cuts at: 258 2447 258
Size: 2189 3015
DraI TTT'AAA
Cuts at: 2203 2203
Size: 5204
DrdI GACnn_nn'nnGTC
Cuts at: 1267 2086 2910 3768 1267
Size: 819 824 858 2703
Fragments arranged by size:
2703 858 824 819
EaeI y'GGCC_r
Cuts at: 977 1208 1341 1856 2016 2093 2789 2963 3354
Size: 231 133 515 160 77 696 174 391
Cuts at: 3354 3381 4726 977
Size: 27 1345 1455
Fragments arranged by size:
1455 1345 696 515 391 231 174 160
133 77 27
EagI C'GGCC_G
Cuts at: 1341 2789 1341
Size: 1448 3756
EarI CTCTTCn'nnn_
Cuts at: 1071 1516 3227 3437 1071
Size: 445 1711 210 2838
Fragments arranged by size:
2838 1711 445 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 406 3412 3722 3868 5014 406
Size: 3006 310 146 1146 596
Fragments arranged by size:
3006 1146 596 310 146
EcoICRI GAG'CTC
Cuts at: 1233 2216 1233
Size: 983 4221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 721 820 988 1143 1570 2060 3029 3461
Size: 105 99 168 155 427 490 969 432
Cuts at: 3461 4208 4476 616
Size: 747 268 1344
Fragments arranged by size:
1344 969 747 490 432 427 268 168
155 105 99
EcoO109I rG'GnC_Cy
Cuts at: 121 148 294 378 379 1060 1333 1545 1658
Size: 27 146 84 1 681 273 212 113
Cuts at: 1658 1909 2343 121
Size: 251 434 2982
Fragments arranged by size:
2982 681 434 273 251 212 146 113
84 27 1
EcoRI G'AATT_C
Cuts at: 2208 2208
Size: 5204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1134 3223 1134
Size: 2089 3115
FseI GG_CCGG'CC
Cuts at: 1347 1347
Size: 5204
FspI TGC'GCA
Cuts at: 2985 2985
Size: 5204
HaeII r_GCGC'y
Cuts at: 371 531 579 588 822 916 1116 1321 1899
Size: 160 48 9 234 94 200 205 578
Cuts at: 1899 2062 2068 2638 2886 3908 4887 371
Size: 163 6 570 248 1022 979 688
Fragments arranged by size:
1022 979 688 578 570 248 234 205
200 163 160 94 48 9 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 591 623 841 873 1708 1740 1970 1977 2002
Size: 32 218 32 835 32 230 7 25
Cuts at: 2002 2009 3046 3076 3078 3108 591
Size: 7 1037 30 2 30 2687
Fragments arranged by size:
2687 1037 835 230 218 32 32 32
30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 2243 2243
Size: 5204
KasI G'GCGC_C
Cuts at: 584 912 2058 2634 2882 584
Size: 328 1146 576 248 2906
Fragments arranged by size:
2906 1146 576 328 248
KpnI G_GTAC'C
Cuts at: 2224 2224
Size: 5204
MluI A'CGCG_T
Cuts at: 4637 4637
Size: 5204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1178 3875 4059 1178
Size: 2697 184 2323
Fragments arranged by size:
2697 2323 184
MscI TGG'CCA
Cuts at: 1858 2095 2965 1858
Size: 237 870 4097
Fragments arranged by size:
4097 870 237
MslI CAynn'nnrTG
Cuts at: 1297 2131 2156 3193 3320 1297
Size: 834 25 1037 127 3181
Fragments arranged by size:
3181 1037 834 127 25
MspA1I CmG'CkG
Cuts at: 581 594 1452 1952 2024 2315 2629 2989 4002
Size: 13 858 500 72 291 314 360 1013
Cuts at: 4002 4247 581
Size: 245 1538
Fragments arranged by size:
1538 1013 858 500 360 314 291 245
72 13
NaeI GCC'GGC
Cuts at: 823 1345 3385 823
Size: 522 2040 2642
Fragments arranged by size:
2642 2040 522
NarI GG'CG_CC
Cuts at: 585 913 2059 2635 2883 585
Size: 328 1146 576 248 2906
Fragments arranged by size:
2906 1146 576 328 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5204
NgoMIV G'CCGG_C
Cuts at: 821 1343 3383 821
Size: 522 2040 2642
Fragments arranged by size:
2642 2040 522
NspI r_CATG'y
Cuts at: 1611 2257 2757 3664 1611
Size: 646 500 907 3151
Fragments arranged by size:
3151 907 646 500
PasI CC'CwG_GG
Cuts at: 1888 1888
Size: 5204
PciI A'CATG_T
Cuts at: 3660 3660
Size: 5204
PflMI CCAn_nnn'nTGG
Cuts at: 370 370
Size: 5204
PfoI T'CCnGG_A
Cuts at: 1134 1271 1134
Size: 137 5067
PmeI GTTT'AAAC
Cuts at: 2203 2203
Size: 5204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 4343
Size: 32 5172
PpuMI rG'GwC_Cy
Cuts at: 148 1333 1545 1658 148
Size: 1185 212 113 3694
Fragments arranged by size:
3694 1185 212 113
PspOMI G'GGCC_C
Cuts at: 120 378 1059 120
Size: 258 681 4265
Fragments arranged by size:
4265 681 258
PstI C_TGCA'G
Cuts at: 256 316 593 997 2251 2936 256
Size: 60 277 404 1254 685 2524
Fragments arranged by size:
2524 1254 685 404 277 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5204
PvuII CAG'CTG
Cuts at: 594 1452 2629 2989 594
Size: 858 1177 360 2809
Fragments arranged by size:
2809 1177 858 360
RsrII CG'GwC_CG
Cuts at: 1389 3399 1389
Size: 2010 3194
SacI G_AGCT'C
Cuts at: 1235 2218 1235
Size: 983 4221
SacII CC_GC'GG
Cuts at: 582 582
Size: 5204
SalI G'TCGA_C
Cuts at: 2241 2241
Size: 5204
SanDI GG'GwC_CC
Cuts at: 148 1658 148
Size: 1510 3694
SapI GCTCTTCn'nnn_
Cuts at: 3227 3437 3227
Size: 210 4994
SbfI CC_TGCA'GG
Cuts at: 2251 2251
Size: 5204
ScaI AGT'ACT
Cuts at: 4721 4721
Size: 5204
SfcI C'TryA_G
Cuts at: 33 252 312 429 589 993 1539 2247 2932
Size: 219 60 117 160 404 546 708 685
Cuts at: 2932 3925 4116 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 708 685 546 404 219 191
160 117 60
SfoI GGC'GCC
Cuts at: 586 914 2060 2636 2884 586
Size: 328 1146 576 248 2906
Fragments arranged by size:
2906 1146 576 328 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5204
SgrAI Cr'CCGG_yG
Cuts at: 524 524
Size: 5204
SmaI CCC'GGG
Cuts at: 1632 1846 2226 1632
Size: 214 380 4610
Fragments arranged by size:
4610 380 214
SmlI C'TyrA_G
Cuts at: 907 2359 3274 3766 4028 907
Size: 1452 915 492 262 2083
Fragments arranged by size:
2083 1452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5204
SphI G_CATG'C
Cuts at: 2257 2757 2257
Size: 500 4704
StyI C'CwwG_G
Cuts at: 107 2338 2692 107
Size: 2231 354 2619
Fragments arranged by size:
2619 2231 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1375 1901 2787 2920 3379 3588 1375
Size: 526 886 133 459 209 2991
Fragments arranged by size:
2991 886 526 459 209 133
TatI w'GTAC_w
Cuts at: 4719 4719
Size: 5204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 4721 4753 445
Size: 32 4244 32 896
Fragments arranged by size:
4244 896 32 32
Tth111I GACn'n_nGTC
Cuts at: 3001 3001
Size: 5204
XbaI T'CTAG_A
Cuts at: 2235 2235
Size: 5204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 634 2699 114
Size: 520 2065 2619
Fragments arranged by size:
2619 2065 520
XmaI C'CCGG_G
Cuts at: 1630 1844 2224 1630
Size: 214 380 4610
Fragments arranged by size:
4610 380 214
XmnI GAAnn'nnTTC
Cuts at: 1624 1624
Size: 5204
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI ZraI