Restriction map for
pF1KB6278
(Circular) (Six-base) MAPSORT of: pF1KB6278.seq Check: 9097 from: 1 to: 5456
pF1KB6278 5456 bp
With 182 enzymes: SgfI *
December 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1603 1603
Size: 5456
AccI GT'mk_AC
Cuts at: 1707 2494 1707
Size: 787 4669
Acc65I G'GTAC_C
Cuts at: 1942 2472 1942
Size: 530 4926
AclI AA'CG_TT
Cuts at: 1501 5296 1501
Size: 3795 1661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 665 1187 1195 1262 1408 1799 3281 3713
Size: 145 522 8 67 146 391 1482 432
Cuts at: 3713 4460 4728 520
Size: 747 268 1248
Fragments arranged by size:
1482 1248 747 522 432 391 268 146
145 67 8
AflII C'TTAA_G
Cuts at: 1833 1833
Size: 5456
AflIII A'CryG_T
Cuts at: 2808 3912 4889 2808
Size: 1104 977 3375
Fragments arranged by size:
3375 1104 977
AgeI A'CCGG_T
Cuts at: 3844 3844
Size: 5456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2266 2266
Size: 5456
AlwNI CAG_nnn'CTG
Cuts at: 2431 2761 4328 2431
Size: 330 1567 3559
Fragments arranged by size:
3559 1567 330
ApaI G_GGCC'C
Cuts at: 369 369
Size: 5456
ApaLI G'TGCA_C
Cuts at: 4226 4226
Size: 5456
ApoI r'AATT_y
Cuts at: 540 716 1533 2146 2460 4809 5065 540
Size: 176 817 613 314 2349 256 931
Fragments arranged by size:
2349 931 817 613 314 256 176
AseI AT'TA_AT
Cuts at: 20 977 1067 20
Size: 957 90 4409
Fragments arranged by size:
4409 957 90
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
AvaI C'yCGr_G
Cuts at: 945 2476 945
Size: 1531 3925
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 890 923 890
Size: 33 5423
BamHI G'GATC_C
Cuts at: 2481 2481
Size: 5456
BanI G'GyrC_C
Cuts at: 1942 2472 2886 3134 3169 1942
Size: 530 414 248 35 4229
Fragments arranged by size:
4229 530 414 248 35
BanII G_rGCy'C
Cuts at: 369 2028 2470 3500 369
Size: 1659 442 1030 2325
Fragments arranged by size:
2325 1659 1030 442
BbeI G_GCGC'C
Cuts at: 2890 3138 2890
Size: 248 5208
BbsI GAAGACnn'nnnn_
Cuts at: 2014 2014
Size: 5456
BciVI GTATCCnnnnn_n'
Cuts at: 3354 4115 5456 3354
Size: 761 1341 3354
Fragments arranged by size:
3354 1341 761
BclI T'GATC_A
Cuts at: 2976 2976
Size: 5456
BglII A'GATC_T
Cuts at: 1206 2971 5008 1206
Size: 1765 2037 1654
Fragments arranged by size:
2037 1765 1654
BlpI GC'TnA_GC
Cuts at: 2568 2568
Size: 5456
Bme1580I G_kGCm'C
Cuts at: 369 3081 3174 4230 369
Size: 2712 93 1056 1595
Fragments arranged by size:
2712 1595 1056 93
BmgBI CAC'GTC
Cuts at: 159 159
Size: 5456
BmrI ACTGGGnnnn_n'
Cuts at: 2668 2749 3084 5228 2668
Size: 81 335 2144 2896
Fragments arranged by size:
2896 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3823 3823
Size: 5456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1619 2066 1619
Size: 447 5009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2632 3511 4003 4301 2632
Size: 879 492 298 3787
Fragments arranged by size:
3787 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5160 5160
Size: 5456
BsaAI yAC'GTr
Cuts at: 2809 2809
Size: 5456
BsaBI GATnn'nnATC
Cuts at: 6 2970 5007 6
Size: 2964 2037 455
Fragments arranged by size:
2964 2037 455
BsaHI Gr'CG_yC
Cuts at: 1576 2887 3135 3837 5333 1576
Size: 1311 248 702 1496 1699
Fragments arranged by size:
1699 1496 1311 702 248
BsaWI w'CCGG_w
Cuts at: 2645 3166 3844 4118 4265 4676 2645
Size: 521 678 274 147 411 3425
Fragments arranged by size:
3425 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 427 457 1617 1647 427
Size: 30 1160 30 4236
Fragments arranged by size:
4236 1160 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 358 431 355
Size: 3 73 5380
Fragments arranged by size:
5380 73 3
BseYI C'CCAG_C
Cuts at: 2881 3639 4216 4712 2881
Size: 758 577 496 3625
Fragments arranged by size:
3625 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 389
Size: 5456
BsiEI CG_ry'CG
Cuts at: 104 353 2998 3044 4252 104
Size: 249 2645 46 1208 1308
Fragments arranged by size:
2645 1308 1208 249 46
BsiHKAI G_wGCw'C
Cuts at: 423 2028 2470 3248 3438 4230 423
Size: 1605 442 778 190 792 1649
Fragments arranged by size:
1649 1605 792 778 442 190
BsmI GAATG_Cn'
Cuts at: 1734 2040 1734
Size: 306 5150
Bsp1286I G_dGCh'C
Cuts at: 369 423 2028 2470 3081 3174 3248 3438 3500
Size: 54 1605 442 611 93 74 190 62
Cuts at: 3500 4230 369
Size: 730 1595
Fragments arranged by size:
1605 1595 730 611 442 190 93 74
62 54
BspEI T'CCGG_A
Cuts at: 4676 4676
Size: 5456
BspHI T'CATG_A
Cuts at: 4632 4632
Size: 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 645 1182 1603 2506 2720 3022 3403 310
Size: 335 537 421 903 214 302 381 2363
Fragments arranged by size:
2363 903 537 421 381 335 302 214
BsrBI CCG'CTC
Cuts at: 50 115 282 1050 3748 3802 5286 5451 50
Size: 65 167 768 2698 54 1484 165 55
Fragments arranged by size:
2698 1484 768 167 165 65 55 54
BsrDI GCAATG_nn'
Cuts at: 3368 3368
Size: 5456
BsrFI r'CCGG_y
Cuts at: 3454 3635 3844 3454
Size: 181 209 5066
Fragments arranged by size:
5066 209 181
BsrGI T'GTAC_A
Cuts at: 1290 1290
Size: 5456
BssSI C'ACGA_G
Cuts at: 3727 4085 3727
Size: 358 5098
BstAPI GCAn_nnn'nTGC
Cuts at: 1536 2833 1536
Size: 1297 4159
BstBI TT'CG_AA
Cuts at: 3817 3817
Size: 5456
BstXI CCAn_nnnn'nTGG
Cuts at: 431 431
Size: 5456
BstYI r'GATC_y
Cuts at: 1 632 1206 2481 2948 2971 3306 3552 4564
Size: 631 574 1275 467 23 335 246 1012
Cuts at: 4564 4650 4662 5008 1
Size: 86 12 346 449
Fragments arranged by size:
1275 1012 631 574 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1708 1708
Size: 5456
BtgI C'CryG_G
Cuts at: 195 1371 195
Size: 1176 4280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 308 3406 3587 3750 308
Size: 3098 181 163 2014
Fragments arranged by size:
3098 2014 181 163
BtsI GCAGTG_nn'
Cuts at: 2699 2699
Size: 5456
DraI TTT'AAA
Cuts at: 2455 2455
Size: 5456
DrdI GACnn_nn'nnGTC
Cuts at: 3162 4020 3162
Size: 858 4598
EaeI y'GGCC_r
Cuts at: 192 293 350 3041 3215 3606 3633 4978 192
Size: 101 57 2691 174 391 27 1345 670
Fragments arranged by size:
2691 1345 670 391 174 101 57 27
EagI C'GGCC_G
Cuts at: 350 3041 350
Size: 2691 2765
EarI CTCTTCn'nnn_
Cuts at: 684 1116 1238 1775 1932 3479 3689 684
Size: 432 122 537 157 1547 210 2451
Fragments arranged by size:
2451 1547 537 432 210 157 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2358 3664 3974 4120 5266 2358
Size: 1306 310 146 1146 2548
Fragments arranged by size:
2548 1306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2026 2468 2026
Size: 442 5014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 665 1187 1195 1262 1408 1619 1799 2066
Size: 145 522 8 67 146 211 180 267
Cuts at: 2066 3281 3713 4460 4728 520
Size: 1215 432 747 268 1248
Fragments arranged by size:
1248 1215 747 522 432 268 267 211
180 146 145 67 8
EcoO109I rG'GnC_Cy
Cuts at: 365 366 2595 365
Size: 1 2229 3226
Fragments arranged by size:
3226 2229 1
EcoRI G'AATT_C
Cuts at: 2460 2460
Size: 5456
EcoRV GAT'ATC
Cuts at: 2388 2388
Size: 5456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 3475 2407
Size: 1068 4388
FspI TGC'GCA
Cuts at: 3237 3237
Size: 5456
HaeII r_GCGC'y
Cuts at: 2890 3138 4160 5139 2890
Size: 248 1022 979 3207
Fragments arranged by size:
3207 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 2001 2013 2033 2045 3298 3328 3330
Size: 32 1542 12 20 12 1253 30 2
Cuts at: 3330 3360 427
Size: 30 2523
Fragments arranged by size:
2523 1542 1253 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 2495 2495
Size: 5456
KasI G'GCGC_C
Cuts at: 2886 3134 2886
Size: 248 5208
KpnI G_GTAC'C
Cuts at: 1946 2476 1946
Size: 530 4926
MfeI C'AATT_G
Cuts at: 827 827
Size: 5456
MluI A'CGCG_T
Cuts at: 4889 4889
Size: 5456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4127 4311 4127
Size: 184 5272
MscI TGG'CCA
Cuts at: 3217 3217
Size: 5456
MslI CAynn'nnrTG
Cuts at: 2135 3445 3572 2135
Size: 1310 127 4019
Fragments arranged by size:
4019 1310 127
MspA1I CmG'CkG
Cuts at: 253 1434 2567 2881 3241 4254 4499 253
Size: 1181 1133 314 360 1013 245 1210
Fragments arranged by size:
1210 1181 1133 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3637 3637
Size: 5456
NarI GG'CG_CC
Cuts at: 2887 3135 2887
Size: 248 5208
NcoI C'CATG_G
Cuts at: 1371 1371
Size: 5456
NgoMIV G'CCGG_C
Cuts at: 3635 3635
Size: 5456
NotI GC'GGCC_GC
Cuts at: 350 350
Size: 5456
NspI r_CATG'y
Cuts at: 2509 3009 3916 2509
Size: 500 907 4049
Fragments arranged by size:
4049 907 500
PciI A'CATG_T
Cuts at: 3912 3912
Size: 5456
PflMI CCAn_nnn'nTGG
Cuts at: 799 1990 799
Size: 1191 4265
PfoI T'CCnGG_A
Cuts at: 1440 1719 1440
Size: 279 5177
PmeI GTTT'AAAC
Cuts at: 2455 2455
Size: 5456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 4595
Size: 32 5424
PspOMI G'GGCC_C
Cuts at: 365 365
Size: 5456
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2346 2378 2346
Size: 32 5424
PstI C_TGCA'G
Cuts at: 212 307 972 1746 2298 2503 3188 212
Size: 95 665 774 552 205 685 2480
Fragments arranged by size:
2480 774 685 665 552 205 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5456
PvuII CAG'CTG
Cuts at: 1434 2881 3241 1434
Size: 1447 360 3649
Fragments arranged by size:
3649 1447 360
RsrII CG'GwC_CG
Cuts at: 3651 3651
Size: 5456
SacI G_AGCT'C
Cuts at: 2028 2470 2028
Size: 442 5014
SalI G'TCGA_C
Cuts at: 2493 2493
Size: 5456
SapI GCTCTTCn'nnn_
Cuts at: 684 3479 3689 684
Size: 2795 210 2451
Fragments arranged by size:
2795 2451 210
SbfI CC_TGCA'GG
Cuts at: 2503 2503
Size: 5456
ScaI AGT'ACT
Cuts at: 4973 4973
Size: 5456
SfcI C'TryA_G
Cuts at: 33 208 303 968 1274 1691 1742 2294 2499
Size: 175 95 665 306 417 51 552 205
Cuts at: 2499 3184 4177 4368 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 552 417 306 205
191 175 95 51
SfoI GGC'GCC
Cuts at: 2888 3136 2888
Size: 248 5208
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
SmaI CCC'GGG
Cuts at: 2478 2478
Size: 5456
SmlI C'TyrA_G
Cuts at: 1833 2611 3526 4018 4280 1833
Size: 778 915 492 262 3009
Fragments arranged by size:
3009 915 778 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5456
SphI G_CATG'C
Cuts at: 2509 3009 2509
Size: 500 4956
SspI AAT'ATT
Cuts at: 560 897 1263 2325 560
Size: 337 366 1062 3691
Fragments arranged by size:
3691 1062 366 337
StyI C'CwwG_G
Cuts at: 152 1371 2590 2944 152
Size: 1219 1219 354 2664
Fragments arranged by size:
2664 1219 1219 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3039 3172 3631 3840 3039
Size: 133 459 209 4655
Fragments arranged by size:
4655 459 209 133
TatI w'GTAC_w
Cuts at: 1141 1290 1509 1839 2075 2245 4971 1141
Size: 149 219 330 236 170 2726 1626
Fragments arranged by size:
2726 1626 330 236 219 170 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1352 1384 1617 1649 2064 2096 4973 5005 1352
Size: 32 233 32 415 32 2877 32 1803
Fragments arranged by size:
2877 1803 415 233 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3253 3253
Size: 5456
XbaI T'CTAG_A
Cuts at: 927 2487 927
Size: 1560 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 643 2951 643
Size: 2308 3148
XmaI C'CCGG_G
Cuts at: 2476 2476
Size: 5456
XmnI GAAnn'nnTTC
Cuts at: 765 765
Size: 5456
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PflMI PfoI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI AvrII BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI NdeI NheI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI