Restriction map for
pF1KB6279
(Circular) (Six-base) MAPSORT of: pF1KB6279.seq Check: 4707 from: 1 to: 5495
pF1KB6279 5495 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 214 521 2533 214
Size: 307 2012 3176
Fragments arranged by size:
3176 2012 307
Acc65I G'GTAC_C
Cuts at: 1582 2511 1582
Size: 929 4566
AclI AA'CG_TT
Cuts at: 552 5335 552
Size: 4783 712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1770 1817 1823 3320 3752 4499 4767 733
Size: 1037 47 6 1497 432 747 268 1461
Fragments arranged by size:
1497 1461 1037 747 432 268 47 6
AflIII A'CryG_T
Cuts at: 672 1907 2847 3951 4928 672
Size: 1235 940 1104 977 1239
Fragments arranged by size:
1239 1235 1104 977 940
AgeI A'CCGG_T
Cuts at: 3883 3883
Size: 5495
AleI CACnn'nnGTG
Cuts at: 623 623
Size: 5495
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 653 2075 653
Size: 1422 4073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 335 367 537 569 2288 2320 335
Size: 32 170 32 1719 32 3510
Fragments arranged by size:
3510 1719 170 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1320 1674 1737 1801 2800 4367 1320
Size: 354 63 64 999 1567 2448
Fragments arranged by size:
2448 1567 999 354 64 63
ApaI G_GGCC'C
Cuts at: 1469 1469
Size: 5495
ApaLI G'TGCA_C
Cuts at: 4265 4265
Size: 5495
ApoI r'AATT_y
Cuts at: 1827 2097 2401 2410 2499 4848 5104 1827
Size: 270 304 9 89 2349 256 2218
Fragments arranged by size:
2349 2218 304 270 256 89 9
AseI AT'TA_AT
Cuts at: 20 1292 20
Size: 1272 4223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
AvaI C'yCGr_G
Cuts at: 1983 2515 1983
Size: 532 4963
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 458 491 724 757 1790 1823 458
Size: 33 233 33 1033 33 4130
Fragments arranged by size:
4130 1033 233 33 33 33
BamHI G'GATC_C
Cuts at: 2520 2520
Size: 5495
BanI G'GyrC_C
Cuts at: 1582 2511 2925 3173 3208 1582
Size: 929 414 248 35 3869
Fragments arranged by size:
3869 929 414 248 35
BanII G_rGCy'C
Cuts at: 1234 1469 2290 2509 3539 1234
Size: 235 821 219 1030 3190
Fragments arranged by size:
3190 1030 821 235 219
BbeI G_GCGC'C
Cuts at: 2929 3177 2929
Size: 248 5247
BbsI GAAGACnn'nnnn_
Cuts at: 164 669 164
Size: 505 4990
BbvCI CC'TCA_GC
Cuts at: 2252 2252
Size: 5495
BciVI GTATCCnnnnn_n'
Cuts at: 3393 4154 5495 3393
Size: 761 1341 3393
Fragments arranged by size:
3393 1341 761
BclI T'GATC_A
Cuts at: 1440 3015 1440
Size: 1575 3920
BglII A'GATC_T
Cuts at: 3010 5047 3010
Size: 2037 3458
BlpI GC'TnA_GC
Cuts at: 2607 2607
Size: 5495
Bme1580I G_kGCm'C
Cuts at: 1469 3120 3213 4269 1469
Size: 1651 93 1056 2695
Fragments arranged by size:
2695 1651 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1463 2707 2788 3123 5267 1463
Size: 1244 81 335 2144 1691
Fragments arranged by size:
2144 1691 1244 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3862 3862
Size: 5495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 1342 2102 245
Size: 1097 760 3638
Fragments arranged by size:
3638 1097 760
Bpu10I CC'TnA_GC
Cuts at: 2252 2252
Size: 5495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 2671 3550 4042 4340 395
Size: 2276 879 492 298 1550
Fragments arranged by size:
2276 1550 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5199 5199
Size: 5495
BsaAI yAC'GTr
Cuts at: 2848 2848
Size: 5495
BsaBI GATnn'nnATC
Cuts at: 6 3009 5046 6
Size: 3003 2037 455
Fragments arranged by size:
3003 2037 455
BsaHI Gr'CG_yC
Cuts at: 2926 3174 3876 5372 2926
Size: 248 702 1496 3049
Fragments arranged by size:
3049 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2684 3205 3883 4157 4304 4715 2684
Size: 521 678 274 147 411 3464
Fragments arranged by size:
3464 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 373
Size: 5495
BseYI C'CCAG_C
Cuts at: 2258 2443 2920 3678 4255 4751 2258
Size: 185 477 758 577 496 3002
Fragments arranged by size:
3002 758 577 496 477 185
BsiEI CG_ry'CG
Cuts at: 104 3037 3083 4291 104
Size: 2933 46 1208 1308
Fragments arranged by size:
2933 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 632 2290 2313 2509 3287 3477 4269 632
Size: 1658 23 196 778 190 792 1858
Fragments arranged by size:
1858 1658 792 778 196 190 23
BsmI GAATG_Cn'
Cuts at: 2032 2032
Size: 5495
Bsp1286I G_dGCh'C
Cuts at: 632 1234 1469 2290 2313 2509 3120 3213 3287
Size: 602 235 821 23 196 611 93 74
Cuts at: 3287 3477 3539 4269 632
Size: 190 62 730 1858
Fragments arranged by size:
1858 821 730 611 602 235 196 190
93 74 62 23
BspEI T'CCGG_A
Cuts at: 4715 4715
Size: 5495
BspHI T'CATG_A
Cuts at: 948 1698 4671 948
Size: 750 2973 1772
Fragments arranged by size:
2973 1772 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 752 2043 2545 2759 3061 3442 752
Size: 1291 502 214 302 381 2805
Fragments arranged by size:
2805 1291 502 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3787 3841 5325 5490 50
Size: 3737 54 1484 165 55
Fragments arranged by size:
3737 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 642 1434 3407 642
Size: 792 1973 2730
Fragments arranged by size:
2730 1973 792
BsrFI r'CCGG_y
Cuts at: 3493 3674 3883 3493
Size: 181 209 5105
Fragments arranged by size:
5105 209 181
BssSI C'ACGA_G
Cuts at: 3766 4124 3766
Size: 358 5137
BstAPI GCAn_nnn'nTGC
Cuts at: 1198 2872 1198
Size: 1674 3821
BstBI TT'CG_AA
Cuts at: 3856 3856
Size: 5495
BstEII G'GTnAC_C
Cuts at: 1848 1848
Size: 5495
BstXI CCAn_nnnn'nTGG
Cuts at: 183 2485 183
Size: 2302 3193
BstYI r'GATC_y
Cuts at: 1 275 2520 2987 3010 3345 3591 4603 4689
Size: 274 2245 467 23 335 246 1012 86
Cuts at: 4689 4701 5047 1
Size: 12 346 449
Fragments arranged by size:
2245 1012 467 449 346 335 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 1646 1646
Size: 5495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3445 3626 3789 3445
Size: 181 163 5151
Fragments arranged by size:
5151 181 163
BtsI GCAGTG_nn'
Cuts at: 630 1039 2354 2738 630
Size: 409 1315 384 3387
Fragments arranged by size:
3387 1315 409 384
DraI TTT'AAA
Cuts at: 2494 2494
Size: 5495
DrdI GACnn_nn'nnGTC
Cuts at: 2053 3201 4059 2053
Size: 1148 858 3489
Fragments arranged by size:
3489 1148 858
EaeI y'GGCC_r
Cuts at: 207 2475 3080 3254 3645 3672 5017 207
Size: 2268 605 174 391 27 1345 685
Fragments arranged by size:
2268 1345 685 605 391 174 27
EagI C'GGCC_G
Cuts at: 3080 3080
Size: 5495
EarI CTCTTCn'nnn_
Cuts at: 374 1355 2043 3518 3728 374
Size: 981 688 1475 210 2141
Fragments arranged by size:
2141 1475 981 688 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3703 4013 4159 5305 3703
Size: 310 146 1146 3893
Fragments arranged by size:
3893 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2288 2507 2288
Size: 219 5276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 733 1342 1770 1817 1823 2102 3320 3752
Size: 488 609 428 47 6 279 1218 432
Cuts at: 3752 4499 4767 245
Size: 747 268 973
Fragments arranged by size:
1218 973 747 609 488 432 428 279
268 47 6
EcoO109I rG'GnC_Cy
Cuts at: 1465 2634 1465
Size: 1169 4326
EcoRI G'AATT_C
Cuts at: 2097 2499 2097
Size: 402 5093
EcoRV GAT'ATC
Cuts at: 332 1781 332
Size: 1449 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1003 2148 3514 1003
Size: 1145 1366 2984
Fragments arranged by size:
2984 1366 1145
FspI TGC'GCA
Cuts at: 3276 3276
Size: 5495
HaeII r_GCGC'y
Cuts at: 2929 3177 4199 5178 2929
Size: 248 1022 979 3246
Fragments arranged by size:
3246 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 574 606 959 991 1060 1092 3337
Size: 32 226 32 353 32 69 32 2245
Cuts at: 3337 3367 3369 3399 316
Size: 30 2 30 2412
Fragments arranged by size:
2412 2245 353 226 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2534 2534
Size: 5495
HindIII A'AGCT_T
Cuts at: 2273 2273
Size: 5495
KasI G'GCGC_C
Cuts at: 2925 3173 2925
Size: 248 5247
KpnI G_GTAC'C
Cuts at: 1586 2515 1586
Size: 929 4566
MluI A'CGCG_T
Cuts at: 4928 4928
Size: 5495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1721 1848 4166 4350 1721
Size: 127 2318 184 2866
Fragments arranged by size:
2866 2318 184 127
MscI TGG'CCA
Cuts at: 209 2477 3256 209
Size: 2268 779 2448
Fragments arranged by size:
2448 2268 779
MslI CAynn'nnrTG
Cuts at: 623 635 689 705 773 3484 3611 623
Size: 12 54 16 68 2711 127 2507
Fragments arranged by size:
2711 2507 127 68 54 16 12
MspA1I CmG'CkG
Cuts at: 1618 2606 2920 3280 4293 4538 1618
Size: 988 314 360 1013 245 2575
Fragments arranged by size:
2575 1013 988 360 314 245
NaeI GCC'GGC
Cuts at: 3676 3676
Size: 5495
NarI GG'CG_CC
Cuts at: 2926 3174 2926
Size: 248 5247
NdeI CA'TA_TG
Cuts at: 830 1356 830
Size: 526 4969
NgoMIV G'CCGG_C
Cuts at: 3674 3674
Size: 5495
NspI r_CATG'y
Cuts at: 676 726 1911 2548 3048 3955 676
Size: 50 1185 637 500 907 2216
Fragments arranged by size:
2216 1185 907 637 500 50
PciI A'CATG_T
Cuts at: 672 1907 3951 672
Size: 1235 2044 2216
Fragments arranged by size:
2216 2044 1235
PfoI T'CCnGG_A
Cuts at: 175 175
Size: 5495
PmeI GTTT'AAAC
Cuts at: 2494 2494
Size: 5495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 4634
Size: 32 5463
PspOMI G'GGCC_C
Cuts at: 1465 1465
Size: 5495
PstI C_TGCA'G
Cuts at: 288 1733 1937 2542 3227 288
Size: 1445 204 605 685 2556
Fragments arranged by size:
2556 1445 685 605 204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5495
PvuII CAG'CTG
Cuts at: 1618 2920 3280 1618
Size: 1302 360 3833
Fragments arranged by size:
3833 1302 360
RsrII CG'GwC_CG
Cuts at: 3690 3690
Size: 5495
SacI G_AGCT'C
Cuts at: 2290 2509 2290
Size: 219 5276
SalI G'TCGA_C
Cuts at: 2532 2532
Size: 5495
SapI GCTCTTCn'nnn_
Cuts at: 374 3518 3728 374
Size: 3144 210 2141
Fragments arranged by size:
3144 2141 210
SbfI CC_TGCA'GG
Cuts at: 2542 2542
Size: 5495
ScaI AGT'ACT
Cuts at: 245 1164 1491 5012 245
Size: 919 327 3521 728
Fragments arranged by size:
3521 919 728 327
SfcI C'TryA_G
Cuts at: 33 232 284 418 1729 1933 2538 3223 4216
Size: 199 52 134 1311 204 605 685 993
Cuts at: 4216 4407 33
Size: 191 1121
Fragments arranged by size:
1311 1121 993 685 605 204 199 191
134 52
SfoI GGC'GCC
Cuts at: 2927 3175 2927
Size: 248 5247
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
SmaI CCC'GGG
Cuts at: 2517 2517
Size: 5495
SmlI C'TyrA_G
Cuts at: 410 2650 3565 4057 4319 410
Size: 2240 915 492 262 1586
Fragments arranged by size:
2240 1586 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5495
SphI G_CATG'C
Cuts at: 2548 3048 2548
Size: 500 4995
StyI C'CwwG_G
Cuts at: 2417 2629 2983 2417
Size: 212 354 4929
Fragments arranged by size:
4929 354 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 184 1280 3078 3211 3670 3879 184
Size: 1096 1798 133 459 209 1800
Fragments arranged by size:
1800 1798 1096 459 209 133
TatI w'GTAC_w
Cuts at: 243 1162 1489 5010 243
Size: 919 327 3521 728
Fragments arranged by size:
3521 919 728 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 419 451 5012 5044 419
Size: 32 4561 32 870
Fragments arranged by size:
4561 870 32 32
Tth111I GACn'n_nGTC
Cuts at: 3292 3292
Size: 5495
XbaI T'CTAG_A
Cuts at: 2526 2526
Size: 5495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1762 2990 1762
Size: 1228 4267
XmaI C'CCGG_G
Cuts at: 2515 2515
Size: 5495
XmnI GAAnn'nnTTC
Cuts at: 404 2097 404
Size: 1693 3802
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BcgI
BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI