Restriction map for
pF1KB6281
(Circular) (Six-base) MAPSORT of: pF1KB6281.seq Check: 3888 from: 1 to: 5507
pF1KB6281 5507 bp
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AatII G_ACGT'C
Cuts at: 723 723
Size: 5507
AccI GT'mk_AC
Cuts at: 2545 2545
Size: 5507
Acc65I G'GTAC_C
Cuts at: 2523 2523
Size: 5507
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 2062 3332 3764 4511 4779 1075
Size: 987 1270 432 747 268 1803
Fragments arranged by size:
1803 1270 987 747 432 268
AflII C'TTAA_G
Cuts at: 538 957 538
Size: 419 5088
AflIII A'CryG_T
Cuts at: 119 2859 3963 4940 119
Size: 2740 1104 977 686
Fragments arranged by size:
2740 1104 977 686
AgeI A'CCGG_T
Cuts at: 1549 3895 1549
Size: 2346 3161
AhdI GACnn_n'nnGTC
Cuts at: 138 138
Size: 5507
AlwNI CAG_nnn'CTG
Cuts at: 2812 4379 2812
Size: 1567 3940
ApaLI G'TGCA_C
Cuts at: 2223 4277 2223
Size: 2054 3453
ApoI r'AATT_y
Cuts at: 666 1611 1950 2511 4860 5116 666
Size: 945 339 561 2349 256 1057
Fragments arranged by size:
2349 1057 945 561 339 256
AseI AT'TA_AT
Cuts at: 20 1277 1301 20
Size: 1257 24 4226
Fragments arranged by size:
4226 1257 24
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 2527 2527
Size: 5507
BamHI G'GATC_C
Cuts at: 2532 2532
Size: 5507
BanI G'GyrC_C
Cuts at: 499 555 1077 2523 2937 3185 3220 499
Size: 56 522 1446 414 248 35 2786
Fragments arranged by size:
2786 1446 522 414 248 56 35
BanII G_rGCy'C
Cuts at: 1319 2521 3551 1319
Size: 1202 1030 3275
Fragments arranged by size:
3275 1202 1030
BbeI G_GCGC'C
Cuts at: 2941 3189 2941
Size: 248 5259
BbsI GAAGACnn'nnnn_
Cuts at: 188 422 1106 1228 188
Size: 234 684 122 4467
Fragments arranged by size:
4467 684 234 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 182 1153 1187 148
Size: 34 971 34 4468
Fragments arranged by size:
4468 971 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 240 2305 3405 4166 5507 240
Size: 2065 1100 761 1341 240
Fragments arranged by size:
2065 1341 1100 761 240
BclI T'GATC_A
Cuts at: 3027 3027
Size: 5507
BglII A'GATC_T
Cuts at: 201 3022 5059 201
Size: 2821 2037 649
Fragments arranged by size:
2821 2037 649
BlpI GC'TnA_GC
Cuts at: 2619 2619
Size: 5507
Bme1580I G_kGCm'C
Cuts at: 2227 3132 3225 4281 2227
Size: 905 93 1056 3453
Fragments arranged by size:
3453 1056 905 93
BmgBI CAC'GTC
Cuts at: 456 456
Size: 5507
BmrI ACTGGGnnnn_n'
Cuts at: 1229 1508 2719 2800 3135 5279 1229
Size: 279 1211 81 335 2144 1457
Fragments arranged by size:
2144 1457 1211 335 279 81
BmtI G_CTAG'C
Cuts at: 376 376
Size: 5507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3874 3874
Size: 5507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1862 1862
Size: 5507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 2683 3562 4054 4352 256
Size: 2427 879 492 298 1411
Fragments arranged by size:
2427 1411 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 601 1443 1880 5211 601
Size: 842 437 3331 897
Fragments arranged by size:
3331 897 842 437
BsaAI yAC'GTr
Cuts at: 2860 2860
Size: 5507
BsaBI GATnn'nnATC
Cuts at: 6 3021 5058 6
Size: 3015 2037 455
Fragments arranged by size:
3015 2037 455
BsaHI Gr'CG_yC
Cuts at: 720 2938 3186 3888 5384 720
Size: 2218 248 702 1496 843
Fragments arranged by size:
2218 1496 843 702 248
BsaWI w'CCGG_w
Cuts at: 1549 2696 3217 3895 4169 4316 4727 1549
Size: 1147 521 678 274 147 411 2329
Fragments arranged by size:
2329 1147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 990 1020 1301 1331 990
Size: 30 281 30 5166
Fragments arranged by size:
5166 281 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 758 995 998 1344 2233 758
Size: 237 3 346 889 4032
Fragments arranged by size:
4032 889 346 237 3
BseYI C'CCAG_C
Cuts at: 2932 3690 4267 4763 2932
Size: 758 577 496 3676
Fragments arranged by size:
3676 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 300
Size: 5507
BsiEI CG_ry'CG
Cuts at: 104 3049 3095 4303 104
Size: 2945 46 1208 1308
Fragments arranged by size:
2945 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1319 2227 2521 3299 3489 4281 1319
Size: 908 294 778 190 792 2545
Fragments arranged by size:
2545 908 792 778 294 190
BsmI GAATG_Cn'
Cuts at: 592 592
Size: 5507
BsmBI CGTCTCn'nnnn_
Cuts at: 446 536 446
Size: 90 5417
Bsp1286I G_dGCh'C
Cuts at: 1319 2227 2521 3132 3225 3299 3489 3551 4281
Size: 908 294 611 93 74 190 62 730
Cuts at: 4281 1319
Size: 2545
Fragments arranged by size:
2545 908 730 611 294 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4727 4727
Size: 5507
BspHI T'CATG_A
Cuts at: 4683 4683
Size: 5507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1425 2315 2557 2771 3073 3454 1425
Size: 890 242 214 302 381 3478
Fragments arranged by size:
3478 890 381 302 242 214
BsrBI CCG'CTC
Cuts at: 50 3799 3853 5337 5502 50
Size: 3749 54 1484 165 55
Fragments arranged by size:
3749 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3419 3419
Size: 5507
BsrFI r'CCGG_y
Cuts at: 1549 3505 3686 3895 1549
Size: 1956 181 209 3161
Fragments arranged by size:
3161 1956 209 181
BssSI C'ACGA_G
Cuts at: 1210 2032 3778 4136 1210
Size: 822 1746 358 2581
Fragments arranged by size:
2581 1746 822 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2884 2884
Size: 5507
BstBI TT'CG_AA
Cuts at: 3868 3868
Size: 5507
BstEII G'GTnAC_C
Cuts at: 1252 1252
Size: 5507
BstYI r'GATC_y
Cuts at: 1 201 1888 1900 2532 2999 3022 3357 3603
Size: 200 1687 12 632 467 23 335 246
Cuts at: 3603 4615 4701 4713 5059 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1687 1012 632 467 449 346 335 246
200 86 23 12 12
BtgI C'CryG_G
Cuts at: 107 1625 107
Size: 1518 3989
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 288 597 2750 288
Size: 309 2153 3045
Fragments arranged by size:
3045 2153 309
DraI TTT'AAA
Cuts at: 2506 2506
Size: 5507
DrdI GACnn_nn'nnGTC
Cuts at: 900 3213 4071 900
Size: 2313 858 2336
Fragments arranged by size:
2336 2313 858
EaeI y'GGCC_r
Cuts at: 3092 3266 3657 3684 5029 3092
Size: 174 391 27 1345 3570
Fragments arranged by size:
3570 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3092 3092
Size: 5507
EarI CTCTTCn'nnn_
Cuts at: 347 518 682 890 1899 2038 2123 3530 3740
Size: 171 164 208 1009 139 85 1407 210
Cuts at: 3740 347
Size: 2114
Fragments arranged by size:
2114 1407 1009 210 208 171 164 139
85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3715 4025 4171 5317 3715
Size: 310 146 1146 3905
Fragments arranged by size:
3905 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1317 2519 1317
Size: 1202 4305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1862 2062 3332 3764 4511 4779 1075
Size: 787 200 1270 432 747 268 1803
Fragments arranged by size:
1803 1270 787 747 432 268 200
EcoO109I rG'GnC_Cy
Cuts at: 1817 2646 1817
Size: 829 4678
EcoRI G'AATT_C
Cuts at: 666 2511 666
Size: 1845 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 564 3526 564
Size: 2962 2545
FspI TGC'GCA
Cuts at: 2323 2497 3288 2323
Size: 174 791 4542
Fragments arranged by size:
4542 791 174
HaeII r_GCGC'y
Cuts at: 2136 2941 3189 4211 5190 2136
Size: 805 248 1022 979 2453
Fragments arranged by size:
2453 1022 979 805 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 681 713 1339 1371 1744 1776 1791
Size: 32 525 32 626 32 373 32 15
Cuts at: 1791 1823 2392 2424 3349 3379 3381 3411 124
Size: 32 569 32 925 30 2 30 2220
Fragments arranged by size:
2220 925 626 569 525 373 32 32
32 32 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 700 2546 700
Size: 1846 3661
HindIII A'AGCT_T
Cuts at: 2490 2490
Size: 5507
KasI G'GCGC_C
Cuts at: 2937 3185 2937
Size: 248 5259
KpnI G_GTAC'C
Cuts at: 2527 2527
Size: 5507
MfeI C'AATT_G
Cuts at: 650 2123 650
Size: 1473 4034
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4178 4362 4178
Size: 184 5323
MscI TGG'CCA
Cuts at: 3268 3268
Size: 5507
MslI CAynn'nnrTG
Cuts at: 3496 3623 3496
Size: 127 5380
MspA1I CmG'CkG
Cuts at: 2618 2932 3292 4305 4550 2618
Size: 314 360 1013 245 3575
Fragments arranged by size:
3575 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3688 3688
Size: 5507
NarI GG'CG_CC
Cuts at: 2938 3186 2938
Size: 248 5259
NcoI C'CATG_G
Cuts at: 107 1625 107
Size: 1518 3989
NgoMIV G'CCGG_C
Cuts at: 3686 3686
Size: 5507
NheI G'CTAG_C
Cuts at: 372 372
Size: 5507
NspI r_CATG'y
Cuts at: 123 2560 3060 3967 123
Size: 2437 500 907 1663
Fragments arranged by size:
2437 1663 907 500
PciI A'CATG_T
Cuts at: 119 3963 119
Size: 3844 1663
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 5507
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1959 1991 4646 4678 1959
Size: 32 2655 32 2788
Fragments arranged by size:
2788 2655 32 32
PpuMI rG'GwC_Cy
Cuts at: 1817 1817
Size: 5507
PshAI GACnn'nnGTC
Cuts at: 1833 1833
Size: 5507
PsiI TTA'TAA
Cuts at: 581 1609 581
Size: 1028 4479
PstI C_TGCA'G
Cuts at: 984 2554 3239 984
Size: 1570 685 3252
Fragments arranged by size:
3252 1570 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 2932 3292 2932
Size: 360 5147
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 1319 2521 1319
Size: 1202 4305
SalI G'TCGA_C
Cuts at: 2544 2544
Size: 5507
SapI GCTCTTCn'nnn_
Cuts at: 518 2123 3530 3740 518
Size: 1605 1407 210 2285
Fragments arranged by size:
2285 1605 1407 210
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 5024 5024
Size: 5507
SfcI C'TryA_G
Cuts at: 33 980 1929 1989 2550 3235 4228 4419 33
Size: 947 949 60 561 685 993 191 1121
Fragments arranged by size:
1121 993 949 947 685 561 191 60
SfoI GGC'GCC
Cuts at: 2939 3187 2939
Size: 248 5259
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 2529 2529
Size: 5507
SmlI C'TyrA_G
Cuts at: 271 538 957 2662 3577 4069 4331 271
Size: 267 419 1705 915 492 262 1447
Fragments arranged by size:
1705 1447 915 492 419 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5507
SphI G_CATG'C
Cuts at: 2560 3060 2560
Size: 500 5007
StuI AGG'CCT
Cuts at: 986 986
Size: 5507
StyI C'CwwG_G
Cuts at: 107 917 1625 1678 2641 2995 107
Size: 810 708 53 963 354 2619
Fragments arranged by size:
2619 963 810 708 354 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 1011 1495 1782 3090 3223 3682 3891 253
Size: 758 484 287 1308 133 459 209 1869
Fragments arranged by size:
1869 1308 758 484 459 287 209 133
TatI w'GTAC_w
Cuts at: 311 2351 5022 311
Size: 2040 2671 796
Fragments arranged by size:
2671 2040 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 990 1022 1301 1333 5024 5056 990
Size: 32 279 32 3691 32 1441
Fragments arranged by size:
3691 1441 279 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3304 3304
Size: 5507
XbaI T'CTAG_A
Cuts at: 1327 2538 1327
Size: 1211 4296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3002 3002
Size: 5507
XmaI C'CCGG_G
Cuts at: 2527 2527
Size: 5507
ZraI GAC'GTC
Cuts at: 721 721
Size: 5507
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI Bpu10I BsiWI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NdeI NotI NruI NsiI PacI
PasI PfoI PmlI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI