Restriction map for
pF1KB6284
(Circular) (Six-base) MAPSORT of: pF1KB6284.seq Check: 2897 from: 1 to: 5462
pF1KB6284 5462 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2500 2500
Size: 5462
Acc65I G'GTAC_C
Cuts at: 848 1475 2478 848
Size: 627 1003 3832
Fragments arranged by size:
3832 1003 627
AclI AA'CG_TT
Cuts at: 5302 5302
Size: 5462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 787 3287 3719 4466 4734 691
Size: 96 2500 432 747 268 1419
Fragments arranged by size:
2500 1419 747 432 268 96
AflIII A'CryG_T
Cuts at: 2814 3918 4895 2814
Size: 1104 977 3381
Fragments arranged by size:
3381 1104 977
AgeI A'CCGG_T
Cuts at: 3850 3850
Size: 5462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 152 1358 1409 152
Size: 1206 51 4205
Fragments arranged by size:
4205 1206 51
AlwNI CAG_nnn'CTG
Cuts at: 570 916 1083 1320 1978 2003 2310 2400 2767
Size: 346 167 237 658 25 307 90 367
Cuts at: 2767 4334 570
Size: 1567 1698
Fragments arranged by size:
1698 1567 658 367 346 307 237 167
90 25
ApaI G_GGCC'C
Cuts at: 453 1189 1261 1517 1962 453
Size: 736 72 256 445 3953
Fragments arranged by size:
3953 736 445 256 72
ApaLI G'TGCA_C
Cuts at: 2128 4232 2128
Size: 2104 3358
ApoI r'AATT_y
Cuts at: 2268 2466 4815 5071 2268
Size: 198 2349 256 2659
Fragments arranged by size:
2659 2349 256 198
AseI AT'TA_AT
Cuts at: 20 352 20
Size: 332 5130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
AvaI C'yCGr_G
Cuts at: 872 1295 2431 2482 872
Size: 423 1136 51 3852
Fragments arranged by size:
3852 1136 423 51
BamHI G'GATC_C
Cuts at: 707 795 1653 2487 707
Size: 88 858 834 3682
Fragments arranged by size:
3682 858 834 88
BanI G'GyrC_C
Cuts at: 727 770 848 1475 1716 1769 2478 2892 3140
Size: 43 78 627 241 53 709 414 248
Cuts at: 3140 3175 727
Size: 35 3014
Fragments arranged by size:
3014 709 627 414 248 241 78 53
43 35
BanII G_rGCy'C
Cuts at: 453 680 1189 1261 1302 1332 1517 1962 2096
Size: 227 509 72 41 30 185 445 134
Cuts at: 2096 2476 3506 453
Size: 380 1030 2409
Fragments arranged by size:
2409 1030 509 445 380 227 185 134
72 41 30
BbeI G_GCGC'C
Cuts at: 2896 3144 2896
Size: 248 5214
BbsI GAAGACnn'nnnn_
Cuts at: 1125 1125
Size: 5462
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1907 1941 1907
Size: 34 5428
BciVI GTATCCnnnnn_n'
Cuts at: 3360 4121 5462 3360
Size: 761 1341 3360
Fragments arranged by size:
3360 1341 761
BclI T'GATC_A
Cuts at: 740 1890 2982 740
Size: 1150 1092 3220
Fragments arranged by size:
3220 1150 1092
BglI GCCn_nnn'nGGC
Cuts at: 573 573
Size: 5462
BglII A'GATC_T
Cuts at: 2977 5014 2977
Size: 2037 3425
BlpI GC'TnA_GC
Cuts at: 551 909 2574 551
Size: 358 1665 3439
Fragments arranged by size:
3439 1665 358
Bme1580I G_kGCm'C
Cuts at: 453 730 1189 1261 1409 1517 1721 1954 1962
Size: 277 459 72 148 108 204 233 8
Cuts at: 1962 2132 3087 3180 4236 453
Size: 170 955 93 1056 1679
Fragments arranged by size:
1679 1056 955 459 277 233 204 170
148 108 93 72 8
BmrI ACTGGGnnnn_n'
Cuts at: 1071 1396 1830 1978 2003 2159 2674 2755 3090
Size: 325 434 148 25 156 515 81 335
Cuts at: 3090 5234 1071
Size: 2144 1299
Fragments arranged by size:
2144 1299 515 434 335 325 156 148
81 25
BmtI G_CTAG'C
Cuts at: 479 479
Size: 5462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 960 3829 960
Size: 2869 2593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 424 955 1286 1466 1488 1980 2201 320
Size: 104 531 331 180 22 492 221 3581
Fragments arranged by size:
3581 531 492 331 221 180 104 22
Bpu10I CC'TnA_GC
Cuts at: 1509 1609 1967 1509
Size: 100 358 5004
Fragments arranged by size:
5004 358 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2638 3517 4009 4307 2638
Size: 879 492 298 3793
Fragments arranged by size:
3793 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5166 5166
Size: 5462
BsaAI yAC'GTr
Cuts at: 2815 2815
Size: 5462
BsaBI GATnn'nnATC
Cuts at: 6 2976 5013 6
Size: 2970 2037 455
Fragments arranged by size:
2970 2037 455
BsaHI Gr'CG_yC
Cuts at: 1991 2893 3141 3843 5339 1991
Size: 902 248 702 1496 2114
Fragments arranged by size:
2114 1496 902 702 248
BsaWI w'CCGG_w
Cuts at: 767 1825 2651 3172 3850 4124 4271 4682 767
Size: 1058 826 521 678 274 147 411 1547
Fragments arranged by size:
1547 1058 826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 619
Size: 30 5432
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 518 952 2288 2437 2449 518
Size: 434 1336 149 12 3531
Fragments arranged by size:
3531 1336 434 149 12
BseYI C'CCAG_C
Cuts at: 570 1331 1352 1658 1868 2133 2408 2887 3645
Size: 761 21 306 210 265 275 479 758
Cuts at: 3645 4222 4718 570
Size: 577 496 1314
Fragments arranged by size:
1314 761 758 577 496 479 306 275
265 210 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 1271 1536 1220
Size: 51 265 5146
Fragments arranged by size:
5146 265 51
BsiEI CG_ry'CG
Cuts at: 104 3004 3050 4258 104
Size: 2900 46 1208 1308
Fragments arranged by size:
2900 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2132 2476 3254 3444 4236 2132
Size: 344 778 190 792 3358
Fragments arranged by size:
3358 792 778 344 190
Bsp1286I G_dGCh'C
Cuts at: 453 680 730 1189 1261 1302 1332 1409 1517
Size: 227 50 459 72 41 30 77 108
Cuts at: 1517 1721 1954 1962 2096 2132 2476 3087 3180
Size: 204 233 8 134 36 344 611 93
Cuts at: 3180 3254 3444 3506 4236 453
Size: 74 190 62 730 1679
Fragments arranged by size:
1679 730 611 459 344 233 227 204
190 134 108 93 77 74 72 62
50 41 36 30 8
BspEI T'CCGG_A
Cuts at: 4682 4682
Size: 5462
BspHI T'CATG_A
Cuts at: 4638 4638
Size: 5462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2512 2726 3028 3409 2512
Size: 214 302 381 4565
Fragments arranged by size:
4565 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3754 3808 5292 5457 50
Size: 3704 54 1484 165 55
Fragments arranged by size:
3704 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 387 3374 387
Size: 2987 2475
BsrFI r'CCGG_y
Cuts at: 1766 3460 3641 3850 1766
Size: 1694 181 209 3378
Fragments arranged by size:
3378 1694 209 181
BsrGI T'GTAC_A
Cuts at: 635 635
Size: 5462
BssSI C'ACGA_G
Cuts at: 3733 4091 3733
Size: 358 5104
BstAPI GCAn_nnn'nTGC
Cuts at: 770 2375 2839 770
Size: 1605 464 3393
Fragments arranged by size:
3393 1605 464
BstBI TT'CG_AA
Cuts at: 3823 3823
Size: 5462
BstXI CCAn_nnnn'nTGG
Cuts at: 933 1801 933
Size: 868 4594
BstYI r'GATC_y
Cuts at: 1 707 795 1441 1653 1906 2487 2954 2977
Size: 706 88 646 212 253 581 467 23
Cuts at: 2977 3312 3558 4570 4656 4668 5014 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 706 646 581 467 449 346 335
253 246 212 88 86 23 12
BtgI C'CryG_G
Cuts at: 1578 1578
Size: 5462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3412 3593 3756 3412
Size: 181 163 5118
Fragments arranged by size:
5118 181 163
BtsI GCAGTG_nn'
Cuts at: 1208 1443 1476 1620 2388 2705 1208
Size: 235 33 144 768 317 3965
Fragments arranged by size:
3965 768 317 235 144 33
DraI TTT'AAA
Cuts at: 2461 2461
Size: 5462
DrdI GACnn_nn'nnGTC
Cuts at: 1543 3168 4026 1543
Size: 1625 858 2979
Fragments arranged by size:
2979 1625 858
EaeI y'GGCC_r
Cuts at: 117 583 688 836 883 1146 3047 3221 3612
Size: 466 105 148 47 263 1901 174 391
Cuts at: 3612 3639 4984 117
Size: 27 1345 595
Fragments arranged by size:
1901 1345 595 466 391 263 174 148
105 47 27
EagI C'GGCC_G
Cuts at: 3047 3047
Size: 5462
EarI CTCTTCn'nnn_
Cuts at: 1533 3485 3695 1533
Size: 1952 210 3300
Fragments arranged by size:
3300 1952 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3670 3980 4126 5272 3670
Size: 310 146 1146 3860
Fragments arranged by size:
3860 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2474 2474
Size: 5462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 424 691 787 955 1286 1466 1488 1980
Size: 104 267 96 168 331 180 22 492
Cuts at: 1980 2201 3287 3719 4466 4734 320
Size: 221 1086 432 747 268 1048
Fragments arranged by size:
1086 1048 747 492 432 331 268 267
221 180 168 104 96 22
EcoO109I rG'GnC_Cy
Cuts at: 450 1087 1186 1258 1424 1513 1514 1958 1959
Size: 637 99 72 166 89 1 444 1
Cuts at: 1959 2083 2254 2335 2601 450
Size: 124 171 81 266 3311
Fragments arranged by size:
3311 637 444 266 171 166 124 99
89 81 72 1 1
EcoRI G'AATT_C
Cuts at: 2466 2466
Size: 5462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3481 3481
Size: 5462
FspI TGC'GCA
Cuts at: 763 3243 763
Size: 2480 2982
HaeII r_GCGC'y
Cuts at: 1320 2896 3144 4166 5145 1320
Size: 1576 248 1022 979 1637
Fragments arranged by size:
1637 1576 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1112 1144 1492 1524 3304 3334 3336 3366 1112
Size: 32 348 32 1780 30 2 30 3208
Fragments arranged by size:
3208 1780 348 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1548 1989 2501 1548
Size: 441 512 4509
Fragments arranged by size:
4509 512 441
KasI G'GCGC_C
Cuts at: 2892 3140 2892
Size: 248 5214
KpnI G_GTAC'C
Cuts at: 852 1479 2482 852
Size: 627 1003 3832
Fragments arranged by size:
3832 1003 627
MluI A'CGCG_T
Cuts at: 4895 4895
Size: 5462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 413 1934 4133 4317 413
Size: 1521 2199 184 1558
Fragments arranged by size:
2199 1558 1521 184
MscI TGG'CCA
Cuts at: 119 585 690 838 885 3223 119
Size: 466 105 148 47 2338 2358
Fragments arranged by size:
2358 2338 466 148 105 47
MslI CAynn'nnrTG
Cuts at: 736 1340 3451 3578 736
Size: 604 2111 127 2620
Fragments arranged by size:
2620 2111 604 127
MspA1I CmG'CkG
Cuts at: 126 132 211 369 558 570 834 860 902
Size: 6 79 158 189 12 264 26 42
Cuts at: 902 2573 2887 3247 4260 4505 126
Size: 1671 314 360 1013 245 1083
Fragments arranged by size:
1671 1083 1013 360 314 264 245 189
158 79 42 26 12 6
NaeI GCC'GGC
Cuts at: 3643 3643
Size: 5462
NarI GG'CG_CC
Cuts at: 2893 3141 2893
Size: 248 5214
NcoI C'CATG_G
Cuts at: 1578 1578
Size: 5462
NgoMIV G'CCGG_C
Cuts at: 3641 3641
Size: 5462
NheI G'CTAG_C
Cuts at: 475 475
Size: 5462
NspI r_CATG'y
Cuts at: 1047 1674 2515 3015 3922 1047
Size: 627 841 500 907 2587
Fragments arranged by size:
2587 907 841 627 500
PasI CC'CwG_GG
Cuts at: 1083 1955 1083
Size: 872 4590
PciI A'CATG_T
Cuts at: 3918 3918
Size: 5462
PflMI CCAn_nnn'nTGG
Cuts at: 1083 1222 1665 1978 2310 1083
Size: 139 443 313 332 4235
Fragments arranged by size:
4235 443 332 313 139
PfoI T'CCnGG_A
Cuts at: 1192 1302 1192
Size: 110 5352
PmeI GTTT'AAAC
Cuts at: 2461 2461
Size: 5462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 4601
Size: 32 5430
PpuMI rG'GwC_Cy
Cuts at: 1087 2083 1087
Size: 996 4466
PspOMI G'GGCC_C
Cuts at: 449 1185 1257 1513 1958 449
Size: 736 72 256 445 3953
Fragments arranged by size:
3953 736 445 256 72
PspXI vC'TCGA_Gb
Cuts at: 2431 2431
Size: 5462
PstI C_TGCA'G
Cuts at: 131 173 563 865 907 1603 1642 2509 3194
Size: 42 390 302 42 696 39 867 685
Cuts at: 3194 131
Size: 2399
Fragments arranged by size:
2399 867 696 685 390 302 42 42
39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5462
PvuII CAG'CTG
Cuts at: 126 132 558 834 860 902 2887 3247 126
Size: 6 426 276 26 42 1985 360 2341
Fragments arranged by size:
2341 1985 426 360 276 42 26 6
RsrII CG'GwC_CG
Cuts at: 3657 3657
Size: 5462
SacI G_AGCT'C
Cuts at: 2476 2476
Size: 5462
SalI G'TCGA_C
Cuts at: 2499 2499
Size: 5462
SanDI GG'GwC_CC
Cuts at: 1087 2083 1087
Size: 996 4466
SapI GCTCTTCn'nnn_
Cuts at: 1533 3485 3695 1533
Size: 1952 210 3300
Fragments arranged by size:
3300 1952 210
SbfI CC_TGCA'GG
Cuts at: 173 2509 173
Size: 2336 3126
ScaI AGT'ACT
Cuts at: 4979 4979
Size: 5462
SexAI A'CCwGG_T
Cuts at: 1110 1221 1110
Size: 111 5351
SfcI C'TryA_G
Cuts at: 33 127 169 559 861 903 1567 1599 1638
Size: 94 42 390 302 42 664 32 39
Cuts at: 1638 2505 3190 4183 4374 33
Size: 867 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 664 390 302 191
94 42 42 39 32
SfoI GGC'GCC
Cuts at: 2894 3142 2894
Size: 248 5214
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
SmaI CCC'GGG
Cuts at: 874 1297 2484 874
Size: 423 1187 3852
Fragments arranged by size:
3852 1187 423
SmlI C'TyrA_G
Cuts at: 2431 2617 3532 4024 4286 2431
Size: 186 915 492 262 3607
Fragments arranged by size:
3607 915 492 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5462
SphI G_CATG'C
Cuts at: 2515 3015 2515
Size: 500 4962
StuI AGG'CCT
Cuts at: 1845 2222 1845
Size: 377 5085
StyI C'CwwG_G
Cuts at: 1578 1703 2179 2596 2950 1578
Size: 125 476 417 354 4090
Fragments arranged by size:
4090 476 417 354 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2075 2147 3045 3178 3637 3846 2075
Size: 72 898 133 459 209 3691
Fragments arranged by size:
3691 898 459 209 133 72
TatI w'GTAC_w
Cuts at: 635 1634 4977 635
Size: 999 3343 1120
Fragments arranged by size:
3343 1120 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 480 512 1357 1389 4979 5011 480
Size: 32 845 32 3590 32 931
Fragments arranged by size:
3590 931 845 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1223 3259 1223
Size: 2036 3426
XbaI T'CTAG_A
Cuts at: 2493 2493
Size: 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 831 1539 2957 831
Size: 708 1418 3336
Fragments arranged by size:
3336 1418 708
XhoI C'TCGA_G
Cuts at: 2431 2431
Size: 5462
XmaI C'CCGG_G
Cuts at: 872 1295 2482 872
Size: 423 1187 3852
Fragments arranged by size:
3852 1187 423
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PmlI PshAI PsiI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI