Restriction map for
pF1KB6285
(Circular) (Six-base) MAPSORT of: pF1KB6285.seq Check: 3439 from: 1 to: 5699
pF1KB6285 5699 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 2737 2737
Size: 5699
Acc65I G'GTAC_C
Cuts at: 2715 2715
Size: 5699
AclI AA'CG_TT
Cuts at: 5539 5539
Size: 5699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1384 1514 1697 2710 3524 3956 4703 4971
Size: 591 130 183 1013 814 432 747 268
Cuts at: 4971 793
Size: 1521
Fragments arranged by size:
1521 1013 814 747 591 432 268 183
130
AflII C'TTAA_G
Cuts at: 1075 2353 1075
Size: 1278 4421
AflIII A'CryG_T
Cuts at: 3051 4155 5132 3051
Size: 1104 977 3618
Fragments arranged by size:
3618 1104 977
AgeI A'CCGG_T
Cuts at: 4087 4087
Size: 5699
AleI CACnn'nnGTG
Cuts at: 223 223
Size: 5699
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 2143
Size: 32 5667
AlwNI CAG_nnn'CTG
Cuts at: 263 291 844 3004 4571 263
Size: 28 553 2160 1567 1391
Fragments arranged by size:
2160 1567 1391 553 28
ApaI G_GGCC'C
Cuts at: 1357 1645 1357
Size: 288 5411
ApaLI G'TGCA_C
Cuts at: 4469 4469
Size: 5699
ApoI r'AATT_y
Cuts at: 1012 1871 2096 2287 2703 5052 5308 1012
Size: 859 225 191 416 2349 256 1403
Fragments arranged by size:
2349 1403 859 416 256 225 191
AseI AT'TA_AT
Cuts at: 20 613 854 20
Size: 593 241 4865
Fragments arranged by size:
4865 593 241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
AvaI C'yCGr_G
Cuts at: 165 2666 2719 165
Size: 2501 53 3145
Fragments arranged by size:
3145 2501 53
AvrII C'CTAG_G
Cuts at: 364 364
Size: 5699
BamHI G'GATC_C
Cuts at: 2724 2724
Size: 5699
BanI G'GyrC_C
Cuts at: 136 235 2715 3129 3377 3412 136
Size: 99 2480 414 248 35 2423
Fragments arranged by size:
2480 2423 414 248 99 35
BanII G_rGCy'C
Cuts at: 275 818 1357 1645 2085 2673 2713 3743 275
Size: 543 539 288 440 588 40 1030 2231
Fragments arranged by size:
2231 1030 588 543 539 440 288 40
BbeI G_GCGC'C
Cuts at: 140 239 3133 3381 140
Size: 99 2894 248 2458
Fragments arranged by size:
2894 2458 248 99
BbsI GAAGACnn'nnnn_
Cuts at: 1037 1540 1792 1037
Size: 503 252 4944
Fragments arranged by size:
4944 503 252
BbvCI CC'TCA_GC
Cuts at: 500 1052 500
Size: 552 5147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5665
BciVI GTATCCnnnnn_n'
Cuts at: 3597 4358 5699 3597
Size: 761 1341 3597
Fragments arranged by size:
3597 1341 761
BclI T'GATC_A
Cuts at: 3219 3219
Size: 5699
BglII A'GATC_T
Cuts at: 3214 5251 3214
Size: 2037 3662
BlpI GC'TnA_GC
Cuts at: 2216 2811 2216
Size: 595 5104
Bme1580I G_kGCm'C
Cuts at: 1357 1645 3324 3417 4473 1357
Size: 288 1679 93 1056 2583
Fragments arranged by size:
2583 1679 1056 288 93
BmrI ACTGGGnnnn_n'
Cuts at: 2911 2992 3327 5471 2911
Size: 81 335 2144 3139
Fragments arranged by size:
3139 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4066 4066
Size: 5699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1277 1349 1598 1814 431
Size: 846 72 249 216 4316
Fragments arranged by size:
4316 846 249 216 72
Bpu10I CC'TnA_GC
Cuts at: 500 1052 500
Size: 552 5147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 1024 1209 2875 3754 4246 4544 528
Size: 496 185 1666 879 492 298 1683
Fragments arranged by size:
1683 1666 879 496 492 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 517 1455 2499 5403 517
Size: 938 1044 2904 813
Fragments arranged by size:
2904 1044 938 813
BsaAI yAC'GTr
Cuts at: 3052 3052
Size: 5699
BsaBI GATnn'nnATC
Cuts at: 6 3213 5250 6
Size: 3207 2037 455
Fragments arranged by size:
3207 2037 455
BsaHI Gr'CG_yC
Cuts at: 137 236 280 3130 3378 4080 5576 137
Size: 99 44 2850 248 702 1496 260
Fragments arranged by size:
2850 1496 702 260 248 99 44
BsaWI w'CCGG_w
Cuts at: 222 330 2888 3409 4087 4361 4508 4919 222
Size: 108 2558 521 678 274 147 411 1002
Fragments arranged by size:
2558 1002 678 521 411 274 147 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 234 264 723 753 1182 1212 2143 2173 2644
Size: 30 459 30 429 30 931 30 471
Cuts at: 2644 2674 234
Size: 30 3259
Fragments arranged by size:
3259 931 471 459 429 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 368 488 368
Size: 120 5579
BseYI C'CCAG_C
Cuts at: 284 1579 1634 1663 3124 3882 4459 4955 284
Size: 1295 55 29 1461 758 577 496 1028
Fragments arranged by size:
1461 1295 1028 758 577 496 55 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1556
Size: 5699
BsiEI CG_ry'CG
Cuts at: 104 191 3241 3287 4495 104
Size: 87 3050 46 1208 1308
Fragments arranged by size:
3050 1308 1208 87 46
BsiHKAI G_wGCw'C
Cuts at: 220 2085 2673 2713 3491 3681 4473 220
Size: 1865 588 40 778 190 792 1446
Fragments arranged by size:
1865 1446 792 778 588 190 40
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 2453
Size: 5699
Bsp1286I G_dGCh'C
Cuts at: 220 275 818 1357 1645 2085 2673 2713 3324
Size: 55 543 539 288 440 588 40 611
Cuts at: 3324 3417 3491 3681 3743 4473 220
Size: 93 74 190 62 730 1446
Fragments arranged by size:
1446 730 611 588 543 539 440 288
190 93 74 62 55 40
BspEI T'CCGG_A
Cuts at: 4919 4919
Size: 5699
BspHI T'CATG_A
Cuts at: 4875 4875
Size: 5699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2749 2963 3265 3646 2749
Size: 214 302 381 4802
Fragments arranged by size:
4802 381 302 214
BsrBI CCG'CTC
Cuts at: 50 142 3991 4045 5529 5694 50
Size: 92 3849 54 1484 165 55
Fragments arranged by size:
3849 1484 165 92 55 54
BsrDI GCAATG_nn'
Cuts at: 914 1141 1166 1728 3611 914
Size: 227 25 562 1883 3002
Fragments arranged by size:
3002 1883 562 227 25
BsrFI r'CCGG_y
Cuts at: 115 133 201 3697 3878 4087 115
Size: 18 68 3496 181 209 1727
Fragments arranged by size:
3496 1727 209 181 68 18
BsrGI T'GTAC_A
Cuts at: 586 586
Size: 5699
BssSI C'ACGA_G
Cuts at: 2604 3970 4328 2604
Size: 1366 358 3975
Fragments arranged by size:
3975 1366 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3076 3076
Size: 5699
BstBI TT'CG_AA
Cuts at: 4060 4060
Size: 5699
BstEII G'GTnAC_C
Cuts at: 315 672 315
Size: 357 5342
BstYI r'GATC_y
Cuts at: 1 2385 2724 3191 3214 3549 3795 4807 4893
Size: 2384 339 467 23 335 246 1012 86
Cuts at: 4893 4905 5251 1
Size: 12 346 449
Fragments arranged by size:
2384 1012 467 449 346 339 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2617 2617
Size: 5699
BtgI C'CryG_G
Cuts at: 179 275 302 1445 179
Size: 96 27 1143 4433
Fragments arranged by size:
4433 1143 96 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3649 3830 3993 3649
Size: 181 163 5355
Fragments arranged by size:
5355 181 163
BtsI GCAGTG_nn'
Cuts at: 635 1453 2942 635
Size: 818 1489 3392
Fragments arranged by size:
3392 1489 818
DraI TTT'AAA
Cuts at: 2698 2698
Size: 5699
DrdI GACnn_nn'nnGTC
Cuts at: 3405 4263 3405
Size: 858 4841
EaeI y'GGCC_r
Cuts at: 188 3284 3458 3849 3876 5221 188
Size: 3096 174 391 27 1345 666
Fragments arranged by size:
3096 1345 666 391 174 27
EagI C'GGCC_G
Cuts at: 188 3284 188
Size: 3096 2603
EarI CTCTTCn'nnn_
Cuts at: 2497 3722 3932 2497
Size: 1225 210 4264
Fragments arranged by size:
4264 1225 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3907 4217 4363 5509 3907
Size: 310 146 1146 4097
Fragments arranged by size:
4097 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2083 2671 2711 2083
Size: 588 40 5071
Fragments arranged by size:
5071 588 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 793 1277 1349 1384 1514 1598 1697 1814
Size: 362 484 72 35 130 84 99 117
Cuts at: 1814 2710 3524 3956 4703 4971 431
Size: 896 814 432 747 268 1159
Fragments arranged by size:
1159 896 814 747 484 432 362 268
130 117 99 84 72 35
EcoNI CCTnn'n_nnAGG
Cuts at: 1715 2689 1715
Size: 974 4725
EcoO109I rG'GnC_Cy
Cuts at: 308 1642 2838 308
Size: 1334 1196 3169
Fragments arranged by size:
3169 1334 1196
EcoRI G'AATT_C
Cuts at: 2703 2703
Size: 5699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 3718 2560
Size: 1158 4541
FspI TGC'GCA
Cuts at: 3480 3480
Size: 5699
HaeII r_GCGC'y
Cuts at: 140 164 199 239 341 3133 3381 4403 5382
Size: 24 35 40 102 2792 248 1022 979
Cuts at: 5382 140
Size: 457
Fragments arranged by size:
2792 1022 979 457 248 102 40 35
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 234 266 2260 2292 3541 3571 3573 3603 234
Size: 32 1994 32 1249 30 2 30 2330
Fragments arranged by size:
2330 1994 1249 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1983 2738 1983
Size: 755 4944
KasI G'GCGC_C
Cuts at: 136 235 3129 3377 136
Size: 99 2894 248 2458
Fragments arranged by size:
2894 2458 248 99
KpnI G_GTAC'C
Cuts at: 2719 2719
Size: 5699
MfeI C'AATT_G
Cuts at: 2210 2308 2431 2210
Size: 98 123 5478
Fragments arranged by size:
5478 123 98
MluI A'CGCG_T
Cuts at: 5132 5132
Size: 5699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 889 4370 4554 889
Size: 3481 184 2034
Fragments arranged by size:
3481 2034 184
MscI TGG'CCA
Cuts at: 3460 3460
Size: 5699
MslI CAynn'nnrTG
Cuts at: 223 3688 3815 223
Size: 3465 127 2107
Fragments arranged by size:
3465 2107 127
MspA1I CmG'CkG
Cuts at: 359 844 1384 1663 2397 2810 3124 3484 4497
Size: 485 540 279 734 413 314 360 1013
Cuts at: 4497 4742 359
Size: 245 1316
Fragments arranged by size:
1316 1013 734 540 485 413 360 314
279 245
NaeI GCC'GGC
Cuts at: 117 135 3880 117
Size: 18 3745 1936
Fragments arranged by size:
3745 1936 18
NarI GG'CG_CC
Cuts at: 137 236 3130 3378 137
Size: 99 2894 248 2458
Fragments arranged by size:
2894 2458 248 99
NcoI C'CATG_G
Cuts at: 179 179
Size: 5699
NgoMIV G'CCGG_C
Cuts at: 115 133 3878 115
Size: 18 3745 1936
Fragments arranged by size:
3745 1936 18
NspI r_CATG'y
Cuts at: 929 2752 3252 4159 929
Size: 1823 500 907 2469
Fragments arranged by size:
2469 1823 907 500
PasI CC'CwG_GG
Cuts at: 231 231
Size: 5699
PciI A'CATG_T
Cuts at: 4155 4155
Size: 5699
PflMI CCAn_nnn'nTGG
Cuts at: 1306 1306
Size: 5699
PfoI T'CCnGG_A
Cuts at: 447 741 447
Size: 294 5405
PmeI GTTT'AAAC
Cuts at: 2698 2698
Size: 5699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 589 621 2143 2175 4838 4870 589
Size: 32 1522 32 2663 32 1418
Fragments arranged by size:
2663 1522 1418 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 308 308
Size: 5699
PshAI GACnn'nnGTC
Cuts at: 1447 1447
Size: 5699
PspOMI G'GGCC_C
Cuts at: 1353 1641 1353
Size: 288 5411
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 665 697 665
Size: 32 5667
PstI C_TGCA'G
Cuts at: 2746 3431 2746
Size: 685 5014
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5699
PvuII CAG'CTG
Cuts at: 844 1384 1663 2397 3124 3484 844
Size: 540 279 734 727 360 3059
Fragments arranged by size:
3059 734 727 540 360 279
RsrII CG'GwC_CG
Cuts at: 3894 3894
Size: 5699
SacI G_AGCT'C
Cuts at: 2085 2673 2713 2085
Size: 588 40 5071
Fragments arranged by size:
5071 588 40
SalI G'TCGA_C
Cuts at: 2736 2736
Size: 5699
SanDI GG'GwC_CC
Cuts at: 308 308
Size: 5699
SapI GCTCTTCn'nnn_
Cuts at: 3722 3932 3722
Size: 210 5489
SbfI CC_TGCA'GG
Cuts at: 2746 2746
Size: 5699
ScaI AGT'ACT
Cuts at: 5216 5216
Size: 5699
SexAI A'CCwGG_T
Cuts at: 1061 2272 1061
Size: 1211 4488
SfcI C'TryA_G
Cuts at: 33 1214 2742 3427 4420 4611 33
Size: 1181 1528 685 993 191 1121
Fragments arranged by size:
1528 1181 1121 993 685 191
SfoI GGC'GCC
Cuts at: 138 237 3131 3379 138
Size: 99 2894 248 2458
Fragments arranged by size:
2894 2458 248 99
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
SgrAI Cr'CCGG_yG
Cuts at: 133 133
Size: 5699
SmaI CCC'GGG
Cuts at: 2721 2721
Size: 5699
SmlI C'TyrA_G
Cuts at: 543 1039 1075 1224 2353 2666 2854 3769 4261
Size: 496 36 149 1129 313 188 915 492
Cuts at: 4261 4523 543
Size: 262 1719
Fragments arranged by size:
1719 1129 915 496 492 313 262 188
149 36
SpeI A'CTAG_T
Cuts at: 67 1695 1816 67
Size: 1628 121 3950
Fragments arranged by size:
3950 1628 121
SphI G_CATG'C
Cuts at: 2752 3252 2752
Size: 500 5199
StuI AGG'CCT
Cuts at: 2189 2189
Size: 5699
StyI C'CwwG_G
Cuts at: 179 364 2494 2833 3187 179
Size: 185 2130 339 354 2691
Fragments arranged by size:
2691 2130 354 339 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 3282 3415 3874 4083 1284
Size: 1998 133 459 209 2900
Fragments arranged by size:
2900 1998 459 209 133
TatI w'GTAC_w
Cuts at: 586 699 1177 5214 586
Size: 113 478 4037 1071
Fragments arranged by size:
4037 1071 478 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 653 685 723 755 5216 5248 653
Size: 32 38 32 4461 32 1104
Fragments arranged by size:
4461 1104 38 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1162 3496 1162
Size: 2334 3365
XbaI T'CTAG_A
Cuts at: 2193 2730 2193
Size: 537 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3194 3194
Size: 5699
XhoI C'TCGA_G
Cuts at: 2666 2666
Size: 5699
XmaI C'CCGG_G
Cuts at: 2719 2719
Size: 5699
XmnI GAAnn'nnTTC
Cuts at: 2066 2066
Size: 5699
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AscI BaeI BfrBI
BglI BmgBI BmtI BsiWI BsmI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI SacII SfiI SnaBI SrfI SspI SwaI ZraI