Restriction map for
pF1KB6289
(Circular) (Six-base) MAPSORT of: pF1KB6289.seq Check: 8467 from: 1 to: 5402
pF1KB6289 5402 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 1006 2204 1006
Size: 1198 4204
AccI GT'mk_AC
Cuts at: 2440 2440
Size: 5402
Acc65I G'GTAC_C
Cuts at: 1242 2418 1242
Size: 1176 4226
AclI AA'CG_TT
Cuts at: 5242 5242
Size: 5402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 3659 4406 4674 3227
Size: 432 747 268 3955
Fragments arranged by size:
3955 747 432 268
AflIII A'CryG_T
Cuts at: 986 2754 3858 4835 986
Size: 1768 1104 977 1553
Fragments arranged by size:
1768 1553 1104 977
AgeI A'CCGG_T
Cuts at: 3790 3790
Size: 5402
AhdI GACnn_n'nnGTC
Cuts at: 1095 1095
Size: 5402
AleI CACnn'nnGTG
Cuts at: 1043 1043
Size: 5402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 645 2326 645
Size: 1681 3721
AlwNI CAG_nnn'CTG
Cuts at: 1272 2707 4274 1272
Size: 1435 1567 2400
Fragments arranged by size:
2400 1567 1435
ApaI G_GGCC'C
Cuts at: 416 1805 2356 416
Size: 1389 551 3462
Fragments arranged by size:
3462 1389 551
ApaLI G'TGCA_C
Cuts at: 4172 4172
Size: 5402
ApoI r'AATT_y
Cuts at: 421 2406 4755 5011 421
Size: 1985 2349 256 812
Fragments arranged by size:
2349 1985 812 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5402
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
AvaI C'yCGr_G
Cuts at: 1111 1941 2422 1111
Size: 830 481 4091
Fragments arranged by size:
4091 830 481
BamHI G'GATC_C
Cuts at: 2427 2427
Size: 5402
BanI G'GyrC_C
Cuts at: 1242 1296 2041 2418 2832 3080 3115 1242
Size: 54 745 377 414 248 35 3529
Fragments arranged by size:
3529 745 414 377 248 54 35
BanII G_rGCy'C
Cuts at: 416 450 582 697 1154 1330 1552 1805 1832
Size: 34 132 115 457 176 222 253 27
Cuts at: 1832 2356 2416 3446 416
Size: 524 60 1030 2372
Fragments arranged by size:
2372 1030 524 457 253 222 176 132
115 60 34 27
BbeI G_GCGC'C
Cuts at: 2836 3084 2836
Size: 248 5154
BbvCI CC'TCA_GC
Cuts at: 1385 2299 1385
Size: 914 4488
BciVI GTATCCnnnnn_n'
Cuts at: 3300 4061 5402 3300
Size: 761 1341 3300
Fragments arranged by size:
3300 1341 761
BclI T'GATC_A
Cuts at: 2922 2922
Size: 5402
BglII A'GATC_T
Cuts at: 2917 4954 2917
Size: 2037 3365
BlpI GC'TnA_GC
Cuts at: 1870 2514 1870
Size: 644 4758
Bme1580I G_kGCm'C
Cuts at: 416 1805 2356 3027 3120 4176 416
Size: 1389 551 671 93 1056 1642
Fragments arranged by size:
1642 1389 1056 671 551 93
BmrI ACTGGGnnnn_n'
Cuts at: 700 1037 1799 2017 2359 2370 2614 2695 3030
Size: 337 762 218 342 11 244 81 335
Cuts at: 3030 5174 700
Size: 2144 928
Fragments arranged by size:
2144 928 762 342 337 335 244 218
81 11
BmtI G_CTAG'C
Cuts at: 1384 1384
Size: 5402
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 1587 1754 3769 285
Size: 1302 167 2015 1918
Fragments arranged by size:
2015 1918 1302 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 541 596 763 1998 446
Size: 95 55 167 1235 3850
Fragments arranged by size:
3850 1235 167 95 55
Bpu10I CC'TnA_GC
Cuts at: 1385 1666 2299 2387 1385
Size: 281 633 88 4400
Fragments arranged by size:
4400 633 281 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 3457 3949 4247 2578
Size: 879 492 298 3733
Fragments arranged by size:
3733 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 517 595 771 1026 1103 1524 1742 5106 517
Size: 78 176 255 77 421 218 3364 813
Fragments arranged by size:
3364 813 421 255 218 176 78 77
BsaAI yAC'GTr
Cuts at: 899 2755 899
Size: 1856 3546
BsaBI GATnn'nnATC
Cuts at: 6 557 2916 4953 6
Size: 551 2359 2037 455
Fragments arranged by size:
2359 2037 551 455
BsaHI Gr'CG_yC
Cuts at: 1003 2201 2833 3081 3783 5279 1003
Size: 1198 632 248 702 1496 1126
Fragments arranged by size:
1496 1198 1126 702 632 248
BsaWI w'CCGG_w
Cuts at: 2591 3112 3790 4064 4211 4622 2591
Size: 521 678 274 147 411 3371
Fragments arranged by size:
3371 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 444 474 1606 1636 1846 1876 2146 2176 444
Size: 30 1132 30 210 30 270 30 3670
Fragments arranged by size:
3670 1132 270 210 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 781 1391 1402 1444 1734 1743 1839 1923
Size: 573 610 11 42 290 9 96 84
Cuts at: 1923 2125 208
Size: 202 3485
Fragments arranged by size:
3485 610 573 290 202 96 84 42
11 9
BseYI C'CCAG_C
Cuts at: 286 1817 1839 1865 1992 2827 3585 4162 4658
Size: 1531 22 26 127 835 758 577 496
Cuts at: 4658 286
Size: 1030
Fragments arranged by size:
1531 1030 835 758 577 496 127 26
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 629 964 615
Size: 14 335 5053
Fragments arranged by size:
5053 335 14
BsiEI CG_ry'CG
Cuts at: 104 1141 2944 2990 4198 104
Size: 1037 1803 46 1208 1308
Fragments arranged by size:
1803 1308 1208 1037 46
BsiHKAI G_wGCw'C
Cuts at: 1118 1154 1330 1832 2109 2416 3194 3384 4176
Size: 36 176 502 277 307 778 190 792
Cuts at: 4176 1118
Size: 2344
Fragments arranged by size:
2344 792 778 502 307 277 190 176
36
BsmBI CGTCTCn'nnnn_
Cuts at: 994 994
Size: 5402
Bsp1286I G_dGCh'C
Cuts at: 416 450 582 697 1118 1154 1330 1552 1805
Size: 34 132 115 421 36 176 222 253
Cuts at: 1805 1832 2109 2356 2416 3027 3120 3194 3384
Size: 27 277 247 60 611 93 74 190
Cuts at: 3384 3446 4176 416
Size: 62 730 1642
Fragments arranged by size:
1642 730 611 421 277 253 247 222
190 176 132 115 93 74 62 60
36 34 27
BspEI T'CCGG_A
Cuts at: 4622 4622
Size: 5402
BspHI T'CATG_A
Cuts at: 1322 4578 1322
Size: 3256 2146
BspMI ACCTGCnnnn'nnnn_
Cuts at: 549 2452 2666 2968 3349 549
Size: 1903 214 302 381 2602
Fragments arranged by size:
2602 1903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 813 3694 3748 5232 5397 50
Size: 763 2881 54 1484 165 55
Fragments arranged by size:
2881 1484 763 165 55 54
BsrDI GCAATG_nn'
Cuts at: 932 1300 1361 3314 932
Size: 368 61 1953 3020
Fragments arranged by size:
3020 1953 368 61
BsrFI r'CCGG_y
Cuts at: 1733 3400 3581 3790 1733
Size: 1667 181 209 3345
Fragments arranged by size:
3345 1667 209 181
BssSI C'ACGA_G
Cuts at: 3673 4031 3673
Size: 358 5044
BstAPI GCAn_nnn'nTGC
Cuts at: 382 1758 2779 382
Size: 1376 1021 3005
Fragments arranged by size:
3005 1376 1021
BstBI TT'CG_AA
Cuts at: 669 3763 669
Size: 3094 2308
BstXI CCAn_nnnn'nTGG
Cuts at: 255 1542 255
Size: 1287 4115
BstYI r'GATC_y
Cuts at: 1 208 2079 2306 2427 2894 2917 3252 3498
Size: 207 1871 227 121 467 23 335 246
Cuts at: 3498 4510 4596 4608 4954 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1871 1012 467 449 346 335 246 227
207 121 86 23 12
BtgI C'CryG_G
Cuts at: 1222 1222
Size: 5402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3352 3533 3696 3352
Size: 181 163 5058
Fragments arranged by size:
5058 181 163
BtsI GCAGTG_nn'
Cuts at: 1213 2645 1213
Size: 1432 3970
DraI TTT'AAA
Cuts at: 2401 2401
Size: 5402
DrdI GACnn_nn'nnGTC
Cuts at: 991 3108 3966 991
Size: 2117 858 2427
Fragments arranged by size:
2427 2117 858
EaeI y'GGCC_r
Cuts at: 127 335 814 833 2987 3161 3552 3579 4924
Size: 208 479 19 2154 174 391 27 1345
Cuts at: 4924 127
Size: 605
Fragments arranged by size:
2154 1345 605 479 391 208 174 27
19
EagI C'GGCC_G
Cuts at: 2987 2987
Size: 5402
EarI CTCTTCn'nnn_
Cuts at: 1556 1795 2187 3425 3635 1556
Size: 239 392 1238 210 3323
Fragments arranged by size:
3323 1238 392 239 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 952 1144 1215 3610 3920 4066 5212 952
Size: 192 71 2395 310 146 1146 1142
Fragments arranged by size:
2395 1146 1142 310 192 146 71
EcoICRI GAG'CTC
Cuts at: 1152 1328 1830 2414 1152
Size: 176 502 584 4140
Fragments arranged by size:
4140 584 502 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 541 596 763 1998 3227 3659 4406 4674
Size: 95 55 167 1235 1229 432 747 268
Cuts at: 4674 446
Size: 1174
Fragments arranged by size:
1235 1229 1174 747 432 268 167 95
55
EcoNI CCTnn'n_nnAGG
Cuts at: 2338 2338
Size: 5402
EcoO109I rG'GnC_Cy
Cuts at: 1670 1801 1894 2343 2353 2541 1670
Size: 131 93 449 10 188 4531
Fragments arranged by size:
4531 449 188 131 93 10
EcoRI G'AATT_C
Cuts at: 2406 2406
Size: 5402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1041 3421 1041
Size: 2380 3022
FspI TGC'GCA
Cuts at: 3183 3183
Size: 5402
HaeII r_GCGC'y
Cuts at: 2836 3084 4106 5085 2836
Size: 248 1022 979 3153
Fragments arranged by size:
3153 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1081 1113 1386 1418 1585 1606 1617 1638 1846
Size: 32 273 32 167 21 11 21 208
Cuts at: 1846 1878 2146 2178 3244 3274 3276 3306 1081
Size: 32 268 32 1066 30 2 30 3177
Fragments arranged by size:
3177 1066 273 268 208 167 32 32
32 32 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 2441 2441
Size: 5402
KasI G'GCGC_C
Cuts at: 2832 3080 2832
Size: 248 5154
KpnI G_GTAC'C
Cuts at: 1246 2422 1246
Size: 1176 4226
MfeI C'AATT_G
Cuts at: 1105 1105
Size: 5402
MluI A'CGCG_T
Cuts at: 4835 4835
Size: 5402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 677 4073 4257 290
Size: 387 3396 184 1435
Fragments arranged by size:
3396 1435 387 184
MscI TGG'CCA
Cuts at: 337 835 3163 337
Size: 498 2328 2576
Fragments arranged by size:
2576 2328 498
MslI CAynn'nnrTG
Cuts at: 835 1043 1540 3391 3518 835
Size: 208 497 1851 127 2719
Fragments arranged by size:
2719 1851 497 208 127
MspA1I CmG'CkG
Cuts at: 286 437 1817 2513 2827 3187 4200 4445 286
Size: 151 1380 696 314 360 1013 245 1243
Fragments arranged by size:
1380 1243 1013 696 360 314 245 151
NaeI GCC'GGC
Cuts at: 3583 3583
Size: 5402
NarI GG'CG_CC
Cuts at: 2833 3081 2833
Size: 248 5154
NgoMIV G'CCGG_C
Cuts at: 3581 3581
Size: 5402
NheI G'CTAG_C
Cuts at: 1380 1380
Size: 5402
NspI r_CATG'y
Cuts at: 990 2455 2955 3862 990
Size: 1465 500 907 2530
Fragments arranged by size:
2530 1465 907 500
PasI CC'CwG_GG
Cuts at: 1093 1188 2037 2067 1093
Size: 95 849 30 4428
Fragments arranged by size:
4428 849 95 30
PciI A'CATG_T
Cuts at: 986 3858 986
Size: 2872 2530
PflMI CCAn_nnn'nTGG
Cuts at: 1272 1887 1272
Size: 615 4787
PfoI T'CCnGG_A
Cuts at: 462 995 1975 2276 462
Size: 533 980 301 3588
Fragments arranged by size:
3588 980 533 301
PmeI GTTT'AAAC
Cuts at: 2401 2401
Size: 5402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1819 1851 4541 4573 1819
Size: 32 2690 32 2648
Fragments arranged by size:
2690 2648 32 32
PpuMI rG'GwC_Cy
Cuts at: 1670 1894 2343 1670
Size: 224 449 4729
Fragments arranged by size:
4729 449 224
PspOMI G'GGCC_C
Cuts at: 412 1801 2352 412
Size: 1389 551 3462
Fragments arranged by size:
3462 1389 551
PstI C_TGCA'G
Cuts at: 546 1416 2449 3134 546
Size: 870 1033 685 2814
Fragments arranged by size:
2814 1033 870 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5402
PvuII CAG'CTG
Cuts at: 286 437 2827 3187 286
Size: 151 2390 360 2501
Fragments arranged by size:
2501 2390 360 151
RsrII CG'GwC_CG
Cuts at: 3597 3597
Size: 5402
SacI G_AGCT'C
Cuts at: 1154 1330 1832 2416 1154
Size: 176 502 584 4140
Fragments arranged by size:
4140 584 502 176
SalI G'TCGA_C
Cuts at: 2439 2439
Size: 5402
SapI GCTCTTCn'nnn_
Cuts at: 1795 3425 3635 1795
Size: 1630 210 3562
Fragments arranged by size:
3562 1630 210
SbfI CC_TGCA'GG
Cuts at: 2449 2449
Size: 5402
ScaI AGT'ACT
Cuts at: 1809 4919 1809
Size: 3110 2292
SexAI A'CCwGG_T
Cuts at: 1070 1238 1070
Size: 168 5234
SfcI C'TryA_G
Cuts at: 33 542 1412 1656 2392 2445 3130 4123 4314
Size: 509 870 244 736 53 685 993 191
Cuts at: 4314 33
Size: 1121
Fragments arranged by size:
1121 993 870 736 685 509 244 191
53
SfoI GGC'GCC
Cuts at: 2834 3082 2834
Size: 248 5154
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
SmaI CCC'GGG
Cuts at: 2424 2424
Size: 5402
SmlI C'TyrA_G
Cuts at: 2557 3472 3964 4226 2557
Size: 915 492 262 3733
Fragments arranged by size:
3733 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5402
SphI G_CATG'C
Cuts at: 2455 2955 2455
Size: 500 4902
SspI AAT'ATT
Cuts at: 357 357
Size: 5402
StuI AGG'CCT
Cuts at: 244 2270 244
Size: 2026 3376
StyI C'CwwG_G
Cuts at: 248 386 527 1370 1556 2536 2890 248
Size: 138 141 843 186 980 354 2760
Fragments arranged by size:
2760 980 843 354 186 141 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1923 2985 3118 3577 3786 1155
Size: 768 1062 133 459 209 2771
Fragments arranged by size:
2771 1062 768 459 209 133
TatI w'GTAC_w
Cuts at: 1807 2025 4917 1807
Size: 218 2892 2292
Fragments arranged by size:
2892 2292 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 958 990 4919 4951 958
Size: 32 3929 32 1409
Fragments arranged by size:
3929 1409 32 32
Tth111I GACn'n_nGTC
Cuts at: 3199 3199
Size: 5402
XbaI T'CTAG_A
Cuts at: 2433 2433
Size: 5402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 788 2897 788
Size: 2109 3293
XmaI C'CCGG_G
Cuts at: 2422 2422
Size: 5402
ZraI GAC'GTC
Cuts at: 1004 2202 1004
Size: 1198 4204
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI AscI AvrII BaeI BbsI
BcgI BfrBI BglI BmgBI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI