Restriction map for
pF1KB6301
(Circular) (Six-base) MAPSORT of: pF1KB6301.seq Check: 8252 from: 1 to: 3863
pF1KB6301 3863 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 336 336
Size: 3863
AccI GT'mk_AC
Cuts at: 341 901 341
Size: 560 3303
Acc65I G'GTAC_C
Cuts at: 879 879
Size: 3863
AclI AA'CG_TT
Cuts at: 3703 3703
Size: 3863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 2120 2867 3135 1688
Size: 432 747 268 2416
Fragments arranged by size:
2416 747 432 268
AflIII A'CryG_T
Cuts at: 1215 2319 3296 1215
Size: 1104 977 1782
Fragments arranged by size:
1782 1104 977
AgeI A'CCGG_T
Cuts at: 2251 2251
Size: 3863
AlwNI CAG_nnn'CTG
Cuts at: 1168 2735 1168
Size: 1567 2296
ApaLI G'TGCA_C
Cuts at: 2633 2633
Size: 3863
ApoI r'AATT_y
Cuts at: 812 867 3216 3472 812
Size: 55 2349 256 1203
Fragments arranged by size:
2349 1203 256 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
AvaI C'yCGr_G
Cuts at: 244 883 244
Size: 639 3224
BamHI G'GATC_C
Cuts at: 888 888
Size: 3863
BanI G'GyrC_C
Cuts at: 252 279 289 430 486 879 1293 1541 1576
Size: 27 10 141 56 393 414 248 35
Cuts at: 1576 252
Size: 2539
Fragments arranged by size:
2539 414 393 248 141 56 35 27
10
BanII G_rGCy'C
Cuts at: 877 1907 877
Size: 1030 2833
BbeI G_GCGC'C
Cuts at: 293 434 1297 1545 293
Size: 141 863 248 2611
Fragments arranged by size:
2611 863 248 141
BciVI GTATCCnnnnn_n'
Cuts at: 1761 2522 3863 1761
Size: 761 1341 1761
Fragments arranged by size:
1761 1341 761
BclI T'GATC_A
Cuts at: 1383 1383
Size: 3863
BglI GCCn_nnn'nGGC
Cuts at: 428 428
Size: 3863
BglII A'GATC_T
Cuts at: 1378 3415 1378
Size: 2037 1826
BlpI GC'TnA_GC
Cuts at: 975 975
Size: 3863
Bme1580I G_kGCm'C
Cuts at: 517 1488 1581 2637 517
Size: 971 93 1056 1743
Fragments arranged by size:
1743 1056 971 93
BmrI ACTGGGnnnn_n'
Cuts at: 657 817 1075 1156 1491 3635 657
Size: 160 258 81 335 2144 885
Fragments arranged by size:
2144 885 335 258 160 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 3863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 320 329 356 269
Size: 51 9 27 3776
Fragments arranged by size:
3776 51 27 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 1039 1918 2410 2708 149
Size: 890 879 492 298 1304
Fragments arranged by size:
1304 890 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 3863
BsaAI yAC'GTr
Cuts at: 1216 1216
Size: 3863
BsaBI GATnn'nnATC
Cuts at: 6 1377 3414 6
Size: 1371 2037 455
Fragments arranged by size:
2037 1371 455
BsaHI Gr'CG_yC
Cuts at: 290 431 1294 1542 2244 3740 290
Size: 141 863 248 702 1496 413
Fragments arranged by size:
1496 863 702 413 248 141
BsaWI w'CCGG_w
Cuts at: 400 1052 1573 2251 2525 2672 3083 400
Size: 652 521 678 274 147 411 1180
Fragments arranged by size:
1180 678 652 521 411 274 147
BseYI C'CCAG_C
Cuts at: 199 355 439 1288 2046 2623 3119 199
Size: 156 84 849 758 577 496 943
Fragments arranged by size:
943 849 758 577 496 156 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 314
Size: 3863
BsiEI CG_ry'CG
Cuts at: 104 1405 1451 2659 104
Size: 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 46
BsiHKAI G_wGCw'C
Cuts at: 306 327 877 1655 1845 2637 306
Size: 21 550 778 190 792 1532
Fragments arranged by size:
1532 792 778 550 190 21
Bsp1286I G_dGCh'C
Cuts at: 306 327 517 877 1488 1581 1655 1845 1907
Size: 21 190 360 611 93 74 190 62
Cuts at: 1907 2637 306
Size: 730 1532
Fragments arranged by size:
1532 730 611 360 190 190 93 74
62 21
BspEI T'CCGG_A
Cuts at: 3083 3083
Size: 3863
BspHI T'CATG_A
Cuts at: 3039 3039
Size: 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 473 913 1127 1429 1810 336
Size: 137 440 214 302 381 2389
Fragments arranged by size:
2389 440 381 302 214 137
BsrBI CCG'CTC
Cuts at: 50 2155 2209 3693 3858 50
Size: 2105 54 1484 165 55
Fragments arranged by size:
2105 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1775 1775
Size: 3863
BsrFI r'CCGG_y
Cuts at: 1861 2042 2251 1861
Size: 181 209 3473
Fragments arranged by size:
3473 209 181
BssSI C'ACGA_G
Cuts at: 2134 2492 2134
Size: 358 3505
BstAPI GCAn_nnn'nTGC
Cuts at: 1240 1240
Size: 3863
BstBI TT'CG_AA
Cuts at: 2224 2224
Size: 3863
BstXI CCAn_nnnn'nTGG
Cuts at: 199 199
Size: 3863
BstYI r'GATC_y
Cuts at: 1 888 1355 1378 1713 1959 2971 3057 3069
Size: 887 467 23 335 246 1012 86 12
Cuts at: 3069 3415 1
Size: 346 449
Fragments arranged by size:
1012 887 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3863
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1813 1994 2157 1813
Size: 181 163 3519
Fragments arranged by size:
3519 181 163
BtsI GCAGTG_nn'
Cuts at: 749 1106 749
Size: 357 3506
DraI TTT'AAA
Cuts at: 862 862
Size: 3863
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2427 1569
Size: 858 3005
EaeI y'GGCC_r
Cuts at: 1448 1622 2013 2040 3385 1448
Size: 174 391 27 1345 1926
Fragments arranged by size:
1926 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1448 1448
Size: 3863
EarI CTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2071 2381 2527 3673 2071
Size: 310 146 1146 2261
Fragments arranged by size:
2261 1146 310 146
EcoICRI GAG'CTC
Cuts at: 875 875
Size: 3863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 320 329 356 1688 2120 2867 3135 269
Size: 51 9 27 1332 432 747 268 997
Fragments arranged by size:
1332 997 747 432 268 51 27 9
EcoO109I rG'GnC_Cy
Cuts at: 236 368 1002 236
Size: 132 634 3097
Fragments arranged by size:
3097 634 132
EcoRI G'AATT_C
Cuts at: 867 867
Size: 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3863
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3863
HaeII r_GCGC'y
Cuts at: 293 434 1297 1545 2567 3546 293
Size: 141 863 248 1022 979 610
Fragments arranged by size:
1022 979 863 610 248 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 1705 1735 1737 1767 124
Size: 32 1549 30 2 30 2220
Fragments arranged by size:
2220 1549 32 30 30 2
HincII GTy'rAC
Cuts at: 902 902
Size: 3863
KasI G'GCGC_C
Cuts at: 289 430 1293 1541 289
Size: 141 863 248 2611
Fragments arranged by size:
2611 863 248 141
KpnI G_GTAC'C
Cuts at: 883 883
Size: 3863
MluI A'CGCG_T
Cuts at: 3296 3296
Size: 3863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2718 2534
Size: 184 3679
MscI TGG'CCA
Cuts at: 1624 1624
Size: 3863
MslI CAynn'nnrTG
Cuts at: 1852 1979 1852
Size: 127 3736
MspA1I CmG'CkG
Cuts at: 359 974 1288 1648 2661 2906 359
Size: 615 314 360 1013 245 1316
Fragments arranged by size:
1316 1013 615 360 314 245
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 3863
NarI GG'CG_CC
Cuts at: 290 431 1294 1542 290
Size: 141 863 248 2611
Fragments arranged by size:
2611 863 248 141
NcoI C'CATG_G
Cuts at: 107 107
Size: 3863
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 3863
NspI r_CATG'y
Cuts at: 498 916 1416 2323 498
Size: 418 500 907 2038
Fragments arranged by size:
2038 907 500 418
PasI CC'CwG_GG
Cuts at: 217 259 346 364 217
Size: 42 87 18 3716
Fragments arranged by size:
3716 87 42 18
PciI A'CATG_T
Cuts at: 2319 2319
Size: 3863
PflMI CCAn_nnn'nTGG
Cuts at: 382 382
Size: 3863
PfoI T'CCnGG_A
Cuts at: 270 318 270
Size: 48 3815
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3034 3002
Size: 32 3831
PsiI TTA'TAA
Cuts at: 461 461
Size: 3863
PstI C_TGCA'G
Cuts at: 910 1595 910
Size: 685 3178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3863
PvuII CAG'CTG
Cuts at: 1288 1648 1288
Size: 360 3503
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3863
SacI G_AGCT'C
Cuts at: 877 877
Size: 3863
SalI G'TCGA_C
Cuts at: 900 900
Size: 3863
SapI GCTCTTCn'nnn_
Cuts at: 1886 2096 1886
Size: 210 3653
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3863
ScaI AGT'ACT
Cuts at: 713 3380 713
Size: 2667 1196
SfcI C'TryA_G
Cuts at: 33 906 1591 2584 2775 33
Size: 873 685 993 191 1121
Fragments arranged by size:
1121 993 873 685 191
SfoI GGC'GCC
Cuts at: 291 432 1295 1543 291
Size: 141 863 248 2611
Fragments arranged by size:
2611 863 248 141
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
SmaI CCC'GGG
Cuts at: 246 885 246
Size: 639 3224
SmlI C'TyrA_G
Cuts at: 164 1018 1933 2425 2687 164
Size: 854 915 492 262 1340
Fragments arranged by size:
1340 915 854 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3863
SphI G_CATG'C
Cuts at: 498 916 1416 498
Size: 418 500 2945
Fragments arranged by size:
2945 500 418
StyI C'CwwG_G
Cuts at: 107 997 1351 107
Size: 890 354 2619
Fragments arranged by size:
2619 890 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 369 1446 1579 2038 2247 369
Size: 1077 133 459 209 1985
Fragments arranged by size:
1985 1077 459 209 133
TatI w'GTAC_w
Cuts at: 711 767 3378 711
Size: 56 2611 1196
Fragments arranged by size:
2611 1196 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 3831
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3863
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1358 1358
Size: 3863
XmaI C'CCGG_G
Cuts at: 244 883 244
Size: 639 3224
XmnI GAAnn'nnTTC
Cuts at: 697 697
Size: 3863
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI