Restriction map for
pF1KB6306
(Circular) (Six-base) MAPSORT of: pF1KB6306.seq Check: 607 from: 1 to: 3953
pF1KB6306 3953 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 358 358
Size: 3953
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
AfeI AGC'GCT
Cuts at: 325 325
Size: 3953
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AleI CACnn'nnGTG
Cuts at: 765 765
Size: 3953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 783 783
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 500 513 776 1258 2825 500
Size: 13 263 482 1567 1628
Fragments arranged by size:
1628 1567 482 263 13
ApaI G_GGCC'C
Cuts at: 947 947
Size: 3953
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 957 3306 3562 957
Size: 2349 256 1348
Fragments arranged by size:
2349 1348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 258 713 973 258
Size: 455 260 3238
Fragments arranged by size:
3238 455 260
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 839 969 1383 1631 1666 839
Size: 130 414 248 35 3126
Fragments arranged by size:
3126 414 248 130 35
BanII G_rGCy'C
Cuts at: 520 947 967 1997 520
Size: 427 20 1030 2476
Fragments arranged by size:
2476 1030 427 20
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbsI GAAGACnn'nnnn_
Cuts at: 284 833 284
Size: 549 3404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 526 560 526
Size: 34 3919
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 639 1473 639
Size: 834 3119
BglII A'GATC_T
Cuts at: 1468 3505 1468
Size: 2037 1916
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 197 536 563 947 1578 1671 2727 197
Size: 339 27 384 631 93 1056 1423
Fragments arranged by size:
1423 1056 631 384 339 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 523 1165 1246 1581 3725 523
Size: 642 81 335 2144 751
Fragments arranged by size:
2144 751 642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 902 3657 902
Size: 2755 1198
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 722 1467 3504 6
Size: 716 745 2037 455
Fragments arranged by size:
2037 745 716 455
BsaHI Gr'CG_yC
Cuts at: 685 1384 1632 2334 3830 685
Size: 699 248 702 1496 808
Fragments arranged by size:
1496 808 702 699 248
BsaWI w'CCGG_w
Cuts at: 673 900 1142 1663 2341 2615 2762 3173 673
Size: 227 242 521 678 274 147 411 1453
Fragments arranged by size:
1453 678 521 411 274 242 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 899 929 838
Size: 30 31 30 3862
Fragments arranged by size:
3862 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 575 575
Size: 3953
BseYI C'CCAG_C
Cuts at: 1378 2136 2713 3209 1378
Size: 758 577 496 2122
Fragments arranged by size:
2122 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 789 513
Size: 276 3677
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 412 482 549 920 967 1745 1935 2727 412
Size: 70 67 371 47 778 190 792 1638
Fragments arranged by size:
1638 792 778 371 190 70 67 47
Bsp1286I G_dGCh'C
Cuts at: 197 412 482 520 536 549 563 920 947
Size: 215 70 38 16 13 14 357 27
Cuts at: 947 967 1578 1671 1745 1935 1997 2727 197
Size: 20 611 93 74 190 62 730 1423
Fragments arranged by size:
1423 730 611 357 215 190 93 74
70 62 38 27 20 16 14 13
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 358 1003 1217 1519 1900 358
Size: 645 214 302 381 2411
Fragments arranged by size:
2411 645 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 381 1865 381
Size: 1484 2469
BsrFI r'CCGG_y
Cuts at: 1951 2132 2341 1951
Size: 181 209 3563
Fragments arranged by size:
3563 209 181
BssSI C'ACGA_G
Cuts at: 292 2224 2582 292
Size: 1932 358 1663
Fragments arranged by size:
1932 1663 358
BstAPI GCAn_nnn'nTGC
Cuts at: 380 776 1330 380
Size: 396 554 3003
Fragments arranged by size:
3003 554 396
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstYI r'GATC_y
Cuts at: 1 723 978 1445 1468 1803 2049 3061 3147
Size: 722 255 467 23 335 246 1012 86
Cuts at: 3147 3159 3505 1
Size: 12 346 449
Fragments arranged by size:
1012 722 467 449 346 335 255 246
86 23 12
BtgI C'CryG_G
Cuts at: 646 646
Size: 3953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 396 412 480 501 1196 396
Size: 16 68 21 695 3153
Fragments arranged by size:
3153 695 68 21 16
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DraIII CAC_nnn'GTG
Cuts at: 622 622
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 1538 1712 2103 2130 3475 1538
Size: 174 391 27 1345 2016
Fragments arranged by size:
2016 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 440 943 1092 440
Size: 503 149 3301
Fragments arranged by size:
3301 503 149
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 327 786 1387 1635 2657 3636 327
Size: 459 601 248 1022 979 644
Fragments arranged by size:
1022 979 644 601 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1795 1825 1827 1857 1795
Size: 30 2 30 3891
Fragments arranged by size:
3891 30 30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 765 1942 2069 765
Size: 1177 127 2649
Fragments arranged by size:
2649 1177 127
MspA1I CmG'CkG
Cuts at: 179 308 317 599 776 866 1064 1378 1738
Size: 129 9 282 177 90 198 314 360
Cuts at: 1738 2751 2996 179
Size: 1013 245 1136
Fragments arranged by size:
1136 1013 360 314 282 245 198 177
129 90 9
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 646 646
Size: 3953
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PfoI T'CCnGG_A
Cuts at: 442 442
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PpuMI rG'GwC_Cy
Cuts at: 440 440
Size: 3953
PspOMI G'GGCC_C
Cuts at: 943 943
Size: 3953
PspXI vC'TCGA_Gb
Cuts at: 258 258
Size: 3953
PstI C_TGCA'G
Cuts at: 391 604 1000 1685 391
Size: 213 396 685 2659
Fragments arranged by size:
2659 685 396 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 776 1378 1738 776
Size: 602 360 2991
Fragments arranged by size:
2991 602 360
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SanDI GG'GwC_CC
Cuts at: 440 440
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 387 600 930 996 1681 2674 2865 33
Size: 354 213 330 66 685 993 191 1121
Fragments arranged by size:
1121 993 685 354 330 213 191 66
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 715 975 715
Size: 260 3693
SmlI C'TyrA_G
Cuts at: 258 1108 2023 2515 2777 258
Size: 850 915 492 262 1434
Fragments arranged by size:
1434 915 850 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 646 1087 1441 646
Size: 441 354 3158
Fragments arranged by size:
3158 441 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 845 1536 1669 2128 2337 845
Size: 691 133 459 209 2461
Fragments arranged by size:
2461 691 459 209 133
TatI w'GTAC_w
Cuts at: 161 3468 161
Size: 3307 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 899 931 3470 3502 899
Size: 32 2539 32 1350
Fragments arranged by size:
2539 1350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XhoI C'TCGA_G
Cuts at: 258 258
Size: 3953
XmaI C'CCGG_G
Cuts at: 713 973 713
Size: 260 3693
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BglI BmgBI BmtI BpmI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI