Restriction map for
pF1KB6308
(Circular) (Six-base) MAPSORT of: pF1KB6308.seq Check: 5069 from: 1 to: 3953
pF1KB6308 3953 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 907 1778 2210 2957 3225 812
Size: 95 871 432 747 268 1540
Fragments arranged by size:
1540 871 747 432 268 95
AflIII A'CryG_T
Cuts at: 914 1305 2409 3386 914
Size: 391 1104 977 1481
Fragments arranged by size:
1481 1104 977 391
AgeI A'CCGG_T
Cuts at: 552 2341 552
Size: 1789 2164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 542 574 542
Size: 32 3921
AlwNI CAG_nnn'CTG
Cuts at: 568 1258 2825 568
Size: 690 1567 1696
Fragments arranged by size:
1696 1567 690
ApaLI G'TGCA_C
Cuts at: 796 2723 796
Size: 1927 2026
ApoI r'AATT_y
Cuts at: 304 678 957 3306 3562 304
Size: 374 279 2349 256 695
Fragments arranged by size:
2349 695 374 279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 374 973 374
Size: 599 3354
AvrII C'CTAG_G
Cuts at: 523 523
Size: 3953
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 969 1383 1631 1666 969
Size: 414 248 35 3256
Fragments arranged by size:
3256 414 248 35
BanII G_rGCy'C
Cuts at: 967 1997 967
Size: 1030 2923
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbsI GAAGACnn'nnnn_
Cuts at: 391 604 917 391
Size: 213 313 3427
Fragments arranged by size:
3427 313 213
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglII A'GATC_T
Cuts at: 726 1468 3505 726
Size: 742 2037 1174
Fragments arranged by size:
2037 1174 742
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 800 1578 1671 2727 800
Size: 778 93 1056 2026
Fragments arranged by size:
2026 1056 778 93
BmgBI CAC'GTC
Cuts at: 915 915
Size: 3953
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 2320 339
Size: 1981 1972
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 456 3657 456
Size: 3201 752
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 552 1142 1663 2341 2615 2762 3173 552
Size: 590 521 678 274 147 411 1332
Fragments arranged by size:
1332 678 590 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 272 272
Size: 3953
BseYI C'CCAG_C
Cuts at: 1378 2136 2713 3209 1378
Size: 758 577 496 2122
Fragments arranged by size:
2122 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 180
Size: 3953
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 800 967 1745 1935 2727 800
Size: 167 778 190 792 2026
Fragments arranged by size:
2026 792 778 190 167
BsmI GAATG_Cn'
Cuts at: 402 402
Size: 3953
Bsp1286I G_dGCh'C
Cuts at: 800 967 1578 1671 1745 1935 1997 2727 800
Size: 167 611 93 74 190 62 730 2026
Fragments arranged by size:
2026 730 611 190 167 93 74 62
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 906 3129 906
Size: 2223 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 1003 1217 1519 1900 202
Size: 801 214 302 381 2255
Fragments arranged by size:
2255 801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 552 1951 2132 2341 552
Size: 1399 181 209 2164
Fragments arranged by size:
2164 1399 209 181
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 129 129
Size: 3953
BstYI r'GATC_y
Cuts at: 1 564 726 978 1445 1468 1803 2049 3061
Size: 563 162 252 467 23 335 246 1012
Cuts at: 3061 3147 3159 3505 1
Size: 86 12 346 449
Fragments arranged by size:
1012 563 467 449 346 335 252 246
162 86 23 12
BtgI C'CryG_G
Cuts at: 455 455
Size: 3953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
DraI TTT'AAA
Cuts at: 723 952 723
Size: 229 3724
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 1538 1712 2103 2130 3475 1538
Size: 174 391 27 1345 2016
Fragments arranged by size:
2016 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 601 1976 2186 601
Size: 1375 210 2368
Fragments arranged by size:
2368 1375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 907 1778 2210 2957 3225 812
Size: 95 871 432 747 268 1540
Fragments arranged by size:
1540 871 747 432 268 95
EcoO109I rG'GnC_Cy
Cuts at: 137 1092 137
Size: 955 2998
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 1972 243
Size: 1729 2224
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 1387 1635 2657 3636 1387
Size: 248 1022 979 1704
Fragments arranged by size:
1704 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 451 483 1795 1825 1827 1857 343
Size: 32 76 32 1312 30 2 30 2439
Fragments arranged by size:
2439 1312 76 32 32 30 30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
HindIII A'AGCT_T
Cuts at: 181 220 181
Size: 39 3914
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 514 1942 2069 514
Size: 1428 127 2398
Fragments arranged by size:
2398 1428 127
MspA1I CmG'CkG
Cuts at: 1064 1378 1738 2751 2996 1064
Size: 314 360 1013 245 2021
Fragments arranged by size:
2021 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 270 1006 1506 2413 270
Size: 736 500 907 1810
Fragments arranged by size:
1810 907 736 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PstI C_TGCA'G
Cuts at: 330 1000 1685 330
Size: 670 685 2598
Fragments arranged by size:
2598 685 670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 1378 1738 1378
Size: 360 3593
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 326 820 996 1681 2674 2865 33
Size: 293 494 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 494 293 191 176
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 374 1108 2023 2515 2777 374
Size: 734 915 492 262 1550
Fragments arranged by size:
1550 915 734 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
SspI AAT'ATT
Cuts at: 870 870
Size: 3953
StuI AGG'CCT
Cuts at: 859 859
Size: 3953
StyI C'CwwG_G
Cuts at: 523 1087 1441 523
Size: 564 354 3035
Fragments arranged by size:
3035 564 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 775 1536 1669 2128 2337 775
Size: 761 133 459 209 2391
Fragments arranged by size:
2391 761 459 209 133
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 3921
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XhoI C'TCGA_G
Cuts at: 374 374
Size: 3953
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
XmnI GAAnn'nnTTC
Cuts at: 151 295 151
Size: 144 3809
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI ZraI