Restriction map for
pF1KB6309
(Circular) (Six-base) MAPSORT of: pF1KB6309.seq Check: 5016 from: 1 to: 3887
pF1KB6309 3887 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 580 580
Size: 3887
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1712 2144 2891 3159 763
Size: 949 432 747 268 1491
Fragments arranged by size:
1491 949 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 219 219
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 159 1192 2759 159
Size: 1033 1567 1287
Fragments arranged by size:
1567 1287 1033
ApaI G_GGCC'C
Cuts at: 219 559 219
Size: 340 3547
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 123 144 451 907 123
Size: 21 307 456 3103
Fragments arranged by size:
3103 456 307 21
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 903 1317 1565 1600 903
Size: 414 248 35 3190
Fragments arranged by size:
3190 414 248 35
BanII G_rGCy'C
Cuts at: 219 559 901 1931 219
Size: 340 342 1030 2175
Fragments arranged by size:
2175 1030 342 340
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BbvCI CC'TCA_GC
Cuts at: 476 476
Size: 3887
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 437 1407 437
Size: 970 2917
BglI GCCn_nnn'nGGC
Cuts at: 118 245 118
Size: 127 3760
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 315 999 315
Size: 684 3203
Bme1580I G_kGCm'C
Cuts at: 219 359 559 860 1512 1605 2661 219
Size: 140 200 301 652 93 1056 1445
Fragments arranged by size:
1445 1056 652 301 200 140 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BmtI G_CTAG'C
Cuts at: 175 271 314 175
Size: 96 43 3748
Fragments arranged by size:
3748 96 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 740 393
Size: 347 3540
Bpu10I CC'TnA_GC
Cuts at: 476 476
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 380 1240 380
Size: 860 3027
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 392 1076 1597 2275 2549 2696 3107 392
Size: 684 521 678 274 147 411 1172
Fragments arranged by size:
1172 684 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 481
Size: 3887
BseYI C'CCAG_C
Cuts at: 159 188 330 1312 2070 2647 3143 159
Size: 29 142 982 758 577 496 903
Fragments arranged by size:
982 903 758 577 496 142 29
BsiEI CG_ry'CG
Cuts at: 104 839 1429 1475 2683 104
Size: 735 590 46 1208 1308
Fragments arranged by size:
1308 1208 735 590 46
BsiHKAI G_wGCw'C
Cuts at: 379 901 1679 1869 2661 379
Size: 522 778 190 792 1605
Fragments arranged by size:
1605 792 778 522 190
BsiWI C'GTAC_G
Cuts at: 380 380
Size: 3887
BsmI GAATG_Cn'
Cuts at: 505 693 870 505
Size: 188 177 3522
Fragments arranged by size:
3522 188 177
BsmBI CGTCTCn'nnnn_
Cuts at: 264 264
Size: 3887
Bsp1286I G_dGCh'C
Cuts at: 219 359 379 559 860 901 1512 1605 1679
Size: 140 20 180 301 41 611 93 74
Cuts at: 1679 1869 1931 2661 219
Size: 190 62 730 1445
Fragments arranged by size:
1445 730 611 301 190 180 140 93
74 62 41 20
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 580 937 1151 1453 1834 545
Size: 35 357 214 302 381 2598
Fragments arranged by size:
2598 381 357 302 214 35
BsrBI CCG'CTC
Cuts at: 50 609 2179 2233 3717 3882 50
Size: 559 1570 54 1484 165 55
Fragments arranged by size:
1570 1484 559 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 117 1885 2066 2275 117
Size: 1768 181 209 1729
Fragments arranged by size:
1768 1729 209 181
BssHII G'CGCG_C
Cuts at: 192 319 192
Size: 127 3760
BssSI C'ACGA_G
Cuts at: 352 2158 2516 352
Size: 1806 358 1723
Fragments arranged by size:
1806 1723 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DraIII CAC_nnn'GTG
Cuts at: 661 661
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 110 836 1472 1646 2037 2064 3409 110
Size: 726 636 174 391 27 1345 588
Fragments arranged by size:
1345 726 636 588 391 174 27
EagI C'GGCC_G
Cuts at: 836 1472 836
Size: 636 3251
EarI CTCTTCn'nnn_
Cuts at: 586 1910 2120 586
Size: 1324 210 2353
Fragments arranged by size:
2353 1324 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 740 763 1712 2144 2891 3159 393
Size: 347 23 949 432 747 268 1121
Fragments arranged by size:
1121 949 747 432 347 268 23
EcoNI CCTnn'n_nnAGG
Cuts at: 601 601
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 215 556 1026 215
Size: 341 470 3076
Fragments arranged by size:
3076 470 341
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 172 351 1321 1569 2591 3570 172
Size: 179 970 248 1022 979 489
Fragments arranged by size:
1022 979 970 489 248 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 404 436 609 641 1729 1759 1761 1791 404
Size: 32 173 32 1088 30 2 30 2500
Fragments arranged by size:
2500 1088 173 32 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 136 709 1876 2003 136
Size: 573 1167 127 2020
Fragments arranged by size:
2020 1167 573 127
MspA1I CmG'CkG
Cuts at: 180 736 777 998 1312 1672 2685 2930 180
Size: 556 41 221 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 556 360 314 245 221 41
NaeI GCC'GGC
Cuts at: 119 2068 119
Size: 1949 1938
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NcoI C'CATG_G
Cuts at: 107 107
Size: 3887
NgoMIV G'CCGG_C
Cuts at: 117 2066 117
Size: 1949 1938
NheI G'CTAG_C
Cuts at: 171 267 310 171
Size: 96 43 3748
Fragments arranged by size:
3748 96 43
NotI GC'GGCC_GC
Cuts at: 836 836
Size: 3887
NspI r_CATG'y
Cuts at: 203 940 1440 2347 203
Size: 737 500 907 1743
Fragments arranged by size:
1743 907 737 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 159 159
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PspOMI G'GGCC_C
Cuts at: 215 555 215
Size: 340 3547
PstI C_TGCA'G
Cuts at: 934 1619 934
Size: 685 3202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 180 736 1312 1672 180
Size: 556 576 360 2395
Fragments arranged by size:
2395 576 556 360
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 601 930 1615 2608 2799 33
Size: 568 329 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 329 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 118 118
Size: 3887
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 125 909 125
Size: 784 3103
SmlI C'TyrA_G
Cuts at: 144 1042 1957 2449 2711 144
Size: 898 915 492 262 1320
Fragments arranged by size:
1320 915 898 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 203 940 1440 203
Size: 737 500 2650
Fragments arranged by size:
2650 737 500
StyI C'CwwG_G
Cuts at: 107 510 1021 1375 107
Size: 403 511 354 2619
Fragments arranged by size:
2619 511 403 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 1470 1603 2062 2271 151
Size: 1319 133 459 209 1767
Fragments arranged by size:
1767 1319 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XhoI C'TCGA_G
Cuts at: 144 144
Size: 3887
XmaI C'CCGG_G
Cuts at: 123 907 123
Size: 784 3103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BsaXI BsgI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI