Restriction map for
pF1KB6320
(Circular) (Six-base) MAPSORT of: pF1KB6320.seq Check: 2949 from: 1 to: 3959
pF1KB6320 3959 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 997 997
Size: 3959
Acc65I G'GTAC_C
Cuts at: 825 975 825
Size: 150 3809
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1784 2216 2963 3231 274
Size: 1510 432 747 268 1002
Fragments arranged by size:
1510 1002 747 432 268
AflIII A'CryG_T
Cuts at: 1311 2415 3392 1311
Size: 1104 977 1878
Fragments arranged by size:
1878 1104 977
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AleI CACnn'nnGTG
Cuts at: 409 502 409
Size: 93 3866
AlwNI CAG_nnn'CTG
Cuts at: 228 1264 2831 228
Size: 1036 1567 1356
Fragments arranged by size:
1567 1356 1036
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 963 3312 3568 963
Size: 2349 256 1354
Fragments arranged by size:
2349 1354 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 118 531 829 979 118
Size: 413 298 150 3098
Fragments arranged by size:
3098 413 298 150
BamHI G'GATC_C
Cuts at: 984 984
Size: 3959
BanI G'GyrC_C
Cuts at: 403 555 825 975 1389 1637 1672 403
Size: 152 270 150 414 248 35 2690
Fragments arranged by size:
2690 414 270 248 152 150 35
BanII G_rGCy'C
Cuts at: 773 973 2003 773
Size: 200 1030 2729
Fragments arranged by size:
2729 1030 200
BbeI G_GCGC'C
Cuts at: 559 1393 1641 559
Size: 834 248 2877
Fragments arranged by size:
2877 834 248
BciVI GTATCCnnnnn_n'
Cuts at: 367 1857 2618 3959 367
Size: 1490 761 1341 367
Fragments arranged by size:
1490 1341 761 367
BclI T'GATC_A
Cuts at: 1479 1479
Size: 3959
BglII A'GATC_T
Cuts at: 935 1474 3511 935
Size: 539 2037 1383
Fragments arranged by size:
2037 1383 539
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 1584 1677 2733 1584
Size: 93 1056 2810
Fragments arranged by size:
2810 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 207 1171 1252 1587 3731 207
Size: 964 81 335 2144 435
Fragments arranged by size:
2144 964 435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2014 2506 2804 1135
Size: 879 492 298 2290
Fragments arranged by size:
2290 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 115 451 754 3663 115
Size: 336 303 2909 411
Fragments arranged by size:
2909 411 336 303
BsaAI yAC'GTr
Cuts at: 1312 1312
Size: 3959
BsaBI GATnn'nnATC
Cuts at: 6 1473 3510 6
Size: 1467 2037 455
Fragments arranged by size:
2037 1467 455
BsaHI Gr'CG_yC
Cuts at: 556 1390 1638 2340 3836 556
Size: 834 248 702 1496 679
Fragments arranged by size:
1496 834 702 679 248
BsaWI w'CCGG_w
Cuts at: 1148 1669 2347 2621 2768 3179 1148
Size: 521 678 274 147 411 1928
Fragments arranged by size:
1928 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 772
Size: 3959
BseYI C'CCAG_C
Cuts at: 701 1384 2142 2719 3215 701
Size: 683 758 577 496 1445
Fragments arranged by size:
1445 758 683 577 496
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 773 973 1751 1941 2733 773
Size: 200 778 190 792 1999
Fragments arranged by size:
1999 792 778 200 190
Bsp1286I G_dGCh'C
Cuts at: 773 973 1584 1677 1751 1941 2003 2733 773
Size: 200 611 93 74 190 62 730 1999
Fragments arranged by size:
1999 730 611 200 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 758 1009 1223 1525 1906 758
Size: 251 214 302 381 2811
Fragments arranged by size:
2811 381 302 251 214
BsrBI CCG'CTC
Cuts at: 50 417 2251 2305 3789 3954 50
Size: 367 1834 54 1484 165 55
Fragments arranged by size:
1834 1484 367 165 55 54
BsrDI GCAATG_nn'
Cuts at: 386 1871 386
Size: 1485 2474
BsrFI r'CCGG_y
Cuts at: 1957 2138 2347 1957
Size: 181 209 3569
Fragments arranged by size:
3569 209 181
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 576 678 1336 576
Size: 102 658 3199
Fragments arranged by size:
3199 658 102
BstBI TT'CG_AA
Cuts at: 2320 2320
Size: 3959
BstXI CCAn_nnnn'nTGG
Cuts at: 504 504
Size: 3959
BstYI r'GATC_y
Cuts at: 1 224 644 935 984 1451 1474 1809 2055
Size: 223 420 291 49 467 23 335 246
Cuts at: 2055 3067 3153 3165 3511 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 420 346 335 291 246
223 86 49 23 12
BtgI C'CryG_G
Cuts at: 107 503 107
Size: 396 3563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 828 905 1909 2090 2253 828
Size: 77 1004 181 163 2534
Fragments arranged by size:
2534 1004 181 163 77
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 1665 2523 1665
Size: 858 3101
EaeI y'GGCC_r
Cuts at: 219 464 639 1544 1718 2109 2136 3481 219
Size: 245 175 905 174 391 27 1345 697
Fragments arranged by size:
1345 905 697 391 245 175 174 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 430 1982 2192 430
Size: 1552 210 2197
Fragments arranged by size:
2197 1552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2167 2477 2623 3769 2167
Size: 310 146 1146 2357
Fragments arranged by size:
2357 1146 310 146
EcoICRI GAG'CTC
Cuts at: 771 971 771
Size: 200 3759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1784 2216 2963 3231 274
Size: 1510 432 747 268 1002
Fragments arranged by size:
1510 1002 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 754 754
Size: 3959
EcoO109I rG'GnC_Cy
Cuts at: 523 1098 523
Size: 575 3384
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
EcoRV GAT'ATC
Cuts at: 234 384 234
Size: 150 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 957 1978 957
Size: 1021 2938
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 302 559 1393 1641 2663 3642 302
Size: 257 834 248 1022 979 619
Fragments arranged by size:
1022 979 834 619 257 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1801 1831 1833 1863 1801
Size: 30 2 30 3897
Fragments arranged by size:
3897 30 30 2
HincII GTy'rAC
Cuts at: 998 998
Size: 3959
KasI G'GCGC_C
Cuts at: 555 1389 1637 555
Size: 834 248 2877
Fragments arranged by size:
2877 834 248
KpnI G_GTAC'C
Cuts at: 829 979 829
Size: 150 3809
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2630 2814 2630
Size: 184 3775
MscI TGG'CCA
Cuts at: 221 641 1720 221
Size: 420 1079 2460
Fragments arranged by size:
2460 1079 420
MslI CAynn'nnrTG
Cuts at: 409 502 1948 2075 409
Size: 93 1446 127 2293
Fragments arranged by size:
2293 1446 127 93
MspA1I CmG'CkG
Cuts at: 1070 1384 1744 2757 3002 1070
Size: 314 360 1013 245 2027
Fragments arranged by size:
2027 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 556 1390 1638 556
Size: 834 248 2877
Fragments arranged by size:
2877 834 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3959
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NruI TCG'CGA
Cuts at: 890 890
Size: 3959
NspI r_CATG'y
Cuts at: 540 579 600 1012 1512 2419 540
Size: 39 21 412 500 907 2080
Fragments arranged by size:
2080 907 500 412 39 21
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PflMI CCAn_nnn'nTGG
Cuts at: 228 228
Size: 3959
PfoI T'CCnGG_A
Cuts at: 227 227
Size: 3959
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3927
PstI C_TGCA'G
Cuts at: 902 1006 1691 902
Size: 104 685 3170
Fragments arranged by size:
3170 685 104
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 1384 1744 1384
Size: 360 3599
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 773 973 773
Size: 200 3759
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
SbfI CC_TGCA'GG
Cuts at: 902 1006 902
Size: 104 3855
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SfcI C'TryA_G
Cuts at: 33 898 1002 1687 2680 2871 33
Size: 865 104 685 993 191 1121
Fragments arranged by size:
1121 993 865 685 191 104
SfoI GGC'GCC
Cuts at: 557 1391 1639 557
Size: 834 248 2877
Fragments arranged by size:
2877 834 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 533 831 981 533
Size: 298 150 3511
Fragments arranged by size:
3511 298 150
SmlI C'TyrA_G
Cuts at: 1114 2029 2521 2783 1114
Size: 915 492 262 2290
Fragments arranged by size:
2290 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 540 1012 1512 540
Size: 472 500 2987
Fragments arranged by size:
2987 500 472
StuI AGG'CCT
Cuts at: 271 629 271
Size: 358 3601
StyI C'CwwG_G
Cuts at: 107 1093 1447 107
Size: 986 354 2619
Fragments arranged by size:
2619 986 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 388 1542 1675 2134 2343 388
Size: 1154 133 459 209 2004
Fragments arranged by size:
2004 1154 459 209 133
TatI w'GTAC_w
Cuts at: 3474 3474
Size: 3959
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 3927
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 504 1454 504
Size: 950 3009
XmaI C'CCGG_G
Cuts at: 531 829 979 531
Size: 298 150 3511
Fragments arranged by size:
3511 298 150
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI