Restriction map for
pF1KB6321
(Circular) (Six-base) MAPSORT of: pF1KB6321.seq Check: 2529 from: 1 to: 3896
pF1KB6321 3896 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AfeI AGC'GCT
Cuts at: 446 612 446
Size: 166 3730
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 215 299 900 3249 3505 215
Size: 84 601 2349 256 606
Fragments arranged by size:
2349 606 601 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 202 912 1326 1574 1609 202
Size: 710 414 248 35 2489
Fragments arranged by size:
2489 710 414 248 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 815 1416 815
Size: 601 3295
BfrBI ATG'CAT
Cuts at: 596 596
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 848 1008 848
Size: 160 3736
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 131 111
Size: 20 3876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 521 1321 2079 2656 3152 521
Size: 800 758 577 496 1265
Fragments arranged by size:
1265 800 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 910 1688 1878 2670 910
Size: 778 190 792 2136
Fragments arranged by size:
2136 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 246 246
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 910 1521 1614 1688 1878 1940 2670 910
Size: 611 93 74 190 62 730 2136
Fragments arranged by size:
2136 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 383 505 1808 383
Size: 122 1303 2471
Fragments arranged by size:
2471 1303 122
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BsrGI T'GTAC_A
Cuts at: 512 512
Size: 3896
BssSI C'ACGA_G
Cuts at: 826 2167 2525 826
Size: 1341 358 2197
Fragments arranged by size:
2197 1341 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 179 707 179
Size: 528 3368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 336 1139 336
Size: 803 3093
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 579 614 579
Size: 35 3861
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 666 1602 2460 666
Size: 936 858 2102
Fragments arranged by size:
2102 936 858
EaeI y'GGCC_r
Cuts at: 1481 1655 2046 2073 3418 1481
Size: 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 131 1721 2153 2900 3168 111
Size: 20 1590 432 747 268 839
Fragments arranged by size:
1590 839 747 432 268 20
EcoO109I rG'GnC_Cy
Cuts at: 1035 1035
Size: 3896
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
EcoRV GAT'ATC
Cuts at: 456 456
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 448 614 1330 1578 2600 3579 448
Size: 166 716 248 1022 979 765
Fragments arranged by size:
1022 979 765 716 248 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 245 277 1738 1768 1770 1800 245
Size: 32 1461 30 2 30 2341
Fragments arranged by size:
2341 1461 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
HindIII A'AGCT_T
Cuts at: 268 464 268
Size: 196 3700
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 1885 2012 1885
Size: 127 3769
MspA1I CmG'CkG
Cuts at: 619 1007 1321 1681 2694 2939 619
Size: 388 314 360 1013 245 1576
Fragments arranged by size:
1576 1013 388 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 179 707 179
Size: 528 3368
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NsiI A_TGCA'T
Cuts at: 598 598
Size: 3896
NspI r_CATG'y
Cuts at: 199 596 949 1449 2356 199
Size: 397 353 500 907 1739
Fragments arranged by size:
1739 907 500 397 353
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PfoI T'CCnGG_A
Cuts at: 127 127
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 365 397 3035 3067 365
Size: 32 2638 32 1194
Fragments arranged by size:
2638 1194 32 32
PsiI TTA'TAA
Cuts at: 305 305
Size: 3896
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 770 802 770
Size: 32 3864
PstI C_TGCA'G
Cuts at: 618 943 1628 618
Size: 325 685 2886
Fragments arranged by size:
2886 685 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 619 1321 1681 619
Size: 702 360 2834
Fragments arranged by size:
2834 702 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 695 3413 695
Size: 2718 1178
SfcI C'TryA_G
Cuts at: 33 614 939 1624 2617 2808 33
Size: 581 325 685 993 191 1121
Fragments arranged by size:
1121 993 685 581 325 191
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2458 2720 1051
Size: 915 492 262 2227
Fragments arranged by size:
2227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 179 707 1030 1384 179
Size: 528 323 354 2691
Fragments arranged by size:
2691 528 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 512 693 3411 512
Size: 181 2718 997
Fragments arranged by size:
2718 997 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI