Restriction map for
pF1KB6328
(Circular) (Six-base) MAPSORT of: pF1KB6328.seq Check: 9759 from: 1 to: 3806
pF1KB6328 3806 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 844 844
Size: 3806
Acc65I G'GTAC_C
Cuts at: 822 822
Size: 3806
AclI AA'CG_TT
Cuts at: 3646 3646
Size: 3806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 222 1631 2063 2810 3078 147
Size: 75 1409 432 747 268 875
Fragments arranged by size:
1409 875 747 432 268 75
AflII C'TTAA_G
Cuts at: 352 352
Size: 3806
AflIII A'CryG_T
Cuts at: 1158 2262 3239 1158
Size: 1104 977 1725
Fragments arranged by size:
1725 1104 977
AgeI A'CCGG_T
Cuts at: 2194 2194
Size: 3806
AlwNI CAG_nnn'CTG
Cuts at: 292 1111 2678 292
Size: 819 1567 1420
Fragments arranged by size:
1567 1420 819
ApaLI G'TGCA_C
Cuts at: 2576 2576
Size: 3806
ApoI r'AATT_y
Cuts at: 810 3159 3415 810
Size: 2349 256 1201
Fragments arranged by size:
2349 1201 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
AvaI C'yCGr_G
Cuts at: 797 826 797
Size: 29 3777
AvrII C'CTAG_G
Cuts at: 709 709
Size: 3806
BamHI G'GATC_C
Cuts at: 831 831
Size: 3806
BanI G'GyrC_C
Cuts at: 822 1236 1484 1519 822
Size: 414 248 35 3109
Fragments arranged by size:
3109 414 248 35
BanII G_rGCy'C
Cuts at: 448 820 1850 448
Size: 372 1030 2404
Fragments arranged by size:
2404 1030 372
BbeI G_GCGC'C
Cuts at: 1240 1488 1240
Size: 248 3558
BciVI GTATCCnnnnn_n'
Cuts at: 173 368 1704 2465 3806 173
Size: 195 1336 761 1341 173
Fragments arranged by size:
1341 1336 761 195 173
BclI T'GATC_A
Cuts at: 1326 1326
Size: 3806
BglII A'GATC_T
Cuts at: 1321 3358 1321
Size: 2037 1769
BlpI GC'TnA_GC
Cuts at: 918 918
Size: 3806
Bme1580I G_kGCm'C
Cuts at: 1431 1524 2580 1431
Size: 93 1056 2657
Fragments arranged by size:
2657 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 450 1018 1099 1434 3578 450
Size: 568 81 335 2144 678
Fragments arranged by size:
2144 678 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 3806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 722
Size: 3806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1861 2353 2651 982
Size: 879 492 298 2137
Fragments arranged by size:
2137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3510 3510
Size: 3806
BsaAI yAC'GTr
Cuts at: 1159 1159
Size: 3806
BsaBI GATnn'nnATC
Cuts at: 6 1320 3357 6
Size: 1314 2037 455
Fragments arranged by size:
2037 1314 455
BsaHI Gr'CG_yC
Cuts at: 1237 1485 2187 3683 1237
Size: 248 702 1496 1360
Fragments arranged by size:
1496 1360 702 248
BsaWI w'CCGG_w
Cuts at: 791 995 1516 2194 2468 2615 3026 791
Size: 204 521 678 274 147 411 1571
Fragments arranged by size:
1571 678 521 411 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 430
Size: 30 3776
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 188 649 188
Size: 461 3345
BseYI C'CCAG_C
Cuts at: 569 1231 1989 2566 3062 569
Size: 662 758 577 496 1313
Fragments arranged by size:
1313 758 662 577 496
BsiEI CG_ry'CG
Cuts at: 104 1348 1394 2602 104
Size: 1244 46 1208 1308
Fragments arranged by size:
1308 1244 1208 46
BsiHKAI G_wGCw'C
Cuts at: 820 1598 1788 2580 820
Size: 778 190 792 2046
Fragments arranged by size:
2046 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 448 820 1431 1524 1598 1788 1850 2580 448
Size: 372 611 93 74 190 62 730 1674
Fragments arranged by size:
1674 730 611 372 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3026 3026
Size: 3806
BspHI T'CATG_A
Cuts at: 2982 2982
Size: 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 856 1070 1372 1753 856
Size: 214 302 381 2909
Fragments arranged by size:
2909 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2098 2152 3636 3801 50
Size: 2048 54 1484 165 55
Fragments arranged by size:
2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1718 1718
Size: 3806
BsrFI r'CCGG_y
Cuts at: 1804 1985 2194 1804
Size: 181 209 3416
Fragments arranged by size:
3416 209 181
BssSI C'ACGA_G
Cuts at: 2077 2435 2077
Size: 358 3448
BstAPI GCAn_nnn'nTGC
Cuts at: 1183 1183
Size: 3806
BstBI TT'CG_AA
Cuts at: 2167 2167
Size: 3806
BstYI r'GATC_y
Cuts at: 1 380 831 1298 1321 1656 1902 2914 3000
Size: 379 451 467 23 335 246 1012 86
Cuts at: 3000 3012 3358 1
Size: 12 346 449
Fragments arranged by size:
1012 467 451 449 379 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1756 1937 2100 1756
Size: 181 163 3462
Fragments arranged by size:
3462 181 163
BtsI GCAGTG_nn'
Cuts at: 1049 1049
Size: 3806
DraI TTT'AAA
Cuts at: 805 805
Size: 3806
DraIII CAC_nnn'GTG
Cuts at: 310 310
Size: 3806
DrdI GACnn_nn'nnGTC
Cuts at: 1512 2370 1512
Size: 858 2948
EaeI y'GGCC_r
Cuts at: 1391 1565 1956 1983 3328 1391
Size: 174 391 27 1345 1869
Fragments arranged by size:
1869 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1391 1391
Size: 3806
EarI CTCTTCn'nnn_
Cuts at: 130 1829 2039 130
Size: 1699 210 1897
Fragments arranged by size:
1897 1699 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2324 2470 3616 2014
Size: 310 146 1146 2204
Fragments arranged by size:
2204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 818 818
Size: 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 222 722 1631 2063 2810 3078 147
Size: 75 500 909 432 747 268 875
Fragments arranged by size:
909 875 747 500 432 268 75
EcoO109I rG'GnC_Cy
Cuts at: 251 289 945 251
Size: 38 656 3112
Fragments arranged by size:
3112 656 38
EcoRI G'AATT_C
Cuts at: 810 810
Size: 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3806
FspI TGC'GCA
Cuts at: 1587 1587
Size: 3806
HaeII r_GCGC'y
Cuts at: 642 1240 1488 2510 3489 642
Size: 598 248 1022 979 959
Fragments arranged by size:
1022 979 959 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 709 741 1648 1678 1680 1710 430
Size: 32 247 32 907 30 2 30 2526
Fragments arranged by size:
2526 907 247 32 32 30 30 2
HincII GTy'rAC
Cuts at: 845 845
Size: 3806
KasI G'GCGC_C
Cuts at: 1236 1484 1236
Size: 248 3558
KpnI G_GTAC'C
Cuts at: 826 826
Size: 3806
MluI A'CGCG_T
Cuts at: 3239 3239
Size: 3806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2661 2477
Size: 184 3622
MscI TGG'CCA
Cuts at: 1567 1567
Size: 3806
MslI CAynn'nnrTG
Cuts at: 598 1795 1922 598
Size: 1197 127 2482
Fragments arranged by size:
2482 1197 127
MspA1I CmG'CkG
Cuts at: 917 1231 1591 2604 2849 917
Size: 314 360 1013 245 1874
Fragments arranged by size:
1874 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1987 1987
Size: 3806
NarI GG'CG_CC
Cuts at: 1237 1485 1237
Size: 248 3558
NgoMIV G'CCGG_C
Cuts at: 1985 1985
Size: 3806
NspI r_CATG'y
Cuts at: 859 1359 2266 859
Size: 500 907 2399
Fragments arranged by size:
2399 907 500
PciI A'CATG_T
Cuts at: 2262 2262
Size: 3806
PfoI T'CCnGG_A
Cuts at: 147 764 147
Size: 617 3189
PmeI GTTT'AAAC
Cuts at: 805 805
Size: 3806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3774
PpuMI rG'GwC_Cy
Cuts at: 289 289
Size: 3806
PstI C_TGCA'G
Cuts at: 853 1538 853
Size: 685 3121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3806
PvuII CAG'CTG
Cuts at: 1231 1591 1231
Size: 360 3446
RsrII CG'GwC_CG
Cuts at: 2001 2001
Size: 3806
SacI G_AGCT'C
Cuts at: 820 820
Size: 3806
SalI G'TCGA_C
Cuts at: 843 843
Size: 3806
SapI GCTCTTCn'nnn_
Cuts at: 1829 2039 1829
Size: 210 3596
SbfI CC_TGCA'GG
Cuts at: 853 853
Size: 3806
ScaI AGT'ACT
Cuts at: 3323 3323
Size: 3806
SexAI A'CCwGG_T
Cuts at: 306 306
Size: 3806
SfcI C'TryA_G
Cuts at: 33 562 677 849 1534 2527 2718 33
Size: 529 115 172 685 993 191 1121
Fragments arranged by size:
1121 993 685 529 191 172 115
SfoI GGC'GCC
Cuts at: 1238 1486 1238
Size: 248 3558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
SmaI CCC'GGG
Cuts at: 799 828 799
Size: 29 3777
SmlI C'TyrA_G
Cuts at: 352 961 1876 2368 2630 352
Size: 609 915 492 262 1528
Fragments arranged by size:
1528 915 609 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3806
SphI G_CATG'C
Cuts at: 859 1359 859
Size: 500 3306
StyI C'CwwG_G
Cuts at: 709 940 1294 709
Size: 231 354 3221
Fragments arranged by size:
3221 354 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1389 1522 1981 2190 1389
Size: 133 459 209 3005
Fragments arranged by size:
3005 459 209 133
TatI w'GTAC_w
Cuts at: 3321 3321
Size: 3806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 3774
Tth111I GACn'n_nGTC
Cuts at: 1603 1603
Size: 3806
XbaI T'CTAG_A
Cuts at: 837 837
Size: 3806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 437 547 1301 437
Size: 110 754 2942
Fragments arranged by size:
2942 754 110
XmaI C'CCGG_G
Cuts at: 797 826 797
Size: 29 3777
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI