Restriction map for
pF1KB6329
(Circular) (Six-base) MAPSORT of: pF1KB6329.seq Check: 8415 from: 1 to: 3896
pF1KB6329 3896 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 741 741
Size: 3896
AccI GT'mk_AC
Cuts at: 488 934 488
Size: 446 3450
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AflIII A'CryG_T
Cuts at: 491 854 1248 2352 3329 491
Size: 363 394 1104 977 1058
Fragments arranged by size:
1104 1058 977 394 363
AgeI A'CCGG_T
Cuts at: 768 2284 768
Size: 1516 2380
AhdI GACnn_n'nnGTC
Cuts at: 183 183
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 595 1201 2768 595
Size: 606 1567 1723
Fragments arranged by size:
1723 1567 606
ApaI G_GGCC'C
Cuts at: 160 630 160
Size: 470 3426
ApaLI G'TGCA_C
Cuts at: 412 2666 412
Size: 2254 1642
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 152 916 152
Size: 764 3132
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 285 310 912 1326 1574 1609 285
Size: 25 602 414 248 35 2572
Fragments arranged by size:
2572 602 414 248 35 25
BanII G_rGCy'C
Cuts at: 160 542 630 809 910 1940 160
Size: 382 88 179 101 1030 2116
Fragments arranged by size:
2116 1030 382 179 101 88
BbeI G_GCGC'C
Cuts at: 289 314 1330 1578 289
Size: 25 1016 248 2607
Fragments arranged by size:
2607 1016 248 25
BbsI GAAGACnn'nnnn_
Cuts at: 645 738 645
Size: 93 3803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 629 663 629
Size: 34 3862
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 625 625
Size: 3896
BglII A'GATC_T
Cuts at: 377 1411 3448 377
Size: 1034 2037 825
Fragments arranged by size:
2037 1034 825
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 160 416 630 1521 1614 2670 160
Size: 256 214 891 93 1056 1386
Fragments arranged by size:
1386 1056 891 256 214 93
BmgBI CAC'GTC
Cuts at: 855 855
Size: 3896
BmrI ACTGGGnnnn_n'
Cuts at: 325 583 1108 1189 1524 3668 325
Size: 258 525 81 335 2144 553
Fragments arranged by size:
2144 553 525 335 258 81
BmtI G_CTAG'C
Cuts at: 569 569
Size: 3896
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
Bpu10I CC'TnA_GC
Cuts at: 320 682 868 320
Size: 362 186 3348
Fragments arranged by size:
3348 362 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 692 3600 397
Size: 295 2908 693
Fragments arranged by size:
2908 693 295
BsaAI yAC'GTr
Cuts at: 492 672 1249 492
Size: 180 577 3139
Fragments arranged by size:
3139 577 180
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 286 311 1327 1575 2277 3773 286
Size: 25 1016 248 702 1496 409
Fragments arranged by size:
1496 1016 702 409 248 25
BsaWI w'CCGG_w
Cuts at: 768 1085 1606 2284 2558 2705 3116 768
Size: 317 521 678 274 147 411 1548
Fragments arranged by size:
1548 678 521 411 317 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 316 808 160
Size: 156 492 3248
Fragments arranged by size:
3248 492 156
BseYI C'CCAG_C
Cuts at: 1321 2079 2656 3152 1321
Size: 758 577 496 2065
Fragments arranged by size:
2065 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 122 1438 1484 2692 104
Size: 18 1316 46 1208 1308
Fragments arranged by size:
1316 1308 1208 46 18
BsiHKAI G_wGCw'C
Cuts at: 416 542 716 809 869 910 1688 1878 2670
Size: 126 174 93 60 41 778 190 792
Cuts at: 2670 416
Size: 1642
Fragments arranged by size:
1642 792 778 190 174 126 93 60
41
Bsp1286I G_dGCh'C
Cuts at: 160 416 542 630 716 809 869 910 1521
Size: 256 126 88 86 93 60 41 611
Cuts at: 1521 1614 1688 1878 1940 2670 160
Size: 93 74 190 62 730 1386
Fragments arranged by size:
1386 730 611 256 190 126 93 93
88 86 74 62 60 41
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 350 608 710 741 946 1160 1462 1843 350
Size: 258 102 31 205 214 302 381 2403
Fragments arranged by size:
2403 381 302 258 214 205 102 31
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 686 768 1894 2075 2284 686
Size: 82 1126 181 209 2298
Fragments arranged by size:
2298 1126 209 181 82
BssSI C'ACGA_G
Cuts at: 777 2167 2525 777
Size: 1390 358 2148
Fragments arranged by size:
2148 1390 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstEII G'GTnAC_C
Cuts at: 175 175
Size: 3896
BstYI r'GATC_y
Cuts at: 1 377 921 1388 1411 1746 1992 3004 3090
Size: 376 544 467 23 335 246 1012 86
Cuts at: 3090 3102 3448 1
Size: 12 346 449
Fragments arranged by size:
1012 544 467 449 376 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 350 350
Size: 3896
BtgI C'CryG_G
Cuts at: 107 877 107
Size: 770 3126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 560 1139 560
Size: 579 3317
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 119 880 1481 1655 2046 2073 3418 119
Size: 761 601 174 391 27 1345 597
Fragments arranged by size:
1345 761 601 597 391 174 27
EagI C'GGCC_G
Cuts at: 119 1481 119
Size: 1362 2534
EarI CTCTTCn'nnn_
Cuts at: 238 1919 2129 238
Size: 1681 210 2005
Fragments arranged by size:
2005 1681 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 540 807 908 540
Size: 267 101 3528
Fragments arranged by size:
3528 267 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 156 264 617 678 1035 156
Size: 108 353 61 357 3017
Fragments arranged by size:
3017 357 353 108 61
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 289 314 1330 1578 2600 3579 289
Size: 25 1016 248 1022 979 606
Fragments arranged by size:
1022 1016 979 606 248 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 844 876 1738 1768 1770 1800 169
Size: 32 643 32 862 30 2 30 2265
Fragments arranged by size:
2265 862 643 32 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 285 310 1326 1574 285
Size: 25 1016 248 2607
Fragments arranged by size:
2607 1016 248 25
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 457 512 1885 2012 457
Size: 55 1373 127 2341
Fragments arranged by size:
2341 1373 127 55
MspA1I CmG'CkG
Cuts at: 162 281 1007 1321 1681 2694 2939 162
Size: 119 726 314 360 1013 245 1119
Fragments arranged by size:
1119 1013 726 360 314 245 119
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 286 311 1327 1575 286
Size: 25 1016 248 2607
Fragments arranged by size:
2607 1016 248 25
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NheI G'CTAG_C
Cuts at: 565 565
Size: 3896
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PfoI T'CCnGG_A
Cuts at: 719 719
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 678 678
Size: 3896
PshAI GACnn'nnGTC
Cuts at: 331 331
Size: 3896
PspOMI G'GGCC_C
Cuts at: 156 626 156
Size: 470 3426
PstI C_TGCA'G
Cuts at: 527 943 1628 527
Size: 416 685 2795
Fragments arranged by size:
2795 685 416
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 542 809 910 542
Size: 267 101 3528
Fragments arranged by size:
3528 267 101
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SexAI A'CCwGG_T
Cuts at: 482 482
Size: 3896
SfcI C'TryA_G
Cuts at: 33 523 939 1624 2617 2808 33
Size: 490 416 685 993 191 1121
Fragments arranged by size:
1121 993 685 490 416 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 625 625
Size: 3896
SfoI GGC'GCC
Cuts at: 287 312 1328 1576 287
Size: 25 1016 248 2607
Fragments arranged by size:
2607 1016 248 25
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2458 2720 1051
Size: 915 492 262 2227
Fragments arranged by size:
2227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 107 356 1030 1384 107
Size: 249 674 354 2619
Fragments arranged by size:
2619 674 354 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 857 3411 857
Size: 2554 1342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BpmI BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PmlI PsiI
PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI