Restriction map for
pF1KB6336
(Circular) (Six-base) MAPSORT of: pF1KB6336.seq Check: 6906 from: 1 to: 3983
pF1KB6336 3983 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 733 733
Size: 3983
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 651 3823 651
Size: 3172 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 748 1288 2855 748
Size: 540 1567 1876
Fragments arranged by size:
1876 1567 540
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 384 487 571 987 3336 3592 384
Size: 103 84 416 2349 256 775
Fragments arranged by size:
2349 775 416 256 103 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 1003 1003
Size: 3983
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 999 1413 1661 1696 999
Size: 414 248 35 3286
Fragments arranged by size:
3286 414 248 35
BanII G_rGCy'C
Cuts at: 997 2027 997
Size: 1030 2953
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2757 1608
Size: 93 1056 2834
Fragments arranged by size:
2834 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 536 1497 3534 6
Size: 530 961 2037 455
Fragments arranged by size:
2037 961 530 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 177 1172 1693 2371 2645 2792 3203 177
Size: 995 521 678 274 147 411 957
Fragments arranged by size:
995 957 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 313 883 970 196
Size: 117 570 87 3209
Fragments arranged by size:
3209 570 117 87
BseYI C'CCAG_C
Cuts at: 1408 2166 2743 3239 1408
Size: 758 577 496 2152
Fragments arranged by size:
2152 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 3983
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2779 104
Size: 1421 46 1208 1308
Fragments arranged by size:
1421 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 997 1775 1965 2757 997
Size: 778 190 792 2223
Fragments arranged by size:
2223 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 997 1608 1701 1775 1965 2027 2757 997
Size: 611 93 74 190 62 730 2223
Fragments arranged by size:
2223 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3203 3203
Size: 3983
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 1033 1247 1549 1930 733
Size: 300 214 302 381 2786
Fragments arranged by size:
2786 381 302 300 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 1981 2162 2371 1981
Size: 181 209 3593
Fragments arranged by size:
3593 209 181
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 604 2344 604
Size: 1740 2243
BstXI CCAn_nnnn'nTGG
Cuts at: 389 389
Size: 3983
BstYI r'GATC_y
Cuts at: 1 1008 1475 1498 1833 2079 3091 3177 3189
Size: 1007 467 23 335 246 1012 86 12
Cuts at: 3189 3535 1
Size: 346 449
Fragments arranged by size:
1012 1007 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1933 2114 2277 1933
Size: 181 163 3639
Fragments arranged by size:
3639 181 163
BtsI GCAGTG_nn'
Cuts at: 427 454 1226 427
Size: 27 772 3184
Fragments arranged by size:
3184 772 27
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 784 1568 1742 2133 2160 3505 784
Size: 784 174 391 27 1345 1262
Fragments arranged by size:
1345 1262 784 391 174 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 349 443 679 860 2006 2216 349
Size: 94 236 181 1146 210 2116
Fragments arranged by size:
2116 1146 236 210 181 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 325 2191 2501 2647 3793 325
Size: 1866 310 146 1146 515
Fragments arranged by size:
1866 1146 515 310 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1122 1122
Size: 3983
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 487 519 579 611 752 784 1825
Size: 32 283 32 60 32 141 32 1041
Cuts at: 1825 1855 1857 1887 172
Size: 30 2 30 2268
Fragments arranged by size:
2268 1041 283 141 60 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 408 1022 408
Size: 614 3369
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2654 2838 2654
Size: 184 3799
MscI TGG'CCA
Cuts at: 786 1744 786
Size: 958 3025
MslI CAynn'nnrTG
Cuts at: 251 581 737 1972 2099 251
Size: 330 156 1235 127 2135
Fragments arranged by size:
2135 1235 330 156 127
MspA1I CmG'CkG
Cuts at: 1094 1408 1768 2781 3026 1094
Size: 314 360 1013 245 2051
Fragments arranged by size:
2051 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2164 2164
Size: 3983
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 2162 2162
Size: 3983
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PstI C_TGCA'G
Cuts at: 201 1030 1715 201
Size: 829 685 2469
Fragments arranged by size:
2469 829 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 1408 1768 1408
Size: 360 3623
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 802 3500 802
Size: 2698 1285
SexAI A'CCwGG_T
Cuts at: 718 718
Size: 3983
SfcI C'TryA_G
Cuts at: 33 197 1026 1711 2704 2895 33
Size: 164 829 685 993 191 1121
Fragments arranged by size:
1121 993 829 685 191 164
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StyI C'CwwG_G
Cuts at: 107 1117 1471 107
Size: 1010 354 2619
Fragments arranged by size:
2619 1010 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1566 1699 2158 2367 1566
Size: 133 459 209 3182
Fragments arranged by size:
3182 459 209 133
TatI w'GTAC_w
Cuts at: 258 800 3498 258
Size: 542 2698 743
Fragments arranged by size:
2698 743 542
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI