Restriction map for
pF1KB6340
(Circular) (Six-base) MAPSORT of: pF1KB6340.seq Check: 4551 from: 1 to: 3983
pF1KB6340 3983 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1808 2240 2987 3255 352
Size: 1456 432 747 268 1080
Fragments arranged by size:
1456 1080 747 432 268
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 675 721 861 1288 2855 675
Size: 46 140 427 1567 1803
Fragments arranged by size:
1803 1567 427 140 46
ApaI G_GGCC'C
Cuts at: 853 853
Size: 3983
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 943 20
Size: 923 3060
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 700 1003 700
Size: 303 3680
BamHI G'GATC_C
Cuts at: 665 1008 665
Size: 343 3640
BanI G'GyrC_C
Cuts at: 969 999 1413 1661 1696 969
Size: 30 414 248 35 3256
Fragments arranged by size:
3256 414 248 35 30
BanII G_rGCy'C
Cuts at: 212 530 625 804 853 997 2027 212
Size: 318 95 179 49 144 1030 2168
Fragments arranged by size:
2168 1030 318 179 144 95 49
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbvCI CC'TCA_GC
Cuts at: 257 257
Size: 3983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 643 677 643
Size: 34 3949
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 533 1503 533
Size: 970 3013
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 139 1095 139
Size: 956 3027
Bme1580I G_kGCm'C
Cuts at: 853 1608 1701 2757 853
Size: 755 93 1056 2079
Fragments arranged by size:
2079 1056 755 93
BmgBI CAC'GTC
Cuts at: 348 348
Size: 3983
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 196 838 154
Size: 42 642 3299
Fragments arranged by size:
3299 642 42
Bpu10I CC'TnA_GC
Cuts at: 257 257
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1159 2038 2530 2828 577
Size: 582 879 492 298 1732
Fragments arranged by size:
1732 879 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 538 1497 3534 6
Size: 532 959 2037 455
Fragments arranged by size:
2037 959 532 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 494 585 926 1172 1693 2371 2645 2792 3203
Size: 91 341 246 521 678 274 147 411
Cuts at: 3203 494
Size: 1274
Fragments arranged by size:
1274 678 521 411 341 274 246 147
91
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 276 326 370 373 394 754 196
Size: 80 50 44 3 21 360 3425
Fragments arranged by size:
3425 360 80 50 44 21 3
BseYI C'CCAG_C
Cuts at: 126 391 675 854 1408 2166 2743 3239 126
Size: 265 284 179 554 758 577 496 870
Fragments arranged by size:
870 758 577 554 496 284 265 179
BsiEI CG_ry'CG
Cuts at: 104 158 1525 1571 2779 104
Size: 54 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 54 46
BsiHKAI G_wGCw'C
Cuts at: 212 530 997 1775 1965 2757 212
Size: 318 467 778 190 792 1438
Fragments arranged by size:
1438 792 778 467 318 190
Bsp1286I G_dGCh'C
Cuts at: 212 530 625 804 853 997 1608 1701 1775
Size: 318 95 179 49 144 611 93 74
Cuts at: 1775 1965 2027 2757 212
Size: 190 62 730 1438
Fragments arranged by size:
1438 730 611 318 190 179 144 95
93 74 62 49
BspEI T'CCGG_A
Cuts at: 585 3203 585
Size: 2618 1365
BspHI T'CATG_A
Cuts at: 903 3159 903
Size: 2256 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 647 2275 2329 3813 3978 50
Size: 597 1628 54 1484 165 55
Fragments arranged by size:
1628 1484 597 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 608 966 1981 2162 2371 608
Size: 358 1015 181 209 2220
Fragments arranged by size:
2220 1015 358 209 181
BsrGI T'GTAC_A
Cuts at: 686 686
Size: 3983
BssSI C'ACGA_G
Cuts at: 529 2254 2612 529
Size: 1725 358 1900
Fragments arranged by size:
1900 1725 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 414 414
Size: 3983
BstYI r'GATC_y
Cuts at: 1 665 1008 1475 1498 1833 2079 3091 3177
Size: 664 343 467 23 335 246 1012 86
Cuts at: 3177 3189 3535 1
Size: 12 346 449
Fragments arranged by size:
1012 664 467 449 346 343 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 525 1933 2114 2277 525
Size: 1408 181 163 2231
Fragments arranged by size:
2231 1408 181 163
BtsI GCAGTG_nn'
Cuts at: 1226 1226
Size: 3983
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 475 1689 2547 475
Size: 1214 858 1911
Fragments arranged by size:
1911 1214 858
EaeI y'GGCC_r
Cuts at: 610 770 950 1568 1742 2133 2160 3505 610
Size: 160 180 618 174 391 27 1345 1088
Fragments arranged by size:
1345 1088 618 391 180 174 160 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 925 2006 2216 925
Size: 1081 210 2692
Fragments arranged by size:
2692 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 2191 2501 2647 3793 163
Size: 2028 310 146 1146 353
Fragments arranged by size:
2028 1146 353 310 146
EcoICRI GAG'CTC
Cuts at: 210 528 995 210
Size: 318 467 3198
Fragments arranged by size:
3198 467 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 196 352 838 1808 2240 2987 3255 154
Size: 42 156 486 970 432 747 268 882
Fragments arranged by size:
970 882 747 486 432 268 156 42
EcoO109I rG'GnC_Cy
Cuts at: 552 681 718 849 850 1122 552
Size: 129 37 131 1 272 3413
Fragments arranged by size:
3413 272 131 129 37 1
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 936 2002 936
Size: 1066 2917
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 885 917 1825 1855 1857 1887 885
Size: 32 908 30 2 30 2981
Fragments arranged by size:
2981 908 32 30 30 2
HincII GTy'rAC
Cuts at: 480 1022 480
Size: 542 3441
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2654 2838 2654
Size: 184 3799
MscI TGG'CCA
Cuts at: 952 1744 952
Size: 792 3191
MslI CAynn'nnrTG
Cuts at: 406 1972 2099 406
Size: 1566 127 2290
Fragments arranged by size:
2290 1566 127
MspA1I CmG'CkG
Cuts at: 579 861 888 1094 1408 1768 2781 3026 579
Size: 282 27 206 314 360 1013 245 1536
Fragments arranged by size:
1536 1013 360 314 282 245 206 27
NaeI GCC'GGC
Cuts at: 2164 2164
Size: 3983
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 2162 2162
Size: 3983
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PasI CC'CwG_GG
Cuts at: 804 804
Size: 3983
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PflMI CCAn_nnn'nTGG
Cuts at: 675 675
Size: 3983
PfoI T'CCnGG_A
Cuts at: 668 668
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 3122 3154 631
Size: 32 2459 32 1460
Fragments arranged by size:
2459 1460 32 32
PpuMI rG'GwC_Cy
Cuts at: 681 681
Size: 3983
PspOMI G'GGCC_C
Cuts at: 849 849
Size: 3983
PstI C_TGCA'G
Cuts at: 197 893 1030 1715 197
Size: 696 137 685 2465
Fragments arranged by size:
2465 696 685 137
PvuI CG_AT'CG
Cuts at: 104 158 104
Size: 54 3929
PvuII CAG'CTG
Cuts at: 579 861 1408 1768 579
Size: 282 547 360 2794
Fragments arranged by size:
2794 547 360 282
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 212 530 997 212
Size: 318 467 3198
Fragments arranged by size:
3198 467 318
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 193 889 1026 1711 2704 2895 33
Size: 160 696 137 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 191 160 137
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 702 1005 702
Size: 303 3680
SmlI C'TyrA_G
Cuts at: 556 1138 2053 2545 2807 556
Size: 582 915 492 262 1732
Fragments arranged by size:
1732 915 582 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StuI AGG'CCT
Cuts at: 199 199
Size: 3983
StyI C'CwwG_G
Cuts at: 107 1117 1471 107
Size: 1010 354 2619
Fragments arranged by size:
2619 1010 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 1566 1699 2158 2367 367
Size: 1199 133 459 209 1983
Fragments arranged by size:
1983 1199 459 209 133
TatI w'GTAC_w
Cuts at: 686 3498 686
Size: 2812 1171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 870 1014 870
Size: 144 3839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 259 1478 259
Size: 1219 2764
XmaI C'CCGG_G
Cuts at: 700 1003 700
Size: 303 3680
XmnI GAAnn'nnTTC
Cuts at: 301 301
Size: 3983
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BfrBI BglI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI